Survivin,the smallest and structurally unique member of the inhibitor of apoptosis protein (IAP)family,is highly expressed during fetal development and rarely expressed in self -renewable healthy adult tissues.Moreover,it exists in most malignant tissues .Survivin is described as a bifunctional protein involving in the regulation of cell proliferation and apoptosis in normal and cancer cells .Due to its structural and functional distinctiveness ,survivin is currently attracting considerable attention as a new target for cancer molecular targeted therapy.Strategies to targeting Survivin include antisense oligonucletides ,ribozymes,siRNA,dominant negative mutants,small molecule antagonists and immunotherapy .

A new closed bipolar electrode electrochemiluminescence ( ECL)-based device was designed, and further used to investigate the ECL behaviors of luminol in this device. Our results showed that, while a suitable voltage was applied to the two poles of the closed bipolar electrode, both the positively charged ions and luminol-based anionic ions could be enriched on the two poles of the closed bipolar electrode, respectively. More importantly, the ECL signals, generated from the electro-oxidation of luminol on anodic pole, were found to be related to the total amount of positively charged ions on the cathodic pole of the closed bipolar electrode. Under the optimum experimental conditions, the ECL response was linearly to the concentration of analyte in the range of 1. 0×10-9-1. 0×10-8 mol/L with a detecting limit of 1. 1×10-10 mol/L. Based on this finding, a new ECL method for sensing the solution conductance was developed.

Objective To evaluate the value of diagnose of colon carcinoma by colonoscopy and double contrast radiography.Methods The results and data of radiography diagnosis,colonscopy diagnosis,biopsy and post-operation pathology were analyzed retrospectively.Results The rate of accordance by X-ray diagnosis was 85.4%,colonscopy 80.5% and biopsy 84.6%.Conclusion The method of examination of colon double contrast radiography preceeds to colonoscopy examination when the patients were suspected suffering from the right colon or left colon carcinoma accompanying by masses;colonoscopy is the first method of diagnosis to choose when the patients were suspected suffering from carcinoma of sigmoid colon or colon polyps undergoing malignant changes.

Objective To explore CT diagnostic values of cardiac carcinoma.Methods CT findings of cardiac cancer comfired pathologically in 50 cases were analysed.All case were examined with spiral CT.Results The thickness of gastric cardia wall was found in all 50 cases,soft tissue masses was found in 25 cases,adjacent tissue invasion was found in 18 cases and metastasis of lymph nodes was found in 33 cases.Conclusion CT is a valuable method in demonstrating the size of cardiac carcinoma,its adjacent tissue infiltration and lymph node metastasis.It plays important role in the planning of treatment in clinical practice.

Objective To explore the characteristics and applied value of diffusion-weighted MR imaging of kidney in healthy people.Methods Using Philips Gyroscan Intera 1.5T MR System,DWI of kidney was performed in 40 healthy adult volunteers,the ADCs of renal cortex and medulla were measured.Results The cortex and medulla of the normal kidney could be clearly showed by DWI.The ADCs of the cortex medulla and average were 3.08?0.52,2.74?0.68 and 2.91?0.58;2.79?0.51,2.53?0.65 and 2.63?0.62;2.64?0.43,2.32?0.47 and 2.48?0.44 when the b values were 300,500 and 800 s/mm2,respectively.The ADC was higher in the cortex than that in the medulla.Conclusion DWI can be used in kidney,it may be helpful for the diagnosis of renal diseases.

Objective To study the applied value of MR diffusion-weighted imaging(DWI)and apparent diffusion coefficient(ADC)value in chronic kidney disease(CKD).Methods 10 healthy volunteers and 45 patients with different period of CKD underwent routine MRI and DWI.The DWI features were observed.The ADC values were acquired by using Philips soft package on the ADC images.Results Under different b value(300 s/mm2,500 s/mm2 and 800 s/mm2),the ADC value of CKD were 2.29?0.27,2.07?0.21 and 1.91?0.22,respectively,which were significantly lower than that in normal kidneys,and there was statistical difference between different group of CKD.Conclusion DWI may be a noninvasive method to evaluate the renal function.

