Objective To observe the therapeutic efficacy of bronchial artery embolization(BAE) and pulmonary artery embolism(PAE) for massive hemoptysis.Methods 48 cases with massive hemoptysis were treated with embolization,of them,43 underwent selective bronchial arteriography and BAE with microballoon of Baiji,three cases underwent selective bronchial arteriography and treated with PAE at the same time,two cases underwent PAE.Results The abnormal features of arteriography were displayed in all hemoptysis stopped after operation 48 h later.Three cases recurred during followed up six months later,but the hemoptysis decreased.Conclusion Pulmonary arteriography and PAE therapy should be preformed when BAE fails for massive hemoptysis.

ObjectiveToevaluatetheclinicalvalueoflaparoscopicrenalcystdecorticationandopencyst decortication surgery in the treatment of polycystic kidney disease.Methods Various clinical parameters were retro-spectively analyzed in 108 patients with polycystic kidney,include laparoscopic 60cases and open surgery 48cases.The observation indexes include operative time,operative bleeding,restoration time of bowel functions,postoperative drain-age volume,hospital time,postoperative complications,kidney function,blood pressure,and the clinical effects were estimated.Results In the laparoscopic group,the average operation time was (57.7 ±13.2) min,the average blood was (35.9 ±17.3)mL,the average time of exsufflation was (1.6 ±0.7)d,the average postoperative drainage volume was (23.2 ±4.3)mL,the average hospital time was (5.1 ±0.8)d.As compared with the open surgery group,the op-eration time,hospital stay,operative bleeding,postoperative drainage and hospital time of the laparoscopic group short-ened significantly(all P<0.05).The complication rate of the laparoscopic group and open surgery group were 5.0%and 16.7%separately.The complication incidence was significantly lower in the laparoscopic group than that in the open surgery group(P<0.05).At 3 months postoperative,the levels of serum creatinine(Cr),urea nitrogen(UN)and systolic blood pressure were obviously decreased compared with preoperative.There were no significant difference be-tween the two groups(P>0.05).The follow-up data showed that the cure rate and effective rate of the open surgery group were 81.2%and 18.8%,and the cure rate and effective rate of the laparoscopic group were 85.0% and15. 0%respectively.There were no significant difference of the clinical effects between the two groups (P >0.05). Conclusion The laparoscopic was superior to the open surgery in the operation time,hospital time,operative bleed-ing,postoperative drainage and hospital time.Laparoscopic renal cyst decortication in polycystic kidney is open surgery safer,little injury,quick recovery.Laparoscopic renal cyst decortication is safe and effective therapy,which can be used as primary surgical treatment for patients with polycystic kidney.

Objective To observe the protein degredation of empty puparium cases ofC. megacephala after weathering and to explore its practical significance on postmortem interval estimation in forensic science. MethodsThe standardized feeding was used inC. megacephala, and the empty puparium cases were collected and put into the forest. They were taken back 5 days, and 10 days later, respectively. The spectra were collected and preprocessed, and then the absorption peaks of amides were read, and curve fitting was performed in the average spectra of each group. The statistical analysis was performed by SPSS 19.0.Results Compared to control group, ifve days post weathering, the position of amideⅠabsorption peak showed blue shift, but the amideⅡabsorption peak showed no shift, moreover, none of the amide absorption peaks showed changes inpeak intensity. For the secondary structures, α-helix decreased and β-sheet increased slightly, however, β-turn did not change; Ten days post weathering, the positions of both amideⅠandⅡabsorption peaks showed blue shift, and an obviousshoulder peak appeared in amide I absorption peak. Meanwhile, both of amideⅠandⅡabsorption peaks showed decreased in peak intensity. Moreover, for the secondary structures, α-helix and β-sheet showed the same tendency as in the 5th day group, except for the β-turn increased dramaticlly.Conclusion The spectra of the empty puparium cases of C. megacephala showed that the absorption peaks of amideⅠandⅡpresented certain characteristic features within ten days post weathering, and estimate relative long-time postmortem interval.

Objective To explore the value of lipidic degradation in puparium cases of C.megacephala during weathering in postmortem interval estimation. Methods The puparium cases of reared C.megacephala were collected, and laid outdoor. They were taken back 5 days and 10 days later, and then be stored at -20℃. The samples of control group were cleaned and cryopreserved immediately. The spectra were collected and preprocessed. The peak position and peak height were read and performed statistical analysis by SPSS 19.0 software. Results Compared to control group, the positions of Vas CH3 band showed no shift, and the Vas CH2 band showed blue shift, meanwhile, the Vs CH2 band disappeared in both experimental groups, moreover, the Vs CH3 band showed red shift only in 10d group;except of the Vs CH3 band in 5d group, the intensities of both the other two lipidic bands reduced in both experimental groups. Compared with 5day group, the Vs CH3 band showed red shift in 10d group, meanwhile, the differences of all the lipidic bands had statistical signiifcance. Conclusion Detection of lipidic degradation in puparium cases of C. megacephala during weathering by micro-FTIR provides a novel way to estimate postmortem interval and performs its potential for forensic applications.

