Objective To study the characteristics of heart rate variabili ty (HRV) in states of calm supine position, supine position and counting, and ca lm upstanding position, and the relationship with TCM syndromes of cold-h eat and deficiency-excess. Methods Totally 233 patient s diagnosed by TCM & western medicine with different syndromes were divided into five groups. B y comparing the general changes of frequency domain of HRV under three kinds of condition s, the variation of HRV indexes was measured in the excess heat syndrome group ( 64 cases), deficiency cold syndrome group (16 cases), cold-heat complex syndrom e group (38 cases), yin deficiency with fire syndrome group (67 cases), and defic iency of both yin and yang syndrome group (48 cases). Results The low frequenc y (LF) and low frequency normalized unit (LFNU) manifested sympathetic nervous a ctivity were augmented and were related with the yang syndrome and heat syndrome obvi ously. The high frequency (HF) and high frequency normalized unit (HFNU) manifes ted parasympathetic nervous activity were augmented and were related with the yi n syn drome and cold syndrome. The increased LF/HF reflected the yang syndrome and hea t syndrome. The decreased LF/HF reflected yin syndrome and cold syndrome. The co ndition of counting had a weighing influence on the diagnosis of yang syndrome a nd heat syndrome. And the condition of upstanding played a weighing role in defi ciency syndrome and cold syndrome. Conclusion The law o f changes of HRV under three kinds of condition is helpful in syndrome differentiation of cold-heat an d deficiency-excess.

Objettive To evaluate the effect of taking vitrectomy on the patients who suffer from polypoidal choroidal vasculopathy (PCV) combined with vitreous hemorrhage (VH),observe the natural consequence of PCV combined with VH and analyze the factors that affected the visual prognosis.Methods The clinical data of 20 patients with PCV combined with VH who performed vitrectomy were analyzed retrospectively from January 2009 to September 2012.Best corrected visual acuity (BCVA) was compared before and after operation,and explored the factors that affected the visual prognosis.Results Patients were followed up 6-18 (13 ± 2) months.At the last follow-up,BCVA was improved and better than 0.05 in 11eyes.There was significant difference in BCVA between before operation (2.91 ±0.52) and 2 weeks after operation (1.52 ±0.51)(P =0.003).The natural factors between visual acuity improved patients and non-improved patients were compared,follow-up treatment and the condition of macular was the independent factor that affected the visual prognosis (P =0.009,0.034).There was significant difference in BCVA of 6months after operation between patients with follow-up treatment (photedynamic therapy or transpupillary thermotherapy,10 cases) and patients without follow-up treatment (10 cases)(0.77 ± 0.51 vs.1.43 ± 0.64)(P =0.035).Conclusions Vitrectomy can significantly improve the visual acuity of PCV combined with VH.It will get better visual acuity,if has photodynamic therapy or transpupillary thermotherapy postoperatively.

This article sorts out and analyzes acupuncture-moxibustion items inTreatise on Cold Damage DiseasesandSynopsis of Prescriptions of the Golden Chamberand proposes regularities in application of and the characteristic ofZhangZhong-jing’s acupuncture and moxibustion. Acupuncture was often used for unblocking and regulating meridional qi and clearing and purging excessive heat. Moxibustion was often used for warming yang and remedying deficiency or dispersing and unblocking yang qi. Because of convenient operation, acupuncture-moxibustion therapy was often used to treatdangerous and serious diseases. Acupuncture and moxibustion were often combined with herbal prescriptions and other therapeutic methods to improve therapeutic effects, especially on difficult and complicated diseases.ZhangZhong-jing’s guiding thought ofacupuncture-moxibustion treatment first advocates treatment of diseases from the root and meanwhile, pays attention to treatment of both symptoms and causes of diseases. Secondarily, it emphasizes acupuncture-moxibustion treatment based on syndrome differentiation and especially values pulse condition, meridians and acupuncture points. It has abundant contents in treating the same disease with different methods and often selects specific acupoints. Thirdly, it favours preventive treatment of diseases, andwhether prevention of diseases before they arise or prevention of diseases from exacerbating was embodied in his acupuncture-moxibustion treatment.

Total 150 patients with chronic pelvic pain syndrome were randomly divided into three groups with 50 cases in each group. Patients were treated with tamsulosin, Qianlie'an Shuan and tamsulosin plus Qianlie'an Shuan for 12 weeks, respectively. Patients were assessed with the National Institutes of Health-Chronic Prostatitis Symptom Index (NIH-CPSI) before and after treatment. The score of NIH-CPSI decreased from 25. 1 ± 2. 2 to 19. 5 ± 1.7 after treatment in tamsulosin group; 25.0 ± 2. 2 to 19. 1 ± 1.7 in Qianlie'an Shuan group, and 24. 8 ± 1.7 to 16. 8 ± 1.4 in combined therapy group, all P ＜0. 01. The results indicated that tamsulosin, Qianlie' an Shuan or combined therapy were all effective in alleviating the symptoms and improving the quality of life, and the combined therapy was superior to single therapy in relief of symptoms.

It is necessary to medical student to take the elective course of pharmacy.This paper put forward several viewpoints and concrete methods about teaching methods,means and contents of elective course of pharmacy.

