OBJECTIVESpecies containing extremely aromatic compounds in leaves of Chimonanthus was analyzed to evaluate its genetic diversity and genetic relationships.

METHODAFLP molecular marker technique was used in the study, UPGMA cluster analysis was conducted with the software of POPGENE32.

RESULTSFive hundred and fifty-nine bands were amplified by 10 pairs of primers screened, of which 226 bands were polymorphic, and the percentage of polymorphic bands was 36.8%. Observed number of alleles, effective number of alleles, Nei's genetic diversity index and Shannon's information index were 1.992 6, 1.306 5, 0.199 2 and 0.325 1, respectively. Genetic distances of the 21 populations were ranged from 0.039 2 to 0.289 4.

CONCLUSIONSpecies containing extremely aromatic compounds in leaves of Chimonanthus with low genetic diversity play an important role in enhancing the protection of species and germplasm resources. Form the molecular level, the studies demonstrated the correctness of the result by Zhang Ruohui that species containing extremely aromatic compounds in leaves of Chimonanthus were divided into Ch. salicifolius, Ch. Zhejiangensis, Ch. nitens and Ch. grammatus.

Amplified Fragment Length Polymorphism Analysis ; Calycanthaceae ; classification ; genetics ; DNA, Plant ; genetics ; Genetic Variation ; genetics

OBJECTIVETo reveal the mutative discipline of the contents of the phenols in samples from different provenances.

METHODThe contents of magnolol and honokiol in samples from the filial generation of Guanxi, Hubei and Zhejiang provinces were determined respectively by HPLC.

RESULTThere were significant variations between provenances, the contents of honokiol, magnolol and phenols were the highest in samples from Hubei province, those were the lowest in samples from Zhejiang province. There were not only significant differences in polyphenol content between provenances, but also in families in the same provenance and individuals in families.

CONCLUSIONMagnolia polyphenol content and appearance traits were significantly related. The type with the small convex tip (original form) were superior to other types of M. officinalis judging from the characteristics of tree classification. The high quality of M. officinalis should actually be attributed to their local varieties and the breeding strategy of M. officinalis should be paid full attention to the good provenances, good families and good individuals.

Magnolia ; chemistry ; Phenols ; analysis ; Plant Extracts ; analysis