OBJECTIVE:To investigate the feature and tendency of the application of antihypertensive drugs in our hospital.METHODS:The utilization of antihypertensive drugs in our hospital from Jun.2004 to Dec.2006 was analyzed statistically in respect of the consumption sum and DDDs etc.RESULTS:The first line drugs used most frequently in our hospital were beta receptor antagonists,AGT Ⅱ antagonists,ACEI,a calcium antagonist etc.Leading the first place on the list of DDDs was indapamide because of its good safety and efficacy.Mesylate amlodipine,felodipine,irbesartan,perindopril,metoprolol tartrate,indapamide,bisoprolol fumarate,losartan/hydrochlorothiazide ranked among the first 10 places in either DDDs or consumption sum.CONCLUSION:The antihypertensive drugs are characterized by complicated categorization,wide variety,and widespread clinical application,and the use of which in our hospital is basically rational.

0.25. Conclusions Modified Stamey bladder neck suspension is an effective treatment for type Ⅰ female stsees urinary incontinence.

Objective To investigate the pathological changes of cardiac collagen in the elderly patients with hypertensive left ventricular hypertrophy(LVH)and the difference of collagen among the groups of essential hypertension(EH),diabetes (T2DM) under the condition of the same stage,providing pathological foundation of clinical treatment for curing diseases and the early heart protection.Methods A retrospective study was performed in 140 cases over 65 years old of EH and normal cases as control out of 3520 consecutive autopsied cases from 1954 to 2001 in Chinese People's Liberation Army (PLA) General Hospital.The pathological changes of collagen were studied with hematoxylin and eosion(HE) staining,Sirius Red F3B (SR)and immunohistochemistry staining.Myocardial interstital collagen deposition was observed with light microscope and polarization technique.Quantitative measurements on the myocardial collagen volume fraction (CVF),CVFS of type Ⅰ and type Ⅲ,and the rate of collagen type Ⅰ to type Ⅲ (Ⅰ/Ⅲ) were determined with videodensitometry.Results The CVF(6.32％ ± 0.92％) and CVF Ⅰ (5.93％ ± 0.94％)were increased significantly in EH LVH Ⅰ group (P ＜0.01) ; but,CVF Ⅲ (2.23％ ±0.38％) and Ⅰ/Ⅲ (2.69 ±0.42) did not change significantly in EH Ⅰ group compared with the control group; The changes in the aforementioned four items were increased significantly in EH LVH Ⅱ and EH LVH Ⅲ groups (P ＜0.01)compared with the control group.The CVF and CVF-Ⅰ were decreased significantly(P ＜ 0.01) ; but CVF-Ⅲ and Ⅰ/Ⅲ did not change significantly in DM LVH-I group compared with the EH LVH-I group; the CVF(4.23％ ± 0.69％) and CVF-Ⅰ (3.92％ ±0.40％) were decreased significantly(P ＜0.01); but CVF-Ⅲ(1.79％ ±0.21％) and Ⅰ/Ⅲ (2.20 ±0.20) did not change significantly in DM LVH-Ⅰ group compared with the EH LVH-Ⅰ group.Conclusions (1)The myocardial stiffness and the reduction of cardiac diastolic function deteriorated most with the development of LVH.(2)Under the condition of the same stage,the myocardial stiffness and the reduction of cardiac diastolic function deteriorated seriously in EH groups,diabetes patients,myocardial stiffness and the reduction of cardiac diastolic function deteriorated slightly.

