Objective To explore the significance of IL-17 and IL-10 in patients with different types of hepatic cystic echinococcosis ( CE ) in cyst stages , to investigate the values of evaluation of hydatid cyst activity . Methods 33 patients with hepatic hydatid disease and 21 healthy people (control group) were enrolled, divided into 4 groups, according to WHO ultrasonic classification for CE:Control group (n=21), CEⅠ,Ⅱgroup (active, n=14), CEⅢgroup (transitional, n=10), and CEⅣ,Ⅴgroup (inactive, n=9). The serum concentrations of IL-17 and IL-10 were measured by enzyme-linked immunosorbent assay. Results Serum levels of IL-17 in CEⅢgroup were significantly higher than other groups (P<0.05). There were no significant difference in serum IL-17 levels between CEⅠ,Ⅱgroup [(38.57 ± 3.83) pg/mL] and CEⅣ,Ⅴgroup [(40.22 ± 5.15) pg/mL]. Serum levels of IL-10 were significantly higher in CEⅢgroup [(14.65 ± 2.15) pg/mL] than CEⅠ,Ⅱgroup [(11.38 ± 0.71) pg/mL] (P<0.05), while CEⅠ,Ⅱgroup were significantly higher than CEⅣ,Ⅴgroup [(7.60 ± 0.46) pg/mL] (P<0.05). There were no significant difference of IL-17/IL-10 ratio between CEⅠ, Ⅱgroup (3.44 ± 1.04) and CEⅢgroup (4.60 ± 2.40), and there were no significant difference of IL-17/IL-10 ratio between CEⅢgroup and CEⅣ,Ⅴgroup (5.39 ± 1.95). IL-17/IL-10 ratio in CEⅠ, Ⅱgroup was significantly lower than CEⅣ, Ⅴgroup (P<0.05). The serum concentrations of IL-17 has no correlation with IL-10 in different cyst stages. Conclusions The synergy effect of IL-17 and IL-10 may play the certain role to promote the parasite progress. Neither IL-17 nor IL-10 level could be as an independent marker of echinococcal cysts activity, while IL-17/IL-10 ratio reflect the hydatid cyst activity in certain degree, but as a echinococcal cysts activity marker, further evidence should be needed.

Objective To compare the X-ray features between Hirschsprung alied disease (HAD)and Hirschsprung disease ( HD), and analyze the differentiations. Methods From December 2004 to December 2009, nineteen cases of HAD, aged from 30 days to 10 years (median, 14 months), received barium enema examinations in our institution. Other 19 cases with HD, also received barium enema examinations, were chosen randomly. They were aged from 42 days to 8 years ( median, 8 months). The imaging features of HAD and HD were analyzed retrospectively. The incidence rate of colon stenosis,"truncation sign" and spasm notch and R/C ratio (the longest diameter of rectum/colon) were calculated and compared between these two groups. In all these cases, diagnosis was confirmed by postoperative histopathology. The position of barium retained was also evaluated. These parameters of both groups were compared by x2 and Fisher test. Results There was statistical significance in the incidence rate of colon stenosis between HAD (9/19) and HD (18/19) (x2 = 10.364, P ＜0.01). However, there was no statistical significance in "truncation sign" and spasm notch between HAD (4/19 and 3/19, respectively)and HD ( 1/19 and 1/19, respectively) (P ＞0. 05 for both). R/C ratio was 0. 42 ±0. 15 in HAD group and 0.29±0. 12 in HD group, and there was statistical significance between them (t =2.892,P＜0.01). In HAD group, barium retained in distal sigmoid colon in 1 case (1/19), in distal descending colon in 7 cases (7/19), in distal transverse colon in 1 case (1/19), in total colon in 6 cases (6/19); However, in HD group, barium retained in distal rectum in 3 cases (3/19), in distal sigmoid colon in 13 cases ( 13/19), in distal descending colon in 3 cases (3/19). Conclusions There were some differences in the imaging features between HAD and HD though they presented similar clinic experience. HAD cases presented a lower incidence rate of colon stenosis and a higher R/C ratio than HD cases. In HAD cases, the most common site of barium retained is distal descending colon, while in HD cases, it is distal sigmoid colon.

The fermentation of ?-mannanase from an alkaliphilic Bacillus sp. has been studied in 16L tank. The optimum ventilation quantity and agitation speed were 1:1 vvm and 500r/min respectively. The fermentation cycle was 40 h and the highest ?- mannanase activity was 300 u/ml.

