Objective To study the approach to improving the diagnosis and treatment of thyroid cancer(TC). Methods Problems regarding to the diagnosis and treatment of TC had arisen based on the combination of auther's clinical experience and recent literatures review. Results and Conclusions (1) In the diagnosis of TC, attention should be paid to the following conditions: cystocarcinoma, multicentral cancer, bilateral TC, coexist of benign thyroid diseases with TC, and the microcancer of thyroid etc. (2) Biopsy should be done during and after operation for the thyroid nodule needed to be operated, to avoid misdiagnosis of TC. (3) Except undifferentiated carcinoma, the choice of treatment of TC is surgery. The choice of operative procedure should be according to the pathological type and clinical stage of TC, and patient's condition. (4) Thyroid hormone suppression therapy should be administrated after operation. (5) Posterative radiotherapy can promote the long term survival rate. (6) Reoperation should be performed immediately, if the thyroid nodule is TC and comfired by pathological examination posteratively, and a subtotal thyroidectomy or nodule local resection performed on for that nodule initially, to avoid the residual cancer and cancer recurrence in the residual thyroid tissues.

Objective To evaluate the result of autologous vein graft used as a conduit for repairing the iatrogenic recurrent laryngeal nerve(RLN) deficit.Methods Iatrogenic RLN deficit was repaired by using antogeneous vein graft as a conduit in 17 patients. RLN deficit occurred in right side in 5 cases, in left side in 12. Results The RLN injuries were located within 2 cm below the point of RLN entering throat in 16 cases(94 1%), near the inferior thyroid artery in 1(5 9%). The length of RLN deficit was between 0 5～4 cm. Among the 17 cases, 16(94 1%) were followed up for more than 1 5 years. Of them, normal phonation was achieved in 12 cases(75 0%), significantly improved in 3(18 8%) and slightly improved in 1(6 2%).Among the 15 cases who received indirect or direct laryngoscopy, the affected vocal cord movement entirely recovered in 4 cases(26 7%), partially recovered in 3(20 0%), unchanged in 8(53 3%) Conclusions Autogeneous vein graft is ideal for repairing RLN deficit.

Objective To explore the expression of apoptosis regulating genes C jun and bcl X L after normothermic liver ischemic preconditioning and its clinical significance. Methods 16 cases of liver cancer were randomly divided into ischemic reperfusion(IR) group and ischemic preconditioning (IP) group (8 cases in each). The samples of venous blood were drawn before IR or IP procedure and 30 minute after reperfusion for testing ALT, AST and LDH. Meanwhile, samples of liver tissue were taken for study of hepatocellular apoptosis, expressions of C-jun mRNA、 Bcl-X L mRNA and PCNA and morphologic changes. Results The levels of ALT、 AST、 LDH and AI in IR group were significantly higher than those in IP group (P

Objective To study the influence of pcDNA3.1-RhoC on the expression of endogenous angiogenic factors in HCC cells.Methods The reconstructed plasmid pcDNA3.1-RhoC was transfected into HepG2 cells, and expression of VEGF and bFGF was detected with the RT-PCR and immunohistochemical stain. HepG2 cells transfected with pcDNA3.1-RhoC or pcDNA3.1 were implanted into nude mice to observe the tumor occurrence rate.Results HepG2 cells transfected with pcDNA3.1/RhoC showed higher expression of RhoC . The expression of RhoC enhanced the expression of VEGF and bFGF(P

Objective To investigate Caspase 3 activity in the ischemia reperfusion injury in rats and the protective effect of ischemic preconditioning and its possible mechanism.Methods 30 rats were randomly assigned to ischemia reperfusion (IR) group, ischemic preconditioning(IP) group,sham-operation(S)group.The serum aspartate transaminase (AST),alanine transaminase(ALT),liver caspase 3 activity,and apoptosis index(AI) of hepatocytes were examined in the three groups at 3 hours after repersusion.Results The serum AST,ALT,liver caspase 3 activity and apoptotic hepatocytes were significantly higher in both IP and IR groups than those in S group(P

