Objective To find a new operation based on the themy of NOTES,through the way of mouth floor-pretracheal space-retrosternal space,instead of through esophagus or the trachea.Methods We anesthetized six mongrel dogs with endotracheal intubation,then cut the tissue between the incisors and the glossodesmus.The retrosternal space was separated slowly by the balloon expander,and cut the right mediastinal fascia,and went through thorax,then cut a small piece of lung tissue,which would be used to biopsied.Results All of the 6 operations were successfully completed,without any arrhythmia,serious bleeding,airway obstruction and death.Conclusion It doesn't need the soft endoscope in the new operation,which will have bigger operating space and to be safer,just with pin type thoracoscope and related apparatus.

Objective: To prepare mesalazine thermosensitive liquid suppositories and study the in vitro properties. Methods:Poloxamer 407 (P407) and poloxamer 188 (P188) were used as the temperature-sensitive materials, and mesalazine thermosensitive liquid suppositories were prepared by a cold method. The optimal ratio of P407 and P188 was screened using gel temperature as the in-dex. The gel strength and bioadhesive force of mesalazine thermosensitive liquid suppositories were studied, and the in vitro gel erosion and drug release were investigated as well. Results:The optimal ratio of P407 and P188 was 20%: 2. 5%, and the gel temperature was (36.9 ±0.2)℃, the gel strength was (115.1 ±3.2)s and the bioadhesive force was (130.7 ±5.8) ×102 dyne·cm-2. The drug release and gel erosion in vitro were both fitted first-order kinetics, and the two had promising linear relationship, suggesting good sustained-release property based on gel erosion mechanism of mesalazine liquid suppositories. Conclusion:The preparation of mesala-zine liquid suppositories is simple, and the gelation, bioadhesion and sustained-release of the suppositories are promising. Mesalazine liquid suppositories are valuable to be studied further.

ObjectiveTo explore the clinical value of 256-slice CT and coronary angiography in the diagnosis of coronary artery disease.Methods46 patients with coronary heart disease,who underwent coronary angiography and 256-slice spiral CT were selected.Compare two methods for the detection of coronary artery disease.Results( 1 ) All 256-slice CT and coronary angiography achieved a satisfactory image,in the diagnosis of coronary artery stenosis and occlusion,the two methods had no statistically significant difference ( P ＞ 0.05 ).(2) according to standard coronary angiography,256-slice spiral CT for the diagnosis of coronary artery stenosis sensitivity was 93.0％ (132/142),specificity was 91.6％ (142/155),positive predictive rate was 91.0％ (132/145),negative predictive rate was 93.4％ (142/152).Conclusion256-slice spiral CT has a high time resolution,spatial resolution,the display of the coronary artery disease has high sensitivity,specificity,positive predictive value and negative predictive value.

Objective To examine the effect of family environment and family function on suicide behavior in schizophrenic patients.Methods 53 schizophrenic patients with suicidal behavior in the last month were selected for the schizophrenic suicide group.53 schizophrenic patients and 53 normal controls matched with the schizophrenic suicide group were selected for the schizophrenic no suicide group and normal control group.All of them completed family environment scale and family assessment device.Results The cohesion factor,the expressiveness factor,the active-recreational orientation factor and the control factor of family environment scale in the schizophrenic suicide group were significant lower than those in the schizophrenic without suicide group(separately 5.70 ± 1.29 vs 6.62± 1.23,4.77 ± 1.49 vs 5.96 ± 1.11,3.94 ± 1.63 vs 5.68 ± 1.98,2.68 ± 1.68 vs 4.40 ± 1.81,P＜ 0.05),but the conflict factor in the schizophrenic suicide group was higher than in the schizophrenic without suicide group(3.55 ± 1.60 vs 2.81 ± 1.52,P ＜ 0.05).The affective responsiveness factor,the affective involvement factor and the behavior control factor of family assessment device in the schizophrenic suicide group were significant lower than those in the schizophrenic without suicide group(separately 16.05 ± 2.57 vs 14.53 ± 3.38,19.13 ± 4.09 vs 16.58 ± 2.82,23.35 ± 3.52 vs 21.60 ± 3.25,P ＜ 0.05).Multiple liner regression analyses showed that the expressiveness factor,the active-recreational orientation factor,the control factor,the affective involvement factor and the behavior control factor were important factors for schizophrenic suicide(F=21.583,P=0.000,adjusted R 2 =0.519).Conclusion Attempted suicide patients with schizophrenia have some deficiencies at family function and family environment.