Objective To investigate the feasibility and clinical application value of 3D arterial spin labeling (3DASL) technique in diagnosis of ischemic cerebrovascular disease.Methods Experiment 1:Controlgroup (n=30) and brain infarction group (n=20) were established.All patients were routinely examined including 3DASL and dynamic susceptibility contrast (DSC) enhanced series.The analysis of the control group was performed using signal intensity measurement in three paired regions of interest (ROI) which were selected in left hemisphere centrum semiovale and the opposite hemisphere mirror regions respectively.The ROIs of brain infarction group were selected in the lesion areas and their mirror region.The cerebral blood flow (CBF) of each ROI was measured with 3DASL and DSC respectively.The CBF ratio to each ROI and its mirror ROI were calculated.Data were compared using paired-t test.Experiment 2:Acute cerebral infarction (n=30) and transient ischemic attack (TIA) group (n=30) were established.All patients were routinely examined including 3DASL and diffusion weighted imaging (DWI).The positive rate and the area of the lesions were calculated respectively with 3DASL and DWI and compared.Results Experiment 1:Both the control and brain infarction group demonstrated agreement between 3DASL and DSC.The CBF ratios of the control group were 1.02±0.18 and 1.06±0.24 respectively and revealed no statistically significant between that of 3DASL and DSC (P＞0.05).The CBF ratios of brain infarction group were 0.50±0.12 and 0.48±0.18 respectively,and also revealed no statistically significant between two methods (P＞0.05).Experiment 2:The positive rate of acute cerebral infarction were 100％ with both DWI and 3DASL.The areas of the lesion detected by both methods were:SDwI＜S3DASL,n=22; SDWI ≈-S3DASL,n=8; SDWI＞S3DASL,n=0.The positive rate of TIA by DWI was 0％,and 70％ by 3DASL.Conclusion The noninvasive,safe and repeatable 3DASL technique is almost as sensitive as DSC in the measurement of CBF.However,and it is more sensitive than DWI in diagnosis of TIA.Combining 3DASL and DWI will be helpful in early diagnosis of TIA,finding ischemic penumbra and estimating the blood perfusion of ischemic cerebrovascular disease.

Objective To investigate the release feature of dexamethasone sodium phosphate from thermosensitive in situ gels in vitro. Methods Rotation rheometer was used to measure the changes of viscosity with temperature. The membraneless model was applied in assessing corrosion behavior of gel using a thermostatic shaker (50 r/min) at an amplitude of 2.5 cm, taking phosphate buffered solution (pH 7.2) as releasing media. The release behavior was investigated by HPLC on a C18 reverse column DiamonsilTM (250 mm?4.6 mm, 5 ?m). The mobile phase consisted of triethylamine solution-methanol-acetonitrile (38∶28∶34), pumped at 1.0 ml/min, and the detection wavelength was set at 242 nm. Results When the temperature was near to the sol-gel transition temperature, the viscosity rose suddenly. Taking dexamethasone sodium phosphate (2 ml, pH 7.2) as media, the gel dissolution and drug release rate followed the zero order kinetics, and the cumulative gel dissolution (Q1) and cumulative drug release (Q2) equations were Q1=0.8238t (r=0.999, P

Objective To explore the expression of human epidermal growth factor receptor -3( HER3) in human epidermal growth factor receptor-2(HER2)-positive breast cancer and its relationship with the therapeutic effect of trastuzumab and clinical prognosis .Methods Clinicopathological characteristics of 235 HER2-positive breast cancer patients undergoing surgery in Jiangsu Cancer Hospital from Jan .2007 to Jun.2012 were analyzed retrospectively.The expression of HER3 was detected using immunohistochemisty staining .The expression of HER3 and its correlation with the clinicopathological characteristics were analyzed .All patients were followed-up to find out the impact of HER3 on the disease free survival and the therapeutic effect of trastuzumab .Results The positive rate of HER3 in Luminal B ( HER2 +) and HER2-overexpressing breast cancer was 100/135 (74.1%), and 85/100(85%)respectively.The difference had statistical significance (P<0.05).The histolog-ical grading and the lymph node metastasis were significantly different in Luminal B ( HER2+) breast cancer .The tumor volume , histological grading and lymph node metastasis were significantly different in HER 2-overexpressing breast cancer .The 5-year disease free survival of HER 2-positive breast cancer patients with negative HER 3 was higher than that with positive HER3.The non-relapse survival time was not significantly different between the pos-itive and negative HER 3 expression in Luminal B ( HER2+) breast cancer patients receiving trastuzumab treat-ment , but was significantly different in HER 2-overexpressing breast cancer patients .Conclusions HER3 is cor-related with unfavourable prognosis in HER 2-positive breast cancer .The treatment targeting HER3 may improve the clinical prognosis of both HER 2-positive and HER3-positive breast cancer patients .The HER2-overexpressing breast cancer patients with negative HER 3 may benefit more from trastuzumab treatment .

