1.The progress of lung ultrasound application in children′s lung diseases
Xinyan CHANG ; Xiaoyu XIONG ; Chengjun LIU
Chinese Pediatric Emergency Medicine 2021;28(3):220-223
Lung ultrasound has been proven to have high sensitivity and specificity in the application of lung diseases, and significantly reduces the radiation exposure of children, especially in newborns, which makes it widely used in diagnosis, treatment and prognosis of childhood disease.In emergency and intensive care units, lung ultrasound can even replace chest radiographs as a routine examination method for chest lesions.However, the research of children′s lung ultrasound still falls behind the development of adult ultrasound, and there is a lack of standard procedures and specifications for children′s lung ultrasound.Therefore, this study mainly reviewed the research status of children′s lung ultrasound.
2.Monitoring novel ovarian carcinoma associated genes using cDNA expression microarray
Xiaohong CHANG ; Yexia CHENG ; Xinyan ZHANG ; Heng CUI ; Jie FENG ;
Chinese Journal of Obstetrics and Gynecology 2001;0(06):-
Objective To explore the gene expression pattern of sample of human ovarian carcimoma Method The difference in gene expression between normal and neoplastic human ovarian tissues were investigated,we described the assembly and utilization of a 512 member cDNA microarray Result Thirty seven genes expressed in ovarian cancer were screened out,14 genes were up regulated,23 genes were down regulated Conclusion cDNA microarray for analysis of gene expression pattern is an effective method to identify novel ovarian cancer associated genes
3.Protective effects of Jiedu Tongluo Fang on rats with diabetic cardiomy-opathy
Xinyan WANG ; Liping CHANG ; Yue DENG
Journal of Beijing University of Traditional Chinese Medicine 2017;40(8):669-676
Objective To explore the possible mechanism of Jiedu Tongluo Fang(Toxin-removing collat-eral-unblocking Formula,JDTLF)in the treatment of diabetic cardiomyopathy by observing its effects on the expression of peroxisome proliferator activated receptor gamma(PPARγ)and cardiac function in rats with diabetic cardiomyopathy.Methods 100 Wistar rats were randomly divided into normal control group (n =8)and diabetic model group (n =92).Diabetic model was built by using intraperitoneal in-jection of streptozotocin (STZ,55 mg/kg).Diabetic cardiomyopathy(DCM)model was built by feeding those rats for another 12 consecutive weeks.DCM model rats were randomly divided into JDTLF group, Qingre JieduYao(heat-clearing toxin-removing medicinal,QRJDY)group,pioglitazone group,and DCM model group.Except the DCMmodel group and the normal control group,the other 3 groups were orally gavaged with 10.4 g/(kg·d)JDTLF,2.7 g/(kg·d)QRJDY,and 2.7 mg/(kg·d)pioglitazone re-spectively.DCMgroup and NC group received distilled water for 6 weeks.At the end of the experiment, random blood glucose (RBG),blood lipid(TC,TG,LDL-C,and HDL-C),high sensitive C reactive protein (hs-CRP),cardiac function(LVSP,LVEDP,+dp /dtmax ,-dp /dtmax and t-dp /dt)and morphologi-cal changes were evaluated.Expression of PPARγin myocardium was measured with immunohistochemis-try.Results Compared with the NC group,body weight in DCM group decreased significantly (P <0.01);Random blood glucose,hs-CRP,TC,TG and LDL-C increased significantly (P <0.01,P <0.05);HDL-C decreased significantly (P <0.05),and cardiac function decreased significantly;LVSP,+dp /dtmax ,-dp /dtmax decreased significantly;LVEDP and t-dp /dt increased significantly (P <0.05). There was a significant decrease in the expression of myocardial PPARγ;Compared with DCM model group,random blood glucose in JDTLF group and QRJDY group decreased significantly;Hs-CRP,TC, TG and LDL-C decreased significantly (P <0.01,P <0.05 ),HDL-C increased significantly (P <0.05);Cardiac function improved in JDTLF group and QRJDY group;LVSP,+dp /dtmax and -dp /dtmax increased significantly (P <0.05);LVEDP and t-dp /dt decreased significantly (P <0.05);Expression of myocardial PPARγpresented an increasing tendency (P <0.01).Conclusion Jeidu Tongluo Fang and Qingre Jiedu Yao can significantly improve the glucose and lipid metabolism,heart function and heart tissue structure,and have protective effect on diabetic cardiomyopathy.Its mechanism may be related to the up-regulation of PPARγ.