Objective:To prepare thermosensitive liquid suppositories of mesalazine( MSZ) HP-β-CD complex and study the in vitro properties. Methods:Poloxamer 407 (P407) and poloxamer 188 (P188) were used as the thermosensitive polymers to load MSZ HP-β-CD complex to prepare the thermosensitive liquid suppositories. The viscosity, gel strength, bioadhesion,in vitro gel erosion and drug release of MSZ HP-β-CD complex were compared with those of MSZ liquid suppositories. Results: Compared with that of MSZ liquid suppositories, the gel temperature of MSZ HP-β-CD complex liquid suppositories was stable, while the viscosity, gel strength and bioadhesion of MSZ HP-β-CD complex liquid suppositories were increased significantly. The in vitro gel erosion was not affected by MSZ HP-β-CD complex, while the drug release was notably enhanced. The drug release and gel erosion showed a good linear relation-ship. Conclusion:MSZ HP-β-CD complex liquid suppositories show good gel temperature, viscosity, gel erosion and bioadhesion, and the in vitro release rate is faster than that of MSZ liquid suppositories.

Objective:Study on characteristics of two synthesizd peptides based on CSFV E2 protein. Methods:B cell epitopes of CSFV E2 antigen were predicted using accessibility and flexibility schemes, associated with antigenicity , secondary structure and multiple sites prediction. Two antigen peptides (Pep1 and Pep2) have been designed and synthesized and their reactivety were detected with 8 McAbs and antiserum against mE2 protein, then the peptides were conjugated with BSA and immunized rabbits respectively. Results:Both Pep1 and Pep2 could react with antiserum and McAb A11, Pep2 could interact with McAbD5 and McAbD8. Only Pep1 BSA conjugate can stimulate high level and specific antibodies.Conclusion: The peptide1 has good antigenicity.

BACKGROUND: Inhibitory coating can prevent nanoparticle oxidation, grain growth, corrosion and agglomeration, and endow nanoparticle with special properties. ABJECTIVE: To prepare SiO2/Ni core-shell type nanoparticles and assess their magnetic properties. DESIGN, TIME AND SETTING: The observation experiment was performed between November 2005 and March 2006 at Nanometer Compound Material Research Laboratory of Dalian University of Technology, Dalian, Liaoning Province, China. MATERIALS: Nanometer nickel powder prepared by DC arc plasma jet method, Na2SiO3 produced by Bazhou Chemical Industry Branch Factory of Tianjin Quartz Clock Factory (China).METHODS: SiO2/Ni core-shell type nanoparticles were synthesized by coating a layer of SiO2 on the surface of manometer nickel powder via liquid deposition method using Na2SiO3 as the main source material. MAIN OUTCOME MEASURES: Their microstructures and material properties were investigated by X-ray diffraction, Fourier transform infrared spectrometer, transmission electron microscopy, thermo-gravimetric analysis, differential scanning calorimetry and vibrating sample magnetometer. RESULTS: The experimental results showed that SiO2 shell was in an amorphous state around Ni cores and it avoided agglomeration of the Ni nanoparticles. The oxidation temperature of nanometer nickel powder coated by SiO2 elevated from 287 ℃ to 385 ℃. The analysis result of magnetic properties indicated that the hysteresis loop of Ni had an excursion for the existence of anti-ferromagnetic NiO, the silica coating reduced the saturation magnetization and improved the coercivity. CONCLUSION: Preparation of SiO2/Ni core-shell type nanopartieles was successful; silica coating improved the oxidation resistance of nanometer nickel powder, endowed nanometer nickel powder better ferromagnetism and improved the coercivity.

