Objective To explore causes of complications after endovenous laser treatment (EVLT) , for patients with superficial varicosities of the lower extremities. Methods In this study, 207 patients with a total of 268 lower limbs were treated by EVLT combined with surgical procedures. According to CEAP classification, there were C2 in 268 limbs, C3 in 56 limbs, C4 in 101, C5 in 23 and C6 in 18. Simple EVLT was performed for 25 limbs in 21 patients; EVLT combined with external banding valvuloplasty of superficial femoral vein for 23 limbs in 23 patients; EVLT combined with ligation of great saphenous vein (CSV) and denudation of superficial varicosities for 220 limbs in 163 patients. Results An average of 10?5 (3-24) months of follow up was achieved in 175 cases. Early complications (within the first 2-weeks after operation) included bruising or ecchymosis (178 cases, 66.42%) and skin burning (56, 20.90% ). Three month complication included painful induration or trabs (235 cases, 87. 69% ) as well as thrombophlebitis (28, 10. 45% ). Long-term complication (after 3 months) included skin numbness or pain at medial knee or ankle region (15,5. 60% ). Recanalization of CSV occurred in 4( 1. 49% ) treated limbs. Residual or reoccurred varicose veins were found in 13(4. 85% ) cases. Movement related swelling appeared in 6(2. 24% ) lower limbs. Conclusion EVLT is effective for patients with superficial varicosities of the lower extremities. Minor complications most often subsided within 3 months. Strict surgical protocol and high ligation of the GSV could prevent local recurrence of the varicose veins and the recanalization of GSV.

Objective: To evaluate the similarity of Cocculus orbiculatus root and stem by high performance liquid chromatography (HPLC) fingerprint, and to provide a reference for evaluation and clinical application of C. orbiculatus root and stem. Methods: HPLC was applied to chromatographic separation of C. orbiculatus root and stem in different seasons from 14 areas of Guizhou province. The chromatography conditions were: Agilent ODS C18 (250 mm × 4.6 mm, 5 μm) column, mobile phase of CAN 0.1% H3PO4, running time of 95 min, flow speed of 1.0 mL/min, detection wavelength of 254 nm, and column temperature of 30 ℃. The data were processed by Chinese medicine chromatographic fingerprint similarity evaluation system 2.0. Different C. orbiculatus root and stem were subjected to similarity analysis. Results: The chromatography fingerprint of C. orbiculatus root and stem was established and eight common characteristic peaks were demonstrated. The similarities of the chromatography fingerprint were between 0.985-0.998. Conclusion: The chemical compositions of C. orbiculatus root and stem is similar, but the active substances quantity of C. orbiculatus from different regions and seasons are different.

Objective: To promote the comprehensive utilization in the root barks and stem barks of Toddalia asiatica and to establish an HPLC method for the determination of the fingerprints for the root barks and stem barks of T. asiatica. Meanwhile, the similarity was compared by similarity evaluation software based on the fingerprints. Methods: HPLC analysis was performed on C18 (250 mm × 4.6 mm, 5 μm) with acetonitrile and 0.1% aqueous solution of phosphoric acid as mobile phase in gradient mode at the wavelength of 269 nm, and the flow rate was 1 mL/min. The similarity was calculated by comparison on the fingerprints derived from 10 bathes of T. asiatica root barks and 10 bathes of T. asiatica stem barks, all the samples were collected from the different areas in different seasons. Results: The mutualmode of HPLC fingerprints was set up and the similar degrees to the crude drugs of different sources were compared. The content ratio of active ingredients is different from samples in different areas and seasons. Conclusion: The fingerprint similarity is greater than 0.9 between T. asiatica root barks and T. asiatica stem barks, so the compositions are highly similar.

Objective To find the key miRNA that relative to peritoneal fibrosis associated with peritoneal dialysis (PD) by microarray technology,and verify its expression in vitro and in vivo.Methods The peritoneal fibrosis mouse model associated with PD were established by intraperitoneal injection of lipopolysaccharide (LPS)+ 4.25％ peritoneal dialysate.The expression of miRNA was detected by microarray in peritoneal tissues.The expression of miRNA profiles between fibrotic and normal peritoneal tissues was compared.The differentially expressed miRNA (miR-200a) was validated by real-time PCR in lager sample size cohorts.The expressions of miR-200a were also detected in the epithelial-mesenchymal transition (EMT) process of human peritoneal mesothelium cells.Results In mice model of PD,peritoneal tissue was markedly thickened and with a massive extracellular matrix accumulation.In contrast with control,the expression level of epithelial marker E-cadherin was significantly decreased,α-SMA,Col-Ⅰ and FN were remarkably increased (P ＜ 0.05).By miRNA microarray analysis,miR-200a was significantly down-regulated (3.31 folds change,P ＜ 0.05) in fibrotic peritoneal tissues.The down-regulated expression level of miR-200a was also validated by realtime PCR in larger cohorts (P ＜ 0.05).Then,the expression level of miR-200a was detected in the EMT process of human peritoneal mesothelium cells.During the process of TGF-β1 induced EMT,miR -200a was significantly down-regulated compared with the control (P ＜ 0.05).Conclusions Downregulated expression of miR-200a was observed both during peritoneal fibrosis and TGF-β 1 induced EMT in vivo and in vitro,suggesting that miR-200a may be involved in the peritoneum fibrosis by regulating the target genes of EMT.

