Objective To explore the clinical efficacy of hysteroscopic IUD intrauterine surgery for incarcerated ring,and to assess the safety.Methods 586 women with the incarcerated dervice remove under hysteroscope were selected in the hospital (study group),693 women during the same period used conventional methods of taking the dervice (control group ).The status of surgery,cervical relaxation,take the dervice and effect and safety were assessed.Results The operation time,amount of vaginal bleeding and take the dervice time of the study group were (19.36 ±3.14)min,(9.42 ±2.11)mL and (8.17 ±1.90)min,which were lower than those of the control group [(30.29 ±4.08)min,(15.67 ±2.38)mL and (11.35 ±2.26)min],the differences were statistically significant (t=13.16,11.51,9.76,P<0.05).The cervical relaxation goodand take part successfully of the study group were 490 cases(83.62%)and 435 cases(74.23%),which were higher than those of the control group[374 cases (53.54%)and 362 cases(52.24%)],the differences were statistically significant (χ2 =10.28,11.35,P<0.05). Waist lower abdominal pain (0-Ⅰ grade)and vaginal bleeding number (no vaginal flow or<1 day)of the study group were 509 cases (86.86%)and 362 cases (61.77%),which of the control group were 527 cases (76.05%) and 362 (46.23%),and the differences were statistically significant (χ2 =9.94,10.23,P<0.05 ).Conclusion Hysteroscopic IUD intrauterine surgery can shorten the operation time and take the dervice time,reduce the amount of vaginal bleeding and bleeding time.It can improve the success rate of taking the dervice and has fewer negative symptoms.It has high safety and clinical advantages are obvious.

Objective To calculate the single dose of traditional or modified trastuzumab to avoid mistakes during the course of drug making up.Methods Based on clearly relationship among the multiple parameters for the trastuzumab preparation,the trastuzumab bottle label is generated by means of the Excel form.Results There was no clinical case of using durgs errors happened during the course using among 280 patients.Conclusions The trastuzumab bottle label by means of the Excel form could improve the accuracy and safety of clinical use of trastuzumab.

The female sheep reproductive tracts were freshly collected from a local meat processing plant and used as experimental materials. Two antibacterial peptides were isolated and characterized from female sheep reproductive tracts by two consecutive chromatographic steps. The peptide isolation procedures included acetic acid extraction, dialyzed, gel filtration chromatography on Sephadex G-50, and reverse phase high-performance liquid chromatography (RP-HPLC). Their molecular mass were 4 820.47 u and 4 012.5 u, respectively, analyzed by MALDI-TOF-MS. The partial N-terminal amino acid sequences of two peptides were determined as AYVLDEPKP and YDSGA, respectively, by Edman degradation. The antimicrobial activity was tested during each purification step by the radial diffusion plate assay and broth microdilution method. These two peptides showed good antimicrobial activities against reference strains of G~+(S. Aureus ATCC2592 and Streptococcu ATCC55121), G~-(E. Coli ATCC25922) and fungi(C. Albicans ATCC2002). The peptides did not show active hemolytic activity against rabbit blood red cells and had no significant effects on human blood coagulation system. The discovery of antibacterial peptides in sheep reproductive system reveals that antibacterial peptides may play a role in innate immunity against microorganisms in a wide range of animal species.

Chemistry Techniques, Analytical ; instrumentation ; Herbicides ; analysis ; Humans ; Paraquat ; analysis

OBJECTIVE: To seek the bionic material myocardial extracellular matrix in the myocardial tissue engineering for the cultured myocardial cells and to provide a reliable scaffold.METHODS: A computer-based online search of CNKI and PubMed database to search articles published between January 1996 and August 2007 on scaffold material of tissue engineering.RESULTS: Natural biological scaffold materials with good biocompatibility and mechanical properties are ideal choice in the cardiac tissue engineering rapid prototyping technology. Natural biological materials such as collagen, chitosan have poor mechanical properties and plasticity, but good biocompatibility, which can be used for rapid prototyping of cardiac stents. The scaffolds which are prepared by the technology such as three-dimensional printing, laser sintering, three-dimensional pressing,selective laser sintering rapid prototyping technology on have the advantages such as high porosity, high surface area volume ratio,completely transparent among the holes, controllable macro-shape, porosity, and independent control of aperture.CONCLUSION: Through the use of dispersion/accumulation forming principle, surface modification of traditional biological materials in cardiac tissue engineering using computers and other high-tech nano-polymer technology is expected to provide ideal myocardial scaffolds.