Objective:To study the effect of mesalazine hydroxyl-propyl-β-cyclodextrin inclusion compound ( MSZ-HP-β-CD) liq-uid suppositories on ulcerative colitis ( UC) in rats. Methods:Dextran sulfate sodium was used to induce UC in rats. The marketed MSZ suppositories were applied as the positive control, and the changes in morphology and histopathology of the UC rats were observed after the treatment with MSZ-HP-β-CD liquid suppositories. Results:The results of morphology study showed that CMDI was decreased in the two MSZ preparation groups when compared with that in the model group (P<0. 01), and that in MSZ-HP-β-CD liquid supposi-tories group was the lowest (P<0. 05). The results of histopathology study showed that with the treatment of the marketed MSZ sup-positories and MSZ-HP-β-CD liquid suppositories, the ulcer and damage were improved, and the improvement in the MSZ-HP-β-CD liquid suppositories group was more significant. Conclusion: MSZ-HP-β-CD liquid suppositories exhibit better effect on UC in rats when compared with the marketed MSZ suppositories, which is worthy of further studies.

Objective Toanalyzetheclinicalcharacteristicsandtreatmentoutcomesofspinalfilum terminalevascularmalformation.Methods Theclinicaldataof6patientswithfilumterminalevascular malformation diagnosed and treated from January 2008 to December. 2013 were analyzed retrospectively. The definition of filum terminale vascular malformation is anterior/posterior spinal artery feeding arteriovenous fistula or arteriovenous malformation and located below conus medullaris,and does not complicate with spinal vascular lesions in the other part. The Aminoff & Logue score and MRI of spinal cord function were performedatoneyearaftermicroneurosurgeryand/orendovascularembolization.Results Allpatients were males. Their clinical presentations were the weakness of both lower extremities and sphincter disturbance. The mean course of disease was 17. 1 ± 5. 2 months. The pathological type of the 6 patients were all arteriovenous fistulas. The feeding arteries included lumbar artery,internal iliac artery,and median sacral artery. Two of the 6 patients underwent Onyx glue embolization,3 were treated with microneurosurgery,and 1 was treated with embolization in combination with microneurosurgery. They were all achieved anatomic cure. The Aminoff & Logue scores were improved after 1 year (3. 8 ± 1. 9 scores before procedure,2. 8 ± 2. 0 scores after procedure),there was no significant difference (P >0. 05). The myodynamia scores were improved in 3 patients,2 did not change,1 got worse. The urinary and bowel functions were improved in 2 patients,and4didnotchange.Conclusion Filumterminalevascularmalformationisararevascular malformation of spinal cord. Both embolization and surgical treatment can achieve anatomic cure. Although the spinal cord function can be only partially restored,but continuous deterioration can be prevented.

This study was aimed to observe the effect of Bai-Zhu Fu-Ling (BZFL) Decoction in different proportion-ing on VIP and VIPR1 in Crohn's disease (CD) rats with spleen deficiency syndrome, in order to further explore the immunologic mechanism of BZFL Decoction on CD. The CD rat model with spleen deficiency syndrome was estab-lished using exhaustion and hunger. The model rats were treated by BZFL Decoction with different proportioning, and immunohistochemistry (IHC) was used to detect the expression of VIP and its receptor in colon tissues. The results showed that comparing to the blank control group, the level of VIP and its receptor of the model group significantly increased (P< 0.05). Comparing to the model group, the level of VIP and its receptor in BZFL Decoction B5 group (Rhizoma A tractylodis Macrocephalae:Poria = 12:15), B6 group (Rhizoma A tractylodis Macrocephalae:Poria = 15:12) and B7 group (Rhizoma A tractylodis Macrocephalae:Poria = 18:9) was significantly decreased (P< 0.05). It was con-cluded that the effect of BZFL Decoction of B5 group, B6 group and B7 group was better than other groups in VIP and its receptor which can regulate the VIP and its receptor, inhibit the releasing of inflammatory factors and reduce intestinal inflammation injury.