Objective To establish a HPLC method for determining the ferulic acid in Baidianfeng Pill. Method ODS column (4.6 mm?250 mm, 5 ?m) was used, the mobile phase consisted of methanol-1% acetic acid aqueous solution(1∶1). The flow rate was 1.0 mL/min and column temperature was at 30 ℃. The detecting wave length was at 320 nm. Results The method was linear with the range of 11.20～84.0 ?g/mL for ferulic acid (r =0.9 998). The average recovery was 98.45%. Conclusion The method is simple, rapid and accurate. It can be used for quantitative deternination of ferulic acid in Baidianfeng Pill.

Objective To examine the relationship between premature ventricular contraction(PVC) and microvascular leakage in the myocardium due to microvascular contrast echocardiography(MCE). Methods Thirty eight rats were randomized into 5 groups: control, ultrasound exposure only, contrast agent only, real time MCE and trigger imaging MCE. Anesthetized rats with tail vein catheter were imaged in a 37℃ water bath at left ventricular parasternal short axis view. Optison was injected at a dosage of 5 ml/kg. Frequency was 1.7 MHz, MI= 1.7 and the depth of image at 10 cm. Results PVC was detected in all ultrasound exposure groups, together with petechial hemorrhages and Evans Blue leakage in the scan band. The triggered imaging showed worse effects than real time imaging. No PVC or microvascular leakage was noticed in controls or sham exposed rats. Conclusions Induction of PVC during contrast added echocardiography is associated with microvascular leakage.

AIM:To investigate the effects of Fu Fang Xiao Chai Hu Tang(FFXCHT)on level of Interleukin-2 and value of CD4+/CD8+ in mice bearing Ehrlish ascites carcinoma(EAC).METHODS:The effects of FFXCHT on the EAC were observed and index of thymus and spleen were observed.The method of [3H]-TdR incorporation was used to measure the IL-2 level,and the value of CD4+/CD8+ was assayed by ELITE calibur flow cytometry.RESULTS:Compared with the model group,FFXCHT inhibited the growth of EAC(P