Cellular immunological reaction of the subjects after transplantation of deep-frozen (at -196℃) al-logenic bone grafts was studied using the sensitive LDH - release assay of morden immunological technique. The antigenicity of allogenic bone was definitely decreased after deep-freezing treatment and related to the time of treatment. In the experimental models the antigenicity of the deep-frozen allogenic bone was reduced to the same level as that of isogenic bone when it had been frozen at-196℃ for 7 days.

Objective To observe the clinical effects of prostaglandin E1 on liver cirrhosis patients and evaluate its security. Methods 68 cases with liver cirrhosis admitted in Luqiao hospital were randomly divided into experimental group(34 cases)and control group(34 cases)equally. Patients in control group were received normal therapy,while in experimental group were added prostaglandin E1 on basis of normal therapy. The efficacy and adverse reactions were observed and compared. Results Compared with before treatment,the average value of albumin in two groups were increased obviously,which in experimental group from (27.9 ±4.1)g/L to (36.5 ±4.3)g/L,control group from (27.8 ±4.0)g/L to (31.7 ±4.2)g/L,the differences were significant(P<0.05 ),and the difference between two groups was significant(P<0.05 ),too. The content of alanine aminotransferase and aspartase aminotransferase in two groups were decreased significantly(P<0.05 ),and experimental group was more lower than control group(P<0.05 ). The number of effcacy in experimental group was 28 and account for 82.35%,while in control group was 1 1 and account for 32.35%,the difference was significant(P<0.05). Conclusion Prostaglandin E1 can improve liver function,and has good clinical effects and high security in treatment with liver cirrhosis patients.

This paper introduced the concept of accumulation-dispersing method and its theoretical basis as well as clinical application in the treatment of gland diseases. With three diseases, which were the Sjogren’s syndrome, cystic hyperplasia of breast and benign prostatic hyperplasia, as clues, common characteristics from etiology, pathology and pathogenesis were elaborated from the anatomical features, pathological characteristics and meridian pathways for the gland diseases. The disease pathogenesis always belonged to“mass” and“knot” of traditional Chinese medicine (TCM). The detailed clinical applications were as follows. For the pattern of blood stasis, the treatment principle was to promote blood circulation and to resolve masses. For the pattern of phlegm, the treatment principle was to reduce phlegm and to resolve masses. For the pattern of heat, the treatment principle was to clear heat, to relieve toxin and to resolve masses. For patients of“tumor” or“phthisis”, the treatment principle was to strengthen vitalqi and to eliminate stagnation. Worm medicine should also be combined during accumulation-dispersing. This paper provided referential ideas and methods for TCM treatment of gland diseases.

Objective:To construct eukaryotic expressing vector of the full length coding sequence of Bad gene and to express the gene in the basal cell carcinima A431 cells.Methods:Bad gene was amplified from Hela cell line by RT-PCR and the fragment of the cDNA was cloned into eukaryotic expressing vector pcDNA3.1-myc by ligating the fragment into XhoI and EcoRI site.The recombinant plasmid pcDNA3.1-myc-Bad was identified by DNA sequencing and restriction enzyme analysis.The gene transfection mediated by lipofectin was used to introduce the eukaryotic expressing vector of pcDNA3.1-myc-Bad into human basal cell carcinima A431 cells. After selection with G418, resistant colonies were obtained.Trasfection efficiency was identified by Western blot and SABC-FITC assay.Cell proliferation was examined by cell counting and colonogenic assay after transfection.Results:A 500 bp DNA fragment was amplified with RT-PCR.Sequence and restriction enzyme analysis showed that the recombinant plasmid pcDNA3.1-myc-Bad was constructed successfully.In human basal cell carcinima cell line A431 Bad gene was expressed.The cell proliferation was inhibited by 62.6% and colonogenesis by 39.9% by the transfection of the gene.Conclusion: Human Bad gene was successfully cloned.Transfection of basal cell carcinoma cells with the gene may inhibit the cell proliferation and colonogenesis.

objective: To evaluate the embolization effect of 5 Fu gelatin microsphere(FGM) in oral and maxillofacial region.Methods: FGM was prepared with drug load of 12% and encapsulation of 85%. After irridiated with 60 Co, FGM was suspended into 76% of meglumine diatrizoate, and then perfused into external maxillary artery and lingual artery in 8 dogs under X ray observation. 4 dogs were used in each artery embolization. Blood flow velocity and volume in the arteries were tested at different time after embolization; all the data were analyzed statistically. The sections of embolized tissue at different time were examined microscopically. Results: Before and 1 h, 48 h and l month after ombolization the blood flow volume(ml/min) in external caroted artery was 43?5.0, 31.0?4.5, 26.5?4.0 and 35.0?30; that in external maxillary artery 5.0?1.5, 0.5?1.0, 2.5?1.0 and 3.0?1.5; in ligual artery 3.5?1.0, 0.5?0.5, 0 and 0; in internal maxillary artery 17.0?4.0, 19.5?4.0, 19.0?4.5 and 21.0?3.5, respectively. Stuff of the cavity of the corresponding arteries by FGM were found with histological examination. Conclusion: FGM can embolize artery completely.

