Based on the AuNPs/Nafion composite membrane technology and immobilization of amino adenosine aptamer using carboxyl carbon nanotubes on the surface of a glassy carbon electrode, a electrochemiluminescence sensor was preparated.The sensor was characterized by cyclic voltammetry and electrochemical luminescence.The result showed that the sensor had a good stability and reproducibility.Adenosine and adenosine aptamer could form G-tetrahedral structure, leading a decrease of ECL intensity.Under the optimum experimental conditions, the relative ECL intensity showed a good linear relationship to the negative logarithm of adenosine concentration in the range of 1.0×10-11-1.0×10-7 mol/L, the linear equations was ΔIECL=-890lgC-5050 with a detection limit of 5.0×10-12 mol/L.The RSD was 2.7% in 11 times measurement of adenosine (1.0×10-10 mol/L).The recovery was 97.1%-110.0% in the determination of real adenosine sample.

Objective To investigate the value of combinedevaluation of serum lipoprotein associated phospholipase A2 (LP-PLA2) and cystatin C (CysC) on systemic lupus erythematosus (SLE) patients with lupus erythematosus.Methods A total of 177 patients with SLE were enrolled in the General Hospital of People's Liberation Army from June 2000 to February 2017,87 cases in lupus group (SLE group),90 cases in lupus nephritis group (LN group),60 cases in healthy control group.The concentration of LP-PLA2 and CysC were measured.The ROC curves was established for estimation of the cutoff values of two indexes in SLE patients with LN,and the diagnostic efficacy of different diagnostic criteria was compared.Results The LP-PLA2 and CysC concentration in the LN group were higher than those in the SLE group (Z=-2.512,-5.688,all P＜0.05).The cutoff value of LP-PLA2 was 245.5 U/L and the cutoff value of CysC was 1.235 mg/L on the risk assessment of in SLE patients with LN.When using the parallel method for combined LP-PLA2 and CysC to evaluate the risk of LN,the sensitivity and negative predictive value would be significantly improved (x2 =9.461,4.377,all P＜ 0.05),the efficiency was better.Conclusion Parallel assessment of serum LP-PLA2 and CysC have clinical value on assessment of the risk of LN in SLE patients.

0 05), there had slight interference to low value serum and had no interference to high value serum by 125 mg/L Vitamin C, the correlation of UIBC reagent kit from different manufacturer was excellent( r = 0 994 4, n = 198) UIBC of serum samples from100 health blood donors (19 years old to 52 years old) were determined with UIBC reagent kit, and the reference result is 26 0-51 0 ?mol/L( ? 2 s ) Conclusion The UIBC result determined by this method is exact and reliable The UIBC reagent kit is easy to operate and ready for automated analyzer Then UIBC assay is worth to popularize

Objective To examine the predictive value of serum soluble E-selectin ( sE-selectin) levels for high risk plaques as detected by coronary computed tomography angiography ( CCTA).Methods This was a cross-sectional study.CCTA was performed for each patient.Serum levels of sE-Selectin, sVCAM-1,sICAM-1 and sP-Selectin were measured by flow fluorescence immunomicrobead assay ( FFIA). ROC curve and multivariate analysis were used to determine the predictive value for the presence of high risk plaques.Results The high risk plaques group showed higher sICAM-1 and sE-selectin levels.The multivariate Logistic analysis showed that male (OR＝2.131, P＝0.019), old age (OR＝1.053, P＝0.002), hyperlipidemia (OR＝1.960, P＝0.022), high level of apoB (OR＝2.896, P＝0.004) and high level of sE-Selectin(OR＝1.975, P＝0.008) were independently associated with the presence of high risk plaques.The AUC of the multivariate model for high risk plaques was 0.722.The sensitivity, specificity, the positive predictive value and the negative predictive value were 55.3%, 78.3%, 71.8% and 64.4%respectively.Conclusions High sE-selectin levels were associated with the presence of high risk plaques in asymptomatic populations at low to intermediate Framingham risk .sE-selectin can play an important role in plaque screening, as detected by coronary CTA.

Objective To investigate the effect of inhibition of Rabll expression on the proliferation and invasion of human bladder cancer cell line T24. Methods T24 cells were transfected with Rabll siRNA, and RNA interference efficiency was determined by performing Western blotting. The effect of inhibition of Rabll expression on cell proliferation, cell cycle progression, and cell invasion was analyzed by performing CCK8, cell cycle detection, and Matrigel invasion assays, respectively. Expression of cell cycle-related proteins cyclin D1 and cyclin E and invasion-related protein matrix metalloproteinase 9 (MMP9) was determined by performing Western blotting and RT-PCR. Results Inhibition of Rabl 1 expression inhibited the proliferation and invasion of bladder cancer cells and downregulated the expression of cell cycle-related proteins cyclin D1 and cyclin E and invasion-related protein MMP9. Conclusion Our results suggest that Rabl 1 functions as a tumor protein and is involved in the progression of bladder cancer.

