The purpose of this study was to research the characteristic of superoxide dismutase (SOD) in erythrocy-tes of sprinters and middle-distance runners. Resting content and activity of SOD in erythrocytes were studi-ed in 8 untrained men, 8 sprinters and 8 middle-distance runners (male). SOD content, as well as SODactivity, in athletes was significantly greater than it in untrained men. It suggested: the exercise trainingwhich glycogen breakdown provided the majority of the non-oxidative energy could elevate the level of SODin erythrocytes. The VO_2 max and AT, and SOD content and activity in erythrocytes from 12 athletes (4sprinters and 8 middle-distance runners) were studied. There was no significant correlation between both VO_2max and AT and SOD level. To also observe the change of SOD in erythrocytes after a bout of exercise.The content and activity of SOD did not change significantly after 12 athletes exercised to VO_2max on acycle ergometer. Similarly, they did not change significantly during 14 hours of recovery from 5000-metrerace. These athletes had trained regularly several years and SOD level in erythrocytes of them had increased,which had been sufficient to remove and cope with increaced O_2 during the exercise. It might be one ofthe causes that SOD in erythrocytes did not change significantly after a bout of exercise.

Objective To compare myocardial protection between cold blood and crystalloid cardioplegia in short aortic cross-clamping(

Objective: To study the effects of static tensile strai n on the secretion of prostaglandin E 2 (PGE 2) of human periodontal ligament fibroblasts (PLFs). Methods: Human PLFs were treated by tensile strain values of 0%,8%,12%,16% and 20% for 24, 48 and 72 h respecti vely with a self-devised loading apparatus in vitro. All samples were rando mly allocated into 15 groups and there were 3 in each group. After treatment cu lture media of the samples were collected and the content of PGE 2 in each medi um sample was determined using RIA ELISA. The data analysis was carried out with SPSS using Dunnett test. Results: In group of 0% the sec retion of PGE 2 by PLFs per day had no relation with loading time; E 2 secre tion increased with the increace of loading time and with the tensile strain va lue in the group of 8%,12% and 16%(P

Objective To observe the role of PGE 1 in heart-lung transplantation. Methods PGE 1 combined with modified LPD solution as the lung flush solution of the donor was applied in heart-lung transplantation. Results PGE 1 combinded with modified LPD solution provided the excellent effect of lung pretection. The patient survived over one year after heart-lung transplantation, and never occurred severe infection, lung disfunction and severe graft rejection. Conclusion The combination use of PGE 1 and modified LPD solution as the lung flush solution in heart-lung transplantation possessed a excellent protective effect for lung.

OBJECTIVE To discuss the effect of regular emptiness of humidification pot on ventilator associated pneumonia(VAP) in the patients with mechanical ventilation.METHODS Totally 240 mechanical ventilation patients were randomed to 2 groups,in an observation group the humidification pots were emptied per 24 hours and in a control group the fluid was added only when its water was lower limit and compared their incidence rate of VAP.RESULTS Ninety-six of 240 patients with mechanical ventilation had developed VAP and 238 pathogen strains were cultured.Altogether,the incidence of VAP in observation group was lower than those in control group significantly,?2=4.4,P

This is a prospective study on early discovery and diagnosis to cerebral palsy(CP).Fivehundred and forty cases with high risk neonates who were born from July 1. 1993 to December 31. 1994 infour districts in Beijing were monitored for one year.Eight cases were diagnosed for CP from 38 suspiciouscases by examining specific primitive reflex. nerve reflex.muscular tension. early symptoms and CT orMRI.etc. The study indicates that it is very important for decreasing CP incidence to strengthen healthmeasures in perinatal period and to avoid premature infant birth.

Heart rate is the most common index to directly monitor the level of physical stress by comparing the subject's heart rate with an appropriate "target heart rate" during exercise. However, heart rate only reveals the cardiac rhythm of the complex cardiovascular changes that take place during exercise. It is essential to get the dynamic response of the heart to exercise with various indices instead of only one single measurement. Based on the rest-workload alternating pattern, this paper screens the sensitive indices of exercise load from electrocardiogram (ECG) rhythm and waveform, including 4 time domain indices and 4 frequency domain indices of heart rate variability (HRV), 3 indices of waveform similarity and 2 indices of high frequency noise. In conclusion, RR interval (heart rate) is a reliable index for the realtime monitoring of exercise intensity, which has strong linear correlation with load intensity. The ECG waveform similarity and HRV indices are useful for the evaluation of exercise load.
Electrocardiography ; Exercise ; Heart Rate ; Humans ; Monitoring, Physiologic ; Workload

Objective To study the proper desiccation and the expiry date for humidifier after disinfection, and then decline the incidence rate of infection in hospital. Methods Divided 84 humidifiers into the A and B groups randomly after disinfection, there were 42 humidifiers in the each group. The natural desiccation was used in the A group, the oxygen desiccation was used in the B group. Monitored the humidifiers of the two groups respectively lasted 7 days, and then compare the datum between them. Results The expiry date of the humidifiers which have dried by natural desiccation was longer than that of humidifiers which have dried by oxygen desiccation. Conclusion We should set down the expiry date for humidifier according to the different desiccation and the different seasons.

