Circulating tumor cells (CTCs)are special kind of tumor cells in the peripheral blood of patient with tumor.Now CTCs detection has been used in the survival time prediction,post-operational recu-rrence detection,individualized treatment and other aspects in the patients with gastric cancer.As the research going,CTCs will provide new help for the comprehensive treatment of gastric cancer.

Objective To study the effects of mobile telephone microwave radiation on learning and memory of young and senile mice. Methods The young(5 months old) and senile(10 months old) male Kunming mice were randomly divided into 2 groups respectively, the control and exposed. The young and senile mice were settled in the environment of the mobile telephone signal shoots station (3 V/m) and exposed to the mobile telephone conversation radiation with two mobile telephones for 5 h/d for 50 consecutive days. The control group was in the environment without microwave radiation (0 V/m). The capability of learning and memory of each group were determined by Morris maze test(the time of finding the flat roof:the latency), the activity of acetylcholinesterase (AchE) in the hippocampus and temporal lobe were measured by spectrophotometry. Results With the increase of training time, no significant decrease of the latency was seen in each group. Compared with the senile control group, the activity of AchE in the hippocampus and temporal lobe of the young control group and the senile radiation group were lower(P

AIM:To explore the efficacy of Qingre Huayu Recipe in the treatment of non-proliferative diabetic retinopathy(NPDR).

METHODS:We selected 84 cases of 84 eyes of NPDR patients diagnosed as “heat-blocking type” in the Department of Ophthalmology of our hospital during June 2016 to December 2017. According to the random number table method, 42 cases were divided into control group and experimental group respectively. On the basis of conventional hypoglycemic treatment, the control group was treated with calcium hydroxy benzene sulfonate capsules, and the observation group was treated with Qingre Huayu Recipe for 6wk. The improvement of visual acuity, fundus lesions and clinical efficacy before and after treatment were observed.

RESULTS:Before treatment, there was no significant difference in visual acuity between the two groups(P>0.05). After 6wk of treatment, the visual acuity of the experimental group(0.21±0.11)was significantly better than that of the control group(0.30±0.13), and the difference was statistically significant(P<0.05). After 6wk of treatment, the fundus improvement efficiency and clinical efficacy(78.6% and 71.4%)were higher in the experimental group than in the control group(45.2% and 40.5%). There were significant differences in the improvement of fundus and clinical efficacy between the two groups(P<0.05).

CONCLUSION:On the basis of traditional hypoglycemic treatment, Qingre Huayu Recipe has good curative effect on NPDR(heat-blocking type), which can improve the prognosis of patients. It is in line with syndrome differentiation and treatment, and provides ideas for Chinese medicine treatment of NPDR.

AIM: To investigate the efficacy and safety of dexamethasone versus conbercept in the treatment of diabetic macular edema(DME)with different optical coherence tomography(OCT)subtypes.METHODS: A total of 160 DME patients(160 eyes)admitted to our hospital from January 2021 to March 2023 were prospectively selected, and the patients were randomly divided into dexamethasone intravitreal implant group and conbercept group, with 80 cases(80 eyes)in each group, and DME patients were divided into 51 eyes with serous retinal detachment(SRD), 55 eyes with cystoid macular edema(CME), and 54 eyes with diffuse retinal thickening(DRT)according to OCT characteristics. The best corrected visual acuity(BCVA), central macular thickness(CMT), intraocular pressure and adverse reactions were compared before treatment and at 2, 3 and 6 mo postoperatively.RESULTS: There were differences in BCVA, CMT and intraocular pressure between the two groups at 2, 3 and 6 mo compared with those before operation(all P<0.05). There were differences in BCVA, CMT and intraocular pressure between the dexamethasone intravitreal implant group and the conbercept group in the treatment of patients with different types of DME(all P<0.05). The BCVA of patients with DRT and SRD types in the dexamethasone intravitreal implant group was improved at 3 and 6 mo after treatment compared with that in the conbercept group(all P<0.05). At 6 mo after treatment, the CMT of patients with DRT type in the dexamethasone intravitreal implant group was lower than that in the conbercept group(P<0.05). During the follow-up period, none of the patients experienced adverse events such as cataract exacerbation or retinal detachment.CONCLUSION: Both dexamethasone intravitreal implant and conbercept treatment can improve visual function and macular retinal morphology in patients with different OCT subtypes of DME with good safety, but the dexamethasone intravitreal implant is better than conbercept in the treatment of DRT type.

