Objective To investigate the protective effect and possible mechanism of Decorin on pulmonary fibrosis.Methods A total of 90 healthy Wistar rats were randomly divided into three groups:group A served as control group,group B was injected BLMA5 through trachea,group C was injected BLMA5 and received treatment of Decorin.Ten rats in each group were killed 7、14、28 days after intratrachael instillation.TGF-?_1 expression was measured by immunohistochemical technique.The degree of alveolitis and fibrosis were measured by HE staining.The contents of hydroxideproline were measured in the homodenate of the rat lung.Results The expressions of TGF-?_1 were increased gradually in group B;in group C the expressions were decreased significantly than group B(P

Objective Desflurane is now well accepted by anesthesiologists because of its rapid induction and recovery but carboxyhemoglobin(CUHb) formation from interaction of desflurane with soda lime is a major concern. The purpose of this study was to investigate the effects of different fresh gas flow(FGF) rates on COHb formation during desflurane anesthesia. Methods Forty ASA Ⅰ-Ⅱ nonsmoking patients undergoing elective surgery were randomly divided into four groups with ten patients in each group according to FGF rate: group Ⅰ 0.5L/min; groupⅡ 1.0L/min; group Ⅲ 2.0L/min; group Ⅳ4.0L/min. The patients were premedicated with pethidine 1mg/kg and atropine 0.01mg/kg. Anesthesia was induced with midazolam 0.05mg/kg, propofol 2mg/kg and fentanyl 7pg/kg. Intubation was facilitated with succinylcholine. Anesthesia was maintained with desflurane( 1 .5 MAC), nitrous oxide( 50%) and intermittent vecuronim and fentanyl. PET CO2 was maintained between 35-40 mmHg. Venous blood samples were taken before and 2h, 4h and 6h after induction of anesthesia and at the end of operation for determination of COHb level. Results There was no significant difference among the four groups in age, gender and weight. COHb concentration was not significantly different before and 2h after induction of anesthesia among the four groups, but increased significantly at 4h and 6h after induction of anesthesia and at the end of surgery in groupⅣ, and tended to increase as the operation was prolonged. It was found that patients who developed postoperative headaehe and PONV were mostly from group IV, but no patients developed delayed neuropsychologic sequelae. Conclusions COHb level increases with high FGF rate but not with low FGF rate because of dryness and higher temperature of soda lime. Fresh soda lime should not be left in Canister too long and should be replaced shortly before anesthesia.

005), but NO level declined remarkably 90 min and 120 min following inhalation(P

Objective To evaluate the effects of applying XF-9801 type of anesthetics waste gas absorber, an activated carbon filter,to removing nitrogen oxides (NOx) and fluoride. Methods Thirty patients, undergoing general anesthesia with enflurane or isoflurane/nitrous oxide, were enrolled in this study.The gas samples were taken at the waste gas outlet of anesthetic machine,to identify the concentrations of nitrogen oxides and fluoride before and after the application of anesthetics waste gas absorber. Results Compared with those before the absorber application, nitrogen oxides and fluroide concentrations in anesthetic waste gas decreased significantly following the absorber application (P

Objective To establish the analytical method for the fingerprint of the volatile components in the root tuber of Pseudostellaria heterophylla by GC-MS and provide the basis for quality assessment of the crude drug.Methods The volatile components in the root tuber of P.heterophylla from different habitats were analyzed and the chromatographic fingerprints were established by GC-MS.The common peaks were determined and the fuzzy cluster was selected to compare the results.Results There were 12 main characteristic components in the volatile components in the root tuber of P.heterophylla.GC-MS Fingerprint of 12 common peaks was established preliminarily.Conclusion The method is reliable and accurate,and can be used for quality control of the root tuber of P.heterophylla.with favourable reproducibility.

