China ; Humans ; Pathology ; trends

Objective: To study the effects of antisense epidermal growth factor receptor (EGFR) cDNA transfection on telomerase activity and telomere length of glioblastomas U87MG cells and the mechanism. Methods: Glioblastoma U87MG cells, which over-expressed EGFR, were transfected with antisense-EGFR cDNA constructs. Several clones stably expressing lower or undetectable levels of EGFR protein were obtained. The effect of antisense-EGFR cDNA on telomerase activity was assessed by Telomeric Repeat Amplification Protocol (TRAP) assay. The effect of antisense- EGFR cDNA on telomere length was determined by Southern blot hybridization. The mRNA expressions of hTERT, hTEP1 and c-myc were analyzed by semi-quantitative PCR. Results: U87MG cells that were transfected with antisense-EGFR cDNA expressed lower level of EGFR and were less responsive to the growth stimulation of EGF compared with the control cells. Telomerase activity was significantly decreased in the antisense-EGFR clones. Telomere length was shortened. The mRNA expression of hTERT was slightly decreased in the antisense-EGFR clones, whereas the expressions of hTEP1 and c-myc were not altered. Conclusion: Antisense-EGFR cDNA transfection can sufficiently inhibit EGFR signal transduction pathway, decrease telomerase activity and shorten telomere length, which may be a new mechanism of antisense-EGFR approach in tumor suppression. The down-regulation of hTERT mRNA may contribute to the decreased telomerase activity in the antisense-EGFR clones.

OBJECTIVE: To evaluate the curative effect of endoscopic sinus surgery (ESS). METHOD: Two hundred and fifty-six cases with chronic sinusitis and nasal polyps, undergone ESS from 2001 to 2007 were analysed. There were 106 cases in type 1, 134 cases in type 2 and 16 cases in type 3. Two hundred and forty-six cases undergone ESS with intranasal local anesthesia and potentialized anesthesia. Long-time follow-up was performed at 9 to 42 months (mean = 15 months). RESULT: The results showed that cure rate was 78.3% for type 1, 66.4% for type 2 and 43.8% for type 3. There was significant difference in cure rate between type 1, type 2 and type 3 (P < 0.01). Complication of ESS of 256 cases was 4.3%. CONCLUSION There was higher efficacy and safety for relief of nasal polyps and for relief of chronic sinusitis with ESS. The difference of clinical type in nasal polyps and chronic sinusitis affected directly surgical result.
Adolescent ; Adult ; Aged ; Chronic Disease ; Endoscopy ; methods ; Female ; Humans ; Male ; Middle Aged ; Nasal Polyps ; surgery ; Sinusitis ; surgery ; Treatment Outcome ; Young Adult

Several key problems were analysed for the transdermal noninvasive glucose monitoring. A modified calibration equation was proposed for the high-sensitivity glucose biosensor due to its narrow linear range. The new equation has increased the sensors' linear range by 20 times. A new diffuse model was constructed for the electrode system of glucose sensor,aiming at the unique "finite space" electrochemical problem in trans-dermal technique. In addition,electrode masks were utilized to solve the problem of electrode loss in longtime glucose monitoring. In animal in-vivo experiments,70.1 % of the noninvasive glucose data points were clinically accurate,while the remains were clinically acceptable. All solutions mentioned above were based on both theoretical analysis and experimental validation,promoting the realization and optimization of transdermal noninvasive glucose monitoring techniques.

OBJECTIVETo study the effects of wild-type PTEN on gene expressions of glioblastomas.

METHODSGlioblastoma U87MG cells, which express inactivated PTEN, were transfected with wild-type PTEN constructs and stable transfected clones were selected. Then, cDNA microarray analyses were used to identify differentially expressed genes in wild-type PTEN transfected cells and control cells.

RESULTSTransfected wild-type PTEN inhibited the proliferation of U87MG. By cDNA microarray analyses, 89 cDNA clones were identified, which were differentially expressed in wild-type PTEN transfected cells and control cells. Among these genes, 13 genes were unknown and 76 genes were known genes, including glial fibrillary acidic protein, p21/WAF1, human TGF-beta inducible early protein, human DNA fragmentation factor 45 etc.

CONCLUSIONWild-type PTEN can affect the expressions of multiple genes, by which it regulates the proliferation, differentiation and apoptosis of glioblastomas.