Objective Toinvestigatestatistically significant peptide peaks as biomarkersto diagnose osteoarticular tuberculosis, matrix-assisted laser desorption/ ionization time of flight mass spectrometry (MALDI-TOF MS) was applied to identify the characteristic fingerprint among the serum of patients with osteoarticular tuberculosis, rheumatoid arthritis and healthy adults.Methods Clinical Study. Serum samples of untreatedpatients with osteoarticular tuberculosis and rheumatoid arthritis were collected from August 2018 to December 2018, and serum samples of healthy adults from physical examination were collected as control. After analysis with MALDI-TOF MS, the serum peptide fingerprint datawas imported into software, and protein polypeptide peaks with obvious differences were screened to establish diagnostic models.Results Established the diagnostic model of osteoarticular tuberculosis and healthy adults with m/z 2943.9, 5929.6, 7615.4 and 9033.8 as differential protein polypeptides, the diagnostic model of osteoarticular tuberculosis and rheumatoid arthritis with m/z 4195.6, 5847.6, 5929.6 and 7748.6 as differential protein polypeptides. To these two models, the sensitivity were 95.00％ and 97.50％, respectively. The specificity were 85.71％ and 88.46％, respectively. The accuracy rates were 89.58％ and 92.39％, respectively. The AUC value of ROC curves were 0.8859 and 0.8709, respectively. Conclusions By mass spectrometry and software analysis, the serum protein polypeptides with statistical difference were found successfully. The related diagnostic modelsarealso established, which has certain reference value for auxiliary diagnosis of osteoarticular tuberculosis.

Objective:To study the non-target metabolomics analysis and to analyze the metabolomic changesof cerebrospinal fluid (CSF) in patients with tuberculous meningitis.Methods:Case-control study. From July 2018 to July 2019, 20 cerebrospinal fluid specimens of diagnosed patients with tuberculous meningitis were collectedin the department of neurology from the first medical center of the PLA general hospital and the eighth medical center of the PLA general hospital and 20 CSF without tuberculous meningitis as the control. Among them, there were 12 males and 8 femalesin the tuberculous meningitis group, aged (37.9±16.1) years; there were 13 males and 7 femalesin the control group, aged (34.7±14.8) years. Using ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS) technology with three different mode, namely reverse phase chromatography positive ion mode, reverse phase chromatography negative ion mode and hydrophilic chromatography positive ion mode,to detectthe metabolic fingerprints of patients′CSF and analyzed by SIMCA software for orthogonal partial least squares discriminant analysis (OPLS-DA). The variable importance projection value of OPLS-DA model (threshold value>1) plus the P value of t-test (P<0.05) was applied to find the differential metabolites in the cerebrospinal fluid of the two groups of patients.Results:Ten differential metabolites were found in CSF, including L-isoleucine, L-phenylalanine, L-kynurenine, L-methionine, L-tyrosine acid, dimethylglycine, L-alanine, L-threonine, L-histidine and L-lysine, and all of them were up-regulated in the tuberculous meningitis group.Conclusion:Changesof the amino acid metabolism found in the cerebrospinal fluid of tuberculous meningitis patients can provide basis for differential diagnosis and basic molecular research of tuberculous meningitis.

Objective To evaluate the practice of peripheral blood eosinophil in the diagnosis of eosinophilic chronic rhinosinusitis (ECRS).Methods The correlation between eosinophil count and percentage in peripheral blood and that in topic tissue in 787 patients (the first affiliated hospital of Wenzhou medical university,from Jan.2013 to Jun.2016) with chronic rhinosinusitis (CRS) were retrospectively analysed.The optimal cutoff value of blood eosinophil count and percentage as predictors for ECRS was determined by receiver operating characteristic curves (ROC) and their diagnostic ability was compared.Results The positive correlation between eosinophil count and percentage in blood and that in tissue was found respectively in 787 patients with CRS (r =0.450,0.499,0.463,0.465,P ＜ 0.01).Although the significant correlation between blood eosinophil count and its count and percentage in tissue was not found after blood leukocyte and tissue eosinophilic inflammation was controlled respectively (r =0.041,P =0.380;r =0.046,P =0.329 and r =0.023,P=0.618;r =0.032,P =0.499),blood eosinophil percentage still showed significant correlation with tissue eosinophil count and percentage,but reduced unequally after that(r =0.383,0.436 and r =0.153,0.169,P ＜0.01).With ROC analysis,the diagnostic ability of optimal cut off values of eosinophil count and percentage varied as the histological criteria for ECRS differed.Conclusions Eosinophil in peripheral blood showed significant positive correlation with its tissue infiltration,which may be not strong and easily effected by individual factors.Theoretically,blood eosinophil may have a diagnostic significance as a predictor for ECRS but not practically.

