Objective To study the expression of cyclooxygenase-2(COX-2) in pancreatic tissues of severe acute pancreatitis(SAP) in rats,and explore the possible role of COX-2 in the pathogenesis of the SAP. Methods Forty adult male Sprague-Dawley rats were randomly divided into SAP group(n=20) and control group(n=20). At 12h after operation,acites,serum levels of amylase activity,tumor necrosis factor-?(TNF-?),TXB 2 and 6-keto-PGF 1? and the expression of COX-2 in pancreatic tissue were detected.the pathological changes of pancreatic tissues were observed and scored under microscope;the survival rate in 72h and mortality in 24h in SAP and NC group were recorded.Results At 12h after the induction of SAP,the serum amylase activity,TNF-?,TXB 2 and 6-keto-PGF 1? were significantly higher in SAP group than those in NC group(P

Objective To explore the altered expression of circular RNA (circRNA) and mRNA in the mouse cortex in the early phase of subarachnoid hemorrhage (SAH) and possible biological functions of the circRNA in early brain injury (EBI).Methods A total of 18 C57BL/6J male mice were randomly divided into a sham and a SAH group (n=9).SAH models were prepared by endovascular perforation.Total RNAs of brain samples were extracted to construct the cDNA library 24 h after operation.RNA-sequencing (RNA-seq) was carried out by HiSeqTM 2500 User Guide and followed by RT-qPCR for confirmation.Reads were aligned to the mouse transcriptome to obtain expression profiles ofcircRNA and mRNA.Bioinformatic study included GO analysis,KEGG pathway analysis and forecast of targeted miRNA of circRNA.Results A total of 26 circRNA (6 up-regulated and 20 down-regulated) and 804 mRNA (396 up-regulated and 408 down-regulated) were significantly changed.These altered mRNA were mainly related to regulation of neuronal synaptic plasticity,inflammatory and immune response.Bioinformatics showed that some significantly altered circRNA contained binding sites for many miRNA.The RT-qPCR analysis of 4 randomly selected circRNA (circFoxj3,circSetbp1,circArpp21 and circ2010111 I01Rik) confirmed the accuracy of RNA-seq.Conclusions SAH alters the expression of circRNA in mouse cortex and the differentially expressed circRNA may be involved in regulation of EBI following SAH,promising a potential therapeutic target for the diagnosis,treatment and prognosis of SAH.

Cationic PLG nanoparticles and liposome were prepared and used as package molecules to pack up pUC18-CpG. The effects of the packed pUC18-CpG on the cellular and humoral immune responses were detected in the mice that were inoculated with pig paratyphoid vaccine. The results showed that compared with the control, the amount of IgG and the titre of specific antibody were significantly increased in the sera of mice immunized with the CpG plasmid entrapped by cationic PLG nanoparticles; the proliferation and induced IL-2 bioactivity of lymphocytes were significantly enhanced in the spleen of the immunized mice; the stimulatory effect of cationic PLG nanoparticles was similar to or stronger than that of cationic liposome. These indicated that cationic PLG nanoparticle could be employed as an effective package molecule to promote the immunostimulatory effect of pUC18-CpG.
Adjuvants, Immunologic ; pharmacology ; Animals ; Immunoglobulin G ; blood ; Interleukin-2 ; blood ; Liposomes ; Male ; Mice ; Mice, Inbred BALB C ; Nanostructures ; Oligodeoxyribonucleotides ; pharmacology ; Swine ; Typhoid-Paratyphoid Vaccines ; immunology