Objective To investigate the expression level of CRIF1 in human leukemic Jurkat T-cell line cocultured with primary leukemic bone marrow stromal cells.Methods RT-PCR was used to study the expression level of CRIF1 in human leukemic Jurkat T-cell line cocultured with primary leukemic bone marrow stromal cells in vitro. Results After coculture with leukemic bone marrow stromal cells, the expressions of CRIF1 and Gadd45 mRNA in Jurkat cells were significantly higher than the control.Conclusion High mRNA levels of CRIF1 might be associated with the G0/G1 phage arrest in human leukemic Jurkat T-cell line cocultured with primary leukemic bone marrow stromal cells.

0.05) while that in groups A and C differed from group B ( P

Objective To screen and analyze the differentially expressed genes of Jurkat cells cocultured with bone marrow stromal cells(BMSCs) from leukemia patients.Methods Suppression subtractive hybridization(SSH) was employed to establish subtracted cDNA library of differentially expressed genes in Jurkat cells cocultured with BMSCs from leukemia patients in vitro.The cDNA fragments were sequenced and analyzed.Results The differentially expressed gene cDNA library of Jurkat cells cocultured with BMSCs from leukemia patients in vitro was developed by SSH.Primary screening was done by reverse northern hybridization.Thirty up-regulated and 22 down-regulated cDNA fragments were isolated and sequenced,and sequence homology was performed by BLAST in GenBank.These genes were related to cell cycle regulation,cellular apoptosis and energy metabolism.The information of differentially expressed genes in Jurkat cells implied that their secretion function,cell cycle and apoptosis were affected by BMSCs from leukemia patients.Conclusion BMSCs from leukemia patients might influence gene expression of Jurkat cells.The differentially expressed genes might be significantly associated with the protection of BMSCs from leukemia patients on Jurkat cells during Daunorubicin exposure.

Objective:To observe the effect of emotional resilience training on compassion fatigue and psychological resilience of intensive care unit nurses.Methods:A total of 70 intensive care unit nurses were randomly divided into experiment group and control group, each of 35 cases. Control group nurses were given routine psychological guidance intervention, in addition, the experiment group was carried out 8 weeks of emotional resilience training. The effect of intervention was assessed by Chinese version of the compassion fatigue scale and Connor-Davidson resilience scale before and after intervention.Results:After intervention, the average scores of compassion satisfaction, secondary traumatic stress and total compassion fatigue scores were (26.46±4.99)pionts, (17.80±4.05)pionts and (69.14±5.70)pionts, significantly lower than in the control group (28.86±4.14)pionts, (20.11±4.38)pionts and (75.51±10.32)pionts, the difference was statistically significant ( t value was 2.190, 2.296, 3.197, P<0.05). After intervention, the average scores of tenacity, power, optimism and total resilience scores were (34.23±6.06)pionts, (25.77±5.01)pionts, (14.31±3.22)pionts and (74.31±9.55)pionts, significantly higher than in the control group (30.86±6.23)pionts, (23.31±3.29)pionts, (12.11±2.04)pionts and (66.29±7.28)pionts, the difference was statistically significant ( t value was 2.295-3.956, P<0.05). Conclusion:Emotional resilience training can effectively reduce the compassion fatigue and improve psychological resilience of intensive care unit nurses.

Objective To investigate the effect of on serum and urine MCP -1 secretion in patients with Stage IV Type 2 diabetic nephropathy treated by lipoic acid .Methods We enrolled 76 diabetic nephropathy patients ,who were randomly divided into two groups . Patients in group T (24 males and 14 females) were treated by lipoic acid 0.6 gram per day.Those in group C (22 males and 16 fe-males) were treated by routine drugs.The serum creatinine (Scr), urea nitrogen (BUN), fasting blood sugar(FBS) and 24h urinary pro-tein,serum and urine MCP -1 secretion were measured at the experiment onset and 3 weeks later.Results There was no significant difference in the serum creatinine , urea nitrogen, fasting blood sugar, HbA1c between the two groups neither at the experiment onset nor after 3 weeks.Compared to experiment onset , 24h urine protein (Tp/24h), serum and urine MCP-1 secretion were all significantly de-creased (P<0.05) in group T after 3 weeks.Compared to group C (2.41 ±0.91g/24h, 91.45 ±33.41pg/ml, 114.78 ±36.35pg/ml), all the levers in group T (1.89 ±0.72g/24h, 39.50 ±13.68pg/ml, 63.41 ±19.57pg/ml) was significantly decreased (P<0.05) after 3 weeks.There was a positive correlation between the serum , urine MCP-1 levels and Tp/24h (r=0.572, P<0.05;r=0.697,P<0.05).Conclusion Lipoic acid can reduce urine protein excretion in diabetic nephropathy patients , maybe by decreasing serum and u-rine MCP-1 secretion .

