Objective:Based on the national standard of Classification and Codes for Fixed Assets(GB/T 14885),it created a catalogue of the fixed assets specific for hospital,which became a tool for the delicacy management of the fixed assets in hospitals.Methods:Based on the compiling principles and application scope,it conducted comparative analysis between the national standard and the catalogue which was created by the First Affiliated Hospital with Nanjing Medical University.Results:The amounts of the categories were significantly less than that of national standard.Among them,the categories of medical equipment and furniture were clearly more than the national standard.Conclusion:The effect of the national standard was limited in the delicacy management of the fixed assets in hospitals.However,the specific catalogue helped to uniform the value management and material management,to support information system of fixed assets in hospitals and enhance the level of delicacy management of the fixed assets in hospitals.

Objective To explore the diagnostic value of color Doppler ultrasonography and ultrasonography in the differential diagnosis of ectopic pregnancy.Methods 142 patients with ectopic pregnancy were given ultrasonic examination,to observe whether intrauterine gestational sac,carefully looking for recognition of ovarian masses and distinguish the relationship and structure,and were compared with pathologic diagnosis.Results Ultrasound could clearly display the uterus,ovary,failed to break the gestational sac and blood(block) mass image structure,with pathological diagnosis coincidence rate was 95.1％ (135/142),the misdiagnosis rate was 1.4％ (2/142),the missed diagnosis rate of 3.5％ (5/142).Conclusion Ultrasound was rapid,simple,noninvasive,high-accuracy,it was the preferred method for early diagnosis of the ectopic pregnancy.

Objective:To analyze and evaluate population health informatization development of China. Meth-ods:Through this investigation, 8 578 copies of a questionnaire designed for all levels of health administration and parts of primary hospitals were collected. These collected data, were analyzed to evaluate the population health infor-matization development of China through the evaluation index system established for population health informatization development and comprehensive evaluation model. Results:The results of this empirical study indicate that the popu-lation &Health Informatization overall has rapid development , but still has regional differences, and total score of the top 10 provinces are Zhejiang, Shanghai, Jiangsu, Liaoning, Beijing, Chongqing, Tianjin, Hunan, Henan and Gan-su. A statistical difference (p<0. 05) exists in regional development on population & health informatization of Chi-na, therefore serval provinces have obvious weakness or advantage in the construction of informatization. Discussion& suggestions:Government of China should pay attention to evaluation during the construction of informatization;pro-mote a balanced and sustainable development by strengthening financial support and optimizing the input pattern;up-grade regional population health informatization overall according to the information resources as the core;and develop the key priorities of population health informatization depending on practical situation and developing trend.

Objective To investigate the effect of dipeptidyl peptidase-4 (DPP-4) inhibitor on lipopolysaccharide (LPS)-induced changes in the mass and function of pancreatic β-cells.Methods RINm cells were cultured and treated with LPS alone or combined with different concentrations of sitagliptin for 24 h.The proliferation of RINm cells was detected by CCK-8 assay.Apoptotic rate was determined by Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide flow cytometry.Insulin secretion was measured by enzyme-linked immunosorbent assay.The expression of IL-6 mRNA was displayed by RT-PCR.Results LPS significantly stimulated the proliferation of RINm cells (0.89 ± 0.04 vs 1.14 ± 0.08,P＜0.01),while LPS+sitagliptin showed no significant difference compared with LPS group.The cell apoptotic rate in LPS + 10-1 mmol/L sitagliptin group was significantly lower than that in LPS group.There were no significant differences in basal insulin secretion among all groups,but after the high/low glucose stimulation,LPS increased insulin secretion as compared with the control.The IL-6 mRNA expression in LPS+sitagliptin group was significantly lower than that in LPS group (0.77 ± 0.33 vs 1.30 ± 0.41,P =0.006).Conclusions DPP-4 inhibitor has no influence on LPS-induced proliferation of pancreatic β-cell,but it can inhibit LPS-induced apoptosis and insulin secretion,and IL-6 may be involved in the process.

Objective To investigate the protecting mechanism of heat stress pretreatment on acute lung injury(ALI). Methods The oleic acid ALI mouse model was built to dynamically observe the binding capacity and the binding affinity of glucocorticoid receptor(GR),the levels of GR,heat shock protein 90(Hsp90)and Hsp70 before and after hyperthermic stress pretreatment. Results Heat stress pretreatment had significant protective effect on ALI.Western blotting showed insignificant changes of GR levels but progressive increase of level of Hsp70 and Hsp90.Heat stress pretreatment exerted insignificant effect on Bmax and Kd of GR,shown by radio ligand binding assay after ALI. Conclusion The protective effects of heat stress pretreatment on ALI of mouse may relate to its ability of keeping stable GR level and increasing levels of Hsp70 and Hsp90.

