With the emergence, development and innovation of minimally invasive surgical and laparoscopic technologies, minimally invasive technology has been gradually applied and promoted in different fields of surgery, and surgical indications have been constantly expanded. Robot-assisted surgical system has become a novel research hotspot due to its precision and minimal invasiveness. At present, robot-assisted surgical system can be applied in complex tumor surgery. How to apply robot-assisted surgery in the field of liver transplantation, especially in the living donor liver hepatectomy, has become a new research direction, which is also a challenge facing multiple scholars. In this article, the advantages of robot-assisted surgery, current status and major difficulties of robot living donor liver hepatectomy were reviewed, and the future of robot living donor liver hepatectomy was predicted, aiming to provide reference for promoting the application of robot-assisted surgery in clinical liver transplantation.

Objective To investigate the expression of chemokine receptors CCR4,CCR6,CXCR3 on Th17 cell,and analyze the correlation between chemokine receptors and serum biochemical parameters of liver function test and hepatitis B virus (HBV) DNA load in peripheral blood of chronic hepatitis B (CHB) patients.Methods Thirty patients with CHB (CHB group) and 15 healthy persons (control group) were enrolled in the study.CCR4,CCR6,CXCR3 expression levels on Th17 cell were assayed by flow cytometry.The correlation between chemokine receptors and alanine aminotransferase (ALT),total bilirubin (TBil),and HBV DNA load were analyzed using Pearson' s correlation analysis,respectively.Results CCR4,CCR6,CXCR3 expression levels on CD4 + Th17 cell in CHB group were higher than that of control group.Moreover,the difference between them was statistically significant (P ＜ 0.05).CCR4,CCR6 expression levels on CD8 + Th17 cell in CHB group were higher than that of control group (P ＜ 0.05).Although CXCR3 expression level on CD8 + Th17 cell in CHB group was higher than that of control group,there was no significant difference between them (P ＞ 0.05).CCR4,CCR6 expression levels were positively correlated with serum ALT,HBV DNA load,respectively (P ＜ 0.05),but were not correlated with TBil (P ＞ 0.05).Conclusion Expression levels of chemokine receptor on Th17 cell were increased in patients with CHB and were positively associated with degree of liver inflammation.Therefore,CCR4,CCR6 expression on Th17 cell might be involved in liver injury resulted from Th17 cell.

Objective To investigate the effects of single-port laparoscopic extraperitoneal liga-tion on surgical indexes,intestinal mucosal barrier function and recurrence rate in children with pedi-atric inguinal hernia.Methods A total of 86 children with pediatric inguinal hernia in the hospital from February 2019 to June 2021 were randomly divided into control group(n=43)and study group(n=43).The study group performed single-port laparoscopic extraperitoneal ligation,while the con-trol group received traditional open surgery.The ratio of urinary lactulose to mannitol(L/M)was measured by high-performance liquid chromatography;serum diamine oxidase(DAO)level was de-tected by enzyme-linked immunosorbent assay;serum D-lactic acid level was detected by enzymatic spectrophotometry;pain was assessed by the Visual Analogue Scale(VAS);surgical indicators,com-plications and postoperative recurrence rate were compared between the two groups.Results Com-pared with the control group,the study group had significant reduction in operation time,intraopera-tive blood loss,time to first flatus,and postoperative hospital stay(P＜0.05);in both groups,the VAS score,L/M,DAO level and serum D-lactic acid level decreased significantly after operation,and these indexes after operation in the study group were significantly lower than those in the control group(P＜0.05);the incidence of complications in the study group was 4.65％(2/43),which was significantly lower than 20.93％(9/43)in the control group(P=0.024).Conclusion Single-port laparoscopic extraperitoneal ligation is benefit for the treatment of children with pediatric inguinal hernia,which has advantages of fewer bleeding,less pain,fewer complications,faster recovery of intestinal mucosal barrier function,and lower recurrence rate.

Objective To investigate the effects of single-port laparoscopic extraperitoneal liga-tion on surgical indexes,intestinal mucosal barrier function and recurrence rate in children with pedi-atric inguinal hernia.Methods A total of 86 children with pediatric inguinal hernia in the hospital from February 2019 to June 2021 were randomly divided into control group(n=43)and study group(n=43).The study group performed single-port laparoscopic extraperitoneal ligation,while the con-trol group received traditional open surgery.The ratio of urinary lactulose to mannitol(L/M)was measured by high-performance liquid chromatography;serum diamine oxidase(DAO)level was de-tected by enzyme-linked immunosorbent assay;serum D-lactic acid level was detected by enzymatic spectrophotometry;pain was assessed by the Visual Analogue Scale(VAS);surgical indicators,com-plications and postoperative recurrence rate were compared between the two groups.Results Com-pared with the control group,the study group had significant reduction in operation time,intraopera-tive blood loss,time to first flatus,and postoperative hospital stay(P＜0.05);in both groups,the VAS score,L/M,DAO level and serum D-lactic acid level decreased significantly after operation,and these indexes after operation in the study group were significantly lower than those in the control group(P＜0.05);the incidence of complications in the study group was 4.65％(2/43),which was significantly lower than 20.93％(9/43)in the control group(P=0.024).Conclusion Single-port laparoscopic extraperitoneal ligation is benefit for the treatment of children with pediatric inguinal hernia,which has advantages of fewer bleeding,less pain,fewer complications,faster recovery of intestinal mucosal barrier function,and lower recurrence rate.

