Objective To investigate the role of KAI1, intergrin ?5?1 and FAK proteins in occurrence and development and invasion and metastasis of lung cancer and their value in predicting the prognosis of lung cancer by analyzing the expression levels of KAI1, intergrin ?5?1 and FAK proteins in lung cancer and the relationship between them and clinicopathological parameters.Methods Lung cancer and normal lung tissue and metastasis cancer were detected and analyzed for their KAI1, and FAK protein level using immunobiochemical method. Results The positive rate of KAI1 in normal tissue, primary cancer and metastasis cancer was respectively 100.0, 24.7 and 0.0. The positive rate of intergrin?5?1 was respectively 0.0, 49.4 and 83.3. The positive rate of FAK was respectively 10.0,48.3 and 83.3. The difference of above three marker among three groups was significant (P

Purpose:Constructing a high-flux tissue microarray/tissue chip and detecting IGF-Ⅱprotein expression of cases by it, and to determine the correlation between IGF-Ⅱprotein expression and lung cancer. Methods:A series of tissue chips were prepared by using tissue arrayer with samples from lung cancers of different histological classifications. Specimens from 54 cases of lung cancer and 10 cases of normal lung tissues were detected immunohistochemically on a tissue chip for IGF-Ⅱprotein expression and its correlation to clinic-pathological parameters was analyzed statistically. Results:Positive rate of IGF-Ⅱprotein in lung cancer was 42.6%(23/54), which was higher than that of normal lung(0.0%, 0/10, P0.05). Conclusions:IGF-Ⅱprotein might be related to the malignant behaviors of lung cancer. Detecting the expression of IGF-Ⅱ protein probably can predict the prognosis of lung cancer. It is feasible to utilize tissue chip for screening of clinical tissue specimens on a large scale.

Background Acute carbon monoxide poisoning is now one of the major causes of morbidity and mortality in occupational and non-occupational poisoning in China, and become a serious public health problem. Understanding the current and predicting the future disease burden of carbon monoxide poisoning are essential for adopting effective disease prevention and control strategies in the future. Objective To understand the burden of disease of carbon monoxide poisoning in China from 1990 to 2019 and predict the trend of morbidity and mortality in the following decade to provide a scientific basis for the prevention of carbon monoxide poisoning in China. Methods The incidence, mortality and disability-adjusted-life-years (DALYs) rates of carbon monoxide poisoning in China from 1990 to 2019 were extracted from the Global Burden of Disease Database (GBD2019). Time trend analysis of the burden of disease for carbon monoxide poisoning was performed using Joinpoint 5.0.2 software, and annual percentage change (APC) and average annual percentage change (AAPC) and their 95%CIs were calculated. Bayesian age-period-cohort model (BAPC) and Nordpred age-period-cohort model were used in R 4.3.3 to predict the disease burden of carbon monoxide poisoning among Chinese residents in 2020—2029. Results An overall increasing trend of the age-standardized incidence rate of carbon monoxide poisoning in China from 1990 to 2019 was observed and AAPC=1.05%, of which the AAPC for women was 0.90% and that for men was 1.16%. An overall decreasing trend of the age-standardized mortality rate and age-standardized DALYs from 1990 to 2019 was observed and the AAPC for males or females was <0. the results of the BAPC model expected that an increasing trend from 2020 to 2029 in age-standardized morbidity rate (females: 18.85/100 000 in 2020 to 19.12/100,000 in 2029; males: 22.51/100 000 in 2020 to 22.60/100 000 in 2029); and a decreasing trend in age-standardized mortality rate (females: 0.70/100,000 in 2020 to 0.52/100 000 in 2029; males: 1.13/100 000 in 2020 to 0.97/100 000 in 2029). The results of the Nordpred age-period-cohort model showed similar trends, suggesting that the results were stable. Conclusion Carbon monoxide poisoning caused a serious burden of disease in the Chinese population during the period 1990—2019, with adolescents and middle-aged and elderly people being the most affected groups. Subsequent projections have shown an upward trend in age-standardized incidence rates and numbers of cases, so we still need to deepen the implementation of prevention and treatment of the occurrence and development of carbon monoxide poisoning, and to reduce the harm it causes to the population.

Objective To find out the incidence tendency and characteristics of bladder tumors during the past 28 yesrs in our institution. Methods The data from 1980 to 2007 were divided into three stages:1980-1989,1990-1999和2000-2007.Microsoft excel and SPSSl3.O were used to analyze the sex,age and histological types. Results There were 2350 cases of bladder tumors in 28 years.There were 92(66 males,26 females)cases of benign tumors and 2258(1788 males,470 females)malignant tumors,the incidence in male was 3.8 times higher than that in female.The cases of bladder malignant tumors increased,and the female increased faster than male.The peak age of incidence changed from 50-69 in 1980-1989 to 60-79 in 2000-2007.The average age of squamous cell carcinomas was 68 years,which was the oldest;and the average of paraganglioma was 35,which was the youngest.The age of urinary bladder carcinoma in 1980-1989,1990-1999 and 2000-2007 were significantly different(male:57.5±11. 7,62.6±12.3,65.9±11.3;female:58.7±13.6,60.75±12.1,65.85±12.0,respectively,P<0.05).In male,the age of squamous cell carcinomas was significantly different from the age of urothelial cell carcinomas and adenocarnomas(68.0±9.7 vs 59.85±14.1 and 63.4±9.9,respectively,P<0.05).Inverted papillomas were the most common bladder benign tumors;urothelial cell carcinomas were the most common bladder malignant tumors,and adenocarcinomas were the second,squamous cell carcinomas were the third. Conclusion The incidence of bladder malignant carcinomas in our institution increased and the female increased more quickly than male.