BACKGROUND:There are few clinical control ed trials about the clinical effects in patients with pertrochanteric femur fractures after treatment with short or long proximal femoral nail antirotation.
OBJECTIVE:To compare the clinical outcomes in patients with AO/ASIF-A1/2 pertrochanteric femur fractures after treatment with short or long proximal femoral nail antirotation.
METHODS:A total of 98 patients with AO/ASIF-A1/2 pertrochanteric femur fractures were treated by proximal femoral nail antirotation. They were divided into two groups according to the type of proximal femoral nail antirotation:short nail group (n=50) and long nail group (n=48). The operative time, blood loss, and hospital stay were recorded in both groups. In fol ow-up, fracture healing time, imaging and clinical complications were evaluated. In the final fol ow-up, Harris hip score was used to evaluate functional recovery.
RESULTS AND CONCLUSION:Compared with the short nail group, operative time was shorter and blood loss was less in the long nail group (P<0.05). No significant difference in hospital stay was detected between the short nail and long nail groups (P>0.05). Average fol ow-up periods were respectively (15.8±6.4) months and (16.2±5.7) months in the long nail and short nail groups.“Cutting-out”or infection occurred in five patients in the long nail group and three in the short nail group. Besides above-mentioned patients, the remaining patients in the two groups achieved fracture healing. No significant difference in average fracture healing time was detected between groups (P=0.588). In the final fol ow-up, no significant difference in Harris hip score was detectable in the two groups (P=0.204). The incidence rates of total postoperative complications in the long and short nail groups were 31.2%and 16.0%, respectively (P=0.075). Results suggested that no differences in the union and complication rates between the two groups were identified, suggesting that long nails offer no advantage compared with short nails for stabilizing AO/ASIF-A1/A2 pertrochanteric femur fractures.

Objective To study the anti-depressive mechanisms of acupuncture by investigating the effects of acupuncture on depressive behaviors and amino-neurotransmitter in hippocampus in rats treated with chronic mild unpredicted stresses.Methods Thirty adult SD rats were randomly divided into a normal group,a depressive model group and an acupuncture group,with 10 rats in each group.Separated feeding,long-term unpredictable and middle stimulation stress were used for development of depression rat model.At the same time,the third group was treated by acupuncture.Autonomic behavior was measured with Open Field test and concentration of amino-neurotransmitter in hippocampus was measured by high performance liquid chromatography coupled with fluorescence detection.Results Compared with the normal group,autonomic activity of the rats and the concentration of GABA were significantly reduced in the model group,while the concentration of Glu was significantly increased(P0.05).Conclusion Acupuncture could reverse the depressive behaviors of rats caused by middle stimulation stress,and its anti-depressive mechanisms is related to the function of regulating the concentration of amino-neurotransmitter in hippocampus.

Objective To clarify the risk,treatment and preventive measurement of transfusion related acute lung injury (TRALI) occurred in HLA half-matched hematopoietic stem cell transplantation. Methods A case of TRALI occurred in HLA half-matched hematopoietic stem cell transplantation was analyzed,including the clinical characteristics,the laboratory and instrumental examination,the treatment measure and prognosis,and then the associated literature was reviewed.Results Owing to the blood products(fresh platelet) transfusion during transplantation,the patient got TRALI. And after active rescue,the patient eventually died due to the worsen condition.Conclusion The risk of TRALI is very high in HLA half-matched hematopoietic stem cell transplantation since the blood products transfusion is inevitable,so the effective and timely treatment and preventive measurement are necessary.

Objective To study the influence of bone marrow microenvironment on leukemic cells during chemotherapy and the influence on leukemic cells' drug-resistance and anti-apoptosis. Methods Normal bone marrow stromal cells were isolated using Percoll, and then were cocultured with human acute lymphocyte leukemic cell line Jurkat cells in vitro. Apoptosis percentage of Jurkat cells labelled with Annexin V/PI were detected by FCM. Results Apoptosis percentage of Jurkat cells cocultured with bone marrow stromal cells for 24 hours was lower than those maintained in suspension (P

Objective To investigate the ABO blood type and sepsis complicated with acute kidney injury,to provide the basis for prevention and treatment.Methods A total of 130 patients with sepsis from renmin hospital of Wuhan University intensive care unit from january 2015 to december 2015 were enrolled in this study,divided into complicated AKI group (64 patients in the observation group) and non-complicated AKI group (66 patients in the control group),analyzed two groups of general data,laboratory indicators,multivariate logistic regression analysis was used to screen out the risk factors for AKI in patients with sepsis.Results A total of 64 patients with AKI were collected from the observation group and 66 patients with non-AKI in the control group,the age of the observation group was higher than that of the control group (65.7 ± 13.1 years old vs 58.5 ± 15.4 years old,P =0.005),male proportion was higher than control group (76.6％ vs 56.1％,P =0.014),drop calcium pigment original quantitative was higher than control group (28.1 ± 21.0pg/L vs 21.1 ± 13.61μg/L,P =0.026),positive blood culture rate was higher than in the control group (30.2％ vs 15.3％,P =0.006).There was no significant difference in ABO blood group distribution between the two groups (P =0.825).The levels of white blood cell count,C-reactive protein and partially activated thrombin were higher in the observation group than in the control group,the platelet count and albumin level were lower than those in the control group,the difference was not statistically significant.The risk factors associated with the incidence of sepsis with AKI were analyzed by multivariate analysis of the logistic regression model:age(P =0.021,OR =0.965),gender (P =0.003,OR =5.321),calcitonin-original(P =0.047,OR =0.975),positive blood culture (P =0.002,OR =1.009),comparison of type A blood and type O blood (P =0.037,OR =5.409) were associated with sepsis complicated with AKI.Conlusion Type A blood and sepsis with AKI associated with the existence of independent correlation,type A blood may increase the risk of sepsis with AKI.