Objective To investigate the keratin 9 gene mutation in epidermolytic palmoplantar keratoderma (EPPK) and its relationship with clinical manifestations. Methods Three Chinese pedigrees with EPPK were studied. Polymerase chain reaction (PCR) was performed to amplify the seven exons encoded by keratin 9. Denaturing high-performance liquid chromatography (DHPLC), DNA sequencing and allele-specific PCR were used to reveal the sequence variation in the PCR products. Results An insertion-deletion mutation in the exon 1 of keratin 9 497delAinsGGCT, was revealed in all 3 EPPK families, resulting in the keratin 9 change from tyrosine166 to tryptophan and leucine (Y166delinsWL). Allele-specific PCR confirmed that the mutation was not a commonly seen polymorphism, but a novel mutation which has not been reported in The Human Intermediate Filament Mutation Database (http://www.interfil.org). Conclusions A new keratin 9 gene mutation, 497delAinsGGCT, is found in these Chinese EPPK pedigrees, which may be the genetic basis of EPPK.

Objective To observe the effects of intervention of soothing liver and activating blood Chinese medicine on cardiac function and myocardial pathologic morphology of BMSCs transplanting on myocardial IRI of rats, and investigate its myocardial protection mechanism. Methods Model of myocardial IRI was established by coronary artery ligation in rats. SD rats were randomly divided into sham operation group, IRI group, BMSCs group and combined group. Rats in combined group were filled the stomach with soothing liver and activating blood Chinese medicine, and rats in other groups were filled the stomach with the same dose of normal saline. After 4 weeks, myocardial pathologic morphology was observed with light microscope. Cardiac function was detected with ultrasonic cardiogram.Results Compared with BMSCs group, heart function of the combined group improved, with significant statistical difference (P<0.05,P<0.01). Pathological observation showed that myocardial structure and pathological morphology were obviously promoted in the combined group.Conclusion Soothing liver and activating blood Chinese medicine could improve heart function and myocardial pathological morphology of IRI rats with BMSCs transplantation.

Objective To determine the baseline echocardiographic characteristics and the time course and degree of recovery of left ventricular (LV) systolic dysfunction in patients with tachycardia-induced cardiomyopathy ( TCM ) . Methods Seven hundred and fifteen patients received radiofrequency cather ablation ( RFCA ) for tachycardiarrhymias from July 2010 to July 2013 were screened in this study. Only 33 patients with reduced left ventricular ejection fraction (LVEF) (LVEF<50% and improved≥15%) were diagnosed with tachycardia-induced cardiomyopathy and were included in the study. Patients with early improvement (over 25%increase in LVEF at 1-week follow-up compared to the baseline ) were enrolled in the improved group , and the rest patients were enrolled in the improved group. All Patients received transthoracic echocardiography for LV size and function detection at 1 week and at 3,6,12 months follow-up. Results The average baseline of the LV end-diastolic diameter, and the LVEF were (55 ± 10.7)mm and (38 ± 4.6)%, repectively. Early improvement ( over 25%increase in LVEF at 1-week follow-up compared to the baseline ) in the improved group was observed in 16 patients. Patients with early improvement had higher LVEF at 12-month follow-up compared to the patients without early improvement [(69.2± 4.2)% vs (58.1 ± 6.9)%, P < 0.001]. Conclusions RFCA is proved to be a relatively safe and effecient treatment method. Atrial fibrillation related to TCM , rhythm control is superior to the rate control. The early improvement in LVEF may potentially predict the complete reversibility of LV systolic dysfunction.

Objective To analyse the rate of coincidence to suspected coronary artery disease (CAD) patient between positive exercise treadmill testing (TET) and coronary angiography (CAG) test.Method Two hundred and sixty-eight chest pain patients positive in TET were performed CAG test,coronary stenosis exceed 50% was diagnosed CAD. Results One hundred and seventy-six patients (65.67%) was diagnosed by CAG, 105 male and 71 female in them. Ninety-two (34.33%) patients was negative in CAG test. The false positive rate of TET between post-menopause patients (30.12%, 25/83 ) andmale patients (28.08%, 41/146) was no significant difference(P > 0.05 ); the difference of false positive rate between pro-menopause patients (66.67%, 26/39) and male patients (28.08% ,41/146) was statistical significance (P < 0.01 ). Conclusions The positive rate of CAG can be greatly improved by screening the chest pain patients though TET before CAG, if the positive in TET performed CAG only. TET false positive rate of pro-menopause female is significantly higher than post-menopause female or male.

Objective:To observe the efficacy of calcined bovine bone(CBB)in the repair of animal bone defects.Methods:Ca-nine alveolar bone defect model,rat and rabbit calvarial critical size defect models were established respectively.All animals were randomly divided into 2 groups(n=6).The defects in the experimental group were repaire with CBB,those in the control group were not treated.The effects were observed by HE staining,Micro CT and Masson trichrome staining.Results:Canin alveolar bone de-fects were well repaired 8 week after operation.The skull defects in rabbits and SD rats were replaced by new bone 1 2 and 8 weeks after operation respectively.In all the control groups bone defects were not healed or not completely repaired.Conclusion:CBB is ef-fective in the repair of bone defects.