Research on CellSearch system's detection rate of cells and linearity performance detection method, so as to analyze the accurate and reasonable detection method to meet the CellSearch characteristics of the system.
Biomarkers, Tumor ; Cell Line, Tumor ; Cell Separation ; methods ; Humans ; Neoplastic Cells, Circulating

Pachychoroid disease spectrum is a novel concept in ophthalmology upon the development of ocular diagnosis and treatment.Recent breakthroughs and advancements in OCT imaging technology such as enhanceddepth imaging spectral-domain OCT (EDI-OCT) and swept source OCT (SS-OCT) have led to improvements in scanning speed,depth,scanning mode and software.This allows better and more detailed visualization of more posterior and deeper structures such as choroid or sclera.The newly developed EDI-OCT and SS-OCT provide a new basis for the diagnosis and classification of the retinal choroidal diseases.Pachychoroid disease spectrum,a group of chronic persistent choroidal thickening associated with a similar clinical phenotype and pathogenesis of disease were named recently based on higher spatial resolution and more detailed choroidal imaging captured by EDI-OCT or SS-OCT.Ophthalmologists should pay more attentions to this new concept and its relative information.In this paper,the concept,pathogenesis,and phenotype of pachychoroid disease spectrum are emphasized.

In terms of performance of piezoelectric micro pump, this paper explores the piezoelectric ceramic plate in different wave driven micro pump flow rate. The conclusion is that the square wave voltage gets the biggest micro pump velocity. The velocity and pressure of the micro pump is almost linear relationship, and having nothing to do with the different inner diameter pipes. The piezoelectric micro pump's stability is not good and exists attenuation.
Ceramics ; Insulin ; administration & dosage ; Insulin Infusion Systems

Objective To test the hypothesis that the macular pigment may be a marker of foveal cone function and consequently the structural integrity of foveal cones. Methods Sixteen patients (32 eyes) diagnosed to have Stargardt dystrophy and three patients with full thickness macular holes by clinical criteria were studied with a scanning laser ophthalmoscopy (SLO) comparing argon laser blue and infra-red images for the presence or absence of macular pigment (MP) in the fovea. An C ++ computer based program was used to evaluate the density of MP. Eyes were graded into three categories: those without foveal macular pigment, those with partial pigment and those with normal amounts of macular pigment. These categories were compared with visual acuity determined by the Snellen chart. Results Thirteen eyes with a visual acuity of 20/200 or worse had no macular pigment in the fovea. Eleven eyes with visual acuity of 20/40 or better had a normal amount of macular pigment in the fovea and 1 eye had partial macular pigment. Eleven eyes with partial macular pigment had intermediary acuity value. Conclusions Foveal macular pigment is closely related to foveal cone acuity and therefore may be a marker for the presence of foveal cones. Infrared light is a sensitive indicator of early macular diseases

Four macro SAS instructions of covariance analysis are worked out, and a statistical table of covariance analysis is produced with the operation of macro SAS in this paper.

This study was aimed to establish an HPLC method for the determination of liquiritin, isoliquiritin, liquiritigenin and glycyrrhizic acid in roots and knotty rhizome of Glycyrrhiza uralensis. The analysis was performed on a Diamonsil C18 column (250 mm í 4.6 mm, 5 μm) by using a gradient elution with mobile phase of water, phosphoric acid, acetonitrile at the flow rate of 1.0 mL·min-1. The detection wavelength was 276 nm (0~18 min), 360 nm (18~24 min), 276 nm(24~30 min), and 250 nm (30~65 min). The column temperature was set at 30℃. The results showed that the linear range of iquiritin, isoliquiritin, liquiritigenin, glycyrrhizic acid was 0 . 108 5~1 . 085、0 . 016 8~0 . 168、0 . 0049 4~0 . 049 4、0 . 407~4 . 07μg , respectively . The average recoveries of four constituents were 96.61%~100.89%, with RSD ≤ 0.81%. The contents of four constituents in roots of five batches were 0.513%, 0.072 9%, 0.048 4%, and 1.945%, respectively. Contents of four constituents in knotty rhizome from two batches were 0.456%, 0.063 6%, 0.036 2%, and 1.630%, respectively. It was concluded that there was good linear relationship between the response and concentration. Contents of four constituents in knotty rhizome were basically the same as those in the roots. The knotty rhizome can be used as raw material for the extraction of active components.

