1.Construction, reliability and validity of a professional identity questionnaire for psychiatrists
Pei HE ; Xintong DENG ; Longtao HE ; Rui YANG ; Junmei WANG ; Fangyu DENG
Sichuan Mental Health 2024;37(2):144-149
BackgroundDomestic researches on the professional identity of psychiatrists are still relatively rare up to now, and effective evaluation tools remain inadequate. ObjectiveTo construct a professional identity questionnaire for psychiatrists with high reliability and validity, so as to provide an effective tool for the evaluation of professional identity of psychiatrists. MethodsA self-designed questionnaire titled "Professional Identity Questionnaire" was compiled. After preliminary screening of items and expert consultation, an initial questionnaire was formed and administered to a sample of 400 psychiatrists, and the final questionnaire was assessed by item analysis, reliability analysis and validity analysis. ResultsThe Cronbach's α coefficient was 0.930 for the final questionnaire, and ranged from 0.817 to 0.920 for each dimension. The value of KMO was 0.904 (χ2=2 931.652, P<0.01). The cumulative variance contribution rate of factors extracted from exploratory factor analysis was 68.306%. Confirmatory factor analysis showed that the integrity of fit indexes were χ2/df=1.234, GFI=0.905, NFI=0.916, RMSEA=0.036, CFI=0.983, IFI=0.983. The final version of the professional identity questionnaire for psychiatrists consisted of 23 items relating to 4 dimensions, namely, professional cognition, professional behavior, professional emotion and professional values. ConclusionThe professional identity questionnaire for psychiatrists has good reliability and validity, and can be used as an effective tool to evaluate the professional identity of psychiatrists. [Funded by Foundation of Sichuan Research Center of Applied Psychology of Chengdu Medical College (number, CSXL-22304); Science and Technology Guiding Plan Project of Guangyuan City (number, 22ZDYF0072)]
2.Clinical and imaging features of phosphaturic mesenchymal tumors.
Cheng CHANG ; Aihong YU ; Yuhua YOU ; Xiaoxin PENG ; Xiaoguang CHENG ; Xintong LI ; Wei LIANG ; Lihua GONG ; Wei DENG
Chinese Medical Journal 2023;136(3):351-353
3.Discovery and identification of EIF2AK2 as a direct key target of berberine for anti-inflammatory effects.
Wei WEI ; Qingxuan ZENG ; Yan WANG ; Xixi GUO ; Tianyun FAN ; Yinghong LI ; Hongbin DENG ; Liping ZHAO ; Xintong ZHANG ; Yonghua LIU ; Yulong SHI ; Jingyang ZHU ; Xican MA ; Yanxiang WANG ; Jiandong JIANG ; Danqing SONG
Acta Pharmaceutica Sinica B 2023;13(5):2138-2151
Using chemoproteomic techniques, we first identified EIF2AK2, eEF1A1, PRDX3 and VPS4B as direct targets of berberine (BBR) for its synergistically anti-inflammatory effects. Of them, BBR has the strongest affinity with EIF2AK2 via two ionic bonds, and regulates several key inflammatory pathways through EIF2AK2, indicating the dominant role of EIF2AK2. Also, BBR could subtly inhibit the dimerization of EIF2AK2, rather than its enzyme activity, to selectively modulate its downstream pathways including JNK, NF-κB, AKT and NLRP3, with an advantage of good safety profile. In EIF2AK2 gene knockdown mice, the inhibitory IL-1β, IL-6, IL-18 and TNF-α secretion of BBR was obviously attenuated, confirming an EIF2AK2-dependent anti-inflammatory efficacy. The results highlight the BBR's network mechanism on anti-inflammatory effects in which EIF2AK2 is a key target, and inhibition of EIF2AK2 dimerization has a potential to be a therapeutic strategy against inflammation-related disorders.