Objective To investigate the impact of metal plate on radiation dose distribution in surrounding tissues in cadaver specimens.Methods Stainless steel plate, titanium plate, and muscle strip were implanted into the left thigh of a corpse, respectively.All the specimens were irradiated with 6 MV X-ray , SSD = 100 cm.The absorbed dose of surface was measured by thermoluminiscent elements.Results Surface dose distributions differed significantly among the three different materials (F = 57.35, P < 0.01),with the amounts of 1.18 Gy ± 0.04 Gy (stainless steel plate), 1.12 Gy ± 0.04 Gy (titanium plate) and 0.97 Gy ±0.03 Gy (muscle strip), respectively.The surface absorbed doses on incident plane of stainless steel plate and titanium plate were significantly increased by 21.65% and 15.46% respectively as compared with that of muscle strip.The absorbed doses on the exit surface of stainless steel plate, titanium plate and muscle strip were 0.87 Gy ± 0.03 Gy, 0.90 Gy ± 0.02 Gy and 0.95 Gy ± 0.04 Gy, respectively (F =13.37, P <0.01).The doses on the exit surface of stainless steel plate and titanium plate were significantly lowered by 8.42% and 5.26% when compared with that of muscle strip.Using treatment planning system,the differences between dose distribution with and without metal plate were compared.Within 1 cm away from the incident plate, there was an obvious increase in the absorbed dose, while the influence was less than 5% 1cm outside the surface.The effect of dose distribution on exit surface was less than 2%.Conclusions The influence of metal plate on the radiotherapy dose distribution is significant.The deviations ranges from 5% to 29%.Under the same condition, the impact of stainless steel plate is much more than that of titanium alloy plate.

Objective To observe the mechanism of Siwu Decoction (Decoction of four ingredients) on blood deficiency syndrome.Methods Wistar rats were randomized into 3 groups: model group, treatment group, and normal group, 6 ones in each. Rats of the model group and the treatment group were injected 2% acetylphenylhydrazine (APH) saline subcutaneously on the first, forth and seventh day. From the forth day, the model group was treated with saline by gavage, the treatment group was treated with Siwu Decoction by gavage, and the normal group was treated with saline for 7 days in succession. Then the amount of peripheral blood cells, wet weight of liver and spleen were detected. Liver mitochondrial proteome of all rats were extracted. Proteomic technology was used to measure the regulated proteins by Siwu Decoction.Results In the model group, the rats had decreased general status significantly such as pale, enlarged liver and spleen, lowered red blood cells (RBC) and hemoglobin (HGB), increased white blood cells (WBC) and reticulocyte (RCT) (P

Objective To investigates the efficacy and tolerability of second line treatment with S-1 plus thalidomide in patients with advanced pancreatic cancer.Methods Sixty patients hospitalized in Department of Oncology of Cangzhou Central Hospital from July 2010 to October 2012 were included in this study.All the patients were diagnosed as having pancreatic carcinoma.The patients were randomly divided into two groups,one group was treated with S-1 alone,and the other group was treated with S-1 plus thalidomide.Then the efficacy and toxicity of two groups was evaluated.Results The disease control rates were 36.7％ and 46.7％ in the S-1 alone group and the S-1 plus thalidomide group,and the difference between the two groups was not statistically significant (P =0.31).The progression free survival (PFS) was 2.9 months and 3.3 months,and the difference between the two groups was statistically significant (P ＜ 0.05),the Karnofsky score,pain,sleep and weight improvement rate was 63.3％,46.7％,66.7％ and 53.3％ in combination group,which were significantly better than those in control group (30.0％,13.3％,30.0％ and 20.0％),and the difference between the two groups was statistically significant (P ＜ 0.05).The major adverse events were nausea,vomiting,fatigue and drowsiness,mainly of grades Ⅰ ～ Ⅱ.Conclusions S-1 plus thalidomide as second line treatment of pancreatic cancer can prolong the PSF of patients with advanced pancreatic cancer with excellent safety,and patients' quality of life is also improved.

Background and purpose:Thalidomide can enhance the radiation sensitivity on tumor effectively, but the mechanism of radiosensitization is still unclear. The present study aimed to investigate whether thalidomide could enhance the radiation sensitivity on colon cancer transplanted tumor of mouse, and to investigate the underlying mechanism. Methods: We established the model of colon26 colonic carcinoma, and the mice were divided into 4 groups:Control group, the thalidomide group, the radiotherapy group and thalidomide+radiotherapy group. From the day of treatment, tumors were measured every other day. Then, the xenograft tumor growth curve was depicted. Tumor volumes were measured in different treatment groups, then, the inhibitory rates of tumor growth were calcutated. Using immunohistochemical method in to detect the expression of microvessel density (MVD) in tumor tissue. Results:The mean tumor volumes at day 22 were (4.97±1.20)cm3 (control group), (2.90±0.92)cm3 (T group), (2.66±0.88)cm3 (R group), and (1.89±0.76)cm3 (T+R group). The tumor inhibition rate in the combination group (61.9%) was signiifcantly higher than the other groups (41.7%, 46.5%, P<0.05). The radiotherapy sensitization enhancement ratio of the combined treatment group was 2.27 times than in the radiotherapy group. Thalidomide combined with radiation therapy can significantly inhibit microvessel density of tumor:The decreasing MVD of T+R group, T group and R group were respectively 46.8%, 40.7%and 37.7%, and there was statistical significance between T+R group and T group (P<0.05 ), so as between T+R group and R group. It could be found more necrotic cells in tumor of group, and there was statistical signiifcance between T+R group and control group (P<0.05). Conclusion:Thalidomide can enhance the radiosensitivity mice of colonic carcinoma, and its mechanism may be related to the inhibition of tumor angiogenesis related.