Identifying the importance of improving pricing control in military hospitals based on a review of the status quo of such management.In addition to an analysis of difficulties in pricing control in these hospitals,proposing to build an efficient pricing control system with a better understanding of the significance of pricing control.Establishing rules and regulations on medical charges to regulate pricing;making use of computer networks in the management for medical charges transparency;enhancing charges securitization to rule out excessive charges;improving general competence of pricing workers to regulate pricing control,All these five measures will help enhance pricing control in military hospitals.

Objective To investigate the growth inhibition and apoptosis induction effect of vitamin E succinate on MCF-7 human breast cancer cells and to analyze the modulation of Fas expression in this process. (Methods) Estrogen receptor positive MCF-7 human breast cancer cells were treated with VES for 12h, 24h and 48h. The concentrations of VES were 5?g/mL, 10?g/mL and 20?g/mL. The inhibitory effect was measured with MTT method and the cell cycle and cell surface Fas expression were analyzed with flow (cytometry). Fas protein level was detected by Western blotting assay. Results VES had significant inhibitory effect on the growth of MCF-7 human breast cancer cells and the effect was dependently related to time and dosage. The apoptotic rate rose from 1.2% to 11.2%,16.4%, 41.2%,after treated with VES for 48h at the concentrations of 5?g/mL,10?g/mL,20?g/mL respectively. Fas protein level and cell (surface) Fas expression in cancer cells increased after the administration of VES. Conclusions VES had (significant) growth inhibition and apoptosis induction effect on MCF-7 estrogen receptor positive breast cancer cells. The mechanism was related to Fas upregulation on the surface of cancer cells.

At present, the development of hospital in transition period the doctor-patient contradiction, mutu-al trust foundation weak such outstanding contradictions and problems, based on the analysis of facing the outpatient service charge first overall service consciousness; Contradiction link concentration; Hospital information system support does not reach the designated position; Charge personnel are not familiar with services such as business management status, on the basis of train first service consciousness concrete measures are put forward. Namely:strengthen the psychological counseling, advocate humanistic care;carry out training assessment, improve human-istic quality;strengthen the construction of standardization, the standard outpatient service charging process;develop information platform, optimize charging way;strengthen the department cooperation, build a system integration work.

Objective To sum up the experience of nursing patients with spinal tuberculosis combined with open pulmonary tuberculosis? Methods Preoperative nursing was done to 16 patients with spinal tuberculosis combined with open pulmonary tuberculosis to enhance their medical appliance? The careful postoperative observations of conditions and incisions were done to prevent pulmonary infections? Results Of the 16 patients,15 were well cured and 1 was discharged with antituberculotics for home treatment due to refusing to transfer to other hospitals for financial reason? Conclusion The enhanced perioperative health education and nursing is significant for the success of the operations and rehabilitation of the disease?

Objective: To investigate the growth inhibition and apoptosis induction effect of vitamin E succinate (VES) on human colon cancer cells and to analyze the modulation of apoptosis-mediator Fas expression in this process. Methods: Human colon cancer cell line LS174T was treated with VES for 12 h, 24 h and 48 h at the concentrations of 5 mg/L, 10 mg/L and 20mg/L. 1-(4,5-dimethylthiazo-2-yl)-3,5-diphenylformazan (MTT) assay was employed to detect the inhibitory effect of VES on the growth of colon cancer cells. Flow cytometry was then used to analyze the cell cycle of the colon cancer cells after being treated with VES and the apoptotic rate was calculated at the same time. To find out whether the Fas protein expression was modulated in this process, Western blotting assay and flow cytometry were used to detect the Fas protein level in whole cell lystates and on cell surface. Results: VES exhibited a significant inhibitory effect on the growth of human colon cancer cells in a doseand time-dependent manner. After being treated with VES at 5 mg/L, 10 mg/L and 20 mg/L for 48 h, the apoptotic rate of LS174T cells rose from 0.90% to 15.9%, 46.7% and 64.5%, respectively. Fas neutralizing antibody can significantly block VES-induced apoptosis. After the administration of VES, total Fas protein in whole-cell extracts increased in a dose-dependent manner. The flow cytometry showed that the mean fluorescence intensity rose from 5.43 to 9.88, 13.21 and 18.0 after being treated with VES. Conclusion: VES can induce significant growth inhibition and apoptosis in human colon cancer cells. The modulation of Fas expression is one of the mechanisms involved in this process and may be related to the upregulation of Fas molecule on the cancer cell surface.

BACKGROUNDKAI1 is a new identified metastasis-suppressor gene whose expression in many types of tumors has been reported. The aim of study is to investigate the role of KAI1 protein in development of lung cancer and its values in predicting the prognosis of lung cancer.

METHODSThe expressions of KAI1 protein were detected in benign pulmonary disease tissue, precancerous disease tissue, lung cancer tissue and metastatic lung cancer tissue in local lymph node using tissue microarray and immunohistochemical method. The relationship between expression of KAI1 protein and clinicopathological parameters of patients with lung cancer was analyzed by Chi-Square test and Fisher exact test.

RESULTSThe positive rate of KAI1 expression was 100.0% in 10 cases of benign pulmonary diseases, 66.7% in 12 cases of precancerous diseases, 24.7% in 89 cases of primary lung cancer and 0 in metastatic lung cancer tissue in local lymph node respectively. The KAI1 protein expression in primary lung cancer tissues had no remarkable relationship with age and gender of the patients and the location of cancer, but had significant relationship with the histological type and differentiated degree of tumor, P-TNM stages and lymph node metastatic status.

CONCLUSIONSThe abnormal expression of KAI1 protein may participate in malignant progression of lung cancer. Its downregulation may promote the invasion and metastasis of tumor cell. Detection of the expression of KAI1 protein may be helpful to predict the prognosis of lung cancer.