Objective To investigate the relationship between the expression of miR-455-3p and miR-141-3p levels in serum and the severity of acute pancreatitis(AP)in patients.Methods A total of 133 AP patients(AP group)who visited the People's Hospital of Dujiangyan from November 2021 to November 2023 were regarded as the study subjects.According to the severity of disease,AP patients were separated into a severe group(n=43)and a mild group(n=90).During the same period,135 healthy individuals who underwent physical examination in the hospital were regarded as the control group.Real-time fluorescence quantitative PCR method was applied to detect serum levels of miR-455-3p and miR-141-3p.Spearman correlation analysis was applied to analyze the correlation between serum miR-455-3p and miR-141-3p levels and acute physiology and chronic health evaluation Ⅱ(APACHE Ⅱ)and Ranson scores.Receiver operating characteristic(ROC)curve was applied to analyze the diagnostic value of serum miR-455-3p and miR-141-3p levels for severe AP.Multivariate logistic regression was applied to analyze the factors affecting the occurrence of severe AP.Results The serum miR-455-3p level(0.61±0.13)in the AP group was lower than that in the control group(1.12±0.21),while the miR-141-3p level(1.37±0.11)was higher than that in the control group(1.04±0.15),with significant differences(t=23.863,20.513,all P＜0.05).The serum miR-455-3p level(0.53±0.10)in the severe group was lower than that in the mild group(0.64±0.12),while the miR-141-3p level(1.51±0.14)was higher than that in the mild group(1.30±0.15),with significant differences(t=6.056,7.713,all P＜0.05).The serum amylase(AMS),lipase(LPS)levels,APACHE Ⅱ and Ranson scores in the severe group were higher than those in the mild group,and the differences were significant(t=2.227,2.290,18.267,11.259,all P＜0.05).Spearman correlation analysis results showed that the serum miR-455-3p of AP patients was negatively correlated with APACHEⅡ and Ranson scores(r=-0.702,-0.783,all P＜0.05),while miR-141-3p was positively correlated with APACHEⅡ and Ranson scores(r=0.787,0.734,all P＜0.05).ROC curve results showed that the AUCs of serum miR-455-3p and miR-141-3p levels alone and combination of severe AP were 0.848,0.822 and 0.919,respectively.And the combination detection was superior to single detection(Z=3.081,2.524,all P＜0.05).Multivariate Logistic regression analysis showed that miR-455-3p was a protective factor affecting severe AP,while miR-141-3p was a risk factor affecting severe AP(P＜0.05).Conclusion The decrease of serum miR-455-3p and the increase of miR-141-3p in AP patients are closely related to the severity of disease.

By reviewing the organization and implementation of “Hongsha-2021” Guangxi nuclear emergency joint exercises, this article summarizes the experience in the organization process and puts forward some thoughts and suggestions in order to improve the depth of provincial-level on-site and off-site joint exercises for nuclear emergency at nuclear power plants and further enhance the emergency response capacity of nuclear emergency organizations at all levels.