Objective:To observe and summarize the therapeutic effects of intratumoral injection with Triamcinolone Acetonide Acetate and Pingyangmycin on infant maxillofacial hemangioma.Methods:Local injection with Triamcinolone Acetonide Acetate(TAA)and Pingyangmycin(PYM)was executed to treat infant maxillofacial hemangioma.If the lesion did not disappear,the treatment would be performed again every four to six weeks at intervals,and the total dose of TAA and PYM could not be more than 100mg and 40mg respectively.The injection treatment was repeated 4 times for one course and the therapeutic effects of maxillofacial hemangioma were detected.Results: 84 cases with maxillofacial hemangioma,treated by local injection with TAA and PYM,had been followed up for one to three years.The cure rate was 90.4%,the notable rate was 7.1%,and the ineffective rate was 2.4%.Conclusions:The intratumoral injection with Triamcinolone Acetonide Acetate and Pingyangmycin is the reliable therapy method with less side effects.It is valuable for treatment of infant maxillofacial hemangioma.

Objective:To present the experience in diagnosis and treatment on newborn's incarcerated hernia.Methods:The clinical data of 34 cases of newborn's incarcerated hernia was analysed from 1999 to 2006.Results:32 cases of newborn's incarcerated hernia were treated by operation with 8 cases in intestinal necrosis and 5 cases in spermary necrosis.2 cases were treated by hands.There are 8 cases with complications and 2 dead cases post-operation.The cure rate of operation,complication rate and mortality were 94.11%,22.52% and 5.88%respectively.Conclusions:The early diagnosis and operation treatment is value for newborn's incarcerated hernia.Nutritional supportive treatment and preventing toxic shock are elevation curative effect criticality.

Objective To report 44 cases of meta-superior position anorectal malformation in different age therapied by posterior sagittal anorectoplasty(PSARP) operation, and to discuss possibility of one-stage anorectoplasty via posterior sagittal approach in neonate. Methods The clinical data of 44 cases meta-superior position anorectal malformation,which were treated by PSARP,were studied retrospectively. Results Of 44 cases, 15 were treated by colon stoma in neonate, then PSARP was executed after 3～7 months. And the rest 29 cases (9 in neonate and 20 in 2 months～9 years) were operated with one-stage anorectoplasty via posterior sagittal approach. Among 21 children followed from post-operration 6 months to 5 years, 18 cases of defecation clinical score were fineness. Conclusion The curative effects of PSARP in neonate to meta-superior position anorectal malformation is satisfactory. If the neonate is in good condition, one-stage anorectoplasty via posterior sagittal approach could be executed in infant, and favourable defecate function could be remain.

Objective To investigate the effects and complications of injection with triamcinolone acetonide acetate and Pingyangmycin on maxillofacial and perineal hemangioma. Methods Local injection with triamcinolone acetonide acetate (TAA) and Pingyangmycin (PYM) was performed to treat infant maxillofacial and perineal hemangiomas: strawberry hemangioma, cavernous hemangioma and mixed hemangioma, in 107 infants admitted to our hospital from 1996 to 2005. If the lesion did not disappear after the first treatment, the injection would be performed again every four to six weeks interval, and the total dose of TAA and PYM could not be more than 100 mg and 40 mg respectively. The injection treatment was repeated 4 times for one course and the therapeutic effects on maxillofacial and perineal hemangioma was evaluated. Results All cases were followed up for one year after local injection of TAA and PYM. The cure rate was 87.8%, the effective rate was 9.3%, and the ineffective rate was 2.8%. Conclusion The injection of TAA and PYM is a reliable and less side effect therapeutic method, and valuable for infant maxillofacial and perineal hemangioma.