[Objective]To observe the cerebral activating effects of needling in Yanglingquan(GB34) vs a sham point of healthy volunteers based on the technique of fMRI.[Methods]Sixteen healthy volunteers were randomly divided into GB34 group and sham point group,and received needling in the different points on the right side respectfully.Super nuclear magnetic resonance(1.5T,GE Co.) was used to scan the brain and the obtained experimental data of the regional brain were processed and analyzed by method of Region of Interest(ROI).[Results]There was not any difference between needling in GB34 and the sham point in the cerebral activating rate.While the activating number of needling in the sham point was more than that of needling in GB34 in bilateral parietal lobes and right occipital lobe(P

Objective To explore the management methods for liver injury combined with posthepatic vein injuries in order to make an improvement in corresponding treatment. Methods Twenty-three cases with liver injury combined with posthepatic vein injuries were treated with the following 5 ways: repairing the hepatic vascular blockage step-by-step, pressing directly to exhibit the injured vessels for repair, ligating the injured hepatic veins, suturing the hepatic tissues and packing with gauze. Results Of all, 18 cases (78.3%) were cured and 5 (21.7%) died. Conclusions Rational preoperative emergent resuscitation and correct complex intraoperative management are key to the liver injury combined with posthepatic vein injuries.

Objective To investigate the effects of low molecular weight heparin (LMWH) on severe acute pancreatitis(SAP). Methods Retrospective analysis was made on the clinical features and therapeutic effect on the routine treatment (RT) group in 48 cases and LMWH treatment(LT) group in 31 cases. Results 28 (90.3%) out of 31 cases were cured while 3 (9.7%) died in LT group. 35(72.9%) out of 48 cases were cured whereas 15 (27.1%) died in RT group. The complications occurring in LT group (32.3%,10 patients 18 times ) was significantly lower than that in the RT group (62.5%,30 patients 59 times)(P

Objective To study the diagnosis and treatment of abdominal aortic anurysm-intestinal fistula(AAA-IF). Methods The diagnosis and treatment of one patient with AAA-IF was reported, while a literateres review of AAA-IF was made. Results A 50 years old femal patient abmitted due to upper abdominal pain for two month, and an AAA was found in the explore laparotomy. The patient suddenly occurred severe upper abdominal pain accompanied with shock and massive upper gastrointestinal bleeding 13 days after the operation. The preoperative suspected diagnosis was AAA-IF. An AAA-IF was ound during emergent operation. AAA-IF resection with artifial vessel replacement, resection of the forth portion of duodenal and upper portion of jejunal were performed together. The patient recovered eventurely except high body temperature. After vigovous antibiotics therapy, the patient recovered. Conclusions AAA-IF is a rare disordor, the mortality can be as high as over 30%. The key points of redecing mortality are that the diagnosis and treatment should be in time; postoperative vigorous antibiotics should be given, because the AAA-IF patient tends to occur septic complications due to the communication occurred between the AAA and intestinal tract.

Objective To establish an experimental model for exploring the role of RhoC gene in the invasiveness and metastasis of hepatocellular carcinoma.Method The RhoC gene was digested with restricted enzyme Hind III and XbaI,and direct cloned to pcDNA3.1.The recombinant vector (pcDNA3.1 RhoC) and the vector alone (pcDNA3.1) were transfected into HEPG2 cells with LIPFECTAMINETMReagent.After selected with hygromycin,resistant cloneies was obtained.The transcription and translation of RhoC gene were analysed with the reverse transcription PCR and immunohistochemical stain.Results The recombinant vectort (pc DNA3.1 Rhoc) express steadily in HerpG2 cells.Conclusions The modified tumor cells(HEPG2 RhoC) could be used to study the effect of RhoC protein on the invasiveness and metastasis of hepatocellular carcinoma.