Objective To evaluate the value of helical CT and pulmonary artery angiography in the diagnosis and treatment of pulmonary embolism. Methods The data of helical CT and pulmonary angiography of 18 cases of pulmonary embolism suspected by clinical diagnosis were analyzed retrospectively. Pulmonary embolism was diagnosed in 12 patients with pulmonary angiography, and treated by suction throngh cathether and local fibrinolysis. Results Embolism was found to involve 107 pulmonary artery branches in the 18 cases, including bilateral pulmonary lower lobes in 28.97%, left and right main pulmonary artery in 22.43%, lobar artery and segmental artery in 40.18%, and main pulmonary artery in 8.41%. The direct signs of helical CT and pulmonary angiography included partial filling defect, mural filling defect, central filling defect or so-called railway-track sign, and total occlusion. Secondary signs included enlargement of pulmonary artery trunk, local pulmonary digemia, pulmonary infarction and pleural effusion. 12 patients survived and showed clinical improvement with significant increase in PaO_2. Conclusion Helical CT is a noninvasive and effective method for the diagnosis of pulmonary embolism, while the pulmonary artery angiography is also an important tool in diagnosis and treatment of it.

Objective To analyze the imaging features of mycobacterium in AIDSpatients.Methods Twenty-three cases of mycobacterium tuberculosis and 13 patients of non-tuberculous mycobacteria were proved etiologically and included in this study.All patients underwent X-ray and CT examinations,imaging data were analyzed and compared.Results The imaging findings of mycobacterium tuberculosis in AIDS patients included consolidation (n =11),pleural effusion (n =11),mediastinal lymphadenopathy (n =11).Pulmonary lesions were always diffuse distribution,and 14 patients of extrapulmonary tuberculosis were found.Pulmonary lesions in non-tuberculous mycobacteria tend to be circumscribed.Conclusions Non-tuberculous mycobacterial infection in AIDS patients is more common and usually combined with other infections.Imaging features are atypical.

Objective To study the CT manifestations and diagnostic value of stromal tumor.Methods CT documentation of 25 stromal tumor cases confirmed by surgery and pathology were analyzed retrospectively,all cases underwent 64 row or 256 row spiral CT plain scan and enhanced scan.Results Within 25 cases,1 7 cases were gastrointestinal,of which,10 cases were found in stom-ach,1 case in duodenum,3 cases in jejunum and 3 cases in ileum;and 8 cases were extra-gastrointestinal,of which,6 cases were found in enterocoelia,1 cases in inguinal region,1 case in abdominal wall;the ratio was 2.1 ︰ 1.In 25 cases,highly malignant dan-ger level were 1 1 cases,7 cases of moderate and 7 cases of low malignant danger;Transfer occurred in 7 cases and 3 cases of postop-erative recurrence.Conclusion The volume CT has great advantages in displaying information in lesion,and has high value in loca-ting the lesion and qualitative diagnosis of disease.

Objective To improve recognition and diagnosis of primary non-obstructive megaureter.Methods The authors analyzed the X-ray findings of intravenous urography and B-ultrasonographic manifestations of 28 cases with primary non-obstructive megaureter proved by varied examinations and operations.Results The X-ray and B-ultrasonographic manifestations as follows:in the terminal ureter,there was a short segment,less than 3 cm in length,with normal caliber but adynamic to micturition and the ureter proximal to the adynamic segment was secondarily dilated remarkably.The terminal end of the dilated ureter appeared as drumstick shaped,spindle-shaped,snakehead shaped or rattail shaped.On right time fluoroscopy and B-ultrasonographic observation,a decrease of peristalsis frequency,increase of peristalsis range,interrupted downward convey of peristalsis wave could be noted.Conclusion The contrast urography is the main method,B-ultrasonography and cyctocopy are helpful for the diagnosis of primary non-obstructive megaureter.