Objective To detect the molecular characteristics of the serum samples of rats suffered from sudden unexpected death of acute myocardial ischemia (experimental group) and death caused by air embolism (control group) applied by ATR-FTIR, and to discuss the forensic signiifcance of identiifcation of causes of death by sudden unexpected death of acute myocardial ischemia by two-dimension infrared spectroscopy. Methods Adult male SD rats were randomly divided into SCD group and DCAE group. The serum samples were analyzed by ATR-FTIR. The similarity coefifcient of spectra between two groups was analyzed by OMNIC software. The spectra with higher signal-noise ratio were obtained and used to build two-dimension spectra model by Matlab 2010a software. Results The peak shapes, positions and intensities of the spectra were extremely similar between two groups, and the similarity coefifcient reached 99.78%. Meanwhile, the similarity coefifcient of second derivative spectra decreased to 98.62% between two groups. Moreover, on the two-dimension spectra model, the component differences were present at about 1 625cm-1, 1 550cm-1, 1 080cm-1 and 860cm-1, and the number of auto-peaks was different, which suggested that the evident differences were related to the molecular contents of protein, glycogen, nuclear acid, and so on. Conclusion It is hard to differentiate the molecular differences of serum samples of rats between SCD and DACE based on one-dimension spectra. However, two-dimension infrared spectra could intuitively and effectively relfect the molecular differences, and it would apply to determining the cause of death in forensic science.

Objective To evaluate the effect of slow-release microcapsules of HCF( hepatocyte growth factor) on angiogenesis in infracted myocardium.Method Myocardial infarction was induced in 30 New Zealand rabbits by ligating the middle of left descending coronary artery. Group Ⅰ ( n = 10) was served as a control group, group Ⅱ ( n =10) as a blank microcapsule group, group Ⅲ ( n = 10) as experimental group with each microcapsule contains 1 μgHGF as HCF group. In group Ⅱ andⅢ, 5 blank microcapsules or FGF slow-release microcapsules were implanted into myocardium under epicedium between the left descending coronary artery and left circumflex branch. The heart function of each rabbit was evaluated with echocar-diography and cheterization, angiogenesis was evaluated by immunohistochemical technique 6 weeks later.Result As compared with group Ⅰ and Ⅱ , rabbits treated with HGF had higher microvessel counts ( P < 0. 01), and LVFS and EF were significantly increased [ (101. 28±19. 50,105. 28 ±18. 28,161. 28 ±15. 85, P <0.01 ]. Conclusion Subepicardial implantation of HGF slow release microcapsule in the infracted rabbit model can enhance effective angiogenesis and improve left ventricular function.

This study was aimed to assess the effects of remain of residual tooth on the retention of postpost-core was made,casted and cemented. The post-core was tested at the speed of 10 mm/min on the Instron4302, and the retention force was recorded. Other samples were made and subjected to the test for cohesion of polymethylmethacrylate and metal. The result showed that retention significantly increased with the adding of the residual tooth. When the length of post was longer than 8 mm and 1 mm was added to tooth remain, the difference in retention between the two groups was statistically significant. It was noted that there existed relationships of retention to length of post and tooth remain, of cohesion to length of post and tooth remain, and of frictiion resistence to length of post and tooth remain; moreover, the relevant linear regression equations were obtained. So during the tooth preparation of post-core system, the tooth tissue should be reserved as much as possible.
Crowns ; Dental Prosthesis Retention ; Dental Stress Analysis ; Humans ; Post and Core Technique ; Tooth Root

To study the effects of different post design on retention. 27 simulated tooth roots made of PMMA were divided into three groups, then manufactured the cement-expanded composite screw post-core system (CECSP), cement screw post-core system (CSP) and cast metal post-core system (CMP). The root and post-core system was tensiled by the speed of 10 mm/min on the Instron 4302 and recorded the retention force. It appeared significantly different between retention force of CECSP and CSP and CAM (P < 0.05). As a result, the retention mechanical properties of CECSP was much better than those of cement screw post-core system and cast metal post-core system. Therefore, it is possible to find a new way for restoration of endodontically treated teeth.
Acrylic Resins ; Cementation ; Composite Resins ; Crowns ; Dental Materials ; chemistry ; Dental Prosthesis Retention ; Dental Stress Analysis ; Humans ; Polyurethanes ; Post and Core Technique ; Tensile Strength

