With the development of modem tumor diagnostic techniques and therapies, the survival rate of malignant tumor has been remarkably increased. With the increase in survival rate of cancer-beating patients, the chance of tumor metastasis is elevated. Minimally invasive treatment provides a new strategy to the therapy of spinal metastatic carcinoma. Its benefits include smaller surgical wound,, higher efficacy and fewer complications, and thus have a broad application prospects in the field of spinal metastatic tumors. In this article, a general re-view is given on minimally invasive treatments of spinal metastatic carcinoma, as well as the latest progress in the field. It was proposed that the united application of the treatment might be a promising hot spot of tumor therapy.

Poly lactic-co-glycolic acid (PLGA) has been attracting increasing attention in the field of bone tissue engineering because it is a biodegradable polymer with excellent biocompatibility, controllable aperture and porous rate, and a degradation rate which is suitable for bone regeneration, and moreover a suitable carrier for the sustained release of growth factors. This article reviewed the recent research progress in PLGA as a material for controlled drug delivery with emphasis on how to make the scaffold more suitable for the regeneration of the bone and which is the better way to apply sustained release of growth factors embedded in the material in bone tissue engineering. It pointed out that PLGA carrying different growth factors will become the focal point in the field of bone tissue-engineering.

The content of total alkaloid in the seed of Peganum harmala L. was determined by bro-mophenol blue colorimetry. It could be considered that this is a specific, quick and simple method for thedetermination of total alkaloid in the seed of P. harmala.

Objective To assess the feasibility of using biodegradable poly (lactic-co-glycolic acid) (PLGA) scaffold with controlled release of rhBMP-2 and its effect on the osteogenic activity of MSCs in vitro. Methods The PLGA scaffolds with rhBMP-2 sustained release system were made by combination of porogen-leaching and freeze-drying. The drug release kinetics was measured by enzyme-linked immunosorbent assay (ELISA) in vitro. MSCs were isolated from the bone marrow of human and cultured for 3 passages. Then, MSCs were seeded onto PLGA scaffolds. PLGA scaffolds without rhBMP-2 were used as experimental controls . Scanning electron microscopy(SEM) was used to observe the morphology of MSCs. The cell proliferation was determined by MTT assay. Results The rhBMP-2 was encapsulated into PLGA scaffolds and it was found to be continuously released from the scaffolds. The scaffolds have evident enhancing effect on ectopia osteogenesis. Conclusion PLGA scaffold containing rhBMP-2 is a new promising tissue engineering scaffold.

The materal-neonatal safety and materal stress response were evaluated during intratheeal anesthesia. Method: Forty-two parturients(ASA grade 1 )were randomly divided into two groups: spinal group (n=21), epidural group (n=21). 3 ml of 0.5% hyperbaric bupivacaine was given in spinal group; 12-15ml of 0.75 % bupivacaine was administrated in epidural group. Materal arterial samples were drawn before anesthesia,after anesthesia induction, after delivery and at the end of surgery; umbilieal venous samples were taken for measurements of blood catecholamine, sugar and lactate concentrations. Result; Compared with those before intrathecal blockade, plasma levels of catecholamine of two groups were significantly decreased in different periods during anesthesia (P0.05); there were not any significant differences between the two groups in neonate Apgar scores. Conclusion:Both of two techniques can inhibit stress response of parturients to cesarean sections and have no adverse effects to neonates when applied in proper management.

Objective To analysis the DNA sequence of HEV ORF3 cDNA and express in HepG2 cell .Methods Recombinant vector carrying HEV ORF3 cDNA was constructed and identified by DNA sequencing .Expression of recombinant vector in HepG2 cell was determined by immunofluorescence technique .Results The recombinant vector was constructed and expressed in HepG2 cell successfully .Conclusion The research laid experimental foundation for exploring the biological function of ORF3 protein in the pathogenesis of viral hepatitis E .

Objective To compare the clinical results of two different anterior cervical surgical treatment for multi-segmental cervical spondylotic myelopathy (≥3 segments).Methods Twenty-three patients with segmental cervical spondylotic myelopathy,10 cases were treated with anterior cervical discectomy and fusion (ACDF) as ACDF group,13 cases were treated with anterior cervical corpectomy and fusion (ACCF) as ACCF group.The operation time,operative blood loss,JOA scores,neurological improvement rate and the variable of the D value were compared.Results The operation time and operative blood loss in ACDF group was significantly lower than that in ACCF group [(130.0 ±31.5) min vs.(150.0 ±42.5) min,(150.0 ± 120.8) ml vs.(310.0 ± 320.8) ml,P ＜ 0.05].The variable of the D value in ACDF group was significantly higher than that in ACCF group [(3.1 ± 1.4) mm vs.(2.3 ± 0.9) mm,P ＜ 0.05].There was no statistically significant difference in JOA scores,neurological improvement rate between ACDF group and ACCF group.Two cases of cerebrospinal fluid leakage in ACDF group,the oppression and drainage recovery after treatment.One case of 14 d after fistula complications in ACCF group,after patching were cured; 2 cases of titanium mesh shift,follow the fusion.Conclusions Both methods attain good clinical results.ACDF combined with ACCF treatment of multi-segmental cervical spondylotic myelopathy,with shorter operation time,relatively less blood loss,and better restoration of cervical sagittal alignment.