Objective To investigate dynamic changes and clinical significance of the high-sensitive Creactive protein(hs-CRP) level in acute cerebral infarction patients with different etiological types.Methods 136 patients with acute cerebral infarction were recruited.These patients were classified into five subtypes based on Trial of Org 10172 in Acute Stroke Treatment(TOAST) criteria.Serum hs-CRP levels on the 1st,3rd,5th,7th,14th day after onset from patients and 42 healthy controls were measured with immunoturbidimetry.The neurologic impairmentscore was determined with NIHSS.Results Serum hs-CRP levels is higher on the 1th,3rd,5th,7th,14th day than that of the control group ( (4.26 ± 1.31 ),( 12.57 ± 6.29 ),( 10.23 ± 4.49 ),(7.54 ± 2.33 ),(4.25 ± 1.77) mg/Land (2.56 ± 0.86) mg/L,t = 7.89,10.26,10.99,13.55,5.97,P ＜ 0.05 ).Among 5 subtypes,serum hs-CRP was the highest in large-artery atherosclerosis group after acute ischemic stroke,and cardioembolism group was the next.Serum hs-CRP reached the highest on three or five days after disease onset and decreased slowly.High levels of hs-CRP in large-artery atherosclerosis group indicated severe neurologic functional impairment and worsen prognosis.Conclusions ACI is closely related to serum hs-CRP level,which can be used as an subjective index for severity and prognosis with the lasting,high levels of hs-CRP levels predict poor prognosis.

Faced with the development of medical computer information and lacking of medical students’ information education, integrated education of medical computer information capacity is explored and implemented. Medical students’ entire cognition on computer information is improved, their working and learning ability and efficiency are improved obviously by education, and thus medical students’ comprehensive ability is enhanced effectively.

Objective To study the expression of CD27 and CD28 in antigen-specific CD4~+T cells in patients with pulmonary tuberculosis and healthy people, and understand the role of differentiated stages of CD4~+T cells in the pathogenesis of tuberculosis. Methods The expression of CD27 and CD28 was analyzed by CD4, CD154, CD27 and CD28 staining and flow cytometry. The distributions of CD27 and CD28 in antigen-specific CD4~+T cells were compared between patients with pulmonary tuberculosis and healthy controls. Results In patients of pulmonary tuberculosis, the frequencies of CD27 + CD28 + (early differentiated stage), CD27~- CD28~+ and CD27~+ CD28~- (intermediate differentiated stage), CD27~- CD28~-(fully differentiated stage) T cell subsets in antigen specific CD4~+T cells were (49. 55 ±6. 15)%, (26. 85 ±3. 87)% ,(7. 2 ± 1.37)% and ( 16. 35 ±3.97)%, respectively. In healthy controls, the frequencies of the four subsets in antigen-specific CD4~+T cells were ( 51.81 ± 4. 94 ) %, ( 29. 83 ± 5.33 ) %, ( 12. 65 ±4. 48)% and (5.7±2)%, respectively. The early differentiated CD4~+T cell was the major subset both in patients and healthy people, however, which had significant difference compared with the fully differentiated subset ( t = 2. 26, P < 0. 05 ). Conclusion The population frequency of the fully differentiated CD4~+T cells in patients with pulmonary tuberculosis was significantly higher than that in healthy people. This suggested that the differentiation degree of the antigen-specific CD4~+T cell might be related with pulmonary tuberculosis.

Objective To study population frequencies of CD4+,CD154+ T cell subset in patients with pulmonary tuberculosis and controls with positive PPD reaction. Methods Flow cytometry was used to detect the CD4+,CD154+ T cell subset, the population frequen-cies in patients with pulmonary tuberculosis and controls were compared. Results The expression level of CD154 was higher when PE-la-beled CD154 antibody was added during stimulation period, compared with CD154 labeling after stimulation(1.51±0. 36/0. 40±0. 13, P <0.05). The CD154+ cells were not detectable in fresh isolated CD4+ T cells, but significantly increased after stimulation with specific anti-gens. The population of CD4+, CD154+ T cell subset was significantly reduced in patients with active pulmonary tuberculosis, compared with healthy controls with PPD positive reaction(0. 72±0. 32/1.65±0. 76, P <0. 01). Conclusions The population of CD4± ,CD154± T cell subset was significantly reduced in patients with active pulmonary tuberculosis, which indicated that it may play an important role in the de-velopment of tuberculosis.

