Objective:To study the correlation between C-myc express io n and clinicopathological characteristics of oromaxillofacial sarcoma. M ethods:C-myc expression was determined by SP method in 37 cases of sarc oma and 14 benign mesenchymal tumors in oral and maxillofacial region. R esults:C-myc expression was detectd in 22 cases (40.54%) of sarcoma and in none of the benign tumor. In sarcoma of gradeⅠ, Ⅱand Ⅲ, the positive r ates of C-myc expression were 16.70%, 30% and 66.67% respectively, and a statis tical significance was only observed in C-myc expression between gradeⅠand Ⅲ( P

0.05), but were highly significant at doses of 100 ?mol/L or above (P

Objective Explore the value of APM histochemical stain technique in the diagnosis of amniotic fluid embolism(AFE).Method The pulmonary tissues taken from autopsies performed in 1988～2001 including 19 AFE cases and 3 babies died of amniotic fluid aspiratory pneumonia(positive control),10 women in labor died of other causes(negative control) were sectioned again and stained by APM method and HE method. Comparative statistical research was carried out on findings from the 2 staining methods. Results By APM stain,amniotic fluid elements could be found in all AFE cases and positive control cases as well as some negative control cases.In addition,we found 1 positive case neglected and 1 false negative case of AFE retrospectively.Statistical comparison revealed that APM stain can elevate the detection rates both for keratinized epithelium and mucus.Conclusion APM staining provided possibility of detecting some amniotic fluid elements that was not distinguished by HE stain. The APM stain is helpful and can be used for AFE diagnosis in routine histological examination.

Objective To study the protective effect of resveratrol on endothelial cells injury induced by oxidized low-density lipoprotein (oxLDL) and its protective mechanism. Methods The injured model was established by endothelial cell treated with oxLDL. In the experiment, endothelial cell were cultured in vitro and divided into seven groups which were: control group; solvent (DMSO) group, oxLDL group, quercetin plus oxLDL group, Reseveratrol-L (0.5 mol/L), M(5 mol/L), H(50 mol/L) plus oxLDL group respectively. Endothelial cells were incubated with 200?g/ml oxLDL for 18 hours in the absence or presence of various concentrations of resveratrol and quercetin. The apoptotic index was detected by flow cytometric. Meanwhile, the content of Malondialdehyde (MDA), and the activity of SOD, GSHPX in the culture medium and homogenate of endothelial cells were measured. Results Incubation of endothelial cells with oxLDL for 18 h elicited a significant increase in the percent of apoptotic endothelial cells, in control group, the percent of apoptotic cells is 7.6?0.6, however in oxLDL group, it increased to 41.4?2.3, furthermore, oxLDL induced the increase of the contents of MDA, and accompanied by the decrease in the activity of SOD, GSHPX in the endothelial cells, the difference of was significant as compared with control group. Pretreatment of resveratrol with 0.5～50 mol/L decreased these oxLDL-induced changes, in Reseveratrol with 50 mol/L group, the percent of apoptotic cells decreased to 13.0?1.5, the difference of was significant as compared with oxLDL group (P

砄bjective:To study the characteristics of proliferation and differentiation of malignant ameloblastoma.Methods:The expression of PCNA,Ki 67, CK19 and CK10 and CK13 proteins in 17 cases of malignant ameloblastoma was detected immunohistochemically by SP method. Results: The proliferating indices of PCNA and Ki 67 were significantly higher in malignant ameloblastoma than those in benign ameloblastoma ( P

Objective To investigate if vascular endothelial growth factor-D(VEGF-D) and endostatin(ES) promote angiogenesis in colorectal cancer(CRC). Methods One hundred and five patients undergoing CRC surgery were enrolled in this study. CRC and normal tissue microarray were produced and measured for VEGF-D and ES by immunohistochemmical staining.Results VEGF-D and ES expression were stronger in CRC tissues than in control normal mucosa. MVD was higher in cancer tissues than in normal ones. ES down-regulated expression was associated with less invasiveness, positive lymph node metastasis, distant metastasis and in cases of early Dukes′ stage, while the expression of VEGF-D in corresponding tissues was on the opposite to ES. MVD increased along with tumor invasion, lymph involvement, distant metastasis and late Dukes′ stage. Conclusions VEGF-D in accord with ES promotes the angiogenesis and progression of CRC.

Objective:To explore the personnel arrangement in the outpatient pharmacy by calculating working hour to provide ref-erence for the rational staffing in hospital. Methods:The daily work content and working hour of 18 pharmacists in the outpatient phar-macy of a large general hospital from January to March in 2013 were following-up observed and recorded using the working hour meas-urement. The data were input the EXcellsoftware to establish the database, and the workload in various positions was collected and sorted. The obtained relative parameters were used to calculate the needed worker number on the basis of manpower planning model. Results:The research confirmed the mean operation time for 9 work programs in the outpatient pharmacy, and the time for drug dispen-sing and distributing was detailed. The needed number of pharmacists was 13. 29 according to the calculation, plus the officer-in-charge and sanitation workers, the total number was 15. 29(approx. 16). Conclusion:The working hour measurement can scientifically de-termine the time for each job, and the workload should be used as the foundation for configuring personnel qualification and the number in outpatient pharmacy.