Objective To evaluate the influence of SP600125,a selective c-Jun N-terminal kinase (JNK) inhibitor,on the levels of serum total bile salt (TBA) and Bsep,Ntcp expression in the hepatic tissue of rats with ethinylestradiol induced intrahepatic cholestasis.Methods Rats pregnant for 15days were administered the subcutaneous injection of 17- b -estradiol propylene ( EE ) to modulate the ICP animal models,and be SP600125 to intervene.Testing the level of serum TBA and the expression of c-Jun,Bsep,Ntcp in the hepalatic tissue.Results The average gray values of c-Jun in the group of ICP models were significantly lower than the normal control group ( 101.05 ± 5.20 vs 118.99 ± 5.95,P ＜ 0.05 ).After the intervention of SP600125,comparing with the group of ICP models,the expression of Bsep,Ntcp in the group of SP600125 intervention were significantly higher,and this change in the high dose of SP600125 intervention group was more obvious ( low dose intervention group Bsep:0.452 ±0.031 vs 0.291 ±0.043,Ntcp:0.462 ± 0.015 vs 0.285 ± 0.021,P ＜ 0.05 ; high dose intervention group Bsep:0.568 ± 0.038 vs 0.291 ±0.043,Ntcp:0.605±0.020 vs 0.285 ±0.021,P ＜0.05),while the level of TBA in the serum was significantly lower.Conclusions Treatment with SP600125 can down-regulate the level of c-Jun/AP-1,and it may participate in the lower expression of Bsep、Ntcp in the ICP rats which were induced by 17-bestradiol.

With the development of the socialist new rural reconstruction,the contradiction between the demand for medical health care and the insufficiency in hygienic resources in the countryside is increasingly prominent. Training the practical medicine talented person who are willing to go to the countryside and are able to stay there and be used for the countryside community has already become the important mission for the medicine higher professional training school. Taking my school’s actual situation as the example,we make exploration on orientting accurately,deepen medical education reform,establish and raise practical talented persons with skills.

Practical teaching is an important component of higher medical education. The article introduced a number of exploration and experience of Shaoyang Medical College in building a practical teaching quality guarantee system,such as revising personnel training programs, optimizing practice teaching environment,enhancing teaching quality construction projects, strengthening teachers troop construction reinforcing teaching practice management and the teaching quality monitoring,etc.

0.05). No procedure related complications and malignant arrhythmia were found during 6-12 months follow-up. Conclusion Intracoronary transplantation of ABMMNCs is safe and may improve the heart function.

Objective To investigate the hemostasis effect of alginate dressing for puncture point bleeding after peripherally inserted central catheter (PICC) by ultrasound-guided modified Seldinger technique (MST).Methods Between November 2013 and May 2016, 538 cases of cancer in our department underwent PICC, including 270 cases from November 2013 to February 2015 selected as control group (after PICC was successfully placed, sterile gauze and 3M transparent film were used to fix the puncture point) and 268 cases from March 2015 to May 2016 selected as observation group (after PICC was successfully placed, alginate dressing, sterile gauze and 3M transparent film were used to fix the puncture point).The patients in both groups were given 20 minutes of pressure for puncture point and elastic bandage compression fixation for 24 h.The degree of puncture point bleeding and times of dressing change in 24 h and 24-72 h between the two groups were compared.Results In the observation group, mild, moderate, and severe bleeding was seen in 232 cases, 34 cases, and 2 cases within 24 h, and in 242 cases, 25 cases, and 1 case between 24-72 h, respectively.In the control group, mild, moderate, and severe bleeding was seen in 12 cases, 196 cases, and 62 cases within 24 h, and in 21 cases, 212 cases, and 37 cases between 24-72 h, respectively, with significant differences (Z=-18.647, P=0.000;Z=-18.768, P=0.000).In the observation group, there were zero, once, twice, and ≥3 times of puncture point dressing change in 232 cases, 33 cases, 3 cases, and 0 case within 24 h and in 242 cases, 25 cases, 1 case, and 0 case between 24-72 h, respectively.In the control group, there were zero, once, twice, and ≥3 times of puncture point dressing change in 12 cases, 196 cases, 51 cases, and 11 cases within 24 h and in 21 cases, 209 cases, 37 cases, and 3 cases between 24-72 h, respectively, with significant differences (Z=-18.560, P=0.000;Z=-18.755, P=0.000).Conclusion The hemostasis effect of alginate dressing for puncture point bleeding after ultrasound-guided PICC by MST is satisfactory.