The chemical stability of aqueous solution of ferulic acid was studied by the constant temperature accelerated test according to the princiPle of chemical dynamics. The ferulic acid in sample was determined by HPLC. The result showed that the decomposition of ferulic acid in aqueous solution accords with the primary reaction. At room temperature (25℃),the time of decomposition of 10℃ ferulic acid in aqueous solution (the expiry date of drug) was calculated robe 19. 36mo and 17.41 too-20. 69mo,calculated by classical and simple calculation methods, respectively.

Objective To investigate the treatment of proximal femoral nail antirotation (PFNA) in pertrochanteric hip fractures with unstable lateral-wall.Methods Thirty-two cases were included from the April 2009 to May 2011.Recorded operation period,blood loss volume,and complications and evaluated the effect of the methods.Arrange referral at 6 weeks,3 months,6 months,12 months respectively after operation,to check limb function and conventional camera hips,lateral X-ray film.Postoperative functional evaluation using the Harris hip score.Measure the last follow-up neck shaft angle and compare with postoperative's.Results The average operation period was (60-190) min,average was (80 ± 5) min ; blood loss volume was (90-250)ml,average was (120 ± 9) ml ; Average hospital periods was (15 ± 7) days; weight loading time was (6.3 ± 1.2)weeks after surgery and average time of union of fracture was 3.6 months.All patients obtained union of fracture without complications such as varus,internal fixation cutting out.Conclusion PFNA was proved to be an effective way for treating unstable lateral-wall of pertrochanteric hip fractures due to its advantages of stable fixation,minor trauma,less complications.

Objective To analyse postoperative co mp lications of ultrasonic ablation and transluminal therapy in patients of deep v enous thrombosis(DVT) in the lower extremities. Meth ods In this study, 205 DVT patients with a history fro m 6h to 90d were treated by a combination of ultersonic ablation、Fogarty ca theter thrombectomy、balloon dilatation and stent placement. Results Major complications developed in 37 cases (18.05%) including vein perforation, thrombosis, bleeding and anaphylaxis to contrast medium, except for minor complications such as lymph exudation. The re was no mortality in this series. Conclusions This procedure is safe, miniinvasive and effective therap y for DVT patients.

Objective To compare the clinical outcomes between proximal femoral nail antirotation (PFNA) combined with risedronate sodium and isolated PFNA for the treatment of intertrochanteric fractures in elderly patients.Methods Clinical data of 62 elderly patients with intertrochanteric fracture from January 2011 to April 2013 were prospectively studied.Patients were randomly divided into two treatment groups:risedronate sodium group (the combined PFNA and risedronate) and control group (isolated PFNA).According to AO classification,32 patients in risedronate sodium group (15 males and 17 females,with an average age of 78 years) were divided into type A1 (n=10),type A2 (n=18) and type A3 (n=4).30 patients in control group (13 males and 17 females,with an average age of 77.5 years) were divided into type A1 (n=9),type A2 (n=16) and type A3 (n=5).Bone mineral density (BMD) was measured by dual energy X-ray absorptiometry before and 1 year after operation.The hip joint function was assessed by Harris hip score.Complications and subsequent fragility fractures were evaluated postoperatively.Results 60 patients (30 patients in risedronate sodium group and 30 patients in control group) were followed up for at least one year.All fractures were healed at 6 months after surgery.No significant difference was found between the two groups in BMD in contra-lateral hip before treatment [(-2.58±0.41)kg/m2 vs.(-2.56±0.36)kg/m2,P＞0.05].BMD in contra-lateral hip had significant difference between the risedronate sodium and control groups one year after surgery [(-0.66±0.37)kg/m2 vs.(-1.13 ±0.28)kg/m2,P=0.000].There was no significant difference between the two groups in Harris hip score (P=0.238).During the follow-up,no patient in risedronate group suffered from subsequent fragility fracture after surgery,while lumbar compression fracture occurred in 1 case,distal radius fracture occurred in 1 case,the contralateral hip fractures occurred in 2 cases,postoperatively.Conclusions PFNA treatment combined with risedronate sodium can effectively improve BMD and reduce the risk for refracture,and has a good effect on intertrochanteric fracture in elderly patients.

ObjectiveTo investigate the causes of groin lymphatic fistula after transluminal ultrason ic angioplasty (TUA) in the treatment of deep vein thrombosis(DVT) of lower extremity through groin incision. Methods A retrospective anal ysis of the causes of groin lymphatic fistula(GLF) in 72 patients with DVT after treatment of ultrasonic ablation was made. ResultsTUA and thr ombectomy using Forgaty balloon catheter for DVT of lower extremity achieved successful recanalization in all the 72 cases (100%). Of the 72 cases,51 had i liac vein stenosis,the venous stenosis disappeared after balloon dilation;amo ng the 51 cases ,6 cases received stent placement. GLF occurred postoperati vely in 60 cases. No death occurred in this series. ConclusionsTUA is an effective method in the treatment of DVT of lower extremity,but t he incidence of postoperative GLF is high.The causes of GLF may be related to the injury of lymphatic vessels, increase of quantity of lymph flow induced by increased venous pressure, infection, surgical technique factors and postop erative medicine therapy etc.

