OBJECTIVE:To keep abreast of the situation and trend of patent Chinese materia medica used in our hospital METHODS:To collect the amount of consumption and sum of money of patent Chinese materia medica used in our hospital in 2000～2001 and to analyse the frequency of drug use RESULTS:The consumption of part of drugs decreased by 7%～17% Those drugs with increasing tendency in consumption kept stable in increase The DDDs of compound red sage tablet increased by 22% CONCLUSION:For supply and management of patent Chinese materia medica,it is very important to analyse and understand the sum of money of consumption and frequency of drug use

AIM To prepare D-α-tocopherol polyethylene glycol 1000 succinate (TPGS)-modified artesunate liposomes and to investigate the in vitro anti-tumor activity.METHODS The liposomes prepared by thin-film dispersion method were characterized by transmission electron microscopy and particle size analyzer,and the encapsulation efficiency was determined by ultrafiltration centrifugation.The liposomes' cytotoxicity to human hepatoma HepG2 cells was evaluated by MTT method.RESULTS The average particle size,PDI,Zeta potential,encapsulation efficiency,drug loading of the liposomes were 126.7 nm,0.182,-10.1 mV,78.8％ and 18.38％,respectively.The liposomes displayed a significant inhibition on HepG2 cells with the IC50 value of 0.034 μmol/mL.CONCLUSION Compared with non-TPGS-modified artesunate liposomes,the TPGS-modified artesunate liposomes prepared by this method afford smaller vesicle size,better stability and higher encapsulation efficiency with stronger in vitro anti-tumor activity.

Objective To verify the clinical applicability of the published standard intervals of routine clinical chemistry (WS/T404 .1‐2012 ,WS/T404 .2‐2012) based on the health examination results of Han and Uygur populations in Urumqi .Methods This was a retrospective study .The results of serum TP ,ALB ,ALT ,AST ,ALP ,GGT from healthy examination individuals of Han and Uygur populations (from 2013 August to 2015 January) were collected and the healthy cases (age range:20 -79 years old) were chosen to calculate the 2 .5% and 97 .5% percentiles ,excluding the significant abnormal results according to the Medical Deciding Level 2 recommended by Staland .The percents of health cases not falling in the published standard interval were calculated to meet the judgment criterion of verification (<10% ) .Results The test of normality revealed that the Han and Uygur's results of all veri‐fied items were skewed distributions .The 2 .5% and 97 .5% percentiles of the results of two populations were as follows ,TP(Han 65 -81 g/L ;Uygur 64-81 g/L) ,ALB(Han 41-53 g/L ;Uygur 40-52 g/L) ,ALT(Han:male 9-51 U/L and female 7-42 U/L ;Uygur:male 9-53 U/L and female 6-43 U/L) ,AST(Han:male 14-42 U/L and female 12-37 U/L ,Uygur:male 12-42 U/L and female 12-38 U/L) ,ALP(Han:male 45-119 U/L ;Uygur:male 47-122 U/L) ,ALP(female 20-49 years old:Han 35-95 U/L and Uygur 40-104 U/L) ,ALP(female 50-79 years old:Han 43-131 U/L and Uygur 51-132 U/L) ,GGT(Han:male 11-71 U/L and female 8-54 U/L ;Uygur :male 11 -73 U/L and female 7 -55 U/L ) .The percents of AST results for Han's male , Uygur's male and Uygur's female not falling in the published standard reference interval were slightly over 10% ,but AST results o‐ver 10% were mainly under the lower limit of the published standard reference interval .The health case percents for the other veri‐fied items of Han and Uygur populations not falling in the published standard reference interval were under 10% .Conclusion The published standard reference intervals of routine clinical chemistry (WS/T404 .1‐2012 ,WS/T404 .2‐2012) are applicable in our la‐boratory for the detection of Han and Uygur population .

Objective To provide the objective evidence for reducing stat test turnaround time (TAT) reasonably through the comparative analysis of different intervals of stat test TAT between emergency department (ED) and intensive care unit (ICU ) . Methods Laboratory information system was used to collect data about blood cell analysis and biochemical profiles of department of emergency and ICU from 1st January to 31th March ,2014 ,then comparatively analyzing different intervals of stat test TAT be‐tween two departments .Results TAT outlier rates of stat CBC tests ordered by ED and ICU were 2 .4% and 15 .1% ,and that of stat biochemical profiles ordered by ED and ICU were 12 .3% and 24 .5% ,respectively .there were no significant differences in mean times between order‐to‐receipt of stat CBC tests and biochemical profiles ordered by ED and collection‐to‐receipt of stat CBC tests and biochemical profiles ordered by ICU [(11 .2 ± 4 .0) min vs .(11 .2 ± 4 .5) min ,P>0 .05 ;(13 .2 ± 14 .1)min vs .(13 .8 ± 9 .8) min ,P>0 .05] .ED had significantly shorter mean time of receipt‐to‐report than ICU for stat CBC tests and biochemical profile [(5 .8 ± 4 .4) min vs .(19 .3 ± 12 .5) min ,P<0 .01 ;(34 .4 ± 10 .9) min vs .(35 .5 ± 13 .2) min ,P>0 .01] .The TAT mean times of stat CBC tests and biochemical profiles ordered by ED were shorter than those ordered by ICU [(17 .0 ± 6 .2) min vs .(30 .5 ± 14 .9) min ,P<0 .01 ;(46 .9 ± 17 .2) min vs .(49 .3 ± 16 .5) min ,P<0 .01] .Conclusion The ED TATs for CBC tests and biochemical pro‐files are reasonably set ,and each interval of the ED TATs is well controlled .The ICU TATs for CBCs and biochemical profiles should be reset ,and the process of stat test for ICU should be optimized .