ObjectiveTo compare the effects of Qishen Fukang capsules and Fluoxetine on cognitive function in first-episode depressions.MethodsBetween December 2011 and January 2012,63 depressed patients with first-episode from Center for Mental Disease Control and Prevention of Third Hospital of PLA were enrolled,and were divided randomly into the Qishen Fukang capsules-treated group ( Chinese medicine group,CMG,31 cases) and fluoxetine treated group (Western medicine group,WMG,32 cases; Jiangsu changzhou pharmaceutical Co.,LTD production) by the method of the random number table.The therapeutic dose was oral 0.2～0.6 g (three times/d) for each CMG patient,and morning oral 20～40 (20 ± 5) mg/d for each WMG patient.Meanwhile,each patient was given the short - term small dosesof benzodiazepine drugs,but no other antidepressants.Each patient and control was assessed with 17-item Hamilton Depression Scale (HAMD).A total of 32 healthy subjects were involved as control group.Each patient was measured with evoked event-related potential P300 change before and after 6 weeks treatment.And compared the effects of Qishen Fukang capsules and Fluoxetine on cognitive function in first-episode depressions before and after treatment.Results(1) Compared with prior-treatment [ CMG ( 29.1 ± 5.1 )score vs WMG(29.0 ± 4.5)score],the HAMD scores of post-treatment [ CMG( 10.1 ± 3.2) score vs WMG (12.3 ± 3.4) score] were decreased significantly ( P＜0.05).The HAMD scores in CMG were significantly lower than the WMG( P＜0.05).The HAMD reductive rate in CMG was significantly higher than the WMG [ (65.6 ± 2.1 ) ％ vs (57.9 ± 3.2 ) ％,P ＜ 0.05 ].(2)compared with prior-treatment,the latency periods of post-treatment were shortened[ P2 (152.8 ± 54.1)ms vs (208.9 ± 57.6)ms,(174.5 ±63.2)ms vs (207.3 ± 55.8) ms;N2(208.7 ± 57.9)ms vs (273.4 ± 62.0) ms,(239.2 ± 59.2) ms vs (275.6 ± 60.8)ms; P3(319.1 ±60.2)ms vs (396.3 ± 66.3)ms,(315.6 ± 61.1)ms vs (394.7 ±55.6)ms ],while the amplitudes were prolonged [ P2 (7.8 ± 1.7 ) μV vs ( 3.3 ± 1.2 ) μV,( 7.0 ± 1.4 ) μV vs　(3.4±1.4)μV; N2 (3.6±1.4)μV vs (1,0±0.7)μV,(2.4±1.3)μV vs (1.2 ± 1.0)μV; P3 (9.6±2.2)μV vs (4.5 ± 1.0)μV,(7.5 ±2.2)μV vs (4.6 ± 1.2)μV] in the CMG and WMG (all　P ＜0.05).Compared with the CMG,the latency periods of F2,and N2 were signiflcantly longer (all P ＜ 0.05 ),and the latency period of p3 was no difference ( P＞0.05 ),and the amplitudes of N2,and P3 were lower ( all P ＜ 0.05 ),and the amplitude of P2 was no difference ( P＞0.05 ) in the post-treatment of WMG.(3)Compared with the controls,the latency periods of P2,N2,and P3 were shortened,while the amplitudes were prolonged in the post-treatment of CMG and WMG ( all P ＜ 0.05 ).The latency periods and amplitudes of P2,N2,and P3 did not show any difference in the CMG after treatment ( all P ＞ 0.05).The latency period of P2,and N2 were still significantly longer (all＜0.05) and the latency period of P3 was no difference ( P＞0.05) while the amplitude of P2,N2,and P3 were still significantly lower (all P ＜ 0.05) in the post-treatment of WMG.ConclusionsQishen Fukang capsules and Fluoxetine can improve significantly cognitive function in first-episode depressions.Qishen Fukang capsules is superior to fluoxetine on improving the early preparation efficiency of information processing.

