Objective To investigate the antitumor activity of the filtrate of a novel Staphylococcus aureus with incompletehemolytic phenotype(SIHP), and to provide references for screening new antitumor drugs.MethodsThe epidemiologicalcharacteristics of the novel SIHP were summarized and analyzed. The hemolytic phenotypes of the novel SIHP, classic Staphy-lococcus aureus with complete hemolytic phenotype(SCHP) and quality control strains ATCC29213 and ATCC25923 weredetected on blood agar plate(BAP) by three-point method. Staphylococcus aureus filtrate was prepared by inoculating LBbroth, centrifuging and filtering after fermentation. The Staphylococcus aureus filtrate and its diluents with RPMI1640 ofdifferent volume fractions(1∶1, 1∶3, 1∶7) were co-incubated with human non-small cell lung cancer HCC827 cells, themorphological changes were observed, and the cells were classified and counted under a microscope.ResultsThe clinicalspecimen isolation rate of the novel SIHP was only 2. 01%, and its hemolytic phenotype on BAP was unique with strongrecognition. Compared with the classical SCHP and quality control strain ATCC25923, the novel SIHP filtrate showed signifi-cantly stronger tumor cytotoxicity, and damaged HCC827 cells in a concentration-dependent manner, leading to the release of cell contents, balloon-like degeneration of cell membrane or bare nucleus-like degeneration of cells.ConclusionThe novel SIHP may be a new subspecies of Staphylococcus aureus with a unique genetic background, and the filtrate contains highly effective antitumor active substances, which can provide a material basis for the screening of new antitumor drugs.

Objective To study CT and MRI value in diagnosis of osteoblastoma,in order to achive more information about the disease. Methods The clinical,CT,MRI and histological data of 12 patients with osteoblastomas. Results(1)There were 6 patients wereexamined by CT,the lesions showed mainly lytic with varying degrees of matrix mineralisation,and mild adjacent reactive sclerosis;(2)10 patients were examined by MRI(included 4 patients who have been examined by CT),the lesions presented mixed signal intensity with compart,slightly low and intermediate signal intensity on T_1-weighted images,high signal intensity on T_2-weighted images were found in 9 cases and mainly low signal intensity was found in 1 case.8 cases showed clear low signal intensity surrounding the lension borh on T_1 and T_2,2 cases showed blurry high signal in the adjacent tissue;(3) The tumors presented moderate enhancemen with low dense area inside the lesions after injection of contrast media. Conclusion Osteoblastomas have classical features on CT,while show no characteristic appearances on MRI.

The clinical manifestation of endometrial uterine cancers is similar to other gynecological diseases like endometrial hyperplasia,polyps,uterine myoma,and adenomyosis.This similarity may result in missed diagnosis of endometrial cancer,which should be paid wide attention.In these cases,pathological examination reports malignancy of uterine specimen after simple hysterectomy.Personalized treatment,such as observation and reoperation,should be supplemented for these patients,according to the pathological characteristics of the tumor,risk factors of lymphatic and ovarian metastasis and the willingness of patient.

Objective To study the conventional MRI features of adult Japanese encephalitis (JE)and explore the early features of diffusion weighted imaging(DWI).Methods Thirty-two patients with adult JE were included.MR scanning including T1 WI,T2 WI and FLAIR were done in all patients,and DWI in 28 patients.Twenty-three patients received DWI in the early stage (within 1 to 7 days),in which the appearances of DWI were analyzed,ADC graphs were obtained and the signal noise ratio and the ADC values of lesions were calculated.Results Twenty-eight cases were abnormal on MRI,and 4 patients were normal.The lesions of JE involved the thalamus in 22 cases,the substantia nigra of midbrain in 13,kellogg’s seahorse in 9,the basal ganglia in 7,the splenium corpo-ris callosi in 4,the pons in 6 and the insular lobe in 2,extensive cerebral edema in 2.The early features of DWI in 23 patients showed cytotoxic edema in 2,vasogenic edema in 12,both two edemas existing in 5,and normal in 3.DWI were superior to T2 WI in 6 cases,and T2 WI were superior to DWI in 9 cases,DWI was similar to T2 WI in 5 cases on showing the size and signal of the le-sions.Conclusion Adult JE often occurs in the thalamus,substantia nigra of midbrain and kellogg’s seahorse.DWI shows vasogenic edema in the early stage.Comprehensive analysis of DWI,ADC and T2 WI details helps to early detection and qualitative adult JE le-sions.