Objective To investigate the protective effect of heme oxygenase-1 (HO-1)/carbon monoxide (CO) system on restenosis after balloon angioplasty and relative mechanism. Methods Fifty rabbits were randomly divided into 5 groups. Control group received normal chow (Control group), the other rabbits received 1.5% cholesterol diet (Chol group and SH group) or 1.5%cholesterol diet plus hemin (Hem group) or zinc protoporphyrin Ⅸ (Znpp group) for 10 weeks. At the third week of experiment, the three experimental groups underwent balloon injury at one side common carotid artery. Results Compared with control group, arterial nitric oxide production and nitricoxide synthase activity were significantly decreased, while HO-1 expression and CO production were significantly increased (all P＜0. 01 ) in Chol group. The intima thickness and ratio of intima/media (I/M) were lower in Hem group than in Chol group [(281.47± 21.10) μm vs. (442.17 ±59.14) μm, 2.49 ± 0. 17 vs. 3.99 ± 0. 52, respectively]. While arterial HO-1 expression and CO production were increased markedly, endothelin-1 expression was distinctly reduced in Hem group group than in Chol group. Compared with Chol group, arterial HO-1 expression and CO production were decreased obviously, while endothelin-1 expression and intima thickness and ratio of intima/media [(698.71±58. 37) μm, 6.17±0. 52]were significantly increased in Znpp group (all P＜0. 01).Conclusions The HO-1/CO system plays a protective role on improving endothelium function and restraining neointimal proliferation by compensating and regulating nitricoxide synthase/nitric oxide system and lowering endothelin -1 expression so as to inhibit restenosis after balloon injury.

Objective To study the set-up accuracy in radiotherapy of thoracic neoplasms by improving the body immobilization method.Methods Fifty patients with thoracic neoplasms were randomly divided into conventional group (without electrode paste) and improved group (with electrode paste).Using simulator for position calibration and center field digital image reconstruction from treatment planning system.Then compare the set-up accuracy of two groups with different body methods by grouped t-test.Results Set-up error in the left-right,superior-inferior,anterior-posterior direction were 2.5 ± 1.5 and 2.4 ± 1.4(P =0.010),4.4 ± 2.0 and 2.2 ± 1.2 (P =0.000),2.2 ± 1.3 and 2.1 ± 1.0 (P =0.100) in conventional group and improved group,respectively.Conclusions The improved body immobilization method improves setup accuracy in radiotherapy for thoracic neoplasms which also will be effective for clinical treatment.

Tacrolimus (Tac) is a commonly used immunosuppressant after organ transplantation, which has high immunosuppressive efficacy. However, the pharmacokinetics of Tac significantly differ among individuals, and gene polymorphism is the main influencing factor. In recent years, the gene polymorphism of drug transporter has become a novel research hotspot. Nevertheless, the effect of the gene polymorphism of transporter on Tac pharmacokinetics remains controversial. Consequently, the correlation between the gene polymorphism of transporter and Tac blood concentration plays a significant role in guiding Tac-based individualized immunosuppressive therapy. In this article, the research progresses on the gene polymorphism of adenosine triphosphate-binding cassette (ABC) transporter and solute carrier (SLC) transporter in organ transplantation was reviewed. The correlation between the gene polymorphism of transporter and Tac blood concentration was summarized, aiming to provide reference for Tac-based individualized therapy.