Objective Industrialized breeding technology system will be established to achieve the rapid multiplication of Pinellia ternata and to settle the problem of degeneration caused by seeds. MethodsExplant induction and effects of plant growth regulator dosage, illumination, and subculture cycle on fasciculated shoot propagation were studied. ResultsIt is good for the explant induction on medium MS+6-BA (4-5) mg/L+NAA 0.2 mg/L. P.ternata grows well under the circumstance of NAA 0.2 mg/L+6-BA 2 mg/L, double or more shoots. ConclusionIndustrialized breeding technology system of P.ternata can make its propagation rapid and support an effective way for resources preserving and sustainable utilization.

Objective To explore the application of project management theory in spinal orthopedic nursing personnel training. Methods Project management team was established in Orthopedic Department of West China Hospital based on project management theory in January 2016. Psychology and sleep,nutrition, rehabilitation and other 10 project management teams in total were constructed after strict nursing team training, and nursing personnel training was carried out. Using convenience sampling method,50 nursing staff working in spinal orthopedic department from April to December 2015 were recruited in control group,and 50 nursing staff working from April to December 2016 were recruited in experimental group. On the basis of traditional training mode which was applied in control group,experimental group focused on the project sub-specialty,and then consciously carried out the personnel training work. Compare evaluation of nurses before and after the project management. At the same time,a convenience sampling method was adopted to investigate the satisfaction of 100 discharging patients. Results The understanding of project definition,nursing capacity,nursing work efficiency,patients and their families′ respect for nursing work,nursing research capacity and teamwork ability of experimental group were significantly lower than the control group (P< 0.05). Patient satisfaction improved after carrying out the project management,and the difference was statistical significant (P=0.013). Conclusions The project management theory provides a theoretical basis for nursing personnel training,which has important guiding significance for the training of clinical nursing personnel.

Objective To explore the status of knowledge , attitude and practice in enhanced recovery after surgery (ERAS) among surgical medical staff, and analyze its influencing factors, so as to provide basic information for the multidisciplinary training and education program .Methods A self-designed questionnaire was used to survey 640 surgical medical staff of 17 surgical departments such as osteopathic department , gastrointestinal surgery and neurosurgery in West China Hospital of Sichuan province .The questionnaire included three parts: knowledge , attitude and practice .The SPSS 17.0 statistical software was applied for data analysis and processing.Results The score of ERAS knowledge among medical staff was (39.13±14.23). The score of reducing hospitalization time and costs was (3.30±1.30), which was the highest, while the score of anesthesia was (2.47±1.15), which was the lowest.The score of neurosurgery department was the lowest and was (25.35±12.82), while the score of general surgery was the highest and was (46.12±12.83). As for the ERAS attitude and practice of medical staff , 93.91%of medical staff agreed that ERAS could enhance patients′recovery after surgery .99.06%and 98.13%of the medical staff were willing to participate in the related study and research .41.41%of staff recently had access to relevant knowledge within one month.The most important way of acquiring knowledge was communication between colleagues and lectures . Single factor analysis showed that:there were significant difference in ERAS knowledge score among medical staff with different departments, occupation, age, job title, highest education and working experience (P<0.01).Conclusions The ERAS knowledge score of surgical medical staff is low , and their clinical practice doesn′t match their attitude .Departemnts should organize targeted ERAS training according to different characteristics of medical staff among medical staff .The ERAS should be applied into the clinical practice and better the service for patients .

OBJECTIVE: To explore the genetic basis for a child with developmental delay and congenital syndactyly. METHODS: G-banding chromosomal karyotyping and chromosomal microarray analysis (CMA) were performed on peripheral blood sample from the child. RESULTS: The child was ascertained as 46, XY, r(18)[52]/45,XY,?18[3]. A 18q21.32-q23 deletion was identified by CMA with a size of 19.85 Mb, which has encompassed 99 genes including CTDP1, TXNL4A, TSHZ1, PIGN, RTTN, TNFRSF11A, KDSR and CYB5A. CONCLUSION Clinical phenotype of the patient with ring chromosome 18 is associated with the size of the euchromatin loss and involved genes. As a useful complement to conventional karyotyping, CMA has provided an powerful tool for delineating complex chromosomal aberrations.
Child ; Chromosome Aberrations ; Chromosomes, Human, Pair 18 ; genetics ; Cytogenetics ; Developmental Disabilities ; genetics ; Humans ; Karyotyping ; Ring Chromosomes ; Syndactyly ; genetics

Objective:To determine the contents of quercetin, kaempferol, total flavonoids and extracts in 52 samples of Lysimachiae Herba collected from different origins; To analyze the quality differences of Lysimachiae Herba among different producing areas. Methods:The quercetin and kaempferol contents of the Lysimachiae Herba from Guizhou Province, Sichuan Province and Chongqing were determined by HPLC, and the total flavonoids were determined by Symergy HTX microplate reader. Results:The total content of quercetin and kaempferol in 52 samples was among 0.146 2-2.517 0 mg/g, with an average content of 0.872 6 mg/g, among which the average content of Sichuan was 1.073 2 mg/g, that of Guizhou was 0.705 4 mg/g, and that of Chongqing was 0.865 1 mg/g. Among them, 20 samples reached the standard of the Chinese Pharmacopoeia. The average content of the samples that met the standard was 1.439 7 mg/g. The compliance rate of samples collected in Guizhou, Sichuan and Chongqing reached 12.5%, 62.5%, and 38.8% respectively. The total flavonoid content of 52 samples was among 0.994 2- 3.866 4 mg/g, and 52 samples were in conformity with the ethanol hot extract standard of the Chinese Pharmacopoeia. Conclusions:The total contents of quercetin and kaempferol from different sources in Sichuan, Guizhou and Chongqing are quite different, and the total contents of quercetin and kaempferol collected from the same district and county are also quite different, and the compliance rate is low. There are great differences in total flavonoids in different producing areas and different populations of Lysimachiae Herba samples collected in the field.

Objective: To explore the genetic basis for a child with developmental delay and congenital syndactyly. Methods: G-banding chromosomal karyotyping and chromosomal microarray analysis (CMA) were performed on peripheral blood sample from the child. Results: The child was ascertained as 46, XY, r(18)[52]/45, XY, ? 18[3]. A 18q21.32-q23 deletion was identified by CMA with a size of 19.85 Mb, which has encompassed 99 genes including CTDP1, TXNL4A, TSHZ1, PIGN, RTTN, TNFRSF11A, KDSR and CYB5A. Conclusion Clinical phenotype of the patient with ring chromosome 18 is associated with the size of the euchromatin loss and involved genes. As a useful complement to conventional karyotyping, CMA has provided an powerful tool for delineating complex chromosomal aberrations.