MTA1 is a composition of Nurd,which may deacetylate histone and non-histone to affect gene transcription and protein expression. Since it is often overexpressed in many tumors and closely related to invasion,metastasis and prognosis of the malignancy, MTA1 might be exploited as a tumor marker for clinical application. This article reviews the structure and function of MTA1 and some new research progress on the tumor metastasis related to MTA1.

Objective:To investigate the sensitivities and specificities of high-frequency color doppler ultrasonography, thin layer CT enhancing scan and 99m Tc-MIBI biphasic scintigraphy in diagnosis of secondary hyperparathyroidism(SHPT)in uremia patients.Methods: High-frequency color doppler ultrasonography, thin layer CT enhancing scan and 99m Tc-MIBI biphasic scintigraphy were performed in 35 uric patients to detect the hyperplastic parathyroid glands. Then biopsies with thin needles were conducted with B ultrasound instruction in hyperplastic glands to determine the sensitivities and specificities of the above techniques and free combination of each 2 of them in diagnosis of SHPT. Results: The sensitivities /specificities of high-frequency color doppler ultrasonography, thin layer CT enhancing scan and 99m Tc-MIBI biphasic scintigraphy were 73%/95%, 75%/93% and 89%/98%, respectively; the sensitivity/specificity of 4 combinations: high-frequency color doppler ultrasonography plus CT, high-frequency color doppler ultrasonography plus 99m Tc-MIBI biphasic scintigraphy, CT plus 99m Tc-MIBI biphasic scintigraphy,and high-frequency color doppler ultrasonography plus CT plus 99m Tc-MIBI biphasic scintigraphy were 84%/90%, 93%/93%, 95%/93%, and 100%/90%, respectively. Conclusion: The sensitivity of 99m Tc-MIBI biphasic scintigraphy SHPT is higher than that of high-frequency color doppler ultrasonography and CT, but the specificity is not significantly different 3 photographic techniques. However, combination of high-frequency color doppler ultrasonography and 99m Tc-MIBI biphasic scintigraphy is simple and sensitive and can be regarded as the first choice for diagnosis of SHPT in uremia patients.

Objective To investigate the effects of liver function and tumor markers by nucleoside analogues Entecavir on patients with liver cirrhosis after hepatitis B.Methods 90 patients with liver cirrhosis after hepatitis B were selected, according to the different drugs were divided into experimental group and control group.Liver function and levels of tumor markers were compared after experiment.Results Two groups of patients with male to female ratio, average age, course of disease, no significant difference in general data of hepatitis B virus DNA content, comparable (P>0.05);Compared with the control group, the experimental group HBV DNA level is low, the negative rate was significantly higher (P<0.05);Compared with the control group, the experimental group ALT, AST and TBiL levels were significantly increased(P<0.05), ChE, AlB were significantly decreased (P<0.05);Compared with the control group, the experimental group CEA, AFP, CA125 and lower CA199 levels (P<0.05).Conclusion Nucleoside drugs can significantly improve liver function in patients with liver cirrhosis after hepatitis Band tumor markers indicators, and it is significance for treatment of liver cirrhosis after hepatitis.

To explore the influence of low light on the synthesis of carotenoids, chlorophyll and the adaptability of transgenic plants with tomato Solanum lycopersicon L. GGPS2 gene, we constructed a vector containing a GGPS2 gene with green fluorescent protein (GFP) as report gene under the control of a cauliflower mosaic virus 35S promoter and introduced it into tobacco Nicotiana tabacum L. cv. Wisconsin 38 by Agrobacterium tumefaciens-mediated transformation. PCR analysis of the DNA from kanamycin resistant tobacco indicated that the transgenic tobacco containing the nptII gene, SlaGGPS2 gene and without contamination of Agrobacterium. We also detected the root tip of kanamycin resistant tobacco showing characteristic fluorescence. The contents of carotenoid, chlorophyll and photosynthesis of transgenic tobacco increased in comparison with wild tobacco after low light treatment. In addition, leaf mass per unit area, total dry weight, ratio of root to shoot in transgenic tobacco were all higher than that of the wild tobacco, which proved that the transgenic tobacco could increase the accumulation of biomass and promote it transport to root. The transgenic tobacco with SlaGGPS2 gene can increase the contents of carotenoid, chlorophyll, enhance the photosynthetic rate, promote the biomass accumulation and its distribution to root. Hence, the transgenic tobacco with SlaGGPS2 gene had increased low light tolerance and the SlaGGPS2 gene maybe can be used in other crops.
Agrobacterium tumefaciens ; Carotenoids ; analysis ; Chlorophyll ; analysis ; Gene Expression Regulation, Plant ; Genetic Vectors ; Light ; Lycopersicon esculentum ; genetics ; Photosynthesis ; Plants, Genetically Modified ; metabolism ; radiation effects ; Tobacco ; metabolism ; radiation effects

