From August 21 to December 13, 2018, a tetramine poisoning incident in Wenzhou, Zhejiang Province was investigated, and the clinical diagnosis and treatment of tetramine poisoning was analyzed. There were 6 cases of poisoning caused by artificial tetramine poisoning. The diagnosis was delayed, coma and convulsions were severe manifestations continuous renal replacement therapy (CRRT) was effective in the treatment of severe cases, and all 6 cases were cured. The possibility of poisoning should be considered for unexplained coma and/or convulsions. Although tetramine is banned, it still needs to be highly vigilant and avoids the recurrence of delayed diagnosis and treatment.

From August 21 to December 13, 2018, a tetramine poisoning incident in Wenzhou, Zhejiang Province was investigated, and the clinical diagnosis and treatment of tetramine poisoning was analyzed. There were 6 cases of poisoning caused by artificial tetramine poisoning. The diagnosis was delayed, coma and convulsions were severe manifestations continuous renal replacement therapy (CRRT) was effective in the treatment of severe cases, and all 6 cases were cured. The possibility of poisoning should be considered for unexplained coma and/or convulsions. Although tetramine is banned, it still needs to be highly vigilant and avoids the recurrence of delayed diagnosis and treatment.

Objective To investigate the protective effect of P-glycoprotein up-regulated by ulinastatin (UTI) on HK-2 cells during paraquat (PQ)-induced injury and its underlying mechanisms.Methods The research was divided into two parts.The first part of the research was divided into normal control group,PQ group,UTI+PQ group,UTI control group.The second part of the research was divided into negative virus group (including control group,PQ group,PQU+TI group,UTI group) and P-gp siRNA group (including control group,PQ group,PQU+TI group,UTI group).Negative virus group:the cells were transfected into the blank virus;siRNA P-gp group:the cells were transfected with P-gp siRNA virus.HK-2 cells were routinely cultured.After 800 μmol/L PQ treatment,the changes of P-gp protein levels in the HK-2 cells were determined by Western-blot (WB).Then,transfected lentivirus bringing P-gp silent gene,the cell viability was determined by CCK-8 assay,the expression of P-gp in the cells after transfection was detected by WB and the concentration of PQ in HK-2 cells were measured by high performance liquid chromatography (HPLC).Results Compared with the normal control group,the P-gp expression of PQ group had no significantly changes (P＞0.05).Compared with the PQ group,the P-gp expression of UTI +PQ group significantly increased (P＞0.05).Compared with the corresponding control siRNA group,the P-gp siRNA group had no significantly changes in cell viability (P＞ 0.05).and significantly decreased in P-gp expression.Compared with the corresponding control siRNA group,the P-gp siRNA group had no significantly changes in PQ concentration in HK-2 cell (P＞0.05),but compared with P-gp siRNA PQ group,the PQ concentration of P-gp siRNA PQ+UTI group significantly decrease(P＜0.05).Conclusion UTI significantly reduced the accumulation of PQ in HK-2 cells and increased the viability of HK-2 cells in vitro may be not by increased P-gp activity.UTI could significantly reduce HK-2 cell injury induced by PQ in vitro and improve the survival rate of HK-2 cells.It may not be related to the up regulation of P-gp expression.

Objective To investigate the protective effect of P-glycoprotein up-regulated by ulinastatin (UTI) on HK-2 cells during paraquat (PQ)-induced injury and its underlying mechanisms.Methods The research was divided into two parts.The first part of the research was divided into normal control group,PQ group,UTI+PQ group,UTI control group.The second part of the research was divided into negative virus group (including control group,PQ group,PQU+TI group,UTI group) and P-gp siRNA group (including control group,PQ group,PQU+TI group,UTI group).Negative virus group:the cells were transfected into the blank virus;siRNA P-gp group:the cells were transfected with P-gp siRNA virus.HK-2 cells were routinely cultured.After 800 μmol/L PQ treatment,the changes of P-gp protein levels in the HK-2 cells were determined by Western-blot (WB).Then,transfected lentivirus bringing P-gp silent gene,the cell viability was determined by CCK-8 assay,the expression of P-gp in the cells after transfection was detected by WB and the concentration of PQ in HK-2 cells were measured by high performance liquid chromatography (HPLC).Results Compared with the normal control group,the P-gp expression of PQ group had no significantly changes (P＞0.05).Compared with the PQ group,the P-gp expression of UTI +PQ group significantly increased (P＞0.05).Compared with the corresponding control siRNA group,the P-gp siRNA group had no significantly changes in cell viability (P＞ 0.05).and significantly decreased in P-gp expression.Compared with the corresponding control siRNA group,the P-gp siRNA group had no significantly changes in PQ concentration in HK-2 cell (P＞0.05),but compared with P-gp siRNA PQ group,the PQ concentration of P-gp siRNA PQ+UTI group significantly decrease(P＜0.05).Conclusion UTI significantly reduced the accumulation of PQ in HK-2 cells and increased the viability of HK-2 cells in vitro may be not by increased P-gp activity.UTI could significantly reduce HK-2 cell injury induced by PQ in vitro and improve the survival rate of HK-2 cells.It may not be related to the up regulation of P-gp expression.