ObjectiveTo develop a quantitative method for simultaneously determining multi-components in Rhei Radix et Rhizoma using one chemical reference substance.MethodsThe contents of multi-components were calculated by the UV relative correction factors (RCFs) of chrysophanol,physcion,and rhein to emodin.ResultsThe values of RCFs at 274 nm for rhein,chrysophanol,and physcion to emodin were 0.712,0.674,and 1.051.The calibration curves were linear over the ranges of 0.02-4.08,0.02-4.12,0.07- 12.92,and 0.02-3.68 μg/mL for rhein,emodin,chrysophanol,and physcion,respectively.The contents of emodin in 18 samples were determined by the extemal standard method,and the contents of the other three anthraquinone aglycones were calculated according to their RCFs.ConclusionNo significant difference is found in comparison with the classical method,indicating that the RCFs have high reliability within their linear ranges and could be used in quality control of Rhei Radix et Rhizoma.The quantitative analysis of multi-component with a single marker is especially suitable for herbal medicines containing unstable or hard to be purified components as quality control markers.

Objective:This study aims to investigate the correlation between the number of resected lymph nodes (LNs) and the prognosis of patients with node-negative non-small cell lung cancer (NSCLC). Methods:A retrospective review of 305 patients with NSCLC, who received curative resection between January 2004 and December 2009, was conducted. All patients were proved without lymph node involvement histopathologically. The prognostic impact of the number of negative LNs and the clinicopathologic factors were analyzed. Results:The overall median survival time and the 1-, 3-, and 5-year overall survival rates were 60.0 months, 76.1%, 59.3%, and 47.1%, respectively. Survival analysis confirmed that the number of negative LNs, T staging and the stations of the mediasti-nal lymph nodes dissected were showed to be independent prognostic factors. Patients with a high number of negative LNs had better overall survival than patients with a low number of negative LNs (P<0.05). The patients with dissected LNs counts of more than 11 for cases with pTl~2 tumor (P<0.05) and more than 16 for cases with pT3 tumor (P<0.05) had better long-term survival outcomes. The post-operative complication rate was 12.1%, which was not significantly correlated with the number of dissected lymph nodes (P>0.05). Conclusion:The number of negative lymph nodes is an independent prognostic predicting factor for node-negative NSCLC. Sufficient dissection of LNs is recommended to improve the survival of the patients with node-negative NSCLC.

Objective To investigate the correlation between the number of resected lymph nodes(LNs) and the prognosis of patients with node-negative esophageal carcinoma.Methods A retrospective review of 429 patients receiving esophagectomy with morden two-field lymphadenectomy for cancer between January 1998 and December 2008 was performed.All patients were proved without lymph node involvement histopathologically.The prognostic impact of the number of negative LNs and the clinicopathologic factors were analyzed.Results The overall median survival time was 63.0 months,and the 1-,3-,and 5-year overall survival rates were 78.5％,64.0％ and 51.2％,respectively.Survival analysis confirmed that the number of negative LNs and the depth of tumor invasion were showed to be independent prognostic factors.Patients with a high number of negative LNs had better overall survival than patients with a low number of negative LNs(P ＜ 0.05).The patients had better long-term survival outcomes with dissected LNs counts of more than 15 for cases with pT1 tumor(P ＜ 0.05),and more than 20 for cases with pT2-3 tumor(P ＜ 0.05).Conclusion The number of negative lymph nodes is an independent prognostic predicting factor for node-negative esophageal carcinoma.Sufficient dissection of LNs is recommended to improve the survival of patients with node-negative esophageal carcinoma.

Objective To explore the application of tissue-explant technique in culturing rat submandibular gland cell(RSGC)and the characteristics of RSGC.Methods RSGCs were cultured using tissue-explant technique.The cells were purified by enzymatic digestion and differential adhesion.The cell phenotype was identified by cytokeratin-8 immunohistochemical staining.Cellular morphology was observed and photographed under inverted microscope.The cell viability and growth were determined by a double-staining procedure using FDA-PI and MTT assay,respectively.Results Cytokeratin-8 was positive stained in the immunohistochemical staining.The cell viability was more than 95%.The cell growth curve showed that RSGCs were in logarithmic phase since day 5.Conclusion Tissue-explant technique is an easy way to purify plentiful RSGC with normal functions,and it can be used in further research of tissue engineering of submandibular gland.