ObjectiveTo investigate the mechanism of dexamethasone (Dex) in inhibiting monocyte adhesion and phagocytose function.Methods Under the stimulation of phorbo1-12-myristate-13-acetate (PMA),U937 monocytes cultured in vitro were treated with Dex and Fasudil respectively.The adhesion rate of U937 monocles to human umbilical vein endothelial cells (HUVECs) and their phagocytic ability of India ink were studied.The protein content and activity of rho-associated coiled-coil protein kinase 1 ( ROCK1 ) as well as the effects of mifepristone and cycloheximide on Dex were determined.ResultsBoth DEX and Fasudil could significantly inhibit the adhesion tate and phagocytosis of U937 cells stimulated by PMA and suppressed the activity of ROCK1.While mifepristone and cycloheximide could not alter these effects of DEX.ConclusionDEX interferes with the adhesion and phagocytosis function of U937 cells by inhibiting ROCKI activity.

Objective: To compare the prevalence and genotype distribution of human papillomavirus (HPV) in female cervix and vulva. Methods: Gene chip technique was applied to detect HPV and determine HPV genotypes in 251 female patients with cervical and vulvar exfoliated cell samples. Chi-square test using SPSS was used to analyze the differences between the two sites. Results: Out of the 251 patients, 121 (48.21%) had the same HPV test result in the cervix and vulva, and 130 patients (51.79%) had different test result . Among them, the cases who were HPV-positive in the cervical and vulvar region were 128 and 149, and the positive detection rates were 51.00% and 59.36%. In addition, the prevalence rates of HPV and high-risk HPV were significantly higher in vulva than cervix (262 vs. 206, 199 vs.154, all P<0.05). While the prevalence rates of low-risk HPV was not significantly higher in vulva than cervix (63 vs. 52, P=0.765). All the 21 HPV genotypes were detected in both regions. The most common low-risk types were type 6 and type 81, while the top four genotypes of high-risk were 16, 52, 51 and 58. The infection rates of two or more HPV genotypes were significantly higher in vulva than cervix (21.52% vs. 29.88%, P=0.032). Conclusions Due to the close correlation between the cervix and vulva in anatomy and the high HPV detection rate in the vulva, it is suggested that in clinical work, we should not only pay attention to the HPV infection of the cervix, but also pay more attention to the vulva.

A multiplex PCR method was developed to detect the main pathogens of Qinghai Tibetan sheep endometritis. First, the genomes of five standard bacterial strains were extracted and specific primers were selected; the multiplex PCR method was established by using the genome of the standard strain as a template. The samples were collected by sterile cotton swab from Tibetan sheep uterus, and then placed in LB medium and numbered. After 48 h, the genomes of cultured bacteria were extracted and detected by single PCR method, then the positive samples were recorded. The positive samples detected by single PCR were selected for multiplex PCR detection and recorded again. The coincidence rate between these two methods was calculated to measure the accuracy of multiplex PCR. In order to identify the species of the pathogen, 30 positive samples verified by single and multiplex PCR were randomly selected for bacterial isolation and identification. In the 600 samples, the infected ratio of Streptococcus agalactiae (GBS) was 47.33%, Escherichia coli 34.83%, Staphylococcus aureus 6.5%, Salmonella and Trueperella pyogenes were negatively detected. Among the positive samples detected by multiplex PCR, the positive ratio of GBS was 45.50%, E. coli 33.50%, S. aureus 6.5%. Comparison of two detection results, Multiplex PCR detection coincidence rate is more than 95%. The isolated pathogens were identified as E. coli, GBS and S. aureus, which was consistent with the results of two methods. The multiplex PCR method was successfully established and the main pathogens of endometritis in Qinghai Tibetan sheep were GBS, E. coli and S. aureus.
Animals ; Bacteria ; genetics ; isolation & purification ; Bacteriological Techniques ; methods ; Endometritis ; microbiology ; veterinary ; Female ; Multiplex Polymerase Chain Reaction ; standards ; Polymerase Chain Reaction ; veterinary ; Sensitivity and Specificity ; Sheep ; Sheep Diseases ; microbiology ; Tibet