1.Study on Processing for Rhizoma Pinelliae (Processed with Licorice and Lime)by Orthogonal Design
Chinese Traditional Patent Medicine 1992;0(08):-
The procedure for Rhizoma Pinelliae(Processed with Licorice and Lime) was combinatively evaluated by L_9(3)~4 orthogonal design with three factors:decocting times for Radix Glycyrrhizae,concentrated volume of decoction and macerating time. The optimum condition is as follows: each 100g of Rhizoma Pinelliae,accompanied with 15g of Radix Glycyrrhizae (decocted with water for two times,then the two decoctions were combined,and concentrated to 150ml.)and 10g of calx,was macerated in concentrated decoction of Radix Glyeyrrhizae for six days
2.Simultaneous Content Determination of Quercetin and Kaempferol in Penthorum Chinense Pursh by HPLC
Chinese Journal of Information on Traditional Chinese Medicine 2016;23(3):77-79
Objective To establish an HPLC method for the simultaneous content determination of quercetin and kaempferol in Penthorum Chinense Pursh. Methods The HPLC analysis was carried out on Kromasil C18 (250 mm× 4.0 mm, 5.0 μm) with a mixture of methanol-0.1% phosphate (50:50) as the mobile phase. The determination wavelength was set at 360 nm with the flow rate of 1.0 mL/min. The column temperature was set at room temperature. Results The quercetin and kaempferol showed good linearity in the range of 0.105 6–2.112 0 μg and 0.023 6–0.472 μg respectively. The average recovery rates of quercetin and kaempferol were 98.46% (RSD=2.29%) and 98.17%(RSD=1.99%) respectively. Conclusion The method is accurate and sensitive, which can be used for the content determination of quercetin and kaempferol in Penthorum Chinense Pursh.
3.Determination of Cinobufagin and Resibufogenin in Qianglijiuxin Dripping Pills by HPLC
Xingping LUO ; Lanxia WANG ; Fen WANG ; Bochong ZHANG ; Ruichao LIN ;
Chinese Traditional Patent Medicine 1992;0(07):-
Objective: To determine the contents of cinobufagin and resibufogenin in Qianglijiuxin Dripping Pills. Methods: The HPLC method was used at Kromasil C 18 column of 250mm?4.6mm, 5?m, 0.5% Dipotassium Hydrogen Phosphate Acetonitrile (50∶50) as the mobile phase, 1.0mL/min as the flow rate at detection wavelength of 296nm. Results: For cinobufagin, the linear range was 0.67?g~4.7?g and the average recovery rate was 97.78% with RSD=1.2%. For resibufogenin, the linear range was 0.33?g~2.3?g and the average recovery was rate 99.61% with RSD=2.0%.Conclusion: The method can be applied to content determination of cinobafagin and Resibufogenin in Qianglijiuxin Dripping pills.
4.Research advances in the clinical features of liver disease during pregnancy
Xingping DUAN ; Yan WANG ; Changyou HUANG ; Huixian ZHENG ; Yi LUO ; Zhengwen QIN ; Yu REN
Journal of Clinical Hepatology 2020;36(6):1407-1411
Severe liver disease during pregnancy is uncommon in clinical practice. The most common cause of liver disease during pregnancy is liver dysfunction, with an overall prevalence rate of approximately 3%. Liver disease during pregnancy is classified into the liver diseases directly caused by pregnancy and those co-existing with pregnancy, i.e., pre-existing liver disease or occasional liver disease during pregnancy. A differential diagnosis of pre-existing and co-existing liver diseases may help to improve maternal and fetal outcome. During clinical diagnosis and selection of treatment and intervention measures, priority should be given to the potential impact on mother and fetus. This article introduces the latest research advances in the general information, pathogenesis, treatment, and pregnancy outcome of major liver diseases during pregnancy and elaborates on the risk of pregnancy and related coping measures for patients with pre-existing liver disease, so as to guide clinical diagnosis and treatment and patient management.
5.Clinical utility of tuberculosis protein chip in diagnosis of tuberculosis
Yougen WU ; Xingping YANG ; Jun WANG ; Hongbing LIU ; Juhua LUO ; Bi YU ; Shuyuan XIAO ; Li ZHANG ; Chaojin LI
Chinese Journal of Infection and Chemotherapy 2014;(3):196-198
Objective To explore the potential value of tuberculosis protein chip for clinical diagnosis of tuberculosis.Methods The antibody level of tuberculosis protein ESAT-6,CFP10,16 KD,38 KD and LAM was determined in 4 093 patients,inclu-ding 441 tuberculosis and 3 652 non-tuberculosis cases by protein chip.Results The tuberculosis antibody was positive in 297 of the 441 tuberculosis cases and 647 of the 3 652 non-tuberculosis cases.Tuberculosis protein chip provided a sensitivity of 67.35% and specificity of 82.28% in the diagnosis of tuberculosis.Conclusions Tuberculosis protein chip test is a quick,easy and effective method for identifying potential tuberculosis patients with good specificity.