Pancreatic cancer is a common malignant tumor of the digestive tract， and the incidence of pancreatic cancer has been gradually increasing in recent years. The main pathological types of pancreatic cancer include ductal adenocarcinoma， small gland carcinoma， and acinar cell carcinoma， among which duct adenocarcinoma is the most common pathological type. Multidisciplinary diagnosis and treatment （MDT） is a new diagnostic and therapeutic mode for pancreatic cancer， and different medical teams， including hepatobiliary surgery， gastroenterology， endocrinology， radiology， and radiotherapy， are gathered to discuss the conditions of patients and develop treatment regimens and subsequent management measures， so as to determine the optimal individualized treatment regimen. With the continuous clarification and development of the concept of precision therapy and the discovery of related gene mutations， promising prospects and individualized treatment have been achieved for the treatment of pancreatic cancer， thereby providing opportunities for improving the treatment outcome of patients. This article analyzes the interaction between different disciplines under the model of MDT， discusses the development trend and directions of the treatment regimens for pancreatic cancer， summarizes the current research work for pancreatic cancer， and proposes the future research directions for MDT in pancreatic cancer.

Objective: In this study, black tea and Citrus maxima (BT-CM), yellow tea and C. maxima (YT-CM), green tea and C. maxima (GT-CM) as subjects, the active ingredient content and antioxidant activity of three tea and C. maxima (T-CM) were analyzed. The effects of three T-CMs on apoptosis of liver cells in vitro and its mechanism were further explored. Methods: National standard method and HPLC were used for active ingredient analysis. MTT, cell flow cytometry and Western blot were used to analyze the effects of three T-CMs on cell proliferation, apoptosis, and its underlying molecular mechanism. Results: The content of tea polyphenols, free amino acids, ratio of polyphenols and amino acids, ester catechins, non-ester catechins and caffeine in YT-CM and GT-CM was significantly higher than that of BT-CM. The in vitro antioxidant capacity of YT-CM and GT-CM was also significantly stronger than that of BT-CM. Three T-CMs had the effects of inhibiting proliferation, arresting cell cycle and inducing apoptosis in HepG2 and Bel7402 cells, especially YT-CM and GT-CM. Western blot analysis showed three T-CMs activated PI3K/AKT/mTOR signaling pathway and regulated the expression levels of apoptosis-related proteins Bax, Bcl-2 and Caspase-3/9. YT-CM and GT-CM had better ability to change the signal pathway than BT-CM. Conclusion: In short, T-CMs, which combined different degrees of fermentation tea with C. maxima, were rich in nutrients and biologically active substances. T-CMs, especially YT-CM and GT-CM, are healthy drinks that help to prevent and treat liver cancer.

The mammalian central nervous system (CNS) is considered an immune privileged system as it is separated from the periphery by the blood brain barrier (BBB). Yet, immune functions have been postulated to heavily influence the functional state of the CNS, especially after injury or during neurodegeneration. There is controversy regarding whether adaptive immune responses are beneficial or detrimental to CNS injury repair. In this study, we utilized immunocompromised SCID mice and subjected them to spinal cord injury (SCI). We analyzed motor function, electrophysiology, histochemistry, and performed unbiased RNA-sequencing. SCID mice displayed improved CNS functional recovery compared to WT mice after SCI. Weighted gene-coexpression network analysis (WGCNA) of spinal cord transcriptomes revealed that SCID mice had reduced expression of immune function-related genes and heightened expression of neural transmission-related genes after SCI, which was confirmed by immunohistochemical analysis and was consistent with better functional recovery. Transcriptomic analyses also indicated heightened expression of neurotransmission-related genes before injury in SCID mice, suggesting that a steady state of immune-deficiency potentially led to CNS hyper-connectivity. Consequently, SCID mice without injury demonstrated worse performance in Morris water maze test. Taken together, not only reduced inflammation after injury but also dampened steady-state immune function without injury heightened the neurotransmission program, resulting in better or worse behavioral outcomes respectively. This study revealed the intricate relationship between immune and nervous systems, raising the possibility for therapeutic manipulation of neural function via immune modulation.