Objective To research the efficacy and safety of percutaneous puncture kyphoplasty in the treatment of elderly osteoporotic vertebral fracture.Methods PkP were performed in 23 patients(34 vertebrae),visual analogue scale(VAS)and mobility were evaluated before and three days after operation.Relief of back pain,recovery of mobility and complications(cement leakage,adjacent vertebral body fractures,etc)were observed in the follow up.Results All patients were successfully performed with immediate relief of their back pain.The mean scores of VAS and mobility 3 days after operation were respectively decreased from(7.5 ±0.7),(2.4 ±0.6)preoperative to(2.5 ±0.6),(1.2 ±0.4)at 3 day after operation with a significant difference(t= 23.8,13.4,P ＜0.01).The patients were followed up for average 15.6 months with satisfactory therapeutic effect,one patient died of heart disease two months after discharge,around vertebral fracture occurred in 4 patients,they were no cement leakage in all the patients.Conclusion PKP is one of the new minimally invasive technologies,which can rapidly relieve pain.PKP is an ideal method in treating elderly osteoporotic spinal compression fractures.

Purpose To detect the expression of peroxiso-mal membrane protein 4(PXMP4)in breast cancer tissues and to explore the effect of PXMP4 on the proliferation,invasion,and epithelial-mesenchymal transition(EMT)of breast cancer cells.Methods Bioinformatics and immunohistochemistry(IHC)were used to detect the expression of PXMP4 in breast cancer tissues.In breast cancer cells,Western blot was used to detect the expression of Cyclin D1,E-cadherin,vimentin and N-cadherin after knockdown and overexpression of PMXP4.The proliferation ability of breast cancer cells was analyzed by CCK-8 and plate cloning assay.Scratch healing and Transwell assay an-alyzed the migration and invasion ability of breast cancer cells.Lentivirus was used to construct a PXMP4-silenced MCF-7 cell line,and the PXMP4-silenced MCF-7 cells were injected into the subcutaneous or tail vein of mice to observe lung metastasis and the number of subcutaneous tumors.Results Bioinformat-ics and IHC showed that the expression of PXMP4 in breast cancer tissues was significantly increased(P＜0.05),and the prognosis of breast cancer patients with high expression of PXMP4 was poor(P＜0.05).The clinicopathological analysis showed that the expression of PXMP4 was correlated with tumor grade and lymph node metastasis(P＜0.05).In vitro knock-down of PMXP4 inhibited the proliferation,invasion and EMT process of breast cancer cells(P＜0.05).Conversely,overex-pression of PXMP4 promoted the proliferation,invasion and EMT process of breast cancer cells(P＜0.05).In vivo,the number of lung metastases,the size of subcutaneous tumor,and the expression of Ki67 in tumor tissue were significantly de-creased after silenced PXMP4(P＜0.05).Conclusion PXMP4 is related to tumor grading and lymph node metastasis.PXMP4 promotes proliferation,invasion and EMT process of breast cancer cells.