Objective: To investigate the efficacy of Shensuyin in treating viralmyocarditis (VMC) with syndrome of insufficiency of lung-qi and the effect on levels of Th17 and Treg cells and relevant factors. Method: One hundred-four VMC cases were regaded as object of study and randomly divided into control group and observation group, with 52 cases in each group. Control group was treated with routine therapy by reference to ‘Chinese Guidelines for Diagnosis and Treatment of Heart Failure 2014’. In addition to the therapy of control group, observation group on the basis of treatment in the control group with Shensuyin, 1 dose/d, bid. One course of treatment was 8 weeks for both groups. Scores of Shensuyin, serum levels of Troponin I (cTnI) and cardiac free fatty acid binding protein (H-FABP), heart function and total efficacy were compared for both groups. Flow cytometry was used to detect peripheral blood levels of Th17 and Treg cells for the two groups. Serum levels of interleukin (IL) -17, IL-21, IL-10 were detected in both groups. Result: After treatment, scores of syndrome of Yin and Yang deficiency (shortness of breath, fatigue, dull chest pain, bad breath, cough) of observation group were obviously lower than those of control group (P<0.01). Serum levels of H-FABP and cTnI of observation group were remarkably lower, while LVEF and E/A were higher than those of control group after treatment (P<0.01). Total efficacy of observation group was 92.16%, which was higher than 74% of control group (P<0.05). After treatment, peripheral blood levels of Th17 cell and Th17/Treg were obviously lower, while Treg cell was higher thancontrol group (P<0.01). Serum level of IL-17, IL-21 of observation group was remarkably lower, while IL-10 was higher than control group after treatment (P<0.01). Conclusion: In addition to the routine therapy of VMC, the efficacy of Shensuyin has a significant effect in treating VMC with syndrome of lung qi deficiency, and the regulatory effect on levels of Th17 and Treg cells and relevant factors may be one of the effective ways.

Objective:To observe the clinical efficacy of Shugan granule on perimenopausal depressive disorder (stagnation of liver Qi and Qi stagnation) and its effect on sex hormone and inflammatory factors. Method:One hundred and twenty patients were randomly divided into control group (60 cases) and observation group (60 cases) by random number table. Both groups got Tibolone tablets, 2.5 mg/time, 1 time/day. Patients in control group got flupentixol and melitracen tablets once every morning and noon, 1 tablet/time. In addition to the therapy of control group, patients in observation group got Shugan granule, 1 pack/time, 2 times/days. And a course of treatment was 8 weeks. Main indexes were graded by Hamilton depression scale (HAMD-17), self-rating depression scale (SDS) and Eysenck Personality Questionnaire (EPQ). And secondary indexes were scored by self-rating anxiety scale (SAS), stagnation of liver Qi and Qi stagnation, improved kupperman (KI), levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E₂), 5-serotonin (5-HT), brain derived neurotrophic factor (BDNF), interleukin-1β (IL-1β), IL-6 and tumor necrosis factor-α (TNF-α) before and after treatment. Result:By rank sum test, the clinical efficacy on depressed and perimenopausal syndrome in observation groups was better than that in control group (Z=2.038, P<0.05, Z=2.316, P<0.05).Scores of HAMD-17, SDS, EPQ-N, SAS, KI and stagnation of liver qi and qi stagnation were lower than those in control group (P<0.01), whereas score of EPQ-E was higher than that in control group (P<0.01). And levels of FSH, LH, IL-1β, IL-6 and TNF-α were lower than those in control groups (P<0.01). Levels of E₂, BDNF and 5-HT were higher than those in control groups (P<0.01). Conclusion:Shugan granule can relieve depression, inquietude and symptoms caused by perimenopausal syndrome, regulate sex hormone, 5-HT, BDNF and inflammatory reaction, with a better clinical effect than pure western medicine treatment.

OBJECTIVE: To measure the implicit attitude of nurses towards doctors and analyze its influencing factors. METHODS: A total of 356 nurses were selected as study subjects by convenient sampling method. Their implicit attitude towards doctors was measured by Single Category Implicit Association Test. The related influencing factors were analyzed. RESULTS: The response time of nurses on incompatible tasks was higher than that on compatible tasks [(0.87±0.19) vs(0.76±0.15) s,P<0.01]. The median(M) of D value was 0.34. D value of junior college group was higher than that of undergraduate and above group(M: 0.40 vs 0.27, P<0.01). D value in the nurse group was higher than that in the nurse practitioner group and nurse-in-charge and above group(M: 0.43 vs 0.33, 0.43 vs 0.23, P<0.05). D value of the informal staff group was higher than that of the formal staff group(M: 0.35 vs 0.19, P<0.05). The results of ordered multiple Logistic regression analysis showed that establishment was an independent influencing factor of the implicit attitude of nurses towards doctors(P<0.05), after excluding the influence of confounding factors. CONCLUSION: The implicit attitude of nurses towards doctors is positive and mainly affected by the establishment.

To investigate the expression and significance of cell surface receptor signaling lymphocyte activation molecule family member 7 (SLAMF7) in normal intestinal tissues and intestinal inflammatory tissues of mice.