Objective: To analyse the acuracy of color doppler ultrasound examination in the measure- ments of blood vessels in tissue flaps. Methods: 110 pieces of associated blood vessel in tissue flaps in 64 cases were measured with color doppler ultrasound examination before operation. The quality of blood vessel, its diameter, blood stream filling, the vascular pedicle length and its depth were measured. The results were checked and compared with the findings in operation. Results: Of all 110 pieces of associated blood vessel evaluated by doppler, the accurate ratio confirmed by the finding in operation was 100%. The inner diameter of 38 pieces of blood vessel measured in operation was coincident with that measured by doppler before operation, or with little difference. Conclusion: The color doppler ultrasound examination is a reliable method for evaluating the blood vessel both of the donor and host areas in maxillofacial reconstruction surgery with tissue flap.

Objective: To analyses the characters, diagnostic methods and therapeutic principles of non-neoplastic diseases of parotid gland. Methods:179 cases (84 famale and 95 male) of non-neoplastic disease treated in our hospital from 1985 to 2000 were reviewed and analysed clinically.Results: The patients were at the age of 1.5～76 years old (44.6 for average) with the case histry of 1～10 years. Among the 179 cases,66 were of chronic parotitis,48 lymphoid tuberculosis,24 benign lympho-epithplial lesion,22 lymphadenitis and lymphadenovarix and 19 other benign lesion.158 cases were treated by operation and the diagnosis of them was confirmed by pathological examination after operation;21 were clinically diagnosed and treated with drugs. Conclusion: Non-neoplastic diseases of parotid gland are easily confounded with neoplastic diseases. Surgically resection is an effective method for diagnosis and treatment.

Objective To assess the diagnostic value of three-dimensional(3D) helical CT imaging in the cases of complex maxillofacial fractures and to discuss the clinical usefulness of 3D to surgeons.Methods 26 patients with trauma suspected of having complex facial fractures were examined with thin-slice CT scan and 3D reconstruction.Results The location,shape and displacement of fragment of complex maxillofacial fractures were demonstrated steroscopically by three-dimensional images reconstructed from helical CT.Conclusion 3D helical CT imaging can provide valuable information in demonstrating the space relationships of maxillofacial fractures.

Objective:To evaluate 2D ultrasonography(2DUS), color Doppler flowing imaging(CDFI) and color Doppler energy (CDE) in the differential diagnosis of benign and malignant tumors of salivary glands.Methods:According to the characters of 2DUS and CDFI examination, benign and malignant tumors in 142 cases of salivary glands were distinguished. The results of ultrasound examination and postoperative biopsy were checked and compared. Meanwhile the same tumor's blood flow information and hemodynamics index were compared. Results:In 2DUS examination, the inter echo and post echo of benign and malignant were different. In CDFI examination, the blood flow form could be displayed clearly. Besides Warthin tumors, the blood supply ranking of benign tumors was usually levelⅠor Ⅱ-(78.7%), and that of malignant tumors was levelⅡ+ or Ⅲ(80.0%). The artery's peak systolic velocity(PSV) of malignant tumors was higher than that of benign tumors. The rates(%) of sensitivity, specificity,accuracy,false negative, false positive, positive predicting and negative predicting of 2DUS were 70.2,87.4,81.7,29.8,12.6,73.3 and 85.6,those of CDFI 80.9,65.2,70.4,19.1,34.7,53.0 and 87.3,those of the integrated usage of ultrasonic methods 89.3,90.5,90.1,10.6,9.5,82.4 and 94.5 respectively.Conclusion:With the integrated usage of ultrasonic methods, higher accuracy of differential diagnosis for benign and malignant salivary glands tumors may be obtained.

Objective To analyze the clinical characteristic and provide therapeutic techniques of maxillofacial trauma in the elderly. Methods Geriatric maxillaofacial trauma patients admitted to our hospital from January 1992 to December 2002 was retrospectively reviewed. Data were collected according to etiology, location, comorbidities, associated injuries, therapeutic methods and the outcome. The patients were compared with a control group consisting of 200 young and adult patients admitted at the same period. Results For the 126 geriatric trauma patients, the major causes were traffic accidents(61.1%) and violence assault(25.4%). A large proportion of facial injuries occurred on the lower part of the face. 69.1% of the patients presented with preexisting diseases. Most of the fractures were not treated(35.7%). The length of hospitalization and ICU stay were relatively longer when compared with the control group(P