1.Effect of verapamil on the alteration of phospholipase A_2 activity in acute pancreatitis rats
Xinze ZHOU ; Qinsheng MAO ; Yuquan CHEN ;
Chinese Journal of General Surgery 1997;0(04):-
Objective To evaluate activity alteration of the pancreatic tissue phospholipase A 2 (PLA 2) and the therapeutic effects of verapamil in acute pancreatitis (AP) rats. Methods After the model of AP in the rat was established by the closed duodenal loop technique, the changes of PLA 2 activity were observed, and the pancreatic histology was examined by light and electron microscopy. Results At 16 and 24 hours after induction of AP in rats, significant inhibition of PLA 2 avtivity in pancreatic tissue was shown in the treated group (32.34?3.87, 35.26?4.52) as compared with AP group (44.83?5.31,47.77?5.86). The treated animals also showed decreasing in the severity of pancreatic pathology. Conclusions In AP rats exist high activity of PLA 2. Verapamil, a calcium channel blocker, might have therapeutic effects on AP by inhibiting the activity of PLA 2.
2.Emodin in the treatment of acute pancreatitis in rats
Xinze ZHOU ; Yong CAO ; Wenyi ZHANG ; Xiaojian ZHANG ; Jing KE
Chinese Journal of Digestive Surgery 2008;7(6):416-418
Objective To investigate the efficacy of emodin in the treatment of acute pancreatitis in rats.Methods Forty-five SD rats were divided into control group,pancreatitis group and treatment group.The apoptotic index of pancreatic acinar cells.the expression of NF-κB mRNA and Bax mRNA,the apoptosis rate of peripheral polymorphonuclear neutrophil(PMN),and the expression of PMN caspase-3 and PMN caspase-8 were detected.Results Compared with the panereatitis group,the apoptotic index of pancreatic acinax cells and PMN,and the expressions of NF-κB mRNA,Bax mRNA.PMN caspase-3 and PMN caspase-8 in pancreas tissue were significantly increased in the treatment group(F=853.199,327.126,143.586,48.857,231.750,96.552,P<0.05).The histopathologic score of panereatitis Was negatively correlated with the apoptotic index of the pancreatic acinar cells and PMN(r=-0.96,-0.94,P<0.05).The apeptotic index ofthe pancreatic acinar cells Was positively correlated with the expression of NF-κB mRNA,Bax mRNA in pancreas tissue(r=0.73,0.76,P<0.05).The expression of NF-κB mRNA was positively correlated withthat of Bax mRNA(r=0.94,P<0.05).The apoptotie index of PMN was positively correlated with the expression of PMN caspase-3 and PMN caspase-8 (r=0.99,0.99.P<0.05).Conclusions Emodin alleviates acute pancreatitis via regulating the apoptosis of pancreatic acinar cells through Bax pathway,and regulating the PMN apoptosis through caspase pathway.
3.Blockade of intrathoracic lymphatic flow significantly ameliorates intestinal bacterial translocation in rats with obstructive jaundice
Xinze ZHOU ; Qinshen MAO ; Yaohui NI ; Ruixin CHEN ; Xiu YU ; Liping LU
Chinese Journal of General Surgery 1997;0(04):-
Objective To investigate the effect of lymphatic flow impedement on intestinal bacterial translocation in rats with obstructive jaundice. Methods Murine obstructive jaundice model was established by ligating lower choledochus. Sixty rats were divided into three groups: sham-operative group (group A), obstructive jaundice group (group B), and obstructive jaundice with thoracic duct ligation group (group C). At post-op day 15 vena cava inferior blood? portal vein blood were drawn, and endotoxin, TNF-?, IL-6 were determined. Baterial culture of mesenteric lymph nodes and lung were measured with aseptic manipulation. The extreme intestinal lumen mucosa and lung were studied pathologically. Results Blood concentration of endotoxin increased in group B and C ( P
4.Eicosanoid metabolic disturbance and the role of calcium channel bocker in murine acute pancreatitis
Xinze ZHOU ; Qinshen MAO ; Yiaohui NI ; Xiu YU ; Ruixin CHEN ; Liping LU ; Yuquan CHEN
Chinese Journal of General Surgery 2001;0(07):-
Objective To evaluate the alterations of thromboxane (TXB_2)、 prostacyclin (PGI_2)in murine acute pancreatitis (AP) and the therapeutic effect of calcium channel bocker-verapamil. MethodsA rat AP model was established to observe the alterations of plasma levels of TXB_2, 6-keto-PGF_ 1? and TXB_2/6-keto-PGF_ 1? ratio and the effect of verapamil. Pancreatic histology was examined by light and electron microscopy. ResultsAt 16 and 24 hours after induction of AP, the plasma levels of TXB_2[(1112?235)pg/ml、(1265?162)pg/ml] and TXB_2/6-keto-PGF_ 1? ratio(9.9?0.9,10.2?1.3)increased significantly. With varapamil therapy, the plasma levels of TXB_2[(671?102)pg/ml、(697?93)pg/ml] and TXB_2/6-keto-PGF_ 1? ratio (6.9?2.2)、(6.4?0.7) were dramatically lower than those in control group (P
5.Experimental study on preventive and therapeutic effec ts of 5 radioprotectants on combined radiation-burn injured mice
Chaohua GUO ; Peiyan KONG ; Zhongmin ZOU ; Yanhong ZHOU ; Jinming ZHOU ; Yong ZHANG ; Cunmeng SHI ; Xinze RAN ; Chengji LUO
Journal of Third Military Medical University 2001;23(5):544-546
Objective To compare the irradiation-protective and inter-synergestic effects of E838,WR-2721, Rubia cordifolia, cystamin e hydrochloride and ethinyl estradiol on radiation and combined radiation-burn injury. Methods Above-mentioned drugs were given to the mice i ntraperitoneally, or intragastrcally, then, the mortality and the average surviv al d for 30 d were observed before and after the administration of the drug s. Results ①When drugs were before injury , the survival rate and the average survival d of the radiation and combined radiation-burn injured mice were increased obviously with the best effect in E838 and WR-2721. ②When drugs were given after injury, E838 and R. cordifolia also kept the effect. ③Combined appling WR-2721(pre) and E838(post)displayed a significant syner gistic reaction. Conclusion E838 and WR-2721 are more e ffective than the others in the prevention of radiation.
