After acute myocardial infarction, how realizes revasculadzation of the infarct area as well as prolongs the postponement left ventricle reconstruction and restores heart normal contraction function has been the hot spot. The research demonstrated that the transplanted stem cells may be in field planting of the heart infarct region, simultaneously through nutrition effects of secreting vascular endothelial cells and differentiation into myocardial cells, vascular endothelial calls, and can improve ventricle reconstitution and recovery cardiac function. However, stem call effects on myocardial infarction have close correlation to stem call tissue source. Various preconditioning also can affect repair of stem cells. Meanwhile, choice of suitable stem cell donors and recipients, correction of transplanted window and pathway are key factors for obtaining good clinical curative effects.

Objective: In order to study how to accelerate the reconstitution immunofunction in BMT mice, first of all, we established a immunodeficiency model of BMT in BALB/C mice. Then BMT mice were injected with PCD-hIL-2 directly into skeletal muscle, and treated with traditional Chinese medicine. Methods: The experiment groups are designed as（A）Chinese medicine + PCD-hIL-2;（B）PCD-hIL-2;（C）Chinese medicine +hIL-2;（D）Chinese medicine;（E）hIL-2;（F）BMT;（G）normal control;（H）radiation control. Results: We compared groups A B C D to E or F groups, found（1）The splenocytes/thymocytes count increase obviously.（2）Killing activity of NK cell rises obviously in vivo.（3）The response of splenocytes、thymocytes、BM cells to mitogen goes up.（4）The reactivity of splenocytes to foreign IL-2 goes up. （5）CFU-GM count is increased. Conclusion: The expression of hIL-2 is very low by nude DNA injection ,but it is enough to have biological function and therapeutic effect .If only Chinese medicine was applied, the immunological condition was obviously recovered.

0.05). No procedure related complications and malignant arrhythmia were found during 6-12 months follow-up. Conclusion Intracoronary transplantation of ABMMNCs is safe and may improve the heart function.

Objective To investigate the effect of donors' activating killer cell immunoglobulin-like receptor (aKIR) on recipients prognosis post haemopoietic stem cell transplantation (HSCT).Methods Fifty-nine cases of related HLA full matched HSCT were studied. Sequence specific primer polymerase chain reaction (SSP-PCR) was used to type donors' KIR. Virus,bacterium,and fungi infection as well as bleeding,relapse,survival were observed in the recipients post transplantation. Relationship between aKIR and clinical indexes mentioned above were analyzed.Results Donors' aKIR showed no beneficial effects on recipients' virus and bacterium infection. Furthermore,the incidence of fungi infection was increased in HSCT if donors expressed KIR3DS1 (?2= 4.804 ,P= 0.028 ). No significant difference was found in survival curve between donor aKIR positive HSCT or negative one (KIR2DS1+/-: P= 0.651 ; KIR2DS2+/-: P= 0.847 ; KIR3DS1+/-: P= 0.341 ). No effect of donor's aKIR on relapse was observed,too.Conclusion In Guangdong Han,so far as related HLA full matched HSCT is concerned,no improvement in recipient prognosis due to donor's aKIR is verified.

Objectives To determine the changes of right ventricular hemodynamic in the early phase after lung resection and non-lung resection thoracotomy by Doppler echocardiography and to investigate the correlation with preoperative lung function.Methods 55 patients who underwent thoracotomy were divided into two groups. In group A: 15 patients underwent non-lung resection thoracotomy; in group B: 40 patients underwent lung resection. The group B were subdivided into group B1 (n=23) and group B2 (n=17), acording to the preoperative lung function results: Artery blood gas analysis were measured and right ventricular hemodynamic indices were calculated by Doppler echocardiography before and after operation,respectively.Results Right ventricular ejection fraction (RVEF) and artery oxygen pressure(PaO 2) significantly decreased (P

Objective:To study methods for inducing CD34+ cells of human bone marrow to differentiate into T cells in vitro to provide theory and method basics for the investigation of activity of T cells derived from psoriatic bone marrow CD34+ cells and establish a technological platform to investigate T lymphopoiesis activity of hematopoietic cells.Methods:Bone marrow CD34+ hematopoietic progenitor cells were isolated by immunomagnetic cell selection and induced differentiate into T cells in the bone in the marrow and thymic stromal microenvironment.Immunfluorescence dying method and flow cytometry analysis were performed to detect CD1-CD3+ cells,CD3+CD4+CD8-cells and CD3+CD4-CD8+ cells dynamically.Results:In the first week,the non-adhension cells were composed mostly of immature CD1+CD3-cells and CD1+CD3+ cells and small proportion of mature CD1-CD3+ cells.In the following analysis,the proportion of immature cells rapidly decreased and CD1-CD3+ cells increased.After 1 week culture,CD4+CD8+ double positive T cells and a small population CD4+CD8-and CD4-CD8+ could be detected among the CD3+ cells.In the following culture,the proportion of CD4+CD8+ double positive T cells decreased significantly and single positive T cells increased gradually.However,small proportion of mature T cells could be detected in the early stage and cann't be found after 4 weeks in the culture system without thymic stromal cells.Conclusion:Mature single positive T cells can develop from CD34+ hematopoietic progenitor cells in the bone marrow and thymic stromal microenvironment and the thymic stromal cells are vital for T lymphopoiesis.

