Lysozyme, pepsin, ovalbumin, hemoglobin andγ-globumin were chosen as templates to investigate the imprinting capability of amphoteric polyacrylamide cryogels. Prepolymerizing solutions contained acrylic acid and allyl amine, as well as acrylamide and N,N'-methylenebisacrylamide as the functional monomers. As a result there were both acidic and basic functional groups in the polymers, facilitating effective interactions with likewise amphoteric proteins. The proteins differ greatly and cover wide scopes of molecular weights and isoelectric points. Regardless of the values of the molecular weights and isoelectric points, all the templates gave higher retentions on the MIP tubes than on the NIP tube. The MIP of lysozyme indicated the highest imprinting factor of 7. 0, and that of γ-globumin showed the lowest, 2. 0. The values of other proteins were intervenient. Conclusively the amphoteric polyacrylamide cryogels were suitable imprinting materials for various proteins, and could potentially be used for protein recognition, purification, and depletion.

Eggwhitesolutionswereusedas“pendingtemplates”topreparemolecularimprintedpolymers. The obtained polymers acted as stationary phases, exhibiting abilities to deplete abundant proteins in the egg white. The abilities of protein depletion of the imprinted polymers could be adjusted by using different concentrations of egg white in preparing the polymers. After chromatographically processed in a home-made syringe system, abundant proteins such as ovalbumin, lysozyme, and ovotransferrin were removed from egg white sample solutions. Along with the disappearance of the mass spectrum peaks of these proteins, the signals of other components became more obvious. Meanwhile according to reference data and molecular weights, they were reasonably ascribed to be ovalbumin-related proteins, ovotransferrin-related proteins, ovomucoids or flavoproteins respectively. Conclusively, pending imprinting method has the power to deplete abundant proteins, and to retain and enrich posttranslationally modified species at the same time.

Objective To investigate the clinical indicators which can predict esophageal varices in patients with primary biliary cirrhosis (PBC).Methods A total of 351 patients with PBC from the First Affiliated Hospital of Xinjiang Medical University from January 2013 to December 2016 were retrospectively analyzed, including 173 patients with esophageal varices and 178 patients without varicose veins.The alanine aminotransferase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), γ-glutamyl transpeptadase (γ-GT), total bilirubin (TBil), albumin (Alb), prothrombin time (PT), platelet (PLT), AST to ALT ratio (AAR), fibrosis index based on the 4 fator (FIB-4), AST to PLT ratio index (APRI) and Mayo scores were compared between two groups.Group t test or rank sum test was used to compare the two groups.Relation between the indicators mentioned above and esophageal varices were tested by univariate analysis.Multivariate unconditional Logistic regression was used to screen these indicators to independently predict esophageal varices in PBC patients.Results Age, PT, TBil, AAR, FIB-4, APRI and Mayo scores of PBC patients with esophageal varices were all higher than those of patients without esophageal varices ([60.3±10.6] years old vs [51.9±10.9] years old, [13.31±3.12] s vs [11.17±2.42] s, 28.06 [18.05, 60.06] mmol/L vs 15.39 [10.64, 33.63] μmol/L, 1.69±0.91 vs 1.23±0.95, 6.18 [4.05,9.16] vs 1.80 [1.10,2.74], 1.95 [1.12,3.08] vs 0.69 [0.38,1.57], 6.45±1.52 vs4.62±1.53, respectively).Whereas ALT, γ-GT, Alb and PLT levels were all lower than those without varicose veins (36.60 [19.88, 74.28] U/L vs 59.32 [23.58, 132.70] U/L, 71.00 [38.36, 165.38] U/L vs 125.00 [37.50, 336.21] U/L, [29.78±6.33] g/L vs [39.51±25.16] g/L, [103.43±52.84]×109/L vs [234.44±90.40]×109/L, respectively).The differences were all statistically significant (t=-7.25, t=-7.18, Z=-5.823, t=-4.60, Z=-8.427, Z=-12.661, t=-11.25, Z=-3.218, Z=2.987, t=4.94, t=16.63, respectively;all P<0.01).Multivariate unconditional logistic regression analysis showed that PLT<149×109/L (OR=0.966, 95% CI: 0.957-0.974), PT>11.95 s (OR=0.705, 95%CI: 0.569-0.874), TBil>17.19 μmol/L (OR=0.99, 95%CI: 0.982-0.999), FIB-4>3.02 (OR=0.868, 95% CI: 0.807-0.932) and Mayo score>4.88 (OR=6.053, 95%CI: 2.388-15.342) were independent clinical indicators for the prediction of esophageal varicose veins.Conclusions PLT, PT, TBil, FIB-4, and Mayo scores can be used as predictors of esophageal varices in patients with PBC.

Primary biliary cirrhosis as autoimmune liver disease, due to conventional screening in recent years, the popularity of anti-mitochondrial antibodies to the number of patients with early primary bil-iary cirrhosis showed an upward trend. The development of esophageal varices is an important indicator of poor prognosis of primary biliary cirrhosis. The current assessment of esophageal variceal gold standard is endoscopy, but it is invasive examination, such as non-invasive examination to assess whether patients with esophageal varices, the selective endoscopy of the patient, can significantly reduce the patient's pain and the economic burden. In recent years, clinical studies have been carried out or carried out with non-invasive de-tection of patients with primary biliary cirrhosis complicated by esophageal varices.