Adolescent ; Adult ; Female ; Humans ; Male ; Occupational Diseases ; etiology ; prevention & control ; Occupational Exposure ; analysis ; prevention & control ; Trichloroethylene ; poisoning ; Young Adult

The aim of this study is to synthesize the ordered mesoporous silica (OMS) as drug carrier to improve release property of insoluble drug and investigate the dissolution profile of insoluble drug from the porous carrier. The OMS was obtained by using cetyltrimethyl ammonium bromide as the template and resveratrol was selected as the model drug. The resveratrol-loaded OMS (Res-OMS) were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), N2 adsorption-desorption, X-ray diffraction (XRD) and FT-IR spectroscopy. In vitro drug release behavior was also investigated. It was found that the synthesized OMS showed a large surface area, a narrow pore size distribution and an important mesoporosity associated to hexagonally organized channels. Compared with physical mixture and crystalline powder, resveratrol was in amorphous or molecular form after loading into OMS. The release rate ofresveratrol from drug-loaded OMS was significantly increased suggesting the great potential application of OMS for the formulation of poorly soluble drugs.

The σC gene of ARV S1133 was designed to amplify by reverse transcription chain reaction(RTPCR).The σC gene was inserted into the vector pMD19-T,identified by PCR method and restriction enzyme,and sequenced.It showed that the insert cloned gene fragment was the σC gene of ARV.Then the gene was inserted intc the pET32a(+) and indicated that fusion expression vector pET32a-σC was constructed.The recombinant fusion protein was highly expressed in E.coli BL21 induced by 1.0 mmol/L IPTG for 5 hours in the form of inclusion bodies.The weight of recombinant fusion protein molecular is 54 000.Western-blot with ARV antibodies against the fusion protein showed the recombinant protein has a favourable reactivity.

Objective To evaluate the effect of oxycodone on migration of human colon cancer cells and the role of μ and κ receptors.Methods The human colon cancer HCT116 cells at the logarithmic growth phase were seeded in 24-well or in 6-well plates at a density of 1 × 106 cells/mnl (0.5 ml/well or 2 ml/well,144 wells in total).The cells were divided into 6 groups (n=24 each) using a random number table:control group (group C),1,5 and 10 μmol/L oxycodone groups (group O1,group O2 and group O3),oxycodone plus μ receptor antagonist CTOP group (group O2+CTOP) and oxycodone plus κ receptor antagonist nor-binaltorphimine group (group O2+BNI).The cells were incubated for 24 h with oxycodone 1,5 and 10 μmol/L in O1,O2 and O3 groups,respectively.The cells were incubated for 24 h with 5 μmol/L oxycodone plus 20 μmol/L CTOP and 5 μmol/L oxycodone plus nor-binahorphimin 20 μmol/L in O2+CTOP and O2+BNI groups,respectively.The invaded and migrated cells were counted,and the levels of Ras homolog gene family member A (RhoA),Rho-associated protein kinase 1 (ROCK1),matrix metalloproteinase-2 (MMP-2) and MMP-9 were detected.Results Compared with group C,the number of invaded and migrated cells was gradually decreased,and the levels of RhoA,ROCK1,MMP-2 and MMP9 were gradually decreased in O1,O2 and O3 groups (P＜0.05),and no significant change was found in the parameters mentioned above in group O2+BNI (P＞0.05).Compared with group O2,the number of invaded and migrated cells was significantly increased,and the levels of RhoA,ROCK1,MMP-2 and MMP9 were increased in group O2 + BNI (P＜0.05),and no significant change was found in the parameters mentioned above in group O2+CTOP (P＞0.05).Conclusion Oxyc odone can inhibit the migration of human colon cancer cells,and the mechanism is totally related to inhibition of RhoA/ROCKl signaling pathway activation after activating κ receptors,but not related to μ receptors.

A total of 72 patients with first-episode mild-and-moderate depression took Qishen Fukang Capsules.The change of event-related potential P3 was determined before and after treatment for 6,12 and 24 weeks.And another 70 healthy controls received the same tests simultaneously.Compared with before treatment,HAMD scores after treatment were lower in the patient group [(29.1 ± 5.1) score vs.(10.4 ±4.1) score].P3 latencies were shorter and amplitudes became higher after treatment (P ＜0.01).And the difference of before and after treatment for 6 weeks was larger than the difference of 12 weeks and after treatment for 6 weeks (P ＜ 0.01).The curative effect is definite.And the clinical symptoms and brain evoked potential index have improved in patients with first-episode mild-and-moderate depression after medication.