Objective To investigate the effect of periulcerous suturing on the healing of skin ulceration in patients with chronic deep venous thrombosis. Methods Thirty-two patients were divided into two groups: control group treated with drugs and compression therapy; suturing group treated with drugs, compression therapy and skin suturing around the ulceration. The clinical data of these cases were retrospectively studied. Result In the suturing group, perforator veins (1.0±0.8) decreased, oxygen saturation of venous blood (39±7) mm Hg and transeutaneous oxygen monitoring (tcPO_2) (71±12)mm Hg were elevated, the number of mitochondrion increased and white cell infiltration decreased, the figure of rough surfaced endoplasmic reticulum recovered, ulcer healing (12±6) d quickened and recrudescence (12.5%) lessened after treatment. All differences were statistically significant when compared with those in the control group (P<0.05). Conclusion Suturing around the ulceration can lighten blood stagnation and inflammatory cell infiltration, improve dystrophy and oxygen supply of the tissue, shorten the period of ulcer healing. It is a simple, good therapeutic and economical method to cure ulcer and can decrease the recurrent rate with physiotherapy.

Objective:To summarize experience of 8 lower limb deep venous thrombosis(DVT) patients who used OptEase recyclable vesa cava filter(VCF) during in operations.Methods:Through collecting the clinical data of 8 patients who used OptEase recyclable vena cava filter(VCF) ,analyzing the method of recycling and releted complications.Results:No pulmonary embolism happened during and after operations,when recycle VCF implanted,vena cava graphy shows:inperfection thrombous near the filter in 1 case and vena iliaca thromb of uninjure side in 1 case too,reclaiming successfully in 5 cases,3 cases turn to permanent placement.FolIowing-up observation for 3-8 months,recurrence of limbs vena thrombosis never happened.Conclusion:The recyclable vena cava lilter could prevent plamonary embolism effectively in lower limb deep venous thrombosis patients.

Objective To assess temporary filter in the inferior vena cava (IVC) for trapping thrombus and preventing pulmonary embolism in patients with deep vein thrombosis. Methods In 58 cases of deep vein thrombus in single lower extremity, Antheor temporary filter (ATF) was introduced into IVC prior to anticoagulation and/or vascular intraluminal procedures. Results All IVC filters were successfully introduced. No symptomatic pulmonary embolism was documented. ATF was removed after a mean of 12.0?2.0 days. Thrombus was trapped in 46 cases (79.3%). Thrombus more than 1 cm in size was trapped in 2 cases. Conclusion Temporary filter was safe and effective in prevention of pulmonary embolism.

Objective To establish a quantitative detection method for Mycobacterium tuberculosis by immunomagnetic capture combined with PCR-ELISA detection system with double internal standards(IMC-PCR-ELISA) .Methods The immunomagnetic (Dynabeads? ) which could specifically capture Mycobacterium tuberculosis were prepared .According to Mtp40 and IS6110 gene sequence of Mycobacterium Tuberculosis ,2 pairs of primers(upstream primer was modified with Biotin at 5′end) ,2 same-length mutant fragments with PCR amplified fragments ,and 3 capture probes(modified with digoxigenin at 3′end) were designed .Myco-bacterium tuberculosis were captured by immunomagnetic ,then detected by PCR-ELISA with double internal standards .Results The IMC-PCR-ELISA method could yield quantitative results in about 4 h with a detection limit at 5 × 103 copies/mL .There was a fine linear relationship between the copies of Mtp40(IS6110)in fact and in the calculation through formula when the concentrations of low internal standards were 30-70 copies/mL and the concentrations of high internal standards were 8 000-12 000 copies/mL (r2 =0 .998) .No nonspecific amplification was observed .Conclusion A rapid and quantitative method for the detection of Myco-bacterium tuberculosis was established successfully .The IMC-PCR-ELISA method was rapid ,sensitive ,secific and quantitative .

Objective To study methods of salvage therapy for acute lower extremity(ALEI) ischemia.(Methods) A restrospectively analyzsis was made on the clinical data of 96 patients with consecutive 106 ALEI limbs.In which Fogarty catheter embolectomy was used as the initial treatment for ALEI in a regular(operating) room or in an intervention therapy room under DSA monitoring,and graft bypass after embolectomy was performed on 8 cases(12 limbs).Results In 70 cases(75 limbs) successful embolectomy of the(iliac),femoral,popliteal and tibial artery was achieved.In 11 cases(12 limbs) embolectomy at distal to the popliteal artery was unsuccessful.Eight cases(12 limbs) with obstruction proximal to the femoral artery were treated by axillofemoral bypass in 4 cases,and femorofemoral bypass in 4 cases.One case of thoracoabdominal aortic dissection aneurysm and lower extremity ischemia had fenestration of the abdominal aoric dissection.Proximal embolectomy and distal amputation was performed in 6 cases.Fimally,76(79.2%) cases had(salvaged) limbs,11(11.4%) patients had amputated limbs,and 9(9.4%) patients died.Seventy-six(patients)(85 limbs) were followed up for 1 to 38 months,7 cases(7 limbs) were reoperated on because of recurrent embolism.Conclusions Embolectomy is the effective treatment method for ALEI.Embolectomy(under) DSA monitoring can improve therapeutic results.The prognosis lies on the duration and extent of(ischemia),and management of complications.