Objective To evaluate the myocardial protective effect of hyperpolarized arrest induced with ATP-sensitive potassium channel opener ,pinacidil in vitroMethods Twenty-four rabbit hearts were randomly divided into three groups : hypothermic hyperpolarized group (LH group), normothermic hyperpolarized group ( WH group), and hyperkalemic control group (group C) The isolated rabbit hearts with a Langendorff apparatus were perfused oxygenated Krebs-Henseleit's solution (K-H) for 10 min and followed by cardioplegia The cardioplegia consisted of StThomas solution with either traditional high potassium (16mmol/L KCl, 4 ℃, group C), or pinacidil 50?mol/L (4 ℃ in LH group or 37 ℃ in WH group ) with 5mmol/L KCl The hearts were subjected to 40 min perfusional occlusion and followed by 20 min reperfusionResults Hearts were arrested quickly in LH group, and a little slowly in WH group, but rebeated quickly , which were different significantly from those in group C ; Recovery of LVP and left ventricle contracility in LH group and WH group were remarkedly faster than those in group C (P

Urea clearance index (KT/Vurea) and creatinine clearance weekly (Ccr) are main indexes to evaluate dialysis adequacy. In order to discuss whether they are suitable to evaluate peritoneal dialysis adequacy, we applied trans-peritoneum transport kinetic model and explored the transport characteristics of fluid and various solutes. We found that: (1) There was no specific relationship among the removal of solutes with different molecular weights; (2) There was significant difference between urea removal and fluid and sodium removal. Our results suggest that urea and creatinine removal do not represent other solutes and fluid removal. KT/Vurea and Ccr may thus not suit to be used alone to evaluate peritoneal dialysis adequacy.
Biomarkers ; metabolism ; Blood Urea Nitrogen ; Creatinine ; metabolism ; Humans ; Peritoneal Dialysis, Continuous Ambulatory ; Urea ; metabolism

Clinical teaching is the critical stage for medical students turning to qualified doctor.In order to overcome the objective problems of insufficient clinical case resources,using the electronic medical record system to collect cases,the real case library of kidney disease was initially constructed,which was presented in the form of network resources.This case database was applied to assist teaching in the probation of eight year medical students at the undergraduate stage,and the application of the case database was evaluated in the form of questionnaire.It is found that case database is helpful to students' clinical learning,and has the necessity of further improvement and good prospects for popularization.It provides a new idea for clinical teaching.