The effects of decreasing .blood viscosity through hemodilution on coronary collateral flow and myocardial injury in the.early phase of myocardial ischomia were studied in 21 open-chest anaesthetized dogs. It was shown that after 30 rain of coromary occlusion the low-shear viscosity of the whole blood (?bl) began to increase gradually, while the collateral flow (CF) decreased concomitantly. At the 75-min postocclusion, ?bl increased by 18.1?3.9%, CF decreased by 24.1 ?4.6%, ST-segment of epicardial electrogram elevated significantly and the ultrastructural changes were characterized by severe mitochondrial damage, autolysis of myofilaments and blood cell obstruction in the micro vessel etc. In the hemodilution group, the chamges i1~ ~qbl and CF of the first 30-min postocclusion were quite the same as that of the control group. Following mild isovolemic hemodilution performed at the 30-min postocclusion, all of the ischemic changes improved significantly. At the 75-min postocclusion, contrary to the control group, ?bl decreased by 25.5?3.9% and CF increased by 54,7?10.8% as compared to the control value, the previously elevated ST segment decreased markedly and the ultrastructural injury alleviated to a great degree as well. The experimental results suggest that the deleterious hemorheologic changes in the early phase of myocardial ischemia is a major factor of aggravating ischemic myocardial injury and the decrese in blood viscosity through hemodilution may have protective effect on ischemic myocardium by increasing the collateral flow.

Following acute occlusion of the left anterior desending coronary artery, the significant and constant hemorrheologic changes were observed in blood draining from ischemic area. At the 40-min of occlusion, the tow shear viscosity (r=1.15 s~(-1)) of whole blood increased from the preocclusion value of 39?7.9m Pa.s to 58?8.4 mPa.s (P

The changes in flow properties of blood draining-from ischemic area and from systemic circulation causad by acute coronary occlusion were studied in 21 anesthe- tized open-chest dogs and the role of cardiac-cardiac sympathetic reflex in these changes was analysed. The main results obtained were as in the following: 1. The obvious hemorhoblogic changes were detected at the early phase of myocardial ischemia and the more significant change in high shear viscosity of whole blood was observed in coronary venous blood draining from ischemic area; 2. Topical application of lidocaine to the ischemic area of the heart virtually abolished the hemorheologie changes in systemic venous blood caused by coronary occlnsion, but had no significant effect on the change in high shear viscosity of blood draining from the ischemic area; 3. Bilateral section of stellate ganglia had no effect on the changes in hemorheologe following coronary occlusion. The results mentioned above suggested that increased activity of cardiac sympathetic nerves which originated from ischemic area had an important role in causing hemorheologic changes following coronary occlusion.

The neuromuscular and cardiovascular effects and endotracheal intubating conditions of bolus intravenous rocuronium 0.6mg/kg or 0.75mg/kg in 20 patients under balanced anesthesia,were studied, Intubating conditions were evaluated as excellent or good in all patients,except for one with poor intubating conditions following 0.6mg/kg. Onset times of both groups were 73.1 s and 67.1 s;neuromuscular blockade durations 24.9min and 32.0min; 25% recovery times 37.3min and 45.4min; 75% recovery times 46.4min and 58.7min;recovery index 9.1min and 13.3min,respectively. The cardiovascular effects after rocuronium in both groups were minimal.

Objective To explore the curative effects of microsurgical clearance for supratentorial hypertensive intracerebral hemorrhage. Methods A retrospective analysis of clinical data of 30 cases of supratentorial hypertensive intracerebral hemorrhage in our hospital from January 2010 to January 2013 was carried out. According to the head CT images, the position of incision was determined. Under orotracheal intubation general anesthesia, a bone flap craniotomy was performed. A cortex fistula 2-3 cm in length was made. Then by using an automatic retraction device, the hematoma was revealed from the shallower to the deeper, and microscopic clearance of hematoma was completed. Results All the 30 cases of operation were successful. Re-examination of head CT scans on the first postoperative day showed hematoma was completely removed in 14 cases, was 90%cleared in 11 cases, and 80%in 5 cases. Four patients died within 4 weeks after operation (13.3%):2 patients died of cerebral hernia caused by brainstem function failure, 1 patient died of lung infection, and 1 patient died of multiple organ failure. The postoperative survival rate was 86.7%.The ADL grade assessment 3 months after operation showed gradeⅠin 7 cases, grade Ⅱin 9 cases, grade Ⅲin 6 cases, grade Ⅳ in 3 cases, and grade Ⅴin 1 case. Conclusion Microscopic hematoma clearance and open haemostatic intervention has advantages of little brain tissue damage, good haemostatic results, and satisfactory efficacy.