Five C57BL/6J wild-type male mice aged 8-10 weeks were chosen and fed normally. Lamina propria lymphocytes (LPLs) and intestinal epithelial cells (IECs) were extracted, and totalcell RNA was extracted by Trizol reagent. The mRNA expression of SLAMF7 in cells was detected by reverse transcription polymerase chain reaction (RT-PCR) and real-time fluorescent quantitative polymerase chain reaction (RT-qPCR). Then 10 C57BL/6J wild-type male mice aged 8-10 weeks were randomly divided into control group (n=5) and model group (n=5). The control group was fed with normal drinking water, whereas the model group was fed with 2.5% dextran sodium sulfate (DSS) drinking water to establish a model of ulcerative colitis (UC). The mice in both groups were killed on the 5th day. Flow cytometry was used to detect the expression of SLAMF7 in immune cell subpopulations in the control group and the model group.

Compared with colonic IECs, the expression of SLAMF7 was higher in colonic LPLs(P=0.017). After DSS induced enteritis, the expression of SLAMF7 was up-regulated in neutrophils (P=0.001), but had no significant changes in CD4⁺T cells, CD8⁺T cells, CD19⁺B cells, macrophages and conventional dendritic cells(all P > 0.05).

SLAMF7 may play an important role in the occurrence and development of UC through neutrophil-related pathways.

Objective: To understand the relationships between college students’ aggression and self-acceptance, family intimacy and adaptability, and to provide reference for college students’ aggression preventing and intervening. Methods: Using general information questionnaire, Aggression Questionnaire, Self-acceptance Questionnaire and FACES II-CV, 984 college students from 3 colleges in Xinxiang, Henan province were surveyed by questionnaire. Results: The total score of physical aggression, verbal aggression, indirect aggression and aggression in male students was higher than that of female students (t=7.17,4.21,2.05,3.63,P<0.05).The score of physical aggression of only children was higher than that of nononly children (t=2.39,P<0.05).The score of indirect attack of disciplined college students was higher than that of undisciplined college students (t=2.60,P<0.01).There were statistically significant differences in the total scores of indirect attack, hostility and attack among college students with different left-behind experiences (F=3.39,4.61, 3.37, P<0.01).There were statistically significant differences in the total scores of physical aggression, anger and aggression among college students by family income(F=5.70,3.94,3.37,P<0.01).Correlation analysis showed that the total score of college students’ aggression was positively correlated with self-acceptance, negatively correlated with actual family intimacy and actual family adaptability (r=0.37，-0.09，-0.07,P<0.01). Regression analysis showed that self-acceptance, gender, disciplinary action, left-behind experience and expected family adaptability showed significant associations with total score of college students’ aggression (P<0.05). Conclusion Self-acceptance and family closeness and adaptability are associated with aggressive behavior in college students. Schools should carry out targeted psychological health education on self-acceptance to ensure that college students maintain a reasonable level of self-acceptance, and family members should develop certain level of intimacy and adaptability, in order to reduce the occurrence of aggressive behavior of college students.

Objective　Lipoxin A4 (LXA4) has been proved to have a good protective effect on spinal cord ischemia-reperfusion injury in rats, but whether autophagy is one of the protective mechanisms remains unclear. This study aims to investigate the effects of lipoxin A4 on rat spinal cord ischemia-reperfusion injury.　Methods　48 rats were randomly divided into LXA4 group, ischemia-reperfusion group (SCII group) and sham group with 16 rats in each, and the models of each group were built accordingly. The rats in LXA4 group received intrathecal injection of 10μl LXA4 (300 pmol) 30 minutes after clamping the abdominal aorta. Three groups of rats were sacrificed by cervical dislocation 24 hours after reperfusion and the apoptosis-positive cells were then obtained. The spinal cord tissues of three groups of rats were stained and counted by LC3B fluorescence staining, and the expressions of LC3-II/LC3-I and GABARAP protein were detected by Western blot.　Results　There were few LC3B positive cells in the sham group. Compared to those in the sham group (73.40±19.42), the number of LC3B positive cells in SCII group (399.80±18.46) and LXA4 group (240.80±12.76) significantly increased (P<0.05), and the number in LXA4 group was significantly lower than that in SCII group (P<0.05). The ratio of LC3-II/LC3-I and the expression of GABARAP in SCII group and LXA4 group was significantly higher than those in sham group (P<0.05). The ratio of LC3-II/LC3-I in spinal cord tissue significantly declined compared with that of SCII group (P<0.05).　Conclusion　The autophagy is activated when SCII occurs, indicating that the autophagy is involved in SCII. After LXA4 is administered, autophagy is inhibited and SCII is alleviated.