Objective To investigate the values of ultrasound measuring of the placenta thickness on evaluation of risks for alpha-thalassemia at the early pregnancy.Methods Two-dimensional ultrasound was performed to measure the thickness of placenta on 208 cases of fetuses with alpha-thalassemia and 52 cases of normal fetuses in control group.The placenta thickness was expressed as multiples of the median(MOM).Results At the early pregnancy,the group of fetuses with alpha-thalassemia had significantly higher placenta thickness compared to the fetuses without alpha-thalassemia(P＜0.001).However,there were no statistical significant difference in the placenta thickness between the other groups(P=0.100).Placenta thickness 1.18 MOM was the best critical point to predict alpha-thalassemia.The sensitivity and specificity of placenta thickness >1.18 MOM in prediction of alpha-thalassemia was 82.9%,84.7% respectively.Conclusions For those with high risks of alpha-thalassemia placenta thickness measuring is a safe,effective parameter for assessment because it could reduce unnecessary invasive procedures and improve the detecting rate of severe alpha-thalassemia.

Objective:To investigate the relationship between rs5896 polymorphism of prothrombin gene and renal calculi in the elderly patients.Methods:Ninety elderly patients with kidney stones admitted to Hangzhou Hospital of Zhejiang Medical and Health Group from January 2014 to December 2016 were selected as observation group, and another 80 healthy people during the same period were selected as control group.The prothrombin gene rs5896 polymorphism was detected by RT-PCR, and the differences between the two groups were compared.Results:The T frequency of rs5896 allele in the observation group (64.44%) was higher than that in the control group (46.25%) (χ 2=5.687), and the TT genotype (54.44%) was higher than that in the control group (38.75%) (χ 2=4.187, P<0.05). TT genotype (77.78%) at rs5896 locus was more than single stone (38.89%), and CC genotype (16.67%) was less than single stone (37.04%) (χ 2=13.171, 4.363, all P<0.05). The serum uric acid level of TT type[(434.21±78.13)μmol/L] was higher than that of CC+ CT type[(245.73±54.20)μmol/L] ( t=13.038, P<0.05). The serum creatinine, calcium, phosphorus and potassium levels of different genotypes had not statistically significant differents (all P>0.05). The urine level of uric acid in TT-type [(415.32±56.63)μmol/L] was higher than that in CC+ CT type [(267.31±67.18)μmol/L] ( t=11.343, P<0.05), but there were no statistically significant differences in the changes of calcium, phosphorus and potassium in different genotypes of urine (all P>0.05). Conclusion:The T gene frequency of prothrombin gene rs5896 is higher and TT genotype frequency is higher in elderly patients with renal calculi.It is suggested that T gene may be a susceptible gene and that this site may lead to renal calculi through uric acid metabolic disorder, which is worthy of clinical reference.

Objective:To study the effect of cyclin Y (CCNY) on the lesion degree of patients with liver cancer and its correlation with preoperative and postoperative liver injury.Methods:The clinical data of 60 patients with liver cancer (liver cancer group) and 69 patients with liver cirrhosis (liver cirrhosis group) in Hangzhou Hospital of Zhejiang Medical and Health Group from January 2015 to October 2017 were retrospectively analyzed. In liver cirrhosis group, Child-Pugh liver function grade A was in 33 cases (liver cirrhosis grade A group), grade B was in 21 cases (liver cirrhosis grade B group), grade C was in 15 cases (liver cirrhosis grade C group). The serum total bilirubin (TBIL), alanine aminotransferase (ALT), albumin, cholinesterase, γ-glutamyl transpeptidase (GGT) and total bile acid were detected by automatic biochemical analyzer. The serum CCNY was detected by WB method, and compared with 40 healthy subjects (healthy control group).Results:Compared with those in healthy control group, the albumin and cholinesterase in liver cirrhosis grade A, B and C groups were significantly decreased, the ALT, TBIL, GGT, total bile acid and CCNY were significantly increased, , and the changes were more obvious with the severity of liver disease, and there were statistical differences ( P<0.05). Pearson correlation analysis result showed that the CCNY was positive correlation with TBIL, ALT, total bile acid and GGT in patients with liver cancer ( r = 0.544, 0.612, 0.553 and 0.539; P<0.05), and CCNY was negative correlation with albumin and cholinesterase ( r = - 0.478 and - 0.620, P<0.05). In patients with liver cancer, before operation and 1, 2 and 7 d after operation, the CCNY was 3.01 ± 1.10, 7.24 ± 2.57, 6.29 ± 1.78 and 4.01 ± 1.52, ALT was (98.74 ± 16.79), (430.55 ± 197.62), (255.73 ± 26.77) and (121.89 ± 20.42) U/L, respectively; the CCNY and ALT 1 and 2 d after operation were significantly higher than those before operation, those 2 d after operation were significantly lower than those 1 d after operation, those 7 d after operation were significantly lower than those 2 d after operation, and there were statistical differences ( P<0.05); there were no statistical difference between 7 d after operation and before operation ( P>0.05). The expression of CCNY before operation and 1 d, 2 d, 7 d after operation was positive correlation with ALT in patients with liver cancer ( r = 0.669, 0.821, 0.663 and 0.642; P<0.01). Conclusions:The more severe the degree of liver lesions in patients with liver cancer, the higher the serum CCNY, and the higher the expression of CCNY, the more severe the degree of liver injury in patients with liver cancer due to surgery, which is positively correlated with liver injury indexes.