BACKGROUND:Spermatogonial stem cells are a kind of adult stem cells, which have self-renewal and differentiation potential, and can be differentiated into specific cells in vitro, suggesting that the spermatogonial stem cells may be possibly differentiated into osteoblasts. But the related research has not been reported. OBJECTIVE:To observe the biological characterization and osteogenic process of mouse spermatogonial stem cells cultured in vitro. METHODS:Spermatogonial stem cells were obtained from the testicle of mice aged 15-20 days, and were cultured on the feeder layer from bone marrow stroma cells in vitro. When cultured for 3 days, the cells were cultured in the conditioned medium (experimental group) and basic medium (control group). The cells proliferation capability and osteogenic property were examined by phase-contrast microscope, alkaline phosphatase activity and type I col agen immunofluorescence staining. RESULTS AND CONCLUSION:Spermatogonial stem cells proliferated faster in the experiment group than in the control group. cells grew rapidly in colony-like shape in the conditioned medium at 3-6 days, the three-dimensional feeling enhanced, cellmass and clusters continued to increase in size, the extracellular matrix was increased in number and the cytoplasmic bridge was not obvious. After culture for 15 days, cells in the two groups were positive for alkaline phosphatase staining that the cytoplasmic membrane was dyed black. Under the fluorescent microscope, green fluorescence was visible in the experimental group, suggesting the cells in the experimental group was positive for type I col agen, but negative in the control group, which is similar with the biological characteristics of osteoblasts. These findings indicate that spermatogonial stem cells possess the osteogenic capability under induction conditions, which are expected to provide seed cells for bone tissue engineering.

ObjectiveTo explore an individualized abdominal scan protocol with a 64-slice CT scanner.MethodsFrom Sep.2010 to Nov.2010,one hundred consecutive patients,who underwent twice non-contrast-enhanced abdominal CT scans within 3 months,were enrolled in this study.For each patient,the tube current of 274 eff.mAs and 207 eff.mAs were applied respectively in the first and second abdominal scan.The imaging qualities of the two scans were evaluated retrospectively by 3 reviewers.All the individual variants,including height,weight,body mass index (BMI),the maximum transverse diameter,the anteroposterior diameter and the average maximum diameter of abdomen were recorded.A five-point scale was used for grading the image noise of eight organs,including abdominal aorta,portal vein,liver,spleen,gallbladder,pancreas,renal cortex and renal medulla. Diagnostic acceptability of CT images at three anatomic levels,including porta bepatis,pancreas and the upper pole of renal,was also evaluated by using a five-point scale.The noise value of abdominal aorta was defined as the standard deviation (SD) of CT values of aorta at the level of porta hepatis.Scatter diagram and Pearson correlation analysis were used for evaluating the linear relationship between the individual variants and the noise value of abdominal aorta,and multivariate linear regression analysis was used for evaluating the relevance between the individual variants and the noise value of aorta.ResultsIn this patients group,the average height was ( 164.6 ± 7.5 ) cm,the average weight was (64.3 ± 11.0) kg,the BMI was (23.7 ±3.3) kg/m2,the maximum transverse diameter of abdomen was(29.8 ± 2.3 )cm,the anteroposterior diameter of abdomen was (23.1 ± 2.9) cm,and the average maximum diameter of abdomen was ( 26.5 ± 2.5 ) cm.Pearson correlation analysis showed significant positive linear correlation between the noise value of abdominal aorta( 1 1.7 ± 3.0)and patients' weight ( r =0.744,P ＜ 0.01 ),BMI ( r =0.689,P ＜ 0.01 ),the maximum transverse diameter ( r =0.813,P ＜ 0.01 ),the anteroposterior diameter ( r =0.781,P ＜ 0.01 ),the average maximum diameter of the abdomen ( r =0.789,P ＜ 0.01 ) ; however,there was no positive linear correlation between the noise value of abdominal aorta and patients' height ( r =0.292,P ＜ 0.01 ). The maximum transverse diameter of abdomen is greatly related to the noise value of abdominal aorta (Beta =0.487,P ＜0.01 ).For the patient with the maximum transverse diameter of abdomen ranging from 27 to 32 cm,diagnostic acceptability of CT images at the anatomic level of porta hepatis showed statistical significance compared with the patient with the maximal transverse diameter of the abdomen greater than 32 cm or less than 27 cm (P ＜ 0.05 ).Conclusion The tube current of 207 eft.mAs is reasonable for abdominal CT scan for patients with the maximal transverse diameter of the abdomen ranging from 27 to 32 cm.