Brain Neoplasms ; genetics ; Gene Expression ; Gene Expression Regulation, Neoplastic ; Glioblastoma ; genetics ; Humans ; Oligonucleotide Array Sequence Analysis ; PTEN Phosphohydrolase ; Phosphoric Monoester Hydrolases ; genetics ; physiology ; Transfection ; Tumor Cells, Cultured ; Tumor Suppressor Proteins ; genetics ; physiology

Objective To study the effects of single nucleotide polymorphisms (SNP) in Plasminogen activator inhibitor 1 (PAI-1) on breast cancer susceptibility and patients' prognosis among a Chinese Han women population.Methods Six tag SNP (tSNP) of PAI-1 were selected according to HapMap CHB population,and TaqMan realtime PCR method was used to genotype the 6 tSNP in 1 160 breast cancer cases and 1 318 age-matched controls among Chinese Han women.Haplotypes and diplotypes were inferred according to genotyping data and linkage disequilibrium.Finally,the associations of tSNP,haplotypes and diphypes with breast cancer susceptibility and patients' prognosis were analyzed.Results Regarding to breast cancer susceptibility,for rs6090 (G ＞ A),AA genotype carriers had 3.79 times higher risk of develoPing breast cancer (OR =4.79,95％ CI =1.01-22.64,P =0.048 0) than GG or GA genotype carriers.For rs2227672 (G ＞ T),TT genotype carriers had 1.52 times higher breast cancer risk than GG or GT genotype carriers (OR =2.52,95％ CI =1.26-5.01,P =0.008 6).Regarding to breast cancer prognosis,women who carried rs2227692 (C ＞ T) CT genotype had 46％ lower risk of developing recurrence,metastasis or death than CC genotype carriers (HR =0.54,95％ CI =0.30-0.97,P =0.040 4).Using stratified association analysis,among BMI ＜ 23 patients,those women who carried AA genotype of rs2227631 (G ＞ A) had 3.99 times higher risk of developing the events (recurrence,metastasis or death) than GG or GA genotype carriers (HR =4.99,95％ CI =1.66-15.02,P =0.004 2).Among HER2 positive patients,those women who carried AA genotype of rs2227667 (G ＞ A) had 2.98 times higher risk of developing the events (recurrence,metastasis or death) than GG or GA genotype carriers (HR =3.98,95％ CI =1.47-10.80,P =0.006 7).Among patients with tumors ＞ 2 cm,those women who carried rs2227692 (C ＞ T) CT or TT genotype had 51％ lower risk of developing the events (recurrence,metastasis or death) than CC genotype carriers (HR =0.49,95％ CI =0.27-0.88,P =0.017 0).Conclusions The study indicates that single nucleotide polymorphisms in PAI-1 may affect breast cancer susceptibility and survival in Chinese Han women.The study may contribute to individualized evaluation of breast cancer risk and patients' prognosis if these data are validated in some other Chinese Han populations.

A retrospective study was conducted on data of 45 patients who underwent endoscopic submucosal dissection (ESD) at Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology from January 2013 to March 2024 and were pathologically confirmed as having early esophageal cancer with postoperative defect ≥3/4 of the esophageal circumference, to evaluate the preventive effect of polyglycolic acid sheet (PGA) combined with oral glucocorticoid for esophageal stenosis after ESD of large early esophageal cancer. Patients were divided into two groups according to teatment: oral glucocorticoid alone group ( n=27) and PGA combined with oral glucocorticoid group ( n=18). The results showed that the postoperative esophageal stenosis rate was significantly lower in the PGA combined with oral glucocorticoid group compared with the oral glucocorticoid alone group [44.4% (8/18) VS 74.1% (20/27), χ2=4.034, P=0.043]. For cases of non-circumferential esophageal defects, only 1 case [9.1% (1/11)] experienced postoperative esophageal stenosis in the PGA combined with oral glucocorticoid group, while 10 cases [62.5% (10/16)] in the oral glucocorticoid alone group. During follow-up, no serious complications related to glucocorticoid use or adverse events related to PGA use were observed. These findings suggest that PGA combined with oral glucocorticoid is safe and effective for preventing esophageal stenosis after ESD for large early esophageal cancer and may be more effective than using only oral glucocorticoid.