OBJECTIVE To investigate the effect and mechanism of Panax notoginseng saponins （PNS） on wound healing after anal fistula surgery in rats by regulating the hypoxia-inducible factor-1α （HIF-1α）/vascular endothelial growth factor （VEGF）/ vascular endothelial growth factor receptor-2 （VEGFR2） signaling pathway. METHODS SD rats were selected to establish a postoperative rat model of anal fistula by infecting wound with Escherichia coli. The model rats were randomly grouped into model group， PNS low-dose and high-dose groups （15， 30 mg/cm2）， high-dose of PNS+2-methoxyestradiol （2ME2） group （PNS 30 mg/cm2+HIF-1α inhibitor 2ME2 4 mg/kg）， with 10 rats in each group. Another 10 normal rats were selected for back hair removal treatment as the control group. Each drug group was injected with the corresponding drug solution intramuscularly or （and） intraperitoneally， once a day， for 3 weeks. After the last administration， the wound healing rate （excluding the control group）， microvascular density （MVD）， the expression of collagen Ⅰ and fibronectin （FN） in the wound tissue were detected in each group； the levels of angiogenic factors [VEGF， E-mail：842710813@qq.com angiopoietin-Ⅰ （Ang-Ⅰ）， Ang-Ⅱ] in serum， the levels of inflammatory factors [interleukin-6 （IL-6） and IL-2] in serum binggui7183@163.com and wound tissue as well as the expressions of the related proteins of HIF-1α/VEGF/VEGFR2 signaling pathway in the wound tissue of rats were also detected in each group. RESULTS The MVD， the expression of collagen Ⅰ and FN in the wound tissue， and the levels of IL-6 and IL-2 in serum and wound tissue of rats increased significantly in the model group， compared to the control group （P＜0.05）， while the serum levels of VEGF， Ang- Ⅰ and Ang-Ⅱ decreased significantly （P＜0.05）. The wound healing rate， the MVD in wound tissue， the serum levels of VEGF， Ang-Ⅰ and Ang-Ⅱ， the expressions of collagen Ⅰ and FN in the wound tissue， and protein expressions of HIF-1α， VEGF and VEGFR2 in the PNS low-dose and high-dose groups increased significantly， compared to the model group （P＜0.05）， while the levels of IL-6 and IL-2 in serum and wound tissue decreased significantly （P＜0.05）； the high-dose PNS had a stronger effect （P＜ 0.05）. 2ME2 could weaken the effect of PNS on above indicators of rats after anal fistula surgery （P＜0.05）. CONCLUSIONS PNS can promote the production of angiogenic factors and inhibit the production of pro-inflammatory factors， thereby promoting wound healing in rats after anal fistula surgery. The above effects are related to the activation of HIF-1α/VEGF/VEGFR2 signaling pathway.

AIM: To evaluate the changes in corneal epithelial thickness(CET)and corneal optical density(CD)after smart pulse technology(SPT)-assisted transepithelial photorefractive keratectomy(TPRK)and analyze their correlation.METHODS: The prospective study included 60 patients(120 eyes)with myopia and myopic astigmatism who underwent SPT-TPRK in the ophthalmology department at the First Affiliated Hospital of Xinxiang Medical University between February and August 2023. Changes in CET and CD were evaluated preoperatively and at 1 wk, 1 and 3 mo postoperatively.RESULTS: A total of 14 cases(28 eyes)were lost to follow-up, and 3 patients(6 eyes)with postoperative haze were excluded from this study, resulting in a final inclusion of 43 patients(86 eyes). At 1 wk after SPT-TPRK, CET had statistically significantly thickened compared to preoperative levels(P<0.05), particularly in the CET at 0-2 mm central corneal area(P<0.05). At 1 mo after SPT-TPRK, the CET at 0-2 mm area had statistically significantly decreased(P<0.05). At 3 mo after SPT-TPRK, the CET at 0-2 mm had essentially reached preoperative levels. Postoperative CD values increased, with a positive correlation between CET in the 0-2 mm area and CD in the whole 0-2 mm area(r=0.256, P<0.05), and a positive correlation between CET in the 2-5 mm area and CD in the anterior 2-6 mm area(r=0.319, P<0.05).CONCLUSION: Corneal epithelial remodeling takes 3 mo in areas within 2 mm of the central cornea; areas with thinner CET have faster postoperative corneal epithelial remodeling and greater thickening in the early postoperative period; CD increases in the early postoperative period compared to the preoperative value, and in some areas, there is a positive correlation between CET and CD value.