OBJECTIVE To investigate the causes of stomal recurrence after laryngectomy and the possibility of surgical management.METHODS The clinical data of 13 cases of stomal recurrence were analyzed retrospectively.RESULTS In 13 cases of stomal recurrence after laryngectomy,the survival period of 9 cases treated surgically was longer than that of 4 cases untreated or palliative treatment.CONCLUSION The overall prognosis of stomal recurrence after laryngectomy is poor.A proper surgical treatment in some cases will prolong the patient's lifetime obviously and improve his quality of life.

OBJECTIVE:To establish a method for determination of the contents of chlorogenic acid and baicalin in Shiwuwei Xiaoyanzhike oral liquid .METHODS:HPLC was used,the mobile phase was acetonitrile - acetic acid,the flow rate was 0.8ml/ min and the detecting wavelengths were 326nm and 274nm.RESULTS: The recoveries of chlorogenic acid and baicalin were 96.8% - 104.5% , 94.9% -97.7% and RSD were 1.09% -3.12% , 1.19% -1.76% (n = 3), respectively .CONCLUSION: The method is simple and feasible with a good reproducibility, the RESULTS: can provide a basis for establishing the quality standard of this oral liquid.

Congresses as Topic ; Head and Neck Neoplasms ; Humans

OBJECTIVETo study the pharmacodynamics of vecuronium,atracurium, mivacurium and rocuronium in patients with end-stage renal failure.

METHODSForty-six patients with end-stage renal failure scheduled for renal transplantation and 53 patients with normal renal function were given either vecuronium, atracurium, mivacurium or rocuronium. The neuromuscular effects were monitored by the evoked response of the adductor pollicis to train-of-four stimulation of the ulnar nerve.

RESULTSOnset of vecuronium, atracurium and mivacurium occurred faster or tended to be faster in patients with end-stage renal failure, but there was no significant difference in onset by rocuronium between the control patients and renal failure patients. Furthermore, the no-response period, duration of action and recovery of atracurium did not differ between the two groups. There was no significant difference in duration of action or recovery of mivacurium between the two groups, whereas its no-response period was significantly prolonged in the patients with end-stage renal failure. There was no difference in no-response period or duration of action after the initial dose of vecuronium or rocuronium between the two groups. However, no-response period and duration of effect by vecuronium and rocuronium were prolonged with increasing incremental doses in patients with end-stage renal failure.

CONCLUSIONSAll four muscle relaxants could be safely used in patients with end-stage renal failure. Onset of the relaxants were, in some cases, accelerated and no-response period of mivacurium was prolonged in patients with end-stage renal failure undergoing dialysis therapy. End-stage renal failure prolonged the no-response period and duration of action of vecuronium and rocuronium after repeated incremental doses, but did not alter those attributed to atracurium.

Adult ; Androstanols ; pharmacology ; Anesthesia, General ; Atracurium ; pharmacology ; Female ; Humans ; Isoquinolines ; pharmacology ; Kidney Transplantation ; Male ; Middle Aged ; Neuromuscular Blocking Agents ; pharmacology ; Succinylcholine ; pharmacology ; Time Factors ; Vecuronium Bromide ; pharmacology

To study the effect of genistein on the proliferation of cardiac fibroblasts(CF), CFs were cultured from neonatal rat hearts, DNA synthesis of the cells was determined by incorporation of ［3H］TdR into DNA, the cell cycle was measured by flow cytometric analysis. Genistein(0.5－50 μmol*L-1) attenuated 2.5% fetal calf serum-induced proliferation of CF in concentration-dependent manner. Genistein(50 μmol*L-1) arrested CF cell progression at G2/M phase. The results suggest that genistein be a potential substance for treatment of cardiac fibrosis.