BACKGROUND: Extract of astragalus mongholicus has good effects on antioxidization and oxygen free radical scavenge. Bone marrow mesenchymal stem cells (BMSCs) have multiple directional differentiation potential and superiority of autologous transplantation. This can provide a new way for treating neurodegenerative diseases.OBJECTIVE: To explore the differentiation of BMSCs of rats following astragalus mongholicus induction.DESIGN: A cytological in vitro study.MATERIALS: One clean male rats aged 6 weeks were purchased from Experimental Animal Center of Chinese Academy of Medical Sciences. Astragalus mongholicus injection (lot number 060105) were obtained from Dali Pharmaceutical Co., Ltd.METHODS: Rat BMSCs were in vitro isolated, cultured and purified by the whole bone marrow method. At the fourth passage, BMSCs at a density of 4×10<'8>/L were incubated in 12-well plate with a coverslip. After 80% 90% cells were confluent, the medium was changed. BMSCs were then incubated in DMEM, supplemented with 200 g/L astragalus mongholicus injection and 15% fetal bovine serum, for 6 days. BMSCs in the control group were not treated with astragalus mongholicus injection.MAIN OUTCOME MEASURES: Morphological changes in BMSCs were observed under the inverted microscope before and after induct.ion. Expression of specific markers was determined by immunocytochemical staining following induction.RESULTS: Primary cultured BMSCs were round. At the fourth passage, BMSCs were spindle. BMSCs after induction showed processes, which became more with prolonged time. Some processes were branch-shaped, forming a network structure. Results of immunocytochemical staining demonstrated that many cells were positive for nestin, neuron specific enolase and glial fibrillary acidic protein on day 3, and abundant cells were positive for microtubule-associated protein-2 on day 6.CONCLUSION: Astragalus mongholicus induces differentiation of BMSCs into neural stem cells, and then promotes the differentiation into neurons or glial cells, and makes differentiated cells mature and aging.

Objective To compare the effects of propofol and ketamine on long-term memory and the expression of brain N-methylgroup-D-aspartate receptor 2B(NMDAR2B) and Gamma-aminobutyric acid receptor 1(GABAR1) in aged rats, and preliminary investigate the relation between the long-term memory and expression of neurotransmitter receptors in different cerebral areas. Methods The aged male rats were randomly divided into control group,propofol group and ketamine group. Morris water maze training was performed in all the rats of three groups for 5 days. On the 6th day, intraperitoneal injection of 50 mg?mL-1 propofol was administrated in propofol group,80 mg?mL-1 ketamine was intraperitoneally injected in ketamine group,and blank control group was given the same dose of saline.Seven days after the administration,space exploration experiment and navigation experiment test were performed to test the impact on the learning and memory ability of rats. After that, the expression levels of GABAR1 and NMDAR2B in temporal lobe and hippocampal CA1 region of the rat brain were detected by immunofluorescence and FISH technique. Results The results of Morris water maze showed there was no significant difference between propofol group (9.49±1.24) s and blank control group (8.82±2.22) s.There was statistically significant difference between ketamine group (12.04±2.67) s and blank control group (P<0.05),with longer latency time and less number of times of passing through target as compared with blank control group.By using immunohistochemistry and FISH technique,the expression of GABAR1 in temporal lobe and hippocampal CA1 region of the rat brain was not significantly different between propofol group and blank control group,but it was significantly up-regulated in ketamine group as compared with blank control group ( P<0.05) . The expression of NMDAR2B in temporal lobe and hippocampal CA1 region of the rat brain was not significantly different between propofol group and blank control group,but it was significantly down-regulated in ketamine group as compared with blank control group (P<0.05). Conclusion Propofol anesthesia alone had no effect on long-term learning and memory,but ketamine anesthesia can result in long-term learning and memory impairment. The mechanism may be related with down-regulation of the expression of NMDAR2B receptor and up-regulation of GABAR1 not only in CA1 region hippocampus,but also in temporal lobe.

Objective To observe the change of learning and memory quality and the expression change of GABAR1 and NMDAR2B in aged SD rat cerebral temporal lobe by propofol injection and discuss the influence that may be caused by propofol.Methods Aged male sd rats were randomly divided into three groups,each group of 20.Propofol intraperitoneal injection was implemented to groups P1 and P7 according 50 mg/kg and maintained anesthesia for 3 hours.The same dose of emulsion was given to control group. Morris water maze test was done in the first days and the seventh days after intraperitoneal injection.Then the left temporal lobe was gathered and the mRNA transcription and protein expression of GABAR1 and NMDAR2B were detected by FISH and Immunofluorescence technique.Results Compared with control group,the latent time of group P1 was significantly prolonged and the numbers of passing through the target frequency were decreased significantly (P <0.05).Group P7 had no significant differences.The mRNA transcription and protein expression of GABAR1 in group P1 were increased significantly and the mRNA transcription and protein expression of NMDAR2B in group P1 were decreased significantly (P <0.05 ). Group P7 had no significant differences.Conclusion The short-term memory of brain declined after propofol was used.This may be related to the function of temporal lobe.The mechanism may be related to the up regulation of GABAR1 and the down regulation of NMDAR2B simultaneously.