BACKGROUND:Studies have shown that metformin has anti-inflammatory,anti-tumor,anti-aging and vasoprotective effects,and can inhibit the progression of osteoarthritis,but its specific mechanism of action remains unclear. OBJECTIVE:To investigate the mechanism of metformin on cartilage protection in a rat model of osteoarthritis. METHODS:Forty male Sprague-Dawley rats were randomly divided into four groups(n=10 per group):blank,control,sham-operated,and metformin groups.The blank group did not undergo any surgery.In the sham-operated group,the joint cavity was exposed.In the model group and the metformin group,the modified Hulth method was used to establish the osteoarthritis model.At 1 day after modeling,the rats in the metformin group were given 200 mg/kg/d metformin by gavage,and the model,blank,and sham-operated groups were given normal saline by gavage.Administration in each group was given for 4 weeks consecutively.Hematoxylin-eosin staining,toluidine blue staining,and safranin O-fast green staining were used to observe the morphological structure of rat knee joints.Immunohistochemical staining and western blot were used to detect the protein expression of SOX9,type Ⅱ collagen,a disintegrin and metalloproteinase with thrombospondin motifs 5(ADAMTS5),Beclin1,P62,phosphatidylinositol 3-kinase(PI3K),p-PI3K,protein kinase B(AKT),p-AKT,mammalian target of rapamycin(Mtor),and p-Mtor in rat cartilage tissue. RESULTS AND CONCLUSION:The results of hematoxylin-eosin,toluidine blue and safranin O-fast green staining showed smooth cartilage surface of the knee joints and normal histomorphology in the blank group and the sham-operated group,while in the model group,there was irregular cartilage surface of the knee joint and cartilage damage,with a decrease in the number of chondrocytes and the content of proteoglycans in the cartilage matrix.In the metformin group,there was a significant improvement in the damage to the structure of the cartilage in the knee joints of the rats,and the cartilage surface tended to be smooth,with an increase in the number of chondrocytes and the content of proteoglycans in the cartilage matrix.Immunohistochemistry staining and western blot results showed that compared with the control and sham-operated groups,the expression of SOX9,type Ⅱ collagen,and Beclin1 proteins in the cartilage tissue of rats in the model group was significantly decreased(P<0.05).Conversely,the expression of ADAMTS5,P62,as well as p-PI3K,p-AKT,and p-Mtor proteins was significantly increased(P<0.05).Furthermore,compared with the model group,the expression of SOX9,type Ⅱ collagen,and Beclin1 proteins in the cartilage tissue of rats in the metformin group was significantly increased(P<0.05),while the expression of ADAMTS5,P62,as well as p-PI3K,p-AKT,and p-Mtor proteins was significantly decreased(P<0.05).To conclude,Metformin can improve the autophagy activity of chondrocytes and reduce the degradation of cartilage matrix in osteoarthritis rats by inhibiting the activation of PI3K/AKT/Mtor signaling pathway,thus exerting a protective effect on articular cartilage.

COVID-19 is caused by a novel coronavirus-severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), which has being spreading around the world, posing a serious threat to human health and lives. Neutralizing antibodies and small molecule inhibitors for virus replication cycle are the main antiviral treatment for novel coronavirus recommended in China. To further promote the rational use of antiviral therapy in clinical practice, the National Center for Infectious Diseases (Beijing Ditan Hospital Capital Medical University and the First Affiliated Hospital, Zhejiang University School of Medicine) invited experts in fields of infectious diseases, respiratory and intensive care to develop an Expert Consensus on Antiviral Therapy of COVID-19 based on the Diagnosis and Treatment Guideline for COVID-19 ( trial version 10) and experiences in the diagnosis and treatment of COVID-19 in China. The consensus is concise, practical and highly operable, hopefully it would improve the understanding of antiviral therapy for clinicians and provide suggestions for standardized medication in treatment of COVID-19.