Septic shock induced by gram negative bacteria is the leading cause of inpatient mortality. Now there are two ways in endotoxin induced cellular response: the indirect way of cytokines and the direct way. The article reviewed mechanisms of the direct intracellular way induced by endotoxin.Paid special attentions to the development of transmembrane, intracellular and intranuclear signalling and looked ahead the application in clinic.

As one of pharmaconutrients,it has been proved that ?-3 polyunsaturated fatty acids can regulate inflammation and immunological function because not only its metabolites are different from other lipids but also it can affect intracellular signal transduction.So the structure、composition and molecular mechanisms of ?-3 polyunsaturated fatty acids are reviewed in this article.

As one of lipid nutrients,it has been proved that ?-3 polyunsaturaed fatty acids can effectively be used in the patients with sepsis,cancer,transplantation,chronic inflammatory diseases and so on,which is related with the molecular mechanisms of regulating the structure and function,cytokines and so on.So the clinical study of ?-3 polyunsaturaed fatty acids on different diseases is reviewed in this article.

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Laterally spreading tumor is a new type of colorectal tumor found recently.For its particular shape and develptment of tumor,LST is divided as an independent tumor.LST is associated with colorectal cancer and more research is done on it .The progress of its epidemiology, oncomolecularbiology, diagnosis and therapy is reviewed.

Aim To investigate roles of ROS in oxaliplatin-induced PUMA expression and apoptosis in colon cancer cells.Methods ROS was used as an oxidative stress in vitro and PUMA expression was determined by Western blot analysis,the apoptosis induced by ROS was assessed by Hoechst 33258 dye staining,the proliferation of the colon cancer cells treated with oxaliplatin and antioxidant was determined by MTT assay.Results ROS induced apoptosis and PUMA expression in colon cancer cells;suppression of PUMA expression by stable transfecting PUMA anti-sense vector decreased apoptosis induced by ROS;oxaliplatin-induced PUMA expression was abrogated by antioxidant and the proliferation of colon cancer cells treated with oxalipla-tin was increased by antioxidant.Conclusions Our data suggests that PUMA and ROS play important roles in oxaliplatin-induced apoptosis.Oxaliplatin induces PUMA expression and apoptosis partly through ROS in colon cancer cells.

Objective To clone and analyze rat albumin gene. Methods Total RNA was prepared from rat hepatocytes. cDNA fragment encoding rat albumin was amplified by RT-PCR, and then was cloned into pGEM-T vector. Inserted rat gene was sequenced by ABI PRISM 377DNA Sequencer. Meanwhile, the albumin mRNA expression was observed in hepatocytes treated with 1ng/ml and 1g/ml LPS. Results Rat albumin gene was successfully cloned. The expression of albumin mRNA was inhibited by LPS, and the inhibition was dose dependent. Conclusion The cloned rat albumin gene was composed of 401 base pairs and had 100% nucleotide homology with that reported in Genbank. The albumin expression was decreased in hepatocytes treated with LPS. A stable method of semi-quantitative analysis of rat albumin mRNA was established.

Objective To investigate the effects of different concentrations of glutnine(Gln) on the procfion of nitric oxide (NO)and reduction in mitochondrial membrane potential in rat heputocytesactivated in by interleukin(IL)-1β.Method The primary cultured rat hepatocytes with high-purity Wfffe isolated from 6-week-old male Sprague-Dawley raL, by flirt in situ circulatory collagenase peffusion method.After incubation for hours.hepatocytes were stimulated by saline,or IL-l(1 nmol/L)or IL-I(1 mnol/L)combined with gIne in concentrations rangiIfrom 2 to 10 mmol/L.The culture muln and hepatocytes were collected at 24 hours after stimulation.The concentrations of alanine aminotransferase(ALT)and NO in the lnllln were detected by biochemical methods.The mitochondrial membrane potential of the hepatocytes was detected with flow cytometry after incubated with fluorescem probe JC-1.Statistic package ofSPSS 11.5 was used for the data analysand significant differences between mean8 were evaluated byQ、4k analysis.Results The average concentration ofand NOinthe culturemedium afterIL-Istimulationwas 38.2U/L and 72.7tmaol/L,respectively,whichwere sis,cantly big,herthanthose ofcontrol group(7.4 U/L and41.7nol/L,respectively,P<0.01).1hemitochondrial membrane potential ofhepatocytes in IL-lgroup was much lowerthan that in control group(30.O%vs.62.8%.P<0.01).Gin inhibited NO production induced byⅡ,lp,Jeleasing and reduction in mitochondrial II brahetential ofcuhured rat hepatocytes in a dose-dependent HmIll.Conclusions Ghtamine,the most abundant free amino acid in the body,call attenuate hepatocye injury mechateel in vitro by pro-inflammatory cytokine mediated.nlis protective effectmay be associatedwiththeinhibition of NO production and thereby amelioration of mitochondrialfunction.

Objective: To identify variables that significantly predict the intention of NaFeEDTA fortified soy sauce (FeSS) consumption and to develop feasible intervention strategies.Methods: Stratified sampling and random sampling were adopted together.636 women from rural areas and 454 from urban areas completed eligible questionnaires.Theory of Planned Behavior (TPB) and Health Belief Model (HBM) were adopted to design the constructive questionnaire.Path analysis was used to construct the model of intentions to buy FeSS.Results: The model explained 35%(the rural model, RM)or 55%(the urban model, UM) of the variance of behavioral intention.FeSS knowledge directly and indirectly affected the intention to buy FeSS ( the total effect on the intention, 0.151 in RM, 0.368 in UM).And the behavioral intention was also impacted by women's health value and cognition on perceived susceptibility and perceived severity of iron deficiency anemia(IDA) through the behavior identity(0.176 in RM, 0.354 in UM) and attitudes towards behavior(0.161 in RM, 0.091 in UM).Cues, as an external factor, much affected the intention (0.372 in RM, 0.337 in UM).The external control belief was a rather weak factor affecting intention (0.086 in RM, 0.108 in UM).Conclusion: Integrated TPB and HBM could explain the behavioral intention of FeSS consumption among women in Guizhou.Nutrition education should emphasize behavioral attitudes and identity, and improve knowledge of FeSS and perception of IDA, which may help to remove worries about FeSS.