Objective: To analyze the epidemiological characteristics and clinical features of the patients with 2019-nCoV infection, so as to provide basis for clinical diagnosis. Methods: The epidemiology, clinical symptoms, laboratory and radiologic data of 23 patients with 2019-nCoV infection admitted to the Fifth People's Hospital of Xinyang City from January 22,2020 to January 29, 2020 were retrospectively analyzed. Results: The 23 patients with 2019 nCov infection consisted of 15 men and 8 women, and the median age was 46.0 (40.5, 52.0) years (27-80 years); 9 of them had basic disease (39%), including hypertension (17%), cardiovascular diseases (17%), diabetes (9%), hypothyroidism (4%) and old tuberculosis (4%). All the 23 patients had contact history in Wuhan area or with confirmed infections. Clinical symptoms included: fever (100%), cough (70%), expectoration (43%), myalgia (26%), headache (17%) and dyspnea (17%), and the less common symptoms were diarrhea (4.3%). Blood routine test: white blood cells (WBC) < 4×10⁹/L in 11 cases (48%), (4-10)×10⁹/L in 10 cases (43%), >10 × 109/L in 2 cases (9%); lymphocytopenia in 13 cases (56%). All 23 patients had different degrees of infective lesions in chest CT examination, with 9 cases (39%) on one side and 14 cases (61%) on both sides. Classification: 19 mild cases, 4 severe cases, no critical or death case. Complications included acute respiratory distress syndrome [4 (17%)]. No case was reported with the damage of liver or kidney function and with secondary infection. Conclusions Epidemic history of contact, fever, pneumonia signs of chest CT, normal or decreased count of WBC and lymphocytopenia are the clinical basis for diagnosis of the disease. However, at present, the treatment of patients has not been completed, the effective treatment strategy and final prognosis are not clear.

OBJECTIVE To evaluate the quality of different germplasms of Rehmannia glutinosa based on iridoid glycosides. METHODS The contents of total iridoid glycosides ，catalpol，rehmaionoside D ，rehmaionoside A ，and leonuride in 18 batches of R. glutinosa from 6 germplasms（85-5，JinJiu，BX，BJ-1，Shandong，QH-1）were determined by ultraviolet spectrophotometry and high performance liquid chromatography. After normalization of the above content determination results ，the quality of different germplasm of R. glutinosa were evaluated by multiple statistical methods such as cluster analysis ，factor comprehensive analysis and partial least squares discriminant analysis （PLS-DA）. RESULTS Among 6 germplasms of R. glutinosa ，the content of total iridoid glycosides in R. glutinosa 85-5 was the highest ，and the content of catalpol in R. glutinosa BX was the highest ；the contents of rehmannioside D and rehmannioside A in R. glutinosa JinJiu were the highest ，and the content of leonuride in R. glutinosa BX was the highest. Cluster analysis showed that R. glutinosa JinJiu were clustered into one category ，R. glutinosa BX clustered into one category ，R. glutinosa Shandong and R. glutinosa BJ-1 were clustered into one category ，and R. glutinosa QH-1 and 85-5 were clustered into one category. Through factor comprehensive analysis ，there were differences in the quality of different germplasms of R. glutinosa . The comprehensive score of R. glutinosa BX，Shandong，85-5，BJ-1，QH-1，JinJiu were 2.283 9，1.689 1，1.664 8， 1.503 3，1.469 0，1.214 6，respectively. PLS-DA showed that variable importance projection value of total iridoid glycosides ， catalpol and leonuride were all higher than 1. CONCLUSIONS The quality difference of R. glutinosa from different germplasms may be caused by total iridoid glycosides ，catalpol and leonuride.

Objective To compare the influence of tracheotomy after two wet fluid on airway and provide the basis for clinical treatment and care. Methods A total of 30 patients with severe brain injury stay neurosurgery tracheotomy were divided into 0.45% sodium chloride group and ambroxol hydrochloride group with 15 cases each by random digits table method, two airway humidification liquid (0.45%sodium chloride,0.9% sodium chloride + ambroxol hydrochloride) were each instilled in the trachea inner sleeve. Blood gas analysis was performed and the levels of serum lung surface active substances related protein-A (SP-A protein), interleukin-6, interleukin-8, tumor necrosis factor-alpha(TNF-α) were measured by enzyme linked immunosorbent assay (ELISA) before 1 d and after 3,7,14 d of tracheotomy. Results There were significant differences in arterial blood oxygen partial pressure, arterial carbon dioxide partial pressure, oxygenation index after 14 d of tracheotomy between ambroxol hydrochloride group and 0.45% sodium chloride group:(110.72±26.75) mmHg(1 mmHg=0.133 kPa) vs.(89.39±21.98) mmHg, (30.44±6.75) mmHg vs. (35.12±7.28) mmHg, 333.23±80.56 vs. 270.93±77.21, t=29.49,-8.63,7.44, P<0.01.There were significant differences in the levels of serum SP-A protein, interleukin -6, interleukin -8, TNF-α after 14 d of tracheotomy between ambroxol hydrochloride group and 0.45% sodium chloride group:(191.34 ±1.21) ng/L vs. (61.92 ±12.0) ng/L, (2.62 ±0.23) ng/L vs. (5.42 ±0.16) ng/L, (124.56 ±2.10) ng/L vs. (185.91 ±1.48) ng/L, (31.32±1.38) ng/L vs.(69.13±1.16) ng/L, t=75.72,-13.51,-23.89,-20.97, P<0.01. Conclusions The airway humidification effect of ambroxol hydrochloride group is better than 0.45%sodium chloride group, it can improve the wetting effect, and better protect the lung tissue, reduce the incidence of lung infection, make it an ideal airway humidification liquid.