Coronary disease is one of the highest mortality diseases in the world,and interventional therapy has been the best treatment choice for its low risks,high efficiency,less wound and rapid recovery after the operation.Thrombus aspiration catheter is one of the most important equipment in the interventional therapy instrument of coronary disease.This paper is based on the demand of clinical and market,designed and manufactured aspirated catheter for the treatment of coronary thrombosis.Through the performance comparison of the material,confirmed the main material quality of thrombus aspiration catheter and its organization.We also made the appraisement for the function of the material and the main performance of the thrombus aspiration catheter.The experiment turned out that our catheter performance is stable and also with highly reliable,which is absolutely fit for the using requirements of the clinical.
Catheters ; Coronary Angiography ; Coronary Thrombosis ; therapy ; Humans ; Suction ; Thrombectomy ; instrumentation ; Treatment Outcome

OBJECTIVETo examine the association between CLOCK gene T3111C polymorphism with attention deficit hyperactivity disorder (ADHD) and ADHD related sleep disturbances in children.

METHODSOne hundred and sixty-six unrelated children with ADHD diagnosed according to DSM-IV criteria and a control group of 150 normal children were enrolled in this study. Parents filled out the Sleep Disturbance Scale for Children (SDSC). Genotype and allele frequencies of T3111C of the CLOCK gene were examined by PCR-restriction fragment length polymorphisms (PCR-RFLP).

RESULTSThere were significant differences in the genotype and allele frequencies of T3111C of the CLOCK gene between the ADHD and control groups (P<0.05). C allele frequency in the ADHD group was significantly higher than in the control group (χ2=7.254, P=0.007, OR=1.740, 95%CI=1.160-2.612). The ADHD children with sleep disturbances were found to have higher C allele frequency than those without sleep disturbances (χ2=13.052, P<0.001, OR=2.766, 95%CI=1.573-4.865).

CONCLUSIONSThere is an association between CLOCK gene T3111C polymorphism and both ADHD and related sleep disturbances in children. The individuals with C allele are susceptible to ADHD as well as ADHD related sleep disturbances.

Adolescent ; Attention Deficit Disorder with Hyperactivity ; complications ; genetics ; CLOCK Proteins ; genetics ; Child ; Child, Preschool ; Female ; Humans ; Male ; Polymorphism, Genetic ; Sleep Wake Disorders ; etiology ; genetics

Objective: To investigats the specific changes of brain neuron oscillation in non-clinical high-trait anxiety college students by recording the resting state EEG of high and low trait anxiety subjects. Methods: College students in a university were administered by using the S-TA Inventory, 27% of the number of people before and after the selection were divided into low-specific focus group (15) and high-trait anxiety group(15), based on the STAT score. After pre-processing, the data was divided into five frequency bands of δ(1-<4), θ(4-<8), α(8-<13), β(13-<30), γ(30-100)Hz and every electrode power value of those was calculated respectively. Correlation between power spectrum and trait anxiety scores was investigated. Results: The high-trait anxiety group were in the frontal and central regions (t=3.47, 2.62) of the δ band, the frontal region (t=2.22) of the θ band, the frontal, central, right temporal, and posterior regions (t=2.77, 2.23, 3.65, 2.35) of the β band, the frontal, left temporal, central, right temporal, and posterior regions (t=2.83, 2.22, 2.64, 2.43, 2.09) of the γ band, than that in the low trait anxiety group. Furthermore, in central regions of the δ band; the frontal region of the θ band; the frontal, the central, and posterior regions of the β band; the frontal, left temporal, central, and posterior regions(r=-0.63, -0.51, -0.62, -0.53, -0.54, -0.59, -0.56, -0.55, -0.49) of the γ band, the correlation between trait anxiety scores and the power value were obvious negatively. Conclusion High trait anxiety college students have lower power spectrum than low trait anxiety college students. The degree of trait anxiety is related to the power spectrum. The changes of brain resting-state electrical signals in high-trait anxiety individuals may be related to the influence of trait anxiety on college students’ attention and working memory.

Objective: To explore latent classes of college students aggressive behavior and its correlations with family care and the meaning of life among college students. Methods: A cross sectional questionnaire survey was conducted among 5 094 students from three universities in Xinxiang City in December 2021, using Aggression Questionnaire (AQ), Family APGAR Index (APGAR), and the Meaning in Life Questionnaire (MLQ). Results: Aggressive behavior of college students was classifed into three potential categories:low aggressive behavior group (26.4%), moderate aggressive behavior group (48.5%), and high aggressive behavior group(25.1%). There were significant differences in latent classes of aggressive behavior among college students by gender, physical flexibility, exercise frequency, and sleep status ( χ 2=63.95, 169.86, 125.76, 325.24, P <0.01). There were significant differences in the sense of life meaning and the degree of family care among the 3 potential categories of aggressive behavior ( F=113.47, 231.82, P <0.01). The multivariate Logistic regression analysis showed that the sense of meaning of life ( OR =0.96, 95% CI =0.96-0.97) and family care ( OR =0.83, 95% CI =0.81-0.84) were significantly associated with three classes of aggressive behavior ( P <0.01). Conclusion Aggressive behavior among college students is associated with gender, exercise frequency, sleep status, meaning of life, family care, and physical flexibility. School, family and society should actively pay attention to students psychological characteristics and to provide corresponding support for aggressive behavior prevention and intervention.