1.Establishment of an in vitro model of brain-blood barrier
Ying XIE ; Liya YE ; Xiaobin ZHANG ; Xinpu HOU ; Jinning LOU ;
Journal of Peking University(Health Sciences) 2003;0(04):-
Objective: To establish an in vitro model of brain blood barrier (BBB) using cultured mouse brain microvascular endothelial cells (BMVEC). Methods: Mouse BMVEC were seeded on micro pore membrane of gelatin coated cell culture insert and cultured to confluence. The establishment of BBB was preliminary judged by a 4 h water leaking test. The tight junctions between BMVEC were demonstrated by scanning and transmission electron microscope. The transendothelial electrical resistance(TEER) over BMVEC was measured. The permeability of Horseradish peroxidase (HRP) through the BBB was analyzed and the effect of RMP 7 on permeability of the BBB was investigated. Results: The 4 h water leaking test became positive when BMVEC were cultured to confluence. By scanning and transmission electron microscope, the tight junctions were demonstrated on confluent BMVEC. The TEER over BMVEC monolayer increased 3.2 and 7.68 times and the permeability rates for HRP were 13.4% and 6.7% respectively, as compared with sub confluent BMVEC and human umbilical vein endothelial cell monolayer(HUVEC). The HRP permeability rate in the model of BBB increased 2.7 times after treatment with RMP 7. Conclusion: The established in vitro model of BBB has basic characteristics of BBB in vivo , and is suitable for central nervous system (CNS) drug research over BBB.
2."Clinical Research on ""Yin-deficiency with Inner Heat"" Acne Treatment Using Acupuncture and Moxibustion"
Xinpu ZHANG ; Yanan TONG ; Dan XUE ; Min LI ; Jieying FU
World Science and Technology-Modernization of Traditional Chinese Medicine 2013;(6):1388-1393
This study was aimed to evaluate the clinical effect of 66 yin-deficiency acne cases treated with body constitution adjustment using acupuncture and moxibustion. In this research, 66 acne patients with the body con-stitution of yin-deficiency were selected . These patients were randomly divided into two groups ( 33 every group ) . The acupuncture treatment group was treated by acupuncture and local treatment. And moxibustion treatment group was treated by indirect moxibustion and local treatment. The treatment was given twice a week. And the improvement condition of acne skin lesions and yin-deficiency body constitution were evaluated after 12-week treatment. The results showed that certain curative effects were received in both groups. However, the moxibustion treatment group had higher curative effect compared with the acupuncture treatment group. There were significant statistical differences between two groups ( P < 0 . 05 ) , which meant moxibustion with local treatment had a higher curative effect than acupuncture with local treatment to acne. In the aspect of skin lesion improvement, according to the statistics, the moxibustion treatment group were statistically significant compared with acupuncture group (P< 0.05), which meant more obvious improvement of skin in the moxibustion treatment group. In the aspect of body constitution, the body constitution scores in both groups were significantly declined and the moxibustion treatment group was more obvious. There was statistical significance between two groups (P < 0.05), which meant patients in the moxibustion treatment group obtained a more obvious body constitution improvement. After the treatment, follow-up was given to all patients for 4 weeks. The results showed that both the acne skin lesion con-dition and yin-deficiency body constitution score were decreased and not achieve the recurrence diagnostic stan-dard. It showed that acupuncture and moxibustion are able to activate the self-regulation and disease resistibility of the body. It was concluded that acupuncture and moxibustion not only improve skin lesion of acne patients with the body constitution of yin-deficiency with inner heat , but also regulate yin-deficiency body constitution . Moxibustion had higher efficacy in the treatment of yin-deficiency with inner heat acne than acupuncture.
