Intracranial stent implantation induces platelet-monocyte aggregate, regulates activities of monocyte, induces monocyte expression tissue factors, and promotes fibrin deposition in thrombus at vessel injury site, which promotes inflammatory reaction. P substance significantly increases following stent implantation; platelet ? particle-derived soluble CD 40L, which can promote platelet aggregation, also increases after stenting. Flow cytometry, platelet aggregation, soluble platelet activation-related molecule in serum are used to determine platelet activation and aggregation in blood of 30 patients before and after stent implantation from Department of Neurosurgery, First Hospital of China Medical University, so as to explore blood coagulation reaction characters after intracranial stent implantation

0.05).There was no significant variations of the parameters of newborns according to different levels of maternal serum ferritin. It has been shown that the iron status of mother does not affect the iron status of the infant. Fetus can draw enough iron to satisfy its need for growth and development, and this may cause iron deficiency in the mother. So, prophylaxis of iron deficiency during pregnancy is indicated.

The therapeutic trial of iron on 114 preschool children was studied. Before and after the trial, the iron status of the children was determined by measuring hemoglobin, free erythrocyte protoporphyrin, the ratio of free erythrocyte protoporphyrin/hemoglobin, serum iron, transferrin, transferria saturation, and serum ferritin. All of the children were given a two-month course of ferrous sulfate (4mg iron/kg/ day orally). The iron status of 4 years age group was superior to the 1-4 years group before the therapeutic trial. But after the trial, the iron status of these two groups was very significantly improved, all of the parameters of each group reached at the same levels. Those children with mean value of hemoglobin 3?g/g had good response. Of the 114 children 70 were iron deficient (61.4%), 83 had an increase in venous hemoglobin greater than 0.5g/dl after the trial (72.8%). Since the therapeutic trial is more economical and simpler so we favor to give the trial among the preschool children whose hemoglobin is below 12g/dl and/ or free erythrocyte protophyrin/hemoglobin ratio greater than 3?g/g, in order to avoid missing the iron-responsive individuals.

3,= 4, the sensitivity and specificity in predicting response before iron administration were 77.4, 47.5; 71.7, 75.4; 52.8, 90.2; 18.9, 96.7 respectively. Both sensitivity and specificity were higher in two parameters combined, so that the two parameters combined to screen iron deficiency would be better. Because both sensitivity and specificity were higher in Hb and FEP/Hb, so we prefer Hb and FEP measurement for screening iron deficiency.

0.05). All of the indexes in control group were lower on post-operative day 7 than those of pre-operation. The levels of pre-albumin,transferrin, fibronectin, IgG,IgM, CD~+_3, CD~+_4 T cells and nitrogen balance in the study group were significantly higher than those of control group.Negative nitrogen balance began to be positive in the study group on the post-operative day 7. However, the nitrogen balance in the control group was still negative on the post-operative day 10. The duration of hospitalization in the r-hGH group was shorter than that of the control group. The 1-year, 2-year and 3-year survival rates and recurrence rate were not different between two groups.(Conclusions) The post-operative short-term use of the r-hGH plus HPN in patients with excisable gastrointestinal cancer is safe, effective and benefit for the post-operative recovery.

OBJECTIVE:To discuss the pathways to enhance core competitiveness by the implementation of the Good Supply Practice(GSP)management in Medicine Chain Drug Stores.METHODS:To analysis from sequentiae several aspects: consummate the quality system,create the brand value and conversion the management philosophy in drug retail trade.RESULTS & CONCLUSIONS:Through the implementation of the GSP to create efficiency with management,create brands with quality and offer best after-sale service and customer service so as to enhance enterprises' core competitiveness.

Objective: To construct a 5/11 chimeric adenovirus harboring RGD-4C,and observe its transfection efficiency after transfecting it into hepatocarcinoma cell lines HepG2, BEL-7404 and BJ cells. Methods: RGD-4C was inserted into the HI loop region of 5/11 chimeric adenovirus fiber gene and the insertion outcome was confirmed by enzyme digestion and PCR analysis. The confirmed plasmid was co-transfected into E. coli BJ5183 together with adenoviral backbone plasmid pPE3 to produce recombinant plasmid by homologous recombination. Recombinants were selected and co-transfected into 293 cell line with PDC328-EF1-EGFP to produce recombinant adenovirus. The recombinant adenovirus production was confirmed by PCR analysis and was amplified and purified. The virus titer of recombinant adenovirus was determined. AD11-RGD-4C-EGFP was used to infect HepG2, BEL-7404, and BJ cell lines, and their transduction efficiency was determined by fluorescence microscope. Results: A 1 123 bp target gene fragments was obtained by PCR from the recombinant adenovirus; meanwhile, we prepared high titer recombinant adenovirus, with the titer being 1 3X10 10pfu/ml after purification. At 10 MOI, the infection efficiency of recombinant adenovirus was much higher than control adenovirus(AD11-EGFP) after 48 h infection. Conclusion: We have successfully constructed a chimeric adenovirus harboring RGD-4C, which paves a way for enhancing the infection efficiency of adenovirus in bio-therapy.

Objective To investigate the antitumor activity of the oncolytic adenovirus expressing lipocalin 2 gene for colorectal cancer in vivo.Methods BALB/C nude mice subcutaneously inoculated by SW620 cells and grown tumors were treated with injection of ZD-55 virus, Ad-lipocalin 2 virus and ZD55- lipocalin 2 virus respectively.The weight of implanted tumors and the tumor inhibition rate were calculated to evaluate the anti-tumor effect.Cell apoptosis was determined by TUNEL and the protein expression of VEGF and MVD were determined with immunohistochemistry.Results ZD55-lipocalin 2 inhibited the growth of transplanted tumor more significantly than ZD-55 virus and Ad-lipocalin 2 virus ( P ＜ 0.05 ).Tumor cell apoptosis was upregulated and the MVD reduced significantly in ZD55-lipocalin 2 group in contrast to the other two groups (P ＜0.05).Conclusions ZD55-lipocalin 2 induces apoptosis of colorectal tumor cells and inhibits tumor microvascular formation, slowing down the growth of transplantation tumors.