Objective: To observe the value of real-time three-dimensional echocardiography (RT-3DE) for evaluating left ventricular global and regional systolic function in children with left ventricular noncompaction (LVNC). Methods: According to left ventricular ejection fraction (LVEF) detected with conventional echocardiography, 34 children with LVNC were divided into LVEF normal group (group A, LVEF≥50%, n=25) and LVEF reduced group (group B, LVEF<50%, n=9), while 20 healthy children were selected as normal control group (group C) at the same period. RT-3DE examinations were performed, left ventricular end-systolic volume (LVESV), left ventricular end-diastolic volume (LVEDV) and LVEF were measured. The ratio of noncompaction myocardium to compaction myocardium (NC/C) at the thickest area on end-systolic count were calculated. The involvements of left ventricular 17-segment myocardium were observed. Regional end-diastolic volume (rEDV), regional end-systolic volume (rESV) and regional ejection fraction (rEF) were obtained with left ventricular volume-time curve analysis. The data were statistically analyzed. Results: There was no statistical difference of LVEDV (t=-0.17, P=0.87), LVESV (t=0.79, P=0.44) and LVEF (t=-1.72, P=0.10) between group A and C, nor of LVEDV (t=1.62, P=0.15) between group B and C. In group B, LVESV significantly increased (t=2.85, P=0.03), while LVEF significantly decreased compared with that in group C (t=-6.15, P<0.01). No statistical difference of NC/C was found between group A and B (t=-1.15, P=0.27), but the number of affected segments in group A was significantly fewer than that in group B (t=-4.59, P=0.03). There was no statistical difference of rEDV and rESV in all of 17 segments of myocardium in left ventricular between group A and C (all P>0.05), while rEF of apical septal, inferior and lateral segments, as well as med inferoseptal, inferior and inferolateral segments were significantly different between group A and C (all P<0.05). There was no significant difference of rEDV in all 17 segments of myocardium in left ventricular between group B and C (all P>0.05), while rESV of basal inferolateral and anterolateral segments, med anterior, anteroseptal, inferior, inferolateral and anterolateral segments, apical anterior, septal and lateral segments, as well as rEF in all 17 segments of myocardium in left ventricular were significantly different between group B and C (all P<0.05). Conclusion: RT-3DE can be used to evaluate the regional and global systolic function, which is helpful to early diagnosis, treatment and prognosis of LVNC children.

Female ; Humans ; Incidence ; Infant ; Male ; Oxygen ; metabolism ; Prospective Studies ; Pulmonary Edema ; diagnosis ; epidemiology ; Respiration ; Respiration, Artificial

OBJECTIVE： To prepare Capsaicin solid dispersion， to optimize its formulation and characterize the dispersion. METHODS： Capsaicin solid dispersion was prepared by melting method using P188 or PEG4000 as carrier. Using 60 min accumulative dissolution rate in vitro as index， orthogonal test was used to optimize type of carrier， drug ratio and stirring time. Validation test was conducted. The stability of Capsaicin solid dispersion within 0， 30， 180 d was investigated at 40 ℃ under the relative humidity of 75％， and X-ray diffraction （XRD） was used to analyze whether there were peaks. Capsaicin solid dispersion was characterized by differential scanning calorimetry （DSC） and XRD. RESULTS： The optimized formula included P188-PEG4000 as carrier， capsaicin-P188-PEG 4000 mass ratio of 1 ∶ 5 ∶ 3， stirring for 20 min. Accumulative dissolution rate in vitro of Capsaicin solid dispersion was 84.6％ within 60 min （n＝3）， and the preparation kept stable within 180 d [RSD of content was 3％ （n＝3）； XRD showed that there was no peak]. The phase characteristics showed that capsaicin was highly dispersed in the amorphous or molecular state in carrier. CONCLUSIONS： The optimized technology can be used to prepare Capsaicin solid dispersion， and improves more than 80％ dissolution rate in vitro at 60 min. It is stable， simple and feasible.