3.Study on relationship between serum IDO level and regulatory T cells in patients with ulcerative colitis
International Journal of Laboratory Medicine 2018;39(1):21-24
Objective To study the relationship between serum indoleamine 2 ,3-dioxygenase(IDO) and the level of regulatory T cells(Treg) in the patients with ulcerative colitis (UC) .Methods Seventy cases of UC treated in the Hainan Provincial People′s Hospital from January 2015 to February 2017 were selected as the observation group .In the same period ,70 healthy volunteers undergoing physical examination were selected as the control group .The levels of serum IDO and Treg were compared between the two groups ,the expression levels of serum IDO and Treg in of the patients with different disease activity periods and different severity de-grees of UC were observed ,and the correlation between the level of serum IDO and the Treg expression level in the patients with UC was analyzed .Results The serum IDO level in the observation group was significantly increased compared with that in the control group ,while the Treg expression level decreased which compared with the control group ,the difference was statistically significant (P<0 .05) .The serum IDO level in the pa-tients with activity period of the observation group was significantly increased compared with that in the pa-tients with remission period ,but the Treg expression level was decreased compared with that in the patients with remission period ,the difference was significant (P<0 .05) .The serum IDO and Treg expression levels had statistical difference among different disease degrees in the patients with active UC (P<0 .05) .The serum IDO level in the moderate and severe groups increased which compared with that in the mild group ,while the Treg expression level was significantly decreased compared with that in the mild group ,the difference was sig-nificant (P<0 .05) .The level of serum IDO in the severe group was significantly higher than that in the mod-erate group ,while the Treg expression level was significantly lower than that in the moderate group ,the difference was statistically significant ( P < 0 .05 ) .Conducting the correlation analysis according to the Spearman method found that there was a negative correlation between the serum IDO level and the Treg ex-pression level in the patients with UC (r= -0 .798 ,P=0 .000) .Conclusion The level of serum IDO in the pa-tients with UC is negatively correlated with the Treg expression level ,which jointly influence the occurrence and development of UC .
4.One-stage hybrid surgery for cerebrovascular diseases
Xinpu CHEN ; Xianzhi LIU ; Guang ZHAI ; Peichao ZHAO ; Zhifeng ZHANG ; Jianjin BAO ; Fengjiang ZHANG
Chinese Journal of Neuromedicine 2014;13(7):741-743
Objective To explore the preliminary experience of a one-stage hybrid operating room (OR) in cerebrovascular surgery.Methods A total of 23 patients [9 male,mean age:(40.0±11.2) years] underwent one-stage hybrid approach in a hybrid OR from September 2012 to December 2013,were chosen in our study.Craniotomy and percutaneous intervention of these patients were performed in a single session.Their clinical data were retrospectively analyzed.Results Thirty-one times of digital subtraction angiography (DSA) was performed in all patients,and 15 patients were diagnosed as having intracranial aneurysms and 8 arteriovenous malformations (AVMs).In one patient,a reposition of the clip was needed due to neck remnant after clipping.Residual nidus resection was done in 2 patients with AVMs.Temporary balloon occlusion happened in 5 patients,parent artery occlusion in 3,and 8 accepted emergency surgery under DSA confirming cerebrovascular diseases and removing hematoma.No procedural complications was observed.Conclusion A combined endovascular and surgical approach conducted in a one-stage hybrid OR provides a new strategy for the treatment of complex and emergency cerebrovascular diseases.
5.Hospitals' responsibility in response to the threat of infectious disease outbreak in the context of the coronavirus disease 2019 (COVID-19) pandemic: implications for low- and middle-income countries
Ji ZHANG ; Xinpu LU ; Yinzi JIN ; Zhi-Jie ZHENG
Global Health Journal 2020;4(4):113-117
The WHO declared the coronavirus disease 2019 (COVID-19) outbreak as a public health emergency of international concern on January 30, 2020, and then a pandemic on March 11, 2020. COVID-19 affected over 200 countries and territories worldwide, with 25,541,380 confirmed cases and 852,000 deaths associated with COVID-19 globally, as of September 1, 2020. 1While facing such a public health emergency, hospitals were on the front line to deliver health care and psychological services. The early detection, diagnosis, reporting, isolation, and clinical management of patients during a public health emergency required the extensive involvement of hospitals in all aspects. The response capacity of hospitals directly determined the outcomes of the prevention and control of an outbreak. The COVID-19 pandemic has affected almost all nations and territories regardless of their development level or geographic location, although suitable risk mitigation measures differ between developing and developed countries. In low- and middle-income countries (LMICs), the consequences of the pandemic could be more complicated because incidence and mortality might be associated more with a fragile health care system and shortage of related resources. 2-3 As evidenced by the situation in Bangladesh, India, Kenya, South Africa, and other LMICs, socioeconomic status (SES) disparity was a major factor in the spread of disease, potentially leading to alarmingly insufficient preparedness and responses in dealing with the COVID-19 pandemic. 4 Conversely, the pandemic might also bring more unpredictable socioeconomic and long-term impacts in LMICs, and those with lower SES fare worse in these situations. This review aimed to summarize the responsibilities of and measures taken by hospitals in combatting the COVID-19 outbreak. Our findings are hoped to provide experiences, as well as lessons and potential implications for LMICs.