Objective:To compare the seeding efficiency and graft versus host disease (GVHD) of severe combined immunodeficient (SCID) mice transplanted with human hematopoietic cells intraperitoneally (ip) and intravenously (iv) and to explore the method to set up psoriatic animal model by xenogeneic bone marrow transplantation.Methods:Normal human bone marrow mononuclear cells (BMMNC) were separated by density gradient centrifugation and BMMNC (4?10~7) were injected into lethally irradiated SCID mice by ip or iv injection. GVHD symptom and periphery blood white blood cell resuming dynamics in mice were observed after xenotransplantation and flow cytometry was performed to detect human source CD45~+ cells proportion in periphery blood and bone marrow of mice.Results:The mice transplanted by tail intravenous injection presented obvious GVHD symptoms promptly 2 weeks after transplanting and only one mouse survived in 12 weeks. Among the mice received tail intravenous injection and dealed with cydosporin(CsA) and methotrexate(MTX),some of the mice showed slight GVHD symptom and survival rate was 80%(8/10) in 12 weeks. Slight GVHD symptoms appeared after human bone marrow transplantation by intraperitoneal injection and then most of mice returned to the normal and the survival rate was 70%(7/10) in 12 weeks. The periphery blood white blood cells resuming dynamics, CD45~+ cell proportion of periphery blood and bone marrow after transplantation show no significant difference between the groups transplanted by intravenous and intraperitoneal injection.Conclusion:Human hematopoietic cells could home to bone marrow in SCID mice and result in hematopoietic reconstitution. The transplantation method by intraperitoneal injection, which showed efficient seeding capability, can be used to both bone-marrow transplantation and reducing GVHD.

Objective To study the influence of culture supernatant of psoriatic peripheral blood mononuclear cells (PBMCs) on the colony-forming of bone marrow-derived hematopoietic stem cells and progenitor cells. Methods Bone marrow-derived mononuclear cells were separated by density gradient centrifugation. Methylcellulose semi-solid culture medium was used to culture the bone marrow mononuclear cells in culture systems. The PBMC culture supernatant from psoriatic patients or normal controls were added to the culture, to observe their influence on the marrow-derived high proliferative potential colony forming cells (HPP-CFCs), erythroid and granulocyte-macrophage colony forming units (CFUs-E and CFUs-GM) of bone marrow hematopoietic stem/progenitor cells from healthy individuals. Results The values of HPP-CFCs, CFUs-E and CFUs-GM were significantly lower in the bone marrow cells stimulated by the supernatant of cultured psoriatic PBMCs than those stimulated by the supernatant of cultured normal PBMCs and those in the spontaneous proliferation group (P 0.05). Conclusions Psoriatic PBMCs have specific biological activity and can inhibit the colony forming of bone marrow hematopoietic cells from healthy individuals.

Objective To study the in vitro activity of T cells differentiated from bone marrow CD34+ cells of psoriatic patients with family histories. Methods Bone marrow CD34+ cells were isolated from psoriatic patients with family history and normal persons by immunomagnetic cell selection and induced to differentiate to T cells in thymic stromal microenvironment in vitro. CD3+ T cells were obtained by CD3+ cell selection system and the proportions of CD4+CD8- and CD4-CD8+ cells were measured by flow cytometry. The proliferation activity of T cells was measured by MTT colorimetry in both spontaneous proliferating group and superantigen (SAg)-stimulated group. And the levels of IL-4, IL-8 and IFN-? were quantified by enzyme-linked immunosorbent assay in culture supernatant. Results Mature CD4+CD8- cells and CD4-CD8+ cells were detected from CD3+ cells, which were derived from bone marrow CD34+ cells. When the psoriatic patients and normal controls were compared, there was no significant difference in the proportion of CD4+CD8- cells or that of CD4-CD8+ cells in CD3+ cells. The proliferation activity of T cells was significantly higher in the psoriatic patients than that in the normal controls. There was no statistical difference of IL-4?IL-8 or IFN-? levels in the supernatant of T cells between the spontaneous group and the normal control. However, the IFN-? and IL-8 levels in the supernatant of T cells were higher in the psoriatic patients than those in the normal controls after the SAg stimulation. Conclusion The abnormal activity of peripheral blood T cells from psoriatic patients with family history may be related to the bone marrow hematopoietic cells.

To investigate the clinicpathologic features and the main point of differential diagnosis of T-cell lymphomas in breast. MethodsTwo cases of T-cell lymphomas of the breast were analysed about clinic data, histopathology and imrnunochemistry. ResultsBoth of the patients were women whose age were 37 and 31 years old. Histopathologically, the tumor cells appeared sheet and cord-like arrangements. Some of them distributed around the blood vessel. There was an obvious phenomenon closing blood vessel. Tumor ceils were characterized by less cytoplasm, big and distorted nucleus, thin chromation. Large pieces of necrosis were observed. There were no lympho-epithelial lesions. Immunnohistochemistry showed that CD45, CD45RO, CD3 and CD43 in the tumor cells were all positive, while CD20 and CD74 were negative. Follow-up results showed that one died two and half months after operation and the other died sixteen months after operation. ConclusionsT-cell lymphoma of the hreast is the high malignant tumur which is extremely rare, and its diagnosis mainly depends on the histopathology and the marker of immunohistochemistry.