Objective Idiopathic pulmonary fibrosis ( IPF) is a chronic inflammatory disease with unknown etiology and is lack of effective therapy. The aim of this study is to explore the function of Ca2+ and PI3K/AKT/mTOR pathway in the pathogenesis of IPF, and the impact of 1,25?( OH) 2 D3 on Ca2+ and PI3K/AKT/mTOR pathway in type Ⅱalveolar epithelial cells of rat with IPF. Methods 150 male SD rats were randomly divided into 2 groups: prevention group ( control groupⅠ, model groupⅠ, medication groupⅠ) and treatment group ( control groupⅡ, model groupⅡ, medication groupⅡ) . The tracheal exposure surgery was operated in control groupⅠ/Ⅱ, and then 200μL sterile physiological saline was administered by intraperitoneal injection of each rats 2 days and 14 days after surgery, separately. Bleomycin(BLM)(5 mg/kg) was in?jected into the trachea of model groupⅠ/Ⅱ, and then vitamin D3 solvent(0.1%ethanol and 99.9%glycol propylene, 1μL/g) was ad?ministered by intraperitoneal injection 2 days and 14 days after surger?y, separately. Bleomycin( BLM) ( 5 mg/kg) was injected into the tra?chea of medication groupⅠ/Ⅱ, and then 1,25?( OH) 2 D3( 2μg/kg) was administered by intraperitoneal injection 2 days and 14 days after surgery, separately. IPF model was built by injecting Bleomycin into the trachea of rats, 1,25?(OH)2D3(2μg/kg) was used to prevent and treat IPF by intraperitoneal injection in medication group. The hydroxyproline content of lung tissue in each group was measured, type Ⅱalveolar epithelial cells were separated from lung tissue and labeled with Fluo?3AM, then concentration of Ca2+ was detected by Laser scanning confocal microscope. The mRNA levels of PI3K, AKT and mTOR in the typeⅡalveolar epithelial cells were tested by RT?PCR. Results Compared with control groupⅠ/Ⅱ at each time point, hydroxyproline content of lung tissue, Ca2+ concentration and expression of PI3K, AKT and mTOR in typeⅡalveolar epithelial cells in model groupⅠ/Ⅱand medication groupⅠ/Ⅱwere sig?nificantly raised( P<0.05 or P<0.01) , but these were significantly reduced in medication groupⅠ/Ⅱcompared with model groupⅠ/Ⅱ( P<0.05 or P<0.01) . Correlation analysis showed that there is significant positive correlation between Ca2+ concentration and mRNA expression levels of PI3K, AKT and mTOR in model groupⅠ/Ⅱ(r=0.5988, r=0.6230, r=0.6603,P<0.01)and medication groupⅠ/Ⅱ( r=0.701 2, r=0.632 3,r=0.740 3,P<0.01) . Conclusion The PI3K/AKT/mTOR pathway plays an important role in devel?opment of IPF. 1,25?( OH) 2 D3 is able to reduce Ca2+concentration in typeⅡalveolar epithelial cells and inhibit the PI3K/AKT/mTOR pathway, and then inhibit the development of IPF.

Structure of biliary system is complex as well as various, making troubles for optimal surgical treatment of biliary disease. Remarkable imaging of biliary system helps surgeon evaluating patients and planning surgeries. There are several methods to obtain accurate anatomical information of biliary system, such as X-ray fluoroscopy, MRI and fluorescence-based imaging. Each has its own advantages and disadvantages. Combination of multi-model imaging technologies may improve visual result of anatomical information of biliary tract. More resolvable, legible, and sequential imaging technology of biliary system remains further study. This article reviews various cholangiography methods widely used in the clinical setting.