Objective: To investigate the mechanism that glutamine downregulates the cytokine expression in lipopolysaccharide-stimulated human peripheral blood mononuclear cells.Methods:PMBC were extracted from healthy volunteer by density gradient centrifugation,and stimulated by lipopolysaccharide.The release of TNF-α and IL-10 was analyzed via enzyme-linked immunosorbent assay and p-P38 via western blot. Results:Gln led to an increase in HSP70 expression,and decreased TNF-α、IL-10 release at 4 h after LPS stimulation.The expressions level of TNF-α、IL-10 and P-P38 were decreased after using SB203580.Conclusion:These results indicate that the effect of Gln could attenuate cytokine release in PBMC and might may be related to the inhibition of P38 MAPK.

Objective To investigate the role of glutathione (GSH) synthesis in the regulation of Glutamine (Gin) on TNF-α release in lipopolysaccharide (LPS)-stimulated alveolar epithelial type Ⅱ cells (AEC Ⅱ). Method Primary cultured AECⅡ were divided into six groups, including control, 10.0 mmol/L Gln, 1 μg/mL LPS and LPS+(0.5, 2.0 and 10.0 mmol/L) Gln. In another set of experiments, AECⅡ were divided into six groups including 1 μg/mL LPS, LPS+10.0 mmol/L Gln, LPS+100 μmol/L L-buthionine-(S, R)-sulfoximine (BSO), LPS+Gln+(1,10 and 100 μmol/L) BSO. Each group had three samples. BSO and Gln were added at 8 hours before LPS challenge. After 24 hours of LPS stimulation, cells were obtained for GSH measurement by 5, 5'-dithiobis-(2-nitrobenzoic acid) (DTNB) method. TNF-α level in the supematant was determined by enzyme-hnked immunesorbent assay (ELISA). ANOVA (LSD-t test) was used for statistical analysis. Results Supple-mentation of Gin increased the GSH level and attenuated TNF-α release in LPS-stimulated AEC Ⅱ in a dose-depen-dam manner. GSH level increased from (50.69±3.04) pmol/mg cell (LPS group) to (126.74±7.13) pmol/mg cell (LPS+10.0 mmol/L group) (P<0.01) and TNF-α level decreased from (1104.5±48.8) pg/mL (LPS group) to (329.67±48.27) pg/mL (LPS+10.0 mmol/L group) (P<0.01). BSO, an GSH synthesis blocker, at doses greater than 10 μmol/L reversed the effect of Gin significantly (P<0.01). Conclusions As a precursor ofGSH, glutsmine could attenuate TNT-α release in LPS-stimulated AECⅡ,and the with may be mediated via GSH synthesis.

To observe the protective effects of echinacoside on rotenone-induced damages in rats.

Objective:To prepare the egg yolk immunoglobulin Y ( IgY) against human Sucrase and study its stability,in vitro activity. Methods:Hy-line laying hens were immunized with human Sucrase protein,IgY was isolated and purified from egg yolks of im-munized hens using water dilution and salting out method. Indirect ELISA was used to evaluate the titer and stability of IgY. The purity and specificity of IgY were analysed by SDS-PAGE and Western blot respectively. The inhibitory effects of IgY on α-glucosidase was studied by PNPG method. Results:Indirect ELISA results showed IgY could be detected on the tenth day after the first immunization, and the peak titer of IgY was 1:12 800 after the 40th day of immunization. SDS-PAGE showed that the heavy chain and light chain of IgY were 65 kD and 25 kD respectively, and the IgY against human Sucrase could specifically recognize the protein of human Sucrase. The IgY maintained primary titer when it was kept between 29-69℃ for 15 min,and pH 4-7,37℃,4 h. The titer of IgY was maintained 50% after digestion by pepsin and trypsin respectively for 2 hours. IgY had a higher resistence to pepsin than trypsin after longer digestion time. IgY showed an inhibitory effect on α-glucosidase in concentration dependent manner. The half inhibitory concentration (IC50) was 0. 540 mg/ml. Conclusion:The IgY against human Sucrase has been successfully obtained,which established foundations for its study of Type 2 diabetes mellitus rat models in vivo.

