Objective To evalute the feasibility of a double hand allogroft to reconstuct the limbs'function. Methods A brain dead donor was chosen under the test of blood group in ABO and Rh,HLA match,PRA and the mixed leukocyte culture.The level of the transplantated hands was 5 cm proximal to the wrists The procedure of the allotransplantation was similar to that of the autologous replantation.The immunosuppressants were taken two days before the operation,in the operation and after the operation. The circulation,the vital signs and the rejection were under the supervision. Results The progress was satisfactory. No rejection was found postoperation.After 10 weeks the callus in both ends of radius and ulna was obvious.After 5 months sensation recovered.After 7 months the internal fixation(plates and screws) was removed and tenolysis was done. Now the recipient could use engrafted hands to wash face,make the bed,wear clothes,brush teeth,peel oranges,eat with a spoon,tie the shoelace slowly,pick up a telephoneor and use the conroller of Television. Conclusions With the immunosuppressive agents,the double hands allograft can survive.The growth rate of bone and nerve is faster than that of autologous replantation.

Objective To investigate the predictive efficiency of combined uterine artery hemo-dynamic parameters and oxidative stress indicators in early pregnancy on hypertensive diseases in pregnancy(HDP).Methods A total of 90 HDP patients(case group)and 30 healthy pregnant women(control group)as research subjects.The case group included 30 patients with pregnancy-in-duced hypertension,30 patients with preeclampsia,and 30 patients with severe preeclampsia,respec-tively,and were divided into pregnancy-induced hypertension group,preeclampsia group,and severe preeclampsia group.Serum levels of reactive oxygen species(ROS),superoxide dismutase(SOD),heme oxygenase-1(HO-1),glutathione peroxidase(GSH-PX),malondialdehyde(MDA),and uterine artery hemodynamic parameters were compared between the two groups in early pregnancy(8 weeks to 13 weeks plus 6 days).The receiver operating characteristic(ROC)curve was used to evaluate the predictive efficiency of relevant indicators for HDP.Results The serum levels of SOD and GSH-PX were lower in the case group than that in the control group,and the serum level of MDA and the uterine artery pulsatility index(PI)were higher in the case group than that in the control group(P＜0.05).The serum levels of SOD and GSH-PX gradually decreased,and the serum levels of MDA and PI gradu-ally increased in the pregnancy-induced hypertension group,the preeclampsia group,and the severe preeclampsia group(P＜0.05).The ROC curve showed that the combination of SOD,GSH-PX,MDA,and PI in early pregnancy had a higher predictive efficiency for HDP,with an area under the curve of 0.824,a sensitivity of 81.1％,and a specificity of 76.7％.Conclusion Patients with HDP have changes in oxidative stress indicators and uterine artery hemodynamic parameters in early preg-nancy,and there are differences among patients with different disease severities.The combined de-tection of SOD,GSH-PX,MDA,and PI in early pregnancy can help predict HDP.

Objective To investigate the predictive efficiency of combined uterine artery hemo-dynamic parameters and oxidative stress indicators in early pregnancy on hypertensive diseases in pregnancy(HDP).Methods A total of 90 HDP patients(case group)and 30 healthy pregnant women(control group)as research subjects.The case group included 30 patients with pregnancy-in-duced hypertension,30 patients with preeclampsia,and 30 patients with severe preeclampsia,respec-tively,and were divided into pregnancy-induced hypertension group,preeclampsia group,and severe preeclampsia group.Serum levels of reactive oxygen species(ROS),superoxide dismutase(SOD),heme oxygenase-1(HO-1),glutathione peroxidase(GSH-PX),malondialdehyde(MDA),and uterine artery hemodynamic parameters were compared between the two groups in early pregnancy(8 weeks to 13 weeks plus 6 days).The receiver operating characteristic(ROC)curve was used to evaluate the predictive efficiency of relevant indicators for HDP.Results The serum levels of SOD and GSH-PX were lower in the case group than that in the control group,and the serum level of MDA and the uterine artery pulsatility index(PI)were higher in the case group than that in the control group(P＜0.05).The serum levels of SOD and GSH-PX gradually decreased,and the serum levels of MDA and PI gradu-ally increased in the pregnancy-induced hypertension group,the preeclampsia group,and the severe preeclampsia group(P＜0.05).The ROC curve showed that the combination of SOD,GSH-PX,MDA,and PI in early pregnancy had a higher predictive efficiency for HDP,with an area under the curve of 0.824,a sensitivity of 81.1％,and a specificity of 76.7％.Conclusion Patients with HDP have changes in oxidative stress indicators and uterine artery hemodynamic parameters in early preg-nancy,and there are differences among patients with different disease severities.The combined de-tection of SOD,GSH-PX,MDA,and PI in early pregnancy can help predict HDP.