Objective To improve the study on chronic obstructive pulmonary disease (COPD) basicknowledge, and the ability of COPD research and design, organization, implementation andmanagement. Methods Clinical research led stu-dents to participate in the COPD, found the problem from clinical,research students went to COPD, literature, grouping the recent 15 years , the discussion of the COPD design experimental study , lipopolysaccharide ( LPS ) rat model of COPDinduced with smoke, explore the love Luo Ning on the treatment of cough with dyspneaeffect COPD and its mech-anism, integration of traditional Chinese and Western medicineknowledge module to the pathogenesis of COPD, as the core of the problem of the cause. Results 5 graduate students completed COPD in the etiology, pathogenesis andmech-anism of Kechuanning love Luo, master and technology related research methods. Conclusion PBL teaching method helps to promote the quality of postgraduate education,PBL teaching was worth to promoted.

Objective To investigate the effect of standardized extended care on the patients after endoscopic sinus surgery .Methods Eighty cases after endoscopic sinus surgery , from January 2012 to January 2013 , were randomly divided into treatment group and control group , with 40 cases each .The control group was taken the usual care and discharge guidance , and the treatment group was given the standardized extended care . Results The effective rate of the treatment group and the control group were 97.5% and 85.0%.There was significant difference between groups (χ2 =2.23,P <0.05).2 cases in the treatment group occurred nasal adhesions, with a complication rate of 5.0%.In the control group, 6 cases occurred nasal adhesions;1 case occurred maxillary sinus stenosis; 1 case occurred orbital hemorrhage , with a complication rate of 20.0%. There was significant difference between groups (χ2 =3.45,P =0.561).Conclusions The standardized extended care can significantly improve patient treatment efficiency and reduce complications .It is worthy of clinical application .

Objective To explore the clinical efficacy of full-time nursing intervention on the treatment of sinusitis and nasal polyps through endoscopic .Methods One hundred and ten patients with sinusitis and nasal polyps from October 2009 to June 2012 in our hospital were chosen and divided into the control group and the observation group according to the random number table , each with 55 cases.All patients were all given the endoscopic surgery , and the control group received the routine nursing , and the observation group received the full-time nursing intervention before and after the operation on the basis of the traditional nursing .The clinical efficacy was observed in two groups , and the incidence rate of postoperative complication was compared between two groups, and the psychological state of patients was analyzed by self-rating anxiety scale ( SAS) and self-rating depression scale ( SDS ) .Results The total effective rate was 96 .4% ( 53/55 ) in the observation group, and was higher than 83.6%(46/55) in the control group, and the difference was statistically significant (χ2 =11.23,P<0.05).The incidence rate of complication was 5.5%in the observation group, and was lower than 14.5%in the control group, and the difference was statistically significant (P<0.05).The score of SAS in the observation group was (41.7 ±4.23) after intervention, and was better than (63.8 ±3.75) before intervention, and the score of SDS was (40.6 ±4.10) after intervention, and was better than (62.5 ±3.99) before intervention;the score of SAS in the control group was (55.8 ±3.92) after intervention, and was better than (64.1 ±4.04) before intervention, and the score of SDS was (56.2 ±4.11) after intervention, and was better than (62.8 ±4.13) before intervention.The scores of SAS and SDS after intervention were respectively (41.7 ±4.23), (40.6 ±4.10) in the observation group, and were better than (55.8 ±3.92), (56.2 ±4.11) in the control group, and the differences were statistically significant ( t =10.70,11.49, respectively;P <0.05 ) .Conclusions Full-time nursing intervention can significantly improve the clinical efficacy of endoscopic treatment of sinusitis and nasal polyps , and reduce the occurrence of complication , and relieve the anxiety and depression of patients , and is worthy of clinical promotion .