Objective To identify the value of multiparametric MR imaging features including diffusion weighted (DW) and dynamic contrast-enhanced (DCE) MR imaging features in differentiation between inverted papilloma and malignant transformation of inverted papilloma in the sinonasal region.Methods Seventy-seven patients were included in this study,including 12 malignant transformation of inverted papilloma and 65 inverted papillomas.Conventional MRI including nonenhanced and static contrast-enhanced imaging,DCE-and the DW-MRI were analyzed.Differences in conventional MRI features,ADCs and DCE-MRI parameters between the two entities were determined by Fisher exact test,independent samples t-test and Mann-Whitney U test,respectively.Results There were significant differences in convoluted cerebriform pattern (P=0.045),necrosis (P=0.003) and orbit involvement (P＜ 0.01) between inverted papilloma and malignant transformation of inverted papilloma in the sinonasal region.The ADCs of inverted papilloma were significantly lower than those of malignant transformation of inverted papilloma (P＜0.01).There were significant differences in time to peak enhancement (P＜0.01),maximum contrast index (P=0.004) and time intensity curve types (P＜0.01) between the two entities.Conclusions A multiparametric approach using conventional MR imaging with added ADCs and DCE-MRI parameters had the potential to improve the differentiation between inverted papilloma and malignant transformation of inverted papilloma in the sinonasal region.

Objective To investigate the reliability and effect of using the chimeric flap retrieved by laparoscopic surgery to cover the large defect of the extremities.Methods The debridement and vacuum sealing drainage (VSD) were performed on 18 patients, who were admitted due to the defects of the extremities.The free chimeric peritoneal-deep inferior epigastric artery perforator (DIEP) flap assisted by laparoscopic surgery was transplanted to cover the defect with exposed tendons and/or skeleton.Results The remaining defects of the extremities were 12 cm × 8 cm-30 cm × 17 cm.The peritoneal component of the chimeric flaps measuring 8 cm × 6 cm -14 cm × 10 cm retrieved by laparoscopic surgery was used to cover the tendons, bones and joints.The deep inferior epigastric artery perforator (DIEP) flaps measuring 13 cm × 10 cm-32 cm × 18 cm allowed the cutaneous coverage of wounds.The chimeric flaps survived completely excepting two patients.The two patients experienced partial necrosis of the chimeric flaps and received skin grafting to achieve the wound closure.The function of the injured extremities recovered partially after 6-18 months of follow-up.No abdominal pain, distension, herniation,bulging and intestinal obstruction were recorded.Conclusion The chimeric flap assisted by laparoscopy is a helpful, safe and effective method for reconstruction of large wounds in extremities with exposed tendons and bones.

Anesthesia safety plays an important role in the medical safety of the perioperative.At present the medical institutions in many countries apply crew resource management model for training and assessment in anesthesiology,surgery and emergency.It emphasizes the training of nontechnical skills,called crisis resource management(CRM).This article analyzes the causes of human error incidents and preventive measures and introduces CRM principles in medical principles and anesthesia correlation and applications.CRM helps to foresee the crisis of environment and deal with the crisis source to ensure the safety of patients.

Purpose To discuss the TCR gene rearrangements in the diagnosis of T-cell lymphomas. Methods Formalin-fixed and paraffin-embedded samples including 30 cases of T-cell lymphomas and 30 cases of reactive lymphoid hyperplasia were chosen for ex-tracting genomic DNA and PCR amplification using 56 BIOMED-2 primers. PCR products were analyzed by heteroduplex and polyacryl-amide gel electrophoresis. Results In all 30 cases of T-cell lymphomas, 25 cases (83. 3%) showed TCRβ gene monoclonal rear-rangements, 28 cases (93. 3%) of TCRγ gene monoclonal rearrangements, 4 cases (13. 3%) of TCRδ gene monoclonal rearrange-ments. 29 cases (96. 7%) with TCRβ+TCRγ+TCRδ gene monoclonal rearrangements were detected. but no clonal TCR gene rear-rangements were found in 30 cases of reactive lymphoid hyperplasia. Conclusions The detection of TCR gene rearrangements using BIOMED-2 primers is a useful assistant method for the diagnosis of T-cell lymphomas.

Objective To explore the curative effect and relative nursing care key points of Nd∶YAG laser assisting the drug treatment for patients with recurrent oral ulcer.Methods Toally 82 patients with recurrent oral ulcer treated in our department were selected and alternately divided into experiment group (41 cases) and control group (41 cases) according to the initial visiting sequence with same gender.The control group used the conventional drug treatment.On the basis of conventional drug treatment,the experiment group additionally used the Nd∶YAG laser radiation therapy.The curative effects of both groups were evaluated after relative treatment for 4 days.Results The total effective rates of curative effects of two groups had significantly statistical differences (P＜0.001).The total effective rate of the experiment group was,significantly higher than that of the control group.Conclusions Nd∶YAG laser assisting the drug treatment for the patients with recurrent oral ulcer can significantly increase the total effective rate.The correct nursing services throughout the operative process are the important guarantee of good curative effect.