Objective: To demonstrate that expression of the CCRSDelta32 protein in PBMCs able to down-regulate surface expression of the HIV-1 coreceptor CCR5 and CXCR4.Methods:CCR5Delta32 gene was amplified from human peripheral blood mononuclear cells(PBMCs)genomic DNA by using PCR, and then cloned into lentiviral vector pLenti6/V5-D-TOPO.Recombinant lentiviral particles were produced by packaging using 293T cells.Human PBMCs were transfected with the constructed recombinant lentiviral particles and the expression of CCR5Delta32 was detected by Western blot.The level of CCR5 and CXCR4 expression on transfected PBMCs was detected by FACS analysis.Results: The recombinant lentiviral vector pLenti-CCR5Delta32 was constructed successfully, and the target protein was expressed in PBMCs.FACS analysis showed that CCR5Delta32 protein expressed in PBMCs was able to down-regulate cell surface expression of CCR5 and CXCR4.Conclusion: This study is expected to be used for the gene therapy on AIDS, which deserves further study.

Background and purpose: The incidence of Hashimoto's disease (HD) coexistent with thyroid carcinoma is 0.5%-38%, and it is increased significantly in recent years. Therefore, we investigated the clinical characteristics and surgical experiences in patients with HD coexistent with thyroid neoplasm. Methods: The clinical data in 417 cases of HD coexistent with thyroid nodules including 93 cases of HD coexistent with thyroid carcinoma treated surgically and confirmed pathologically from Jun. 1985 to Dec. 2007 were analyzed. Results: The coexistent rate of HD with thyroid carcinoma (TC) was 22.3% respectively. The coexistent rate of HD with TC was 9.2% before 1995, and it went up to 24.7% after that year (P<0.05). Among those HD coexistent with TC patients, 66 cases were papillary carcinoma, 15 cases were follicular carcinoma, 9 cases were mixture and 3 cases were lymphoma. There were 43 cases of micro-foci thyroid carcinoma, which accounted for 46.2% of the malignancy. Unilateral or bilateral, total, subtotal or partial thyroidectomy and biopsy were the main operative procedures for the disease. Conclusion: There is a high incidence of thyroid carcinoma in patients with HD coexistent with thyroid nodules, and it is rising up recently. In the clinical treatment of HD, we should be aware of the coexistent thyroid tumors and micro-foci thyroid carcinoma.

Background and purpose:The incidence of Hashimoto’s disease (HD) coexistent with thyroid carcinoma is 0.5%-38%, and it is increased significantly in recent years. Therefore, we investigated the clinical characteristics and surgical experiences in patients with HD coexistent with thyroid neoplasm. Methods:The clinical data in 417 cases of HD coexistent with thyroid nodules including 93 cases of HD coexistent with thyroid carcinoma treated surgically and confirmed pathologically from Jun. 1985 to Dec. 2007 were analyzed. Results:The coexistent rate of HD with thyroid carcinoma (TC) was 22.3% respectively. The coexistent rate of HD with TC was 9.2% before 1995, and it went up to 24.7% after that year (P

Objective To investigate the characteristics of brain imaging and its relationship with the cognitive disorders in vascular dementia patients. Methods The volumes of cerebral lobes and hippocampus formation in 30 ischemic vascular dementia patients and 30 normal controls were measured with MRI based technique. The indices of ventricles and cerebral sulci in 30 ischemic vascular dementia patients and 30 patients with ischemic stroke were calculated with CT based technique. The cognitive functions were assessed by psychometric testing (Mini Mental State Examination MMSE, Brief Screening Scale for Dementia BSSD, Raven's Standard Progress Matric RSPM) in all subjects. Results The volumes of frontal and temporal lobes in vascular dementia patients were smaller than those in ischemic stroke patients and normal control subjects ( P 0.05). The decrease of the volumes of the frontal and temporal lobes were positively correlated with the decrease of MMSE and BSSD scores. The cognitive disorders were more severe in the ischemic vascular dementia patients with pathological foci in the frontal and temporal lobes, multiple infarcts, and the total volume of infarcts greater than 50 mm 3 ( P

The literature retrieval course is the main form of current domestic medical infor-mation literacy education. But students have no interest in it, so it's difficult to realize the goal of in-formation literacy education. Information literacy education has the same ideas and goals with PBL in developing students' ability to ask questions, analyze and solve problems, as well as in improving students' autonomous learning and life-long learning ability. To study the model of medical information literacy education embedded in PBL from three stages that early course preparation, mid implementa-tion and later evaluation. The research is aimed at the improvement of students' information literacy and their lifelong study ability. It suggests that there are coordinated effects between PBL and informa-tion literacy.

Diffuse large B-cell lymphoma (DLBCL) is the most common type of non-Hodgkin lymphoma with highly heterogeneous clinical course. How to recognize patients with high risk is coming into the focus of the 57th American Society of Hematology (ASH) annual meeting. The International Prognostic Index (IPI) score as a classic prognostic system is challenged by variety of new prognosis systems. The tumor microenvironment plays an important role in progression of lymphoma, and its prognostic value draw much more attention. The value of PET-CT for prognosis of DLBCL has also been affirmed in this meeting.