6.Feasibility of MSCs mobilization by G-CSF and its prosthetic effect in traumatic brain injury
Jun DENG ; Guoping AI ; Taoli ZHOU ; Junping WANG ; Hui XU ; Zhongmin ZOU ; Shiwu DONG ; Lei HAO ; Xinze RAN ; Yongping SU
Journal of Third Military Medical University 2002;0(12):-
Objective To explore the feasibility of mobilization circulating MSCs by G-CSF and observe the repairing effect of G-CSF mobilization in severe mouse traumatic brain injury(TBI) model.Methods MSCs-derived bone marrow and peripheral blood(PB) were cultured and its CFU-F were counted after mobilization by G-CSF.At 2,24,48,96,120,144,192,264,336 h after severe TBI in mice was establish,the neurobehavior of mice was measured by neurological examination and motor functional test,and mortality rate and pathologic changes were analyzed.Results MSCs-derived PB were successfully cultured.The CFU-F of mobilization group increased significantly than that of control group(P
7.The therapeutic effect of G-CSF-mobilized autologous stem cells on radiation pulmonary injury in mice
Huiqin SUN ; Tao WANG ; Xueli PANG ; Yu WANG ; Fengchao WANG ; Jun HE ; Qing ZHOU ; Shuang LONG ; Xinze RAN ; Yongping SU
Chinese Journal of Radiological Medicine and Protection 2019;39(3):178-184
Objective To investigate the effects of G-CSF-mobilized autologous stem cells in the prevention of radiation pulmonary injury.Methods Mice were divided into control group,irradiation group and treatment group.Mouse model of pulmonary fibrosis was established by exposing chest to a single dose of 14 Gy.Animals in the treatment group received recombinant human G-CSF (250 μg/kg daily for 5 d) before the irradiation in order to mobilize autologous stem cells in vivo.The general condition and mortality were documented after radiation injury.The pathological study with histological scoring,Masson staining and Sirius red staining with polarized light analysis were used to identify lung injury and the potential benefit of stem cell mobilization.Results Local chest irradiation of a single dose of 14 Gy was a suitable dose to create radiation-induced pulmonary fibrosis in mice.The death rate was 37.5%,which mainly happened around 11 weeks after injury.In contrast,all of the animals in G-CSF treated group survived.The ratio of lung to body mass was significantly increased in both irradiation group and treatment group (F =23.20,P<0.05) around 3 months after the injury,with a higher ratio in irradiation group than that in treatment group (P<0.05).Histological scoring for alveolar inflammation at 3 months after injury revealed statistically significant difference in irradiation group and treatment group compared with control group (F=11.93,P< 0.05).At this time point,the pathological observation showed lung tissue degeneration and necrosis with alveolitis and interstitial inflammation,as well as fibroblasts proliferation and focal collagen deposition in alveolar septa.At 4 month after the injury,the inflammation ininterstitial tissue was receded,but fibrosis and collagen deposition were significantly increased.In addition,at 3 and 4 months afterinjury,the pulmonary fibrosis was aggravated in irradiation group (F=28.73,16.85,P<0.05),and significantly alleviated in the treatment group (P<0.05).The similar results were confirmed in collagen content analysis (IOD) by Sirius red staining and image analysis (F =17.70,17.79,P< 0.05).Conclusions Autologous mobilization of stem cells could prevent the death of radiation-injured animals possibly by alleviating early lung injury and interstitial inflammation as well as the late pulmonary fibrosis,suggesting a therapeutic potential of autologous stem cell mobilization in radiation pulmonary fibrosis.