Under physiological conditions, many vital functions of the body are controlled by transient release of bioactive substances at a specific time and site. Based on the circadian rhythm character of disease and chronotherapeutic conceptions, pulsatile delivery system has been designed to achieve optimal therapeutic effect and reduce the toxic and side-effect. In recent years, more and more studies are focused on the pulsatile multiparticulate drug delivery system. Pulsatile multiparticulate system possesses many benefits, such as no risk of dose dumping, predictable gastric emptying, flexible release patterns and increased bioavailability. Based on these premises, the aim of this review is to summarize the major design methods of pulsatile multiparticulate and the research progress.

BACKGROUND:Traditional distal femoral fixation plate screw breakage is relatively common. Designing good anatomical and attached fixation system is the key for clinical application.
OBJECTIVE:To perform finite element analysis in two states of motion of the minimal y invasive distal femoral fixation system, compare stress distribution of different parts in the same fixed way, different fixed methods and the same fixed parts of different motion states.
METHODS:Imaging data of a 34-year-old male patient weighing 68 kg with 33-C1 type fracture of distal femur were selected. CT data were input into Mimics 16.0 for reconstruction. PRO-E software was used to establish minimal y invasive internal fixation system with distal femoral locking plate. Data were introduced into reconstructed models of distal femur fracture in Mimics for grid division. Data were introduced into Ansys products 11.0 to construct finite element model, fix the surface of distal femur, and loaded 340 N on greater trochanter of femur. Stress distribution of each plate, screw hole and screw tail was analyzed in each group. Stress at the same region was compared in flexion and extension movement states.
RESULTS AND CONCLUSION:(1) Finite element models of anatomic locking plate for distal femur fracture fixation were successful y established, total y 43 536 units, 41 256 nodes. (2) With the steel segment gradual y down (S1-S5), the stress gradual y increased. A1-A5 with the increase in the number of screws, the stress gradual y increased, but A6 suddenly decreased. (3) According to the cloud atlas of stress, these were wel distributed except A1. From distal end to extremity of screw, the stress of screws increased. Among corresponding segments, significant differences in stress around the nail holes and steel segment stress were detected. Moreover, the steel stress was greater than the stress of corresponding segment of screw hole. (4) Results suggest that using anatomical locking plate and minimal y invasive internal fixation system for distal femur fracture in a variety of fixed modes and moving conditions, the stress of each part is less than the yield strength of the titanium al oy screw, so the fixed system wil not produce instantaneous deformation or fracture.

OBJECTIVE To clarify the features of nosocomial infections for patients with multiple myeloma(MM).METHODS One hundred and seventy eight cases of MM treated in our hospital from Oct 1982 to Dec 2006 were analyzed retrospectively.RESULTS Eighty five patients(47.8%) suffered from nosocomial infections.The nosocomial infection took place more frequently on respiratory tract.Elder,granulocytopenia,hypoproteinemia,severe anemia,and diabetes were the risk factors.Totally 91 pathogens were isolated.Gram-negative bacilli accounted for 45.1%,and were the major pathogens.Fungi accounted for 37.4% and Gram-positives accounted for 17.6%.The ESBLs producing strains accounted for 31.6% in Klebsiella pneumoniae and Escherichia coli.Vancomycin resistant strains were found.The top one of fungi presented organism was the Candida albicans.CONCLUSIONS Nosocomial infections for patients with multiple myeloma has a high incidence.There are many risk factors.The resistance of commonly encountered bacteria to antimicrobial agents is a serious problem.Immunity protection and the rational use of antimicrobial agents should be emphasized.

A novel molecularly imprinted polymer with adequate attractability for Schistosoma japonicum miracidia was prepared.When adulterated with polyvinyl alcohol(PVA),the fabricated film with good swelling property was formed which can suspend on water and slowly release XF(a chemical to be published).This reusable film can well attract Sch-istosoma japonicum miracidia,and hopefully be used in the prevention of schistosome infection.

Pharmaceutical preparations can directly affect the administration methods and therapeutic effects of drugs , which is a priority for the research and development of the military specialized medicament .Foreign armies started pharma-ceutical formulation research very early , and some of their research concepts and strategies are worth learning from .In this paper , dosage forms were used as the classification factor and several formulations with distinct military characteristics were described in detail .The features of military specialized medicament were analyzed from the perspective of pharmaceutics , based on which future development in the formulation of military specialized medicament was predicted .