Objective To explore the clinical significance of determination of the serum levels of brain (B-type) natriuretic peptide (BNP) and high-sensitivity C-reactive protein (hs-CRP) in patients with acute myocardial infarction (AMI).Methods The clinical data of 60 cases with AMI and 50healthy controls were analyzed retrospectively.The BNP and hs-CRP levels were analyzed in AMI and Killip class,and their relationship with prognosis was investigated.Results The serum levels of BNP,hs-CRP and creatine kinase (CK)-MB) were higher in AMI group than in control group (all P＜0.05),and the levels were lowered after treatment (P＜0.05).The correlation analysis indicated that BNP and hs-CRP had positive correlations with CK-MB (r=0.892 and 0.683,all P＜0.05),and increased along with the severity of Killip classification.The 8 cases (13.3 % ) died,and single factor analysis revealed that BNP (≥56.1 pmol/L),hs-CRP (≥9.0 mg/L),CK-MB (≥75.1 U/L) and Killip classification (grade Ⅲ and Ⅳ) were the poor prognostic factors (P＜0.05).Conclusions The elevation of BNP and hs-CRP level is correlated with the severity of AMI,and could be used to evaluate the AMI patients' diagnosis and prognosis.

Objective To summarize the clinical experience of hepatectomy with hepatic artery resection and reconstruction using gastroduodenal artery during radical resection of hilar cholangiocarcinoma.Methods From Dec.2004 to Dec.2008,nine cases of hilar cholangiocarcinoma with hepatic artery invasion were subjected to radical resection comhined with tumor invaded hepatic artery resection and reconstruction using gastroduodenal artery.The clinical data of these patients were reviewed.Results Nine cases underwent hilar cholangiocarcinoma radical resection with hepatic artery resection,immediate hepatic artery reconstruction using gastroduodenal artery end to end anastomosis while hepatic artery resection exceeding 1 cm.One patient underwent partial resection of the portal vein and repair using autogenous segment of great saphenous vein.Roux-en-Y hepaticojejunostomy was performed in 9 patients with intrahepatic bile duct stents in 8 patients.All patients suffered from postoperative transient SIRS and recovered within 2-3 days after operation.One patient experienced massive bleeding from the upper alimentary tract 3 day after operation and the bleeding was controlled afterwards.The blood flow in the reconstructed hepatic arteries monitored by Doppler was normal two weeks after operation.There was no inhospital mortality.9 patients were followed up for 1-4 years,the median survival time is 23 months (6 months to 32 months).Conclusion Hepatic artery can be reconstructed using gastroduodenal artery during a radical resection of hilar cholangiocarcinoma,and hepatic artery reconstruction decreases the postoperative complications.

Objective: To explore the effects of sensory integration training on the behaviors and cognition in children with attention deficit hyperactivity sorder (ADHD) . Methods: Fifty-two children with ADHD (experimental group) were undergone sensory integration training for 60 times and their behaviors were evaluated with the Corners Parents Symptom Questionnaire (PSQ), and the cognition function was determined with the Combined Raven's Test (CRT) and Wisconsin Card Sorting Test (WCST) before and after the training. Another 52 healthy volunteers were recruited as controls and given the corresponding tests described above for comparison of the results. Results: The scores of conduct disorder, compulsion, hyperactivity and hyperactivity index in children with ADHD were decreased significantly than those of controls after training [(0.60 ± 0. 34) vs. (0.68 ± 0.35), (0.92±0.57) vs. (1.25±0.70), (0.82±0.29) vs. (1.08±0.44); P<0.05] .Inaddition, the improvement was found in IQ, categories control, and conceptive level as compared with that before the training [such as IQ, (105. 37 ±22.76) vs. (97. 37 ±24.15); P<0.001], whereas the scores of response error, response error percentage, first category and persistent errors were decreased after the training [such as response error, (39.40 ± 22.22) vs. (46.60 ±23.44), P<0.001] . For the experimental subjects, the scores in the first response number and the persistent errors percentage right after the training were higher compared with the controls [(12.03 ±5.08) vs. (10.65±0.84), (50.75 ±18.35) vs. (39.95 ± 15. 34); Ps<0.05] .Conclusion: The sensory integration training can improve the behaviors and cognition in children with ADHD, but it produces fewer effects on some of the executive functions despite clinical symptom relief to a certain extent.