Objective To observe the effect on succinate dehydrogenase (SDH) of mitochondria in myocardium and liver in sepsis rats treated with edaravone. Methods 30 Sprague-Dawley rats were divided into 3 groups: sham operated group ( group A ), controlled operated group ( group B ), treated group with edaravone (group C). The model of sepsis rats was made by the way of caecum ligated and punctured and 20mg/kg lactate levofloxacin was subcutaneously injected (sci) 15min before and 3h after operation in three group. 5mg/kg edaravone were sci 15min before and 3h after operation in group C. Liver and myocardium were taken from all of them 18h after operation. The activities of SDH in myocardial and hepatic mitochondria were detected, pathological change of mitochondria in liver and myocardium were observed. Results The activities of SDH in myocardial and hepatic mitochondria in group B [ (0. 21 ± 0. 07 ) U/mgprot, (0. 23± 0. 08 ) U/mgprot ] were significantly decreased compared with group A [ ( 0. 33 ± 0. 10 ) U/mgprot, ( 0. 38±0. 12)U/mgprot]. The activities of those in group C[ (0.31 ±0. 08) U/mgprot, (0. 36 ±0. 11)U/mgprot] were significantly increased than group B. Myocardial and hepatic mitochondria swelling and endocytoplasmic reticulum expanding were found in group B by electron microscope, while it showed normal in group C. Conclusion Hepatic and myocardial mitochondrial structure were destroyed and activities of SDH were decreased in sepsis rats. They could be effectively protected by edaravone.

Objective To investigate the differential expression of P57kip2 and CDK5 in neural tube defects t(NTD) from the normal ,and provide the clue for the research of the molecular mechanism of the normal neurula formation .Methods A cDNA mi-croarray containing 1 100 known genes was used to compare differences in P57kip2 and CDK5 gene expression between the normal control group and the retinoic acid(RA)-induced NTD group on embryonic(E) day 9 .5 and 10 .5 .Two differentially expressed genes were randomly selected from the two groups for Northern blotting to verify the results of the cDNA microarray .Results Compared the differences of between P57kip2 and CDK5 in normal and E9 .5 d ,E10 .5 d ,E9 .5 d-NTD ,E10 .5 d-NTD ,P57kip2 and CDK5 expression was significantly up-regulated in the before and after the formation of the normal neurulation ,but them showed a downward trend in retinoic acid (RA)-induced NTD(including two phase E9 .5 d and E10 .5 d) .Conclusion P57kip2 and CDK5 in-volved in the physiological process of NTD ,and provide the useful clue for the research of the molecular mechanism of the normal neurula formation .

OBJECTIVETo investigate the quality and quantity changes of Flos Lonicerace during flowering stages in Hanzhong GAP base in order for properly harvesting and quality control.

METHODThe yield index of Flos lonicerace in four flowering stages was determined, and the contents of chlorogenic acid and the total flavonoid in the samples were determined by HPLC and the colorimetry, respectively. The volatile oils were extracted by the SFE-CO2 and the constituents were analyzed by GC-MS.

RESULTThe yield in the first two flowering times was attributed to about more than 80% of total yield, and the number of flower buds for each plant in the first time was almost the same as the sum of the other three times. The drying rate of the three-site green flowers kept the highest during the first flowering time, and the drying rate of the two-site white flowers kept the highest during the second flowering time. The contents of chlorogenic acid and the total flavonoid in the first flowering time were higher than those in the second, and among them, the two-site white flowers kept the highest, about 3.5% and 13.2%, respectively. For the sample flowers in in the second flowering time, the three-site green flowers kept the highest level of chlorogenic acid and the total flavonoid, about 2.7% and 11.6%, respectively. From the volatile oil samples in the five periods of Flos lonicerace in the first flowering time, 19 components were identified by GC-MS. They were composed by three types of compounds, n-diolefines, fatty acids and steroids. Nonacosane and hentriacontane had the relatively higher amount with more than 40% and 20%, respectively. The relative contents of vitamine E were higher too, about 8.15% - 10.43%. And, gamma-5-sitostene-3-ol, stigmasta-5,2-diene-3-ol and campesterol were also detected. Among these steroids, the relative contents of the first two were 4.90% - 6.9%, 1.06%- 4.10%, respectively.

CONCLUSIONThe flowering samples in the first two times were attributed to the most to the total yield. The samples of the two-site white flower and the whole-white flower had the higher comprehensive quality. The components of vitamine E and the steroids in the volatile oil need to be paid more attention.

Biomass ; Flowers ; chemistry ; growth & development ; Lonicera ; chemistry ; growth & development ; Oils, Volatile ; analysis ; Seasons

The aim of this study was to establish a high performance liquid chromatography-mass spectrometry method for the determination of 5-(4′-(bromomethyl)-［1, 1′-biphenyl］-2-yl)- 1H-tetrazole(BBT1)and 5-(4′-(dibromomethyl)-［1, 1′-biphenyl］-2-yl)-1H-tetrazole(BBT2), which are two genotoxic impurities in olmesartan medoxomil. Chromatographic separation was based on an Agilent Zorbax Eclipse Plus C18(250 mm × 4. 6 mm, 5 μm)column using water(containing 0. 1% formic acid)- acetonitrile as mobile phase in gradient elution mode. Mass spectrometry was operated in positive ion mode. Selective ion monitors were set at m/z 315 for BBT1 and at m/z 395 for BBT2. Good linear correlations were observed in the range of 0. 009 4- 0. 561 0 μg/mL(r=0. 998)with the quantification limit at 9. 35 ng/mL and the detection limit at 3. 12 ng/mL for BBT1, and in the range of 0. 018 2- 0. 547 5 μg/mL(r=0. 999)with the quantification limit at 18. 25 ng/mL and the detection limit at 6. 08 ng/mL for BBT2. Furthermore, the average recoveries of the three spiked concentration level were 96. 5%(n=9, RSD=4. 8%)and 98. 0%(n=9, RSD=5. 1%)for BBT1 and BBT2, respectively. The proposed method is simple, specific and accurate, and quite suitable for the determination of BBT1 and BBT2 in olmesartan medoxomil.