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Dendrobium officinale is a kind of precious Chinese herbal medicine, which has good effects on the anti-aging, antineoplastic, and blood glucose reducing treatment. It has been listed in the top of “Nine Magic Herbs”, with significant cultural features of traditional Chinese medicine (TCM). Planting, processing, and cultural base construction of Dendrobium officinale in Hunan Province have obtained great achievements. Further combination of Dendrobium officinale with TCM culture will develop a series portable and convenient tourism products with the features of wide taking habits and large intended population. The planting of Dendrobium officinale should be integrated with ecofallow organically; the construction of science museums about Dendrobium officinale should be improved; the modern view on health maintenance “Health maintenance should focus on Yin nourishment” should be included in TCM cultural tourism in Hunan Province. All of these can improve the potential of tourism product development, meet the living requirements of modern people, and drive the development of tourist industry in Hunan Province.

Objective:To study the impact of perioperative intestinal microecological intervention on abdominal infection after liver transplantation.Method:Fifty patients who underwent liver transplantation at the Department of Hepatobiliary Surgery, Ningbo Medical Center Lihuili Hospital from June 2017 to June 2019 were retrospectively studied. There were 34 males and 16 females. The average age was 47 years. These patients were divided into two groups using the method of non-randomized concurrent control. One group formed the perioperative intestinal microbiological intervention group, while the other group served as the conventional treatment control group. There were 25 patients in each group. The preoperative and postoperative intestinal flora and the occurrence of postoperative abdominal infection of the two groups were compared.Results:After operation in both groups, enterococci increased, enterobacteriaceae decreased, while non-fermenting bacteria and candida showed no significant changes. In the intervention group, the counts of enterobacter, enterococcus and candida were significantly lower than the control group on day 15 after operation ( P<0.05). The contents of bifidobacteria and lactobacillus in the intervention group were significantly higher than that in the control group on days 7 and 15 after operation. On the other hand, the contents of enterobacter on day 7 and enterococcus on day 15 after operation in the intervention group were significantly lower than the control group ( P<0.05). The intestinal flora of the two groups were imbalanced before liver transplantation. The ratio of bifidobacterium / enterobacteriaceae in the intervention group was significantly higher than the control group on day 7 and 15 after operation ( P<0.05). The incidence of intestinal bacterial translocation in the control group was 32.0% (8/25), with 6 patients diagnosed to have abdominal infection caused by intestinal bacterial translocation. The incidence of intestinal bacterial translocation was 8% (2/25) in the intervention group, with 1 patient diagnosed to have abdominal infection caused by intestinal bacterial translocation. The incidence of bacterial translocation in the intervention group was significantly lower than the control group ( P<0.05). There were no significant differences in the incidences in the intestinal bacterial translocation and abdominal infection between the two groups ( P>0.05). Conclusions:Probiotics intervention in the perioperative period of liver transplantation improved the imbalance of intestinal flora, reduced the incidence of intestinal bacterial translocation, and played a positive role in reducing abdominal infection after liver transplantation.

OBJECTIVE: To investigate the expression of Toll-like receptor 4 (TLR4)/myeloid differentiation factor (MyD88)/nuclear factor-κB (NF-κB) pathway genes and related inflammatory factors tumor necrosis factor-α (TNF-α), interleukin (IL)-12, IL-6 in patients with secondary spinal cord injury (SSCI) and the correlations with prognosis. METHODS: The clinical data of 105 SSCI patients and 40 healthy subjects were reviewed. According to Frankel's classification of spinal cord injury, the patients were divided into complete injury group and incomplete injury group, and according to the improvement of Japanese Orthopedic Association (JOA) scores, the patients were divided into good prognosis group and poor prognosis group. The expression of TLR4, MyD88, NF-κB in peripheral blood mononuclear cells (PBMC) and serum TNF-α, IL-12, IL-6 levels were compared between SSCI patients and healthy controls, between patients with complete and incomplete injury, between patients with poor and good prognosis. Logistic regression analysis was used to analyze the risk factors leading to poor prognosis of SSCI, and Pearson's correlation analysis was used to analyze the correlation between JOA score and the above indicators. RESULTS The expressions of TLR4, MyD88, NF-κB in PBMC and serum TNF-α, IL-12, IL-6 levels in SSCI patients were significantly higher than those in healthy subjects (all P<0.01), those in complete injury group were higher than those in incomplete injury group, and those in poor prognosis group were higher than those in good prognosis group (all P<0.01). The proportions of patients with Frankel grade A, spinal cord edema or hemorrhage, spinal cord injury length longer than 4 cm in poor prognosis group was significantly higher than those in good prognosis group (all P<0.01). Logistic regression analysis showed that Frankel grade, spinal cord edema or hemorrhage, length of spinal cord injury, relative expressions of TLR4, MyD88, NF-κB in PBMC, serum levels of TNF-α, IL-12 and IL-6 were risk factors for poor prognosis in SSCI patients (P<0.05 or P<0.01). Pearson's correlation analysis showed that JOA improvement rate was negatively correlated with the relative expressions of TLR4, MyD88, NF-κB mRNA in PBMC and serum TNF-α, IL-12, IL-6 levels (P<0.05 or P<0.01). CONCLUSIONS The activation of TLR4/MyD88/NF-κB pathway and the up-regulation of the expression of related inflammatory factors TNF-α, IL-12 and IL-6 are involved in the progression of SSCI, which are closely related to the neuroinflammatory injury, and can be used as reference indexes for evaluating prognosis in SSCI patients.