Objective:To evaluate the clinical efficacy and adverse events of 192Ir high-dose rate brachytherapy (HDR-BT) in the treatment of locally recurrent non-small cell lung cancer (NSCLC). Methods:Clinical data of 22 cases of recurrent NSCLC after radiotherapy admitted to our hospital from September 2013 to March 2018 were retrospectively analyzed. 192Ir HDR-BT was adopted for reradiotherapy. The prescription dose was 30Gy for 1 fraction. CT scan was reviewed every 1 month in the first 3 months after treatment and every 3 months after 3 months. Local control rate and adverse events were evaluated. The 1-and 2-year overall survival (OS) rates of re-treatment after relapse were calculated. Results:All the 22 patients completed the treatment successfully. The 1-, 3-and 6-month complete response (CR) rates were 9%, 14% and 14%, 82%, 82% and 82% for the partial response (PR) rates, 5%, 0% and 0% for the stable disease (SD) rates, 5%, 5% and 5% for the progressive disease (PD) rates, 91%, 96% and 96% for the objective response rates (ORR), respectively. The 1-and 2-year OS rates of re-treatment after relapse were 59% and 27%. Five patients (23%) experienced acute radiation-induced pneumonitis (3 cases of grade 1 and 2 cases of grade Ⅱ), 4 cases (18%) of radiation-induced bone marrow suppression (3 cases of grade I leukopenia and 1 case of grade I thrombocytopenia) and 1 case of postoperative pneumothorax. All these adverse events were mitigated after symptomatic treatment.Conclusion:192Ir HDR-BT is an efficacious and safe treatment of locally recurrent NSCLC.

Objective: To optimize the membrane filtration method for hepatitis A virus concentration from mimicked water samples. Methods: Mimicked water samples containing HAV particles were prepared and concentrated by positively charged membrane and negatively charged membrane respectively. Then different method including direct lysis, shaker, vortex and ultrasonication were used to elute HAV followed by the quantification of HAV by Taqman Real-time RT-PCR. The data were analyzed by professional statistical software. Results: In the present study, when mimicked water samples contained 300 TCID₅₀ of HAV, there was no significant difference between the concentration effects by negatively charged membrane and positively charged membrane (P=0.825>0.05). However, when HAV in mimic water samples was up to 1500 TCID₅₀ and 7500 TCID₅₀, the recycle efficiency by negatively charged membrane was higher than that by positively charged membrane (P<0.01). Additionally, this study found that HAV recycle ratio could be up to (68.17±16.79)% when direct lysis was used for viral elution, therefore direct lysis was proved much better than shaker, vortex and ultrasonication. They were significantly different (P<0.0001). Conclusions Elution process played the key role in HAV concentration when membrane filtration method was used. Direct lysis was proved much better than other method and it was the most efficient way in HAV recycle from mimicked water samples.

Objective: To investigate the efficacy and feasibility of ¹⁹²Ir high-dose rate brachytherapy for recurrent intrapulmonary oligometastasis after colorectal cancer surgery. Methods: Patients from May 2013 to October 2017 with intrapulmonary oligometastasisafter colorectal cancer surgery in Cangzhou Integrated Traditional Chinese and Western Medicine Hospital were enrolled. A total of 15 lesions were obtained from 10 patients, which were treated with CT-guided high dose rate of ¹⁹²Ir. The implant needles were inserted into the tumor and were adjusted to appropriate positions under the guidance of CT. Then the images after transplanting were uploaded to the planning system to delineate the target area and the organ at risk volume. Patients underwent a single radiation dose of 20 Gy. Results: All 10 patients were successfully treated. Grade 1 adverse events were observed for 30% of patients. Of the 10 patients, one patient had a mild cough, and two had bloody sputum. There was no serious adverse events occurred. The local control rate (LC) of the patients at 1 year after treatment was achieved in 93.3%. Only one developed local advancement after six months, who received the secondary brachytherapy. The median progression-free survival(PFS) was 8.5 months and the median overall survival(OS) was 14.7 months. Conclusions High dose rate brachytherapy is effective in terms of recurrent lung metastases after surgery for colorectal cancer, with a moderate rate of adverse reactions and a favorable local tumor control rate.