Objective To study the characteristic findings on magnetic resonance imaging (MRI) in children with cerebral palsy (CP) induced by chronic kernicterus.Methods The MR imagings in 13 CP cases induced by chronic kernicterus were retrospectively studied, which were compared with 6 healthy and age-matched controls. 13 cases were finally diagnosed as dyskinetic cerebral palsy (athetoid subtype).Results In 13 cases, all showed symmetric high signals in the the globus pallidus on T2-weighted imaging. There were not apparent abnormality on T1-weighted imaging.Conclusion The bilateral high intensity signals in the globus pallidus on T2-weighted imaging are the characteristic findings on MRI in CP children induced by chronic kernicterus. Paying attention to the globus pallidus on MRI may be useful in determining the type of cerebral palsy.

Objective: To investigate knowledge, attitude and behavior related to tuberculosis prevention among primary and elementary school students in Heilongjiang province, and to provide evidence for school-based tuberculosis prevention and control. Methods: A cross-sectional survey method was used to conduct a questionnaire survey among 3 033 middle and elementary school students in 10 counties (districts) of Heilongjiang province, including knowledge, attitudes and behaviors related to tuberculosis prevention and treatment. Results: In the survey, 61.5% of the students understand the core information of tuberculosis. The core knowledge awareness rate was higher in primary school students (64.8%) than in middle school students(58.1%)(χ2=85.41，P<0.05). Primary and middle school students indicated that the attitude-taking rate of concerns about the tuberculosis patients was 47.5%. Primary school students had more general attitude than middle school students towards willingness to participate in publicity activities compared with primary school students(χ2=39.26，P<0.05). About 85% of students reported personal hygiene habits. The results of multiple linear regression analysis showed that tuberculosis-related knowledge was mainly associated with factors including urban and rural, parental education, health education courses in school(P<0.05). Conclusion There is a gap in tuberculosis-related knowledge among primary and middle school students between Heilongjiang province and national level. There’s a need for the improvement of tuberculosis control programs to inform a better understanding and social dissemination of TB information.

Objective A retrospective analysis was carried out for the classification and character-istics of fever of unknown origin, and its trend in recent years. Methods A total of 1192 patients with fe-ver of unknown origin, who were admitted to the First Affiliated Hospital of Xinjiang Medical University from January 2010 to December 2016, were enrolled in this study. Descriptive analysis of the proportion, charac-teristics, and trend different diseases in the fever of unknown origin was performed. Results A total of 1080 cases was diagnosed in 1192 patients, with a diagnosis rate of 90. 6％. The final diagnoses included infections, inflammatory diseases, malignancies and miscellaneous conditions in 72. 9％, 9. 1％,5. 1％ and 3. 4％ of subjects, whereas 9. 4％ of cases remained undiagnosed. The results of variance analysis showed that the white blood cells and hemoglobin of infectious diseases were higher than those of connective tissue disease and malignancy were respectively [(11. 12 ± 2. 58) × 109/L vs (8. 82 ± 1. 12) × 109/L vs (9. 67 ±2.76) ×109/L, (116.81 ±20.77)g/L vs (103.25 ±22.84)g/L vs (102.30 ±25.14)g/L], While period of hospitalization and erythrocyte sedimentation rate were lower than that of connective tissue disease and malignancy were respectively [ ( 12. 15 ± 7. 91 ) days vs ( 19. 94 ± 10. 17 ) days vs ( 18. 85 ± 14. 37 ) days, (48.12 ±20.93)mm/h vs (58.13 ±20.24)mm/h vs (56.9 ±20.52)mm/h], and the age of ma-lignant was higher than that of infectious disease and connective tissue disease were [(56. 38 ± 17. 57)years vs (45. 99 ± 16. 79)years vs (41. 09 ± 16. 79)years], the difference was statistically significant (P<0. 05).The rank sum test showed that eosinophils, lymphocytes and interleukin-6 were not equal ( P <0. 05 ) . Conclusions Infectious diseases in fever of unknown origin ( FUO) are still the main cause. whereas blood cells, eosinophils, lymphocytes, hemoglobin,erythrocyte sedimentation rate, interleukin 6, age and hospi-talization time were predictive of disease diagnosis. In recent years, there was no obvious trend of all kinds of diseases.

Objective To describe the Xinjiang region of tuberculous meningitis (TBM) (age ＞ 18 years of age) in patients with the clinical features,analysis of factors influencing the prognosis.Methods A retrospective of cases included in the data collection,describe the clinical characteristics,using the single factor and multiple factors Logistic regression analysis of factors influencing the prognosis.Results A total of 200 cases of TBM patients had the following characteristics:120 cases of male,female 80 cases;age 18 -75 (37.35 ±+ 14.24) years old;the course of the disease 3-270 (36.86 +43.86)days;and 179 cases of survival,21 cases of death,with a mortality of l0.5％.Single factor analysis showed that hydrocephalus,encephaledema,clinical staging,erythrocyte sedimentation rate,cerebro-spinal fluid (CSF)-protein had statistically significant difference between survival group and death group,respectively (P ＜ 0.05).The multi-factor Logistic regression analysis showed that encephaledema and erythrocyte sedimentation rate were independent factors to affect the prognosis of TBM (P ＜ 0.05).Conclusions Encephaledema and erythrocyte sedimentation rate are independent risk factors of poor prognosis in patients with TBM,early identify predictors,formulate corresponding clinical countermeasures,and can improve the prognosis of patients with tuberculous meningitis.