Objective To study the important virulence regulation genes of Brucella,and to understand their function.Methods Quantitative RT-PCR was used to quantify their relative transcription profiles under stress conditions and during macrophage cell infection.Results These genes were activated at different levels under these conditions and during cell infection,indicating their roles in pathogenesis at different srage of infection.Conclusion The transcription profiles of these genes have different effects about their functions.

Poorly differentiated thyroid carcinoma (PDTC) is a special type of thyroid carcinoma, the morphology and biological behavior of which are between well-differentiated and undifferentiated (anaplastic) carcinomas. Currently, the diagnosis of PDTC mainly relies on the pathological standards. Although "Turin standards" is commonly used, there is no generally accepted diagnostic criteria. Surgery is still the main treatment for PDTC, but the adjuvant therapies are in dispute. Age, tumor node metastasis (TNM) stage and integrity of surgical of PDTC are major factors that affect the prognosis. The identification of eosinophilic phenotype (hurthle cells) of PDTC is important. Some common immunohistochemical and molecular biomarkers, such as the insulin-like growth factor II mRNA-binding protein 3 (IMP3), E-cadherin and proliferating protein Ki67, may be helpful for distinguishing PDTC from other thyroid carcinoma. With the progress in studies regarding molecular markers for PDTC and the clinical characters of PDTC patients with large samples, the diagnosis for PDTC will greatly improved and the pathogenesis for PDTC will be elucidated.
Biomarkers, Tumor ; metabolism ; Cadherins ; metabolism ; Carcinoma ; pathology ; Combined Modality Therapy ; Humans ; Insulin-Like Growth Factor II ; metabolism ; Ki-67 Antigen ; metabolism ; Prognosis ; RNA-Binding Proteins ; metabolism ; Thyroid Neoplasms ; pathology

Objective:To explore the effect of discharge planning based on individual and family self-management theory (IFSMT) in preventive enterostomy patients.Methods:From December 2019 to May 2020, convenience sampling was used to select 72 patients who underwent preventive enterostomy in the Gastrointestinal Surgery of the Affiliated Hospital of Qingdao University as the research object. According to the time of admission, patients were divided into intervention group and control group, with 36 cases in each group.Control group implemented routine nursing, and intervention group carried out IFSMT-based discharge planning intervention on the basis of routine nursing. The Enterostomy Patients Self-management Questionnaire and the Chinese version of Readiness for Hospital Discharge Scale (RHDS) were used to evaluate the intervention effect. Finally, 36 patients in intervention group and 35 patients in control group completed the study.Results:Finally, 36 patients in the intervention group and 35 patients in the control group completed the study. After intervention, the differences in the total score and the scores of each dimension of the Enterostomy Patients Self-management Questionnaire between the two groups of patients were statistically significant ( P<0.05) . After intervention, the score of the Chinese version of RHDS of intervention group was (96.61±3.83) , which was higher than that (79.64±5.48) of control group, and the difference was statistically significant ( P<0.01) . Conclusions:The IFSMT-based discharge planning intervention can improve the self-management ability and readiness for discharge of patients with preventive enterostomy.

OBJECTIVETo investigate the effect of fish oil on intestinal Paneth cells in mouse with abdominal infection.

METHODSFifty C57BL/6J mouse were randomly divided into five groups (n=10 each): control group, sham group, infection group (cecal ligation and puncture, CLP), fish oil group (0.4 g/kg fish oil, intragastric administration every day, FO) and long chain triglyceride group (0.4 g/kg soybean oil, intragastric administration every day, LCT). The mouse were sacrificed and the terminal ileum was collected for lysozyme, cryptdin 4 and secreted phosphatidase A2 (sPLA2) analysis at the fourth day after operation. The changes of mouse body weight and intestinal mucosa pathology were observed.