Objective To establish an optimum DNA extraction method for Chinese traditional medical herbs in order to meet necessary for DNA barcoding research.Methods Four Chinese traditional herbs, Glycyrrhiza uralensis Fisch, Phellodendron Chinensis Cortex, Cistanche tubulosa Wight and Cistanche deserticola Ma were chosen as the experimental materials, the DNA was extracted by 6 different kinds of DNA extraction method, including the improved method of high-salt combined low-pH,the improved method of SDS,CTAB method,PVP method,PlantZol Kit and Ezup Kit, the quality of DNA was investigated by ultraviolet spectrophotometry,agarose gel electrophoresis and PCR amplification by using specific primers of ITS2 and psbA-trnH. ResuIts The quality of the DNA was better than other four kinds of methods by the improved method of high-salt combined low-pH and Ezup Kit, the value of OD260/OD280 was between 1.7 ~1.9,the yield of DNA was the highest by the PlantZol kit , followed by the improved method of high-salt combined low-pH( P <0.05 ) , but the purity of DNA was poor by the PlantZol kit.The DNA electrophoresis tests showed that the DNA integrity of Glycyrrhiza uralensis Fisch and Cistanche tubulosa Wight were better with the improved method of high-salt combined low-pH, the improved SDS method, the CTAB method and the PlantZol kit.The DNA of Phellodendron Chinensis Cortex and Cistanche deserticola Ma were extracted by the six methods appeared diffuse status in the lanes.But only the improved method of high-salt combined low-pH could make the PCR amplification of the success rate 100% by using specific primers of ITS2 and trnH-psbA.ConcIusion The DNA extraction method of high-salt combined low-pH can be used to establish the Chinese DNA barcoding which has the advantages of lower cost, simpler procedure and less time.

Objective:To establish a quality control method for Shuangshengen capsules ( Salvia miltiorrhiza, Panax ginseng and Radix pueraria) . Methods:TLC was performed to identify Salvia miltiorrhiza, Panax ginseng and Radix pueraria. HPLC was used to determine the contents of puerarin and ginsenoside Rg1 . Results:The spots obtained from the test solutions had the same color as the reference solution at the same position. The spots on the TLC plates were clear without any interference. The calibration curve was line-ar within the range of 14. 42-115. 36 ng for puerarin, and the average recovery was 99. 94%(RSD=0. 13%, n=5). The calibration curve was linear within the range of 0. 1-0. 8 μg for ginsenoside Rg1, and the average recovery was 99. 85%(RSD=0. 42%,n=5). Conclusion:The method is reliable, simple and reproducible, and suitable for the quality control of Shuangshengen capsules.

Objective:To summarize the research advance in antitumor activity of dandelion in recent 5 years.Methods:The lit-eratures on the antitumor activity of various extracts of dandelion in recent 5 years were collected , including the effective components such as polysaccharide , triterpenes , phytosterols , flavonoids and organic acids etc .Results:Dandelion showed antitumor activity .The polysaccharide ingredients could effectively prevent the proliferation of pancreatic cancer PANC -1 cells, lupeol could reduce the activity of breast cancer MCF-7 cells, and beta sitosterol could induce the apoptosis of human cervical cancer Hela cells .Conclusion:Dande-lion is effective in the treatment of pancreatic cancer , breast cancer and cervical cance etc .The antitumor activity of the other active in-gredients in dandelion needs further research in order to provide complete theoretical and experimental evidence for the potential appli -cation as antitumor medicine in clinics .

Event-related potentials (ERP) is some kind of brain potentials that recorded from cranial surface when human cognitive processing an object(pay attention, memorize, thinking, etc). ERP consists of a variety of cognitive function and related components. Including early composition, mismatch negativity (MMN), N2 family, P3 family, and other related components of language processing. These components are closely related to human cognitive function,the research of ERP can lead to a deeper understanding of cognitive function of human brains and has important application value of forensic medicine.