RESULTSThe body weight, the mRNA levels of lysozyme, cryptdin 4 and sPLA2 and the protein level of lysozyme of Paneth cells in the infection group were reduced compared with the control group (0.78±0.34 vs. 1.83±0.11, 0.99±0.44 vs. 2.02±0.33, 0.92±0.25 vs. 1.50±0.27, 0.31±0.06 vs. 0.45±0.05, all P<0.05), meanwhile the intestinal villi collapse and breakage occurred obviously. Fish oil could up-regulate the mRNA and protein expression of lysozyme (1.23±0.27 vs. 0.78±0.34 and 0.62±0.23, 0.38±0.07 vs. 0.31±0.06 and 0.32±0.06, all P<0.05) and alleviate the mucosa injury compared with the infection group and LCT group.

CONCLUSIONSThe function of intestinal Paneth cells is damaged apparently after cecal ligation and puncture. Fish oil can relieve this injury.

Animals ; Cecum ; Fish Oils ; Intestinal Mucosa ; Intestine, Small ; Intraabdominal Infections ; Mice ; Mice, Inbred C57BL ; Paneth Cells ; Up-Regulation

Objective To investigate whether timing of image acquisition influenced infarct size estimation using delayed CeMRI,and the association of left ventricular ejection fraction between magnetic resol3anee imaging and left ventrieulography Was also studied.Method From Junary 2005 to April 2006,27 first,onset AMI patients [23 male,mean age(54.3±10.5)years]were enrolledinthistudr.Allpatients receivedleft ventrictdographyas well as coronary angiography.The average checking time was(13.2±5.2)clays after the onset of AMI.MR imaging was performed with a 1.5-T magnet(SIMENS).After breath-hold eine images were acquired,patients re.ceived afI intravenous bolus of 0.05 mmol/kg Gd-DTPA at a rate of 5 ml/8.A first-pass perfusion scan was ac.qllired.Then a second bolus of 0.15 mmoVkg Gd-DTPA was give.at a rate of 2 mE/Is.After the hyperenhancement localized,the typical short axis slice with hyperenhancement WaS chosen to repeat imaging for IlleasuriIin.farct size every5minutesfrom5minutes after secondinjection ofcontrast until 20minutes.Results Twexty-seren patients showed hyperenhancement at the delayed CeMRI and hypoenhancement at the first pass enhancement(FPE).The average infarct size estimated by CeMRI WaS(17.9士9.8)%of LV nlass.Myocardial enhancement at a repesentative short-axis slice WIllS(7.2±6.2)%of LV Imss at 5 minutes,(8.5±7.4)%at 10 minutes,(7.3±6.3)%at 15 minutes and(6.9-t-6.4)%at 20 minutes respectively.There WltlS significant difference be-tween lmfninmes and 20-minutes enhancement size(P<0.05).Correlations of EF obtained by cineventriculo-grapIIy and MR irr,lg were significant(r=0.867,P<0.01).There were also correlations between infarction size and pe.k CK(r:O.819,P

Objective To study the intrinsic relationships between the binding energy of the antibody light and heavy chains and the conformational characteristics , physical and chemical properties , and to establish a corresponding mathemat-ical model and evaluate the thermal stability of the antibody molecules , which contribute to the antibody design , optimiza-tion and affinity maturation .Methods Based on bioinformatics and computational biology methods , the antibody′s structur-al information with the crystal diffraction data was analyzed .The conformational character of the variable domain of the antibody was studied using distance geometry and computer graphics technology .With the aid of the intermolecular hydrogen bond formation theory and the reaction free energy theory , the dynamic structure and energy characteristics be-tween the heavy and light chain variable regions of the antibody were studied .Furthermore , using nonlinear fitting and regression analysis, a mathematical model was set up .Results According to simulation and statistic analysis , there was a linear relationship between the binding energy and the number of the intermolecular hydrogen bonding , Van der Waals interaction of the heavy and light chains of the antibody .There was polynomial correlation between the binding energy and the physicochemical properties of the antibody .Using the frequency of amino acid position and the established model , the humanized anti-ricin antibody , which could not obtain the stable engineering cell line , was evaluated and optimized .The stable engineering cell line of the humanized anti-ricin antibody was obtained in the experiment .Conclusion The self structure of the antibody variable region ( conformation and physicochemical properties